- Open Access
Co-expression of NMDA-receptor subunits NR1, NR2A, and NR2B in dysplastic neurons of teratomas in patients with paraneoplastic NMDA-receptor-encephalitis: a retrospective clinico-pathology study of 159 patients
Acta Neuropathologica Communications volume 8, Article number: 130 (2020)
To comprehensively describe the pathological features of neurons in patients with ovarian teratomas and paraneoplastic anti-NMDAR encephalitis (anti-NMDARE), emphasizing on NMDA-receptor expression and infiltrating lymphocytes.
A retrospective study was performed in a large series of 159 patients from the West China Hospital. We retrospectively identified 12 patients with paraneoplastic anti-NMDARE (11 case with ovarian teratomas and 1 case with mixed germ cell tumor), which were compared to 35 patients with teratomas and no encephalitis and to 147 patients with anti-NMDARE and no evidence for tumors. Patient history and outcome were reviewed from the clinical charts and compared between all three groups. Histopathological examination, including double-immunofluorescence of NMDAR subunits and IgG was performed in all teratoma tissues. Magnetic Luminex Assay Human Premixed Multi-Analyte Kit was performed to investigate cytokines profile of CSF.
Patients with paraneoplastic anti-NMDARE had a more severe clinical presentation, i.e. they required more mechanical ventilation and intensive care (p < 0.001). Though immunotherapy was initiated earlier in this group, repeated intravenous immunoglobulin administration (IVIG) was more common (p = 0.002) and with higher cerebrospinal fluid (CSF) antibody titres (p = 0.004). Following tumor resection, the outcome did not differ between groups. A peculiar population of floating-frog like dysplastic neurons were observed only in teratomas of patients with paraneoplastic anti-NMDARE, co-expressing NR1, NR2A, NR2B subunits and IgG. Also, CD20 positive B-cells were more common in anti-NMDARE teratomas. In CSF of paraneoplastic anti-NMDARE patients, TNF-α, IL-10 and GM-CSF concentrations were higher than in negative symptom control and VEGF-A and IL-1a were lower than in anti-NMDARE patients (0.25 < p < 0.05).
Patients with teratomas and paraneoplastic anti-NMDARE revealed a cellular population of dysplastic neurons co-expressing NMDAR subunits, which were the potential source of autoantigens triggering anti-NMDARE. Some inflammatory cytokines may be involved in pathogenesis of paraneoplastic anti-NMDARE.
Anti–N-methyl-D-aspartate receptor encephalitis (anti-NMDARE) is a major type of autoimmune encephalitis with more than 1000 reported cases since the discovery of autoantibodies against the NR1 subunit of the NMDA receptor in 2007 [9, 24]. In 4106 cases of encephalitis of unidentified aetiology in China, 423 cases (10.3%) were positive for anti-NMDAR antibodies . Anti-NMDARE is also considered a paraneoplastic syndrome. Tumors, especially ovarian teratomas, exist in 38–59% of cases with anti-NMDARE in western countries [8, 19]. In China, ovarian teratomas were observed in 8% of all patients and 26.9% of women with anti-NMDARE [6, 22]. It has been suggested that anti-NMDAR antibodies were directed against neuronal cell surface antigens of specific tumor cell populations, i.e. dysplastic neurons, triggering the onset of anti-NMDARE with severe neurological symptoms following CNS distribution via cerebrospinal fluid (CSF) . Previous studies described also abundant lymphocyte infiltration in teratoma tissues [5, 13, 20], suggesting that an inflammatory response is caused by these teratomas. Though brain tissue is difficult to obtain from such patients, histopathological specimens from resected teratomas are a valuable source for analysis in order to better understand disease pathomechanism. Our current study aimed to explore the relationship between teratomas and anti-NMDARE by systematically reviewing pathology, clinical features, and inflammatory cytokines.
Materials and methods
Between February 2013 and October 2017, 3020 patients were admitted with clinical suspicion of autoimmune encephalitis to the West China Hospital in Chengdu. Serum and CSF samples were taken from each patient and sent to Peking Union Medical College Hospital (China) for screening of autoimmune-antibodies, including antibodies to NMDAR, contactin-associated protein-like 2 (CASP2), leucine-rich glioma inactivated 1 (LGI1), gamma aminobutyric acid beta receptor (GABAB-R), glutamic acid decarboxylase (GAD65), alpha-amino-3-hydroxy-5-methyl-4-isoxazole -propionic acid 1/2 receptor (AMPA1/2-R), IgLON5, dipeptidyl-peptidase-like protein-6 (DPPX), metabotropic glutamate receptors (mGLURs), voltage gated potassium channel (VGKC), and voltage gated calcium channel (VGCC). All serum and CSF specimens were evaluated by indirect immunofluorescence using EU 90 cells transfected with the NMDAR1 subunit (NR1) of the NMDAR complex and immobilized on BIOCHIPs (Euroimmun AG, L €ubeck, Germany) as previously described . Finally, a diagnosis of anti-NMDARE was confirmed in 159 patients . Antibody titres in CSF and serum were classified as weakly positive (CSF 1:1, serum 1:10), positive (CSF 1:10 or 1:32, serum 1:32), and strongly positive (CSF 1:100 or 1:320, serum 1:100). Twelve of the patients with anti-NMDARE, who suffered from a teratoma and received operation, were included into the study (the paraneoplastic anti-NMDARE group). In addition, we identified 64 age-matched patients (age range 17 to 43 years) without neurological dysfunction who had teratomas resected between Jun 1st and Jun 30th in 2018 in the Department of Gynecology, West China Second Hospital, Sichuan University. Histopathologically, a total of 35 teratomas revealed central nervous tissues and these samples were selected as a control group. In addition, patients with anti-NMDAE were included as the anti-NMDAE controls (N) and patients who diagnosed as chronic headache with normal MRI and CSF test were included as the negative symptom controls (C) for CSF cytokines and chemokines detection.
Clinical data review
Details of the demographics, clinical manifestations, results of auxiliary examinations, treatment strategies, and outcomes were reviewed from the hospital archives. A modified Rankin Scale (mRS) was used to evaluate the general outcome at the final follow-up. A good outcome was defined as mRS score 0–2 . Relapse of encephalitis was defined as new onset or worsening of symptoms occurring after an initial improvement or stabilization of at least 2 months .
After CSF were obtained during the acute phase of encephalitis, all samples were centrifuged at 1000 r/min for 10 min. Subsequently, the supernatants were stored at − 20 °C for up to 1 month and then in liquid nitrogen until further use.
Formalin-fixed and paraffin-embedded blocks of tumor tissue were obtained from the Department of Pathology, West China Hospital/ West China Second Hospital. Four μm thin sections were cut with a microtome and used for further analysis (see below).
For every tumor block, five sections were cut and reviewed by Hematoxylin-Eosin (HE) staining and immunohistochemistry of Neuronal Nuclear epitope (NeuN), Microtubule Associated Protein 2 (MAP2), S100 (S100), and Glial Fibrillary Acidic Protein (GFAP). The tumor blocks with confirmed central nervous system tissues were prepared for next step of experiment. All sections were reviewed using Hematoxylin-Eosin (HE) staining and further processed for immunohistochemistry and immunofluorescence double-staining using standardized protocols. Lymphocytes were detected with antibodies directed against CD20 specific for B-cells, CD138 for plasma cells and against CD3, CD4 and CD8 for T-cells. Antibodies directed against immunoglobulin G (IgG), NR1, NR2A, and NR2B were used as specified in Supplementary Table 1. Thirty sections were cut from every tumor and six sections from the 1st, 7th, 13th, 19th, 25th and 30th section was chosen for NR1/NR2A/NR2B immunofluorescence. To identify the cellular source of IgG binding, immunofluorescence double-staining was performed with NR1/NR2A/NR2B receptors. FITC (fluorescein isothiocyanate) and TMRITC (trimethyl rhodamine isothiocyanate) were used to label IgG and NR1/NR2A/NR2B receptors, respectively, and visualized with a fluorescence microscope (Zeiss, AX10 imager A2/AX10 cam HRC). We observed the whole field and screened five surface per section fluorescence of co-localization after merging. Yellow co-localization cells were evaluated under 400 multiple.
The immunohistochemical and double immunofluorescence results were independently evaluated by one pathologist and two neurologists who were blinded to all experimental groups.
Chemokine and cytokine detection in CSF
We applied the Magnetic Luminex Assay Human Premixed Multi-Analyte Kit (R&D Systems, Minneapolis, MN, USA) to measure the concentration of the following chemokines and cytokines (according to the manufacturer’s instructions): chemokines C-C motif chemokine ligand 2 (CCL2), C-X-C motif chemokine ligand 1 (CXCL1), CXCL9, CXCL10 and CXCL13, and the cytokines interferon (IFN)-α, IFN-γ, interleukin (IL)-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, IL-17A, tumour necrosis factor (TNF)-α, vascular endothelial growth factor A (VEGF-A), a proliferation-inducing ligand (APRIL), B-cell-activating factor belonging to the tumour necrosis factor family (BAFF), granulocyte colony-stimulating factor (G-CSF), and granulocyte-macrophage colony-stimulating factor (GM-CSF). All data were collected with the Luminex-100 system (Luminex, Austin, TX, USA).
Statistic Package for Social Science (SPSS) version 22.0 (SPSS Inc. Chicago, IL, USA) was used for statistical analyses. The cytokine/chemokine levels of paraneoplastic anti-NMDARE patients were compared with anti-NMDARE patients and negative symptom controls respectively by the Mann-Whitney U test and Bonferroni correction (P < 0.025 was considered as significant). Mann-Whitney U test was also applied for other continuous variables that were not normally distributed and the chi-square test or Fisher’s exact test for categorical variables. Differences with a p-value < 0.05 were considered as significant.
Clinical features and demographics
A total of 159 patients were included in this study and divided into paraneoplastic anti-NMDARE and anti-NMDARE groups. The study group (12 cases) included 11 women and 1 man, and the median age was 25 years (range: 17–43). In these patients, the first five common symptoms were seizures (12/12), cognitive dysfunction (12/12), psychiatric behaviors (11/12), decreased levels of consciousness (10/12), and central hypoventilation (8/12). The median length of hospital stay of these patients was 57 days (range: 15–385). Electroencephalography (EEG) showed slow waves and/or epileptiform discharges as well as extreme delta brushes in 11 patients. Neuroimaging results showed abnormal signaling in T2 or fluid-attenuated inversion recovery (FLAIR) in the cortex or the white matter, meningeal enhancement, and ventricle enlargement in 5 patients. Routine CSF tests showed elevated pressure (> 180 mm H2O) and a slightly elevated white blood cell count (> 5/μl) and/or increased proteins (> 500 mg/L) in six patients. Immunotherapy including IVIG, methylprednisolone and rituximab were used in addition to tumor resection in all patients. Two cases received chemotherapy (VBC: vincristine, bleomycin, and cis-platinum) after the histopathology diagnosis revealed an immature teratoma. The median follow-up duration was 26 months (range 1–66 months). Eleven patients (91.7%) had a good outcome (mRS 0–2) and 1 patient died. This patient (case 12) suffered from fast tumor growth and pulmonary metastases, and died of respiratory failure in the third month after onset. All demographic and clinical data were summarized in Table 1.
Comparisons of clinical features between the paraneoplastic anti-NMDARE and anti-NMDARE groups showed that teratomas occurred more often in women than men (91.7% vs 53.1%; p = 0.01). The paraneoplastic anti-NMDARE group received more often central hypoventilation compared to the anti-NMDARE group (66.7% vs 17.0%; p < 0.001), required more intensive care (58.3% vs 8.8%, p < 0.001) and the length of their hospital stay was longer (57.5 days vs 24 days; p = 0.001). The paraneoplastic anti-NMDARE group also had higher CSF antibody titres (p = 0.004). Though the paraneoplastic anti-NMDARE group received immunotherapy earlier (interval from onset to immunotherapy: 12 days vs 25 days, p = 0.002), repeated immunotherapy was more commonly used than in the control group (2 times IVIG vs 1 time IVIG, p = 0.002). However, after tumor resection, the final outcome and relapse rate did not differ between the two groups. Comparisons of clinical features between the paraneoplastic anti-NMDARE and anti-NMDARE groups were summarized in Table 2.
The 12 patients with anti-NMDARE presented with the following spectrum of histopathology diagnosis: mature teratomas of the ovary (case 2–9), mature teratoma of the mediastinum (case 1), immature teratomas of the ovary (WHO III, cases 10–11), and a mixed germ cell tumor of the mediastinum (case 12). The size of the teratomas ranged from 2.1 × 2 × 1.9 cm to 18.5 × 10.3 × 9 cm in patients with anti-NMDARE (Table 3) and from 2 × 1.8 × 1 cm to 16.5 × 13 × 8.5 cm for the control group (Supplementary Table 2). HE staining revealed a characteristic spectrum of mature elements including skin, gastrointestinal tissue, muscle, cartilage, and sebaceous tissue accompanied by mature or immature neural elements.
NeuN staining of neural tissue in the tumor study group showed following patterns (Table 4): (I) neural tissues with normal mature neurons; (II) dysmorphic neurons with floating-frog neural elements; (III) clusters of dysmorphic neurons; (IV) scattered dysplastic neurons; (V) small cells with enlarged nuclei positive for NeuN in 1 case (N12). The dysmorphic neurons showed an irregular cell shape with giant nuclei. Eleven of the 12 cases (91.7%) also tested positive for MAP2 and S100. Pathological findings of neural tissues in teratomas from patients with NMDARE and their staining for NR1, NR2A and NR2B were shown in Fig. 1.
Inflammatory cell infiltrates were composed of CD20- and CD3-positive lymphocytes. The lymphocytes usually clustered in nodules with intensive staining for CD20-positive B cells at the center, and CD3 positive T-cells at the periphery. In teratomas with anti-NMDARE, inflammatory cells with a CD20-positive lymphocytic wall adjacent to the neuropil were seen in 1 case. CD20-positive lymphocytic walls of 2–3 layers around the vasculature were found in 2 cases. More CD20-positive B-cells and less CD4-positive helper T-cells were present in the teratomas associated with anti-NMDARE than in the controls (CD20: p = 0.001, CD4: p = 0.009, Table 3 and Supplemental Table 2), but there was no statistical difference regarding CD138-positive plasma cells, CD3-positive T-cells and CD8-positive cytotoxic T-cells between the two groups (CD138: p = 0.521; CD3: p = 0.293; CD8: p = 0.386).
In the control group, all cases showed neural tissues consisting of mature neurons. One cases (C12) had an additional small pitch of dysplastic neurons. Mature neurons in twenty cases stained positive for NMDAR subunits expression (NR1: n = 9; NR2A: n = 16; NR2B: n = 11) while fifteen cases were negative for NR1, NR2A or NR2B immunoreactivity. Interestingly, dysplastic neurons in C12 did not show any NR1, NR2A or NR2B positivity. GFAP expression was positive in all patients from the study and control groups. Figure 2 summarized the pathological features of the control group. Ten of 12 samples of patients with paraneoplastic anti-NMDARE showed moderate or intense expression of NR1 and NR2A /NR2B subunits, while N3 was positive only for NR2A and NR2B and N9 was positive only for NR1 and NR2B. NR1, NR2A and NR2B expression was stronger in the anti-NMDARE group than in the control group (NR1: p < 0.001; NR2A: p < 0.001; NR2B: p < 0.001, Table 4 and Supplemental Table 3). There was no significant difference between the two groups regarding the expression of IgG (p = 0.069, Table 3 and Supplemental Table 2). In the encephalitis associated teratomas with dysmorphic neurons, all NMDAR subunits and IgG were detected in the same visual field. Subsequent immunofluorescence staining showed IgG reactivity substantially co-localized with the reactivity of NR1, NR2A, and NR2B antibodies in patients with paraneoplastic anti-NMDARE. In the control group, no sample exhibited NR1, NR2A and NR2B reactivity with IgG (Fig. 3).
Case N12 was diagnosed with a mixed germ cell tumor (choriocarcinoma with teratoma) and the patient died of multiple organ failure within 3 months. The sections from this patient showed very different pathological characteristics to other patients (Fig. 4). On HE sections, tumor cells formed an intensive cluster with increased mitosis. NeuN-positive cells had an atypically closed neuronal shape with high density and irregular nuclei. MAP2- and S100-positive cell staining was diffuse. Lymphocytes clustered as foci with numerous intensive CD20 and CD138-positive cells, while intensive CD3-positive cells were separately distributed around neurons. Moderate CD4, CD8-positive, NR2A-positive cells, strong staining of diffusing IgG and clustered NR2B-positive cells were observed. Cell necrosis was also obvious in this sample. Other immunolabelled results included PLAP(+, focal), OCT3/4(−), glypican-3(−), CD117(−), CD30(−), AFP(−), HCG(+), HPL(+, focal), CK(+), and EMA(−) (Fig. 4).
Chemokines and cytokines in CSF
As shown in Table 5, we detected higher levels of TNF-α, IL-10 and GM-CSF in CSF of paraneoplastic anti-NMDARE patients compared to the negative symptom control group (P < 0.05)and lower level of IL-1α and VEGF-A compared to the anti-NMDAR encephalitis patients(P < 0.05). However, there was no statistically significant difference after Bonferroni correction as the comparison were performed twice.
Ovarian teratomas are the most common tumors in patients with paraneoplastic anti-NMDARE. The penetrance is reported to be 94% and mainly affects young women . Our hospital-based demographic data showed lower occurrence rate of women (56%) and teratomas (7.5%) than reported in other regions (91, and 58%, respectively) . This may be due to our different ethnic and gender ratio, however, patients with paraneoplastic anti-NMDARE presented a more severe illness than anti-NMDARE patients without tumors. Our data demonstrated a higher percentage of mechanical ventilation, intensive care, higher CSF antibody titres, and the late response to immune-therapy. Our data also showed that complete tumor resection and sufficient immune-therapy are important for successful outcome. This is consistent with a previous study showing a significantly better outcome in patients with early resection of teratomas compared to late or no resection .
Previous studies showed that tumors, especially teratomas, in patients with anti-NMDARE contained nervous tissues reacting with patients’ antibodies  and contained larger amounts of inflammatory infiltrates compared to teratomas from patients which did not develop anti-NMDARE . These findings suggested that the teratomas were directly related to the pathogenesis of anti-NMDAR encephalitis. However, neural tissues are common in any teratoma, also in patients which do not present with symptoms of anti-NMDARE . We carefully compared, therefore, the histopathology findings in teratomas obtained from different patient groups and found a peculiar population of dysmorphic neurons present in teratomas of patients with anti-NMDARE (10/11) but rarely of patients without anti-NMDARE (1/35). These dysmorphic neurons appeared irregular in their cell shape and had giant nuclei. They clustered in aggregates or scattered diffusely throughout the tumour tissue. A similar pathology finding was reported by Gregory et al. who showed that gangliogliomas, ganglioneuroblastoma, and neuronal abnormalities synonymous for the herein described population of dysmorphic neurons were absent from the control group . In our control group, all cases had normal (mature) neurons, with the exception of one case also showing aggregates of dysmorphic neurons.
Our results showed that 90% of encephalitis-related teratomas were immunoreactive for antibodies directed against NR2A and NR2B epitopes. Specifically, the dysmorphic neurons in these teratomas had strong immunoreactivity for NMDAR subunits NR1, NR2A, and NR2B, and subsequent immunofluorescence showed consistent co-localisation of NMDAR subunits with IgG. In contrast, the neurons in most control cases showed negative or weakly positive for NMDAR subunits and no IgG-NMDAR co-localization, supporting the notion that aberrant IgG-NMDAR subunit expression in teratomas play an important role in the pathogenesis of anti-NMDARE [2, 3, 15, 18].
In the mixed germ cell tumours of case 12, normal neurons were replaced by a high density of cells with an atypical neuronal shape that stained positively for NeuN. Co-localization of IgG-NR2B was also detected. The results indicated that these cells may be the origin of NMDAR antibodies that lead to encephalitis. Though the tumor was resected, the patient quickly presented pulmonary metastasis and subsequently died of multiple organ failure. This case is very rare but demonstrates that even in malignant mixed tumours, neural cells expressing NMDA receptors are likely a key factor underlying the pathogenesis of anti-NMDARE.
Previous studies showed lymphocytes infiltrating into neural tissue of teratomas [5, 13, 20]. Though the adjacent localization of both cell components was not found in this study, inflammatory infiltrates were observed more frequently in cases with anti-NMDARE than controls, and CD20 labelled B-cells were predominant, further suggesting a humoral immune environment in the teratomas. Indeed, Mateusz et al. described that infiltrating lymphocytes in anti-NMDARE associated ovarian teratoma tissue could produce NR1-IgG in cell culture .
Although there was no statistical difference, it showed increasing trends of TNF-α, IL-10 and GM-CSF in patients with paraneoplastic anti-NMDARE than the negative symptom control, which indicated the involvement of T cell in pathogenesis of paraneoplastic anti-NMDARE. The result is similar to Kothur’s study . Especially, the role of GM-CSF in autoimmune and inflammatory diseases gained rising attention recently. Increased levels of GM-CSF not only upregulate further production of proinflammatory cytokines from macrophages or dendritic cells, it also forms positive feedback, activates microglial cells, produces highly neurotoxic substances . Moreover, TNF-α have a moderating effect on blood brain barrier (BBB) permeability via the internalization of tight junction proteins on endothelial cells  and GM-CSF is required for recruitment of peripheral myeloid cells that contribute to blood-brain and blood-spinal cord barriers disruption , which increases permeability and facilitates the transfer of serum antibodies through the BBB to the CSF. VEGF-A and IL-1α were higher in CSF of patients with anti-NMDARE but no tumor compared to those with paraneoplastic anti-NMDARE may suggest a different inflammation process.
We observed a lower occurrence rate of anti-NMDARE in Chinese patients with teratomas compared to other regions. However, patients with paraneoplastic anti-NMDARE presented clinically with a more serious illness than patients with anti-NMDARE and no tumours. We identified a peculiar cell population of dysmorphic neurons in teratomas with co-expression of NR1/NR2A/NR2B and IgG, and these neurons were candidate targets for autoimmune antibodies. Our current results will require further investigations, however, to experimentally confirm the role of the dysmorphic neurons with a floating-frog like appearance in paraneoplastic anti-NMDARE and the role of activating inflammatory cytokines.
Availability of data and materials
The datasets used and/or analysed during the current study available from the corresponding author on reasonable request.
- anti-NMDAR E:
Anti–N-methyl-D-aspartate receptor encephalitis
Contactin-associated protein-like 2
Leucine-rich glioma inactivated 1
Gamma aminobutyric acid beta receptor
Glutamic acid decarboxylase
Alpha-amino-3-hydroxy-5-methyl-4-isoxazole- propionic acid 1/2 receptor
Metabotropic glutamate receptors
Voltage gated potassium channel
Avoltage gated calcium channel
Modified Rankin Scale
Neuronal Nuclear epitope
- MAP 2:
Microtubule Associated Protein 2
Glial Fibrillary Acidic Protein
Trimethyl rhodamine isothiocyanate
C-C motif chemokine ligand
C-X-C motif chemokine ligand
Tumour necrosis factor
Vascular endothelial growth factor A
A proliferation-inducing ligand
B-cell-activating factor belonging to the tumour necrosis factor family
Granulocyte colony-stimulating factor
Granulocyte-macrophage colony-stimulating factor
Statistic Package for Social Science
Fluid-attenuated inversion recovery
Placental alkaline phosphatase
Alpha fetal protein
Human chorionic gonadotropin
Human placental lactogen
Epithelial membrane antigen
Blood brain barrier
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We gratefully acknowledge Dr. Li Li and Dr. Fei Chen from Pathology laboratory of Sichuan University for excellent technical support, as well as Le Xiong, Xin Gao and Xiang Hu for help and suggestions.
This work was supported by grants from National Natural Science Foundation of China (Grant No. 81571272) and Sichuan province science and technology project (Grant No. 2019YFH0145).
Ethics approval and consent to participate
This study was approved by the Ethical Review Board of West China Hospital, Sichuan University (ChiCTR1900027074). Written informed consent was obtained from the patients or his/her direct relative for undergoing lumbar puncture, tumour resection, and the use of samples for research analysis.
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Additional file 1: Table S1-3.
Showed primary antibodies used in immunohistochemistry and immunofluorescence in the experiment; surgery finding, inflammatory features and NMDAR subunits expression in teratomas from patients without anti-NMDARE.
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Jiang, XY., Lei, S., Zhang, L. et al. Co-expression of NMDA-receptor subunits NR1, NR2A, and NR2B in dysplastic neurons of teratomas in patients with paraneoplastic NMDA-receptor-encephalitis: a retrospective clinico-pathology study of 159 patients. acta neuropathol commun 8, 130 (2020). https://doi.org/10.1186/s40478-020-00999-2
- Dysplastic neurons
- NMDA receptor