- Open Access
Pharmacological inhibition of HDAC6 reverses cognitive impairment and tau pathology as a result of cisplatin treatment
© The Author(s). 2018
- Received: 18 September 2018
- Accepted: 21 September 2018
- Published: 1 October 2018
Chemotherapy-induced cognitive impairment (CICI) is a commonly reported neurotoxic side effect of chemotherapy, occurring in up to 75% cancer patients. CICI manifests as decrements in working memory, executive functioning, attention, and processing speed, and greatly interferes with patients’ daily performance and quality of life. Currently no treatment for CICI has been approved by the US Food and Drug Administration. We show here that treatment with a brain-penetrating histone deacetylase 6 (HDAC6) inhibitor for two weeks was sufficient to fully reverse cisplatin-induced cognitive impairments in male mice, as demonstrated in the Y-maze test of spontaneous alternation, the novel object/place recognition test, and the puzzle box test. Normalization of cognitive impairment was associated with reversal of cisplatin-induced synaptosomal mitochondrial deficits and restoration of synaptic integrity. Mechanistically, cisplatin induced deacetylation of the microtubule protein α-tubulin and hyperphosphorylation of the microtubule-associated protein tau. These cisplatin-induced changes were reversed by HDAC6 inhibition. Our data suggest that inhibition of HDAC6 restores microtubule stability and reverses tau phosphorylation, leading to normalization of synaptosomal mitochondrial function and synaptic integrity and thereby to reversal of CICI. Remarkably, our results indicate that short-term daily treatment with the HDAC6 inhibitor was sufficient to achieve prolonged reversal of established behavioral, structural and functional deficits induced by cisplatin. Because the beneficial effects of HDAC6 inhibitors as add-ons to cancer treatment have been demonstrated in clinical trials, selective targeting of HDAC6 with brain-penetrating inhibitors appears a promising therapeutic approach for reversing chemotherapy-induced neurotoxicity while enhancing tumor control.
- Chemotherapy-induced cognitive impairment
- Synaptic integrity
The success of cancer treatment has increased dramatically over the past decade, with the survivor population reaching 15.5 million in the US alone and 28 million worldwide in 2016 [14, 22]. However, the quality of life of cancer survivors is often affected by serious chemotherapy-induced neurotoxic side effects, including chemotherapy-induced cognitive impairment (CICI), also termed “chemobrain” [26, 49]. CICI is reported by up to 75% patients treated for cancer and manifests as decrements in working memory functioning, executive functioning, attention, and processing speed [17, 26]. Prospective neuroimaging fMRI studies have indicated hypoactivation of various brain regions, and diffusion tensor imaging analysis has indicated structural changes in white-matter areas of the brain in patients treated for cancer [16, 28, 36]. To date, no preventive or curative intervention for CICI has been approved by the US Food and Drug Administration. We have therefore developed a model of cisplatin-induced cognitive deficits in the mouse to allow investigation of the mechanism and development of curative interventions for CICI.
A major concern in developing interventions for chemotherapy-induced neurotoxicities is that they might interfere with tumor control. An absolute requirement for the treatment of CICI would be to exclusively use agents that do not interfere with, and preferably even enhance tumor control. In this regard, agents targeting histone deacetylase 6 (HDAC6) are promising candidates to fill this void. HDAC6 is a unique member of the histone deacetylase family, as it does not interact with histones in vivo . Its primary targets include non-histone cytoplasmic substrates, such as the microtubule protein α-tubulin and heat shock protein 90 (HSP90) [25, 30]. Inhibitors of HDAC6 display anti-tumor activity in various cancer models; thus, HDAC6 inhibition is being considered as an add-on treatment to inhibit tumor growth [1, 2, 24, 46]. One of the HDAC6 inhibitors, ACY-1215 (ricolinostat), is currently in multiple clinical trials for evaluation of its anti-tumor activity (NCT01997840, NCT02091063, NCT02632071, NCT01583283, NCT02189343, NCT02787369). Notably, it has already been shown to be safe, well-tolerated, and active in enhancing the efficacy of the combination of bortezomib and dexamethasone for multiple myeloma in a Phase II clinical trial (NCT01323751) .
In addition to the anti-tumor activity, pharmacological inhibition or genetic ablation of HDAC6 have been shown to protect against cognitive deficits in rodent models of Alzheimer’s disease (AD) [18, 20, 34]. Mechanistically, HDAC6 inhibition improves neuronal mitochondrial transport . This is interesting because synaptosomal mitochondrial damage is a key underlying mechanism of cisplatin-induced cognitive impairment . Moreover, inhibition of HDAC6 has been shown to normalize cisplatin or vincristine-induced decrease in mitochondrial transport in peripheral sensory neurons [31, 45]. Further, HDAC6 inhibition has been shown to attenuate tau pathology [13, 39, 44], a mechanism closely linked to cognitive decline in neurodegenerative diseases [19, 29, 38]. However, it is not known whether cisplatin-induced cognitive deficits are associated with tau pathology.
In the current study, we tested the hypothesis that inhibition of HDAC6 reverses CICI . We investigated the effects of the brain-penetrating HDAC6 inhibitor ACY-1083 on cisplatin-induced cognitive deficits and neuronal mitochondrial damage. We also investigated the effects of ACY-1083 on synaptic integrity, given our previous data showing reduction of dendritic spines and neuronal arborizations induced by cisplatin that is indicative of loss of synaptic function . Furthermore, we determined the effect of cisplatin and HDAC6 inhibition on tau phosphorylation.
Male C57BL/6 J mice (aged 8–10 weeks at the start of the experiment) were obtained from Jackson Laboratories (Bar Harbor, ME) and housed in The University of Texas MD Anderson Cancer Center animal facility on a reversed 12-h light/dark cycle, with free access to food and water. Animals were randomly assigned to treatment groups. All procedures were consistent with the National Institute of Health Guidelines for the Care and Use of Laboratory Animals and were approved by the Institutional Animal Care and Use Committee (IACUC) of M.D. Anderson Cancer Center. Analyses were performed by investigators blinded to treatment.
Mice were treated with cisplatin (2.3 mg/kg/day, TEVA Pharmaceuticals, North Wales, PA) diluted in sterile PBS or PBS alone intraperitoneally (i.p.) for 5 days, followed by 5 days of rest and a second round of 5 daily doses of cisplatin or PBS . The HDAC6 inhibitor ACY-1083 (Acetylon Pharmaceuticals Inc., Boston, MA) was dissolved in 20% 2-hydroxypropyl-B-cyclodextrin (Sigma-Aldrich, St. Louis, MO) + 0.5% hydroxypropyl methylcellulose (Spectrum Chemical, Gardena, CA) in water. Mice received i.p. injections of ACY-1083 at 10 mg/kg. The HDAC inhibitor ACY-1215 (Ricolinostat; Regenacy Pharmaceuticals Inc., Boston, MA) was dissolved in 10% DMSO (Sigma-Aldrich), 30% propylene glycol (Sigma-Aldrich), and 60% PEG-300 (Sigma-Aldrich), and was administered at 30 mg/kg via oral gavage .
In vivo pharmacokinetics
For in vivo pharmacokinetic studies, mice were fasted overnight and i.p. injected with 5 mg/kg ACY-1083 dissolved in 10% dimethylacetamide (DMAC, Sigma-Aldrich, St. Louis, MO) + 10% Solutol HS 15 (BASF, Houston, TX) in saline, or 30 mg/kg ACY-1215 dissolved in 10% DMAC+ 15% Solutol HS 15 in saline. Blood and brain were collected at 5 min, 15 min, 30 min, 1 h, 4 h and 8 h post injection. Plasma was obtained by centrifugation at 2000×g for 5 min at 4 °C. Brain homogenates were obtained by homogenizing the brain in 3 volumes of PBS. Plasma and brain compound level was analyzed using liquid chromatography-tandem mass spectrometry (Waters Corporation, Milford, MA) and was calculated from standard curves of ACY-1083 and ACY-1215 in mouse plasma and brain, respectively. Pharmacokinetic parameters were calculated using WinNonlin software (Certara USA, Inc., Princeton, NJ).
We used the Y-maze test , the novel object/place recognition (NOPR) test , and the puzzle box test  to assess cognitive function in mice. The tests were conducted starting 1 week after the last dose of ACY-1083 or ACY-1215 treatment. The timeline for the behavioral tests were indicated in Fig. 1a. For the Y-maze test, mice were placed in a symmetrical three-arm, gray plastic Y-maze (35 cm length × 5 cm width × 15.5 cm height per arm, with an arm angle of 120°) with external spatial room cues. Mice were placed in one of the arms, and spontaneous movement was recorded for 5 min. A perfect alternation was defined as exploration of all three arms sequentially without reentering a previously visited arm. All four paws must have been within the arm to be counted as an entrance. Alternation rate, total number of arm entries and the number of entries into each arm were recorded. The alternation rate was defined as the ratio of the number of perfect alternations to the total number of possible perfect alternations .
The NOPR test was performed as we have previously described . A testing arena (46.99 cm × 25.4 cm) was set up with two identical objects placed on the same side of the arena. Mice were placed in the testing arena for 5 min (training phase) and then placed back in their home cage. Thirty minutes later, mice were transferred back to the arena, now containing one familiar object placed at the same location as in training, and one novel object placed on the opposite end of the arena (testing phase). Investigation behavioral was defined as nose point within 1 cm of the object. The time (T) of investigative behavior toward either object during the 5-min testing phase was evaluated using EthoVision XT 10.1 video tracking software (Noldus Information Technology Inc., Leesburg, VA). Discrimination index was determined as (TNovel − TFamiliar)/(TNovel + TFamiliar).
The puzzle box test was performed 2 weeks after the last injection of ACY-1083, as previously described with slight modification . The testing area consisted of a white box divided by a black barrier into two compartments: a brightly-lit start zone (58 cm × 28 cm) and a smaller, covered goal zone (15 cm × 28 cm) connected by a 4 cm-wide underpass located under the barrier. Mice were introduced into the start zone, and the time it took for the mouse to enter the goal zone was recorded. The test consisted of 11 trials over 4 consecutive days, with two or three trials per day and obstructions of increasing difficulty placed at the underpass on day 2 to day 4. The goal zone was baited with chocolate-scented pellets (ThermoFisher Scientific, Waltham, MA). On day 1 trials 1–3, the underpass was wide open. On day 2, trial 4 was identical to the three trials on the first day. In trials 5 and 6, the underpass was filled with corncob bedding and mice had to dig their way through to get to the goal zone. On day 3, trial 7 was a repetition of trials 5 and 6. In trials 8 and 9, the underpass was obstructed with a cardboard plug that has the same color as the start zone. This task requires a series of events including spatial and short-term memory regarding the position of the underpass, recognition of the cardboard plug that’s obstructing the object, and removal of the plug for which the mice need to employ their teeth and front paws to pull out the obstruction (problem-solving), which is more complex and engage heavier cognitive load. On day 4, trials 10 and 11 were a repetition of trials 8 and 9.
Western blot analysis
Brains were homogenized in RIPA buffer (50 mM Tris·HCl, pH 7.5, 500 mM NaCl, 2.5 mM MgCl2, 1% NP-40, 10% glycerol) containing proteinase inhibitors; 30 μg of protein was separated by SDS/PAGE and transferred to PVDF membrane for immunoblot analyses. The membranes were blocked with 5% nonfat dry milk in PBS containing 0.1% Tween 20 (PBST) and probed with primary antibodies recognizing acetylated lysine (1:2000; rabbit monoclonal; Abcam (ab190479), Cambridge, UK), α-tubulin (1:1000; rabbit polyclonal; Cell Signaling Technology (2144S), Danvers, MA), and HDAC6 (1:100; goat polyclonal; Santa Cruz Biotechnology (sc-5258), Dallas, TX). Membranes were incubated with primary antibody overnight at 4 °C, washed three times with PBST, followed by incubation with horseradish peroxidase-conjugated anti-rabbit (Jackson ImmunoResearch (111–035-144), West Grove, PA), anti-goat (Jackson ImmunoResearch (111–035-144)), or anti-β-actin antibodies (Sigma-Aldrich (A3854)) at room temperature for 2 h. Membranes were then washed three times with PBST. Immunoreactivity for each protein was visualized using a chemiluminescence detection kit (GE Healthcare Life Sciences, Little Chalfont, UK). The images were acquired using ImageQuant LAS 4000 (GE Healthcare Life Sciences) and densitometrically analyzed using ImageJ software.
Synaptosome isolation and mitochondrial bioenergetics analysis
Synaptosomes were isolated as previously described . Briefly, one hemisphere of the brain was homogenized (10% w/v) into 0.32 M sucrose solution in HEPES buffer using a glass Dounce homogenizer. The lysate was centrifuged at 1000×g for 10 min at 4 °C. The supernatant was mixed with equal volume of 1.3 M sucrose in HEPES buffer and centrifuged at 20,000×g for 30 min at 4 °C. The synaptosomal pellet was then resuspended in XF media (Agilent Technologies, Santa Clara, CA) supplemented with 5.5 mM glucose, 0.5 mM sodium pyruvate, and 1 mM glutamine. Oxygen consumption rate (OCR) was measured with an XF24 Flux Analyzer (Agilent Technologies). Oligomycin (6 μM), carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP, 6 μM), and rotenone/antimycin A (2 μM each) (Sigma-Aldrich) were injected sequentially during the assay. An assay cycle of 2-min mix, 3-min wait, and 2-min measure was repeated three times for baseline rates and after each port injection. Basal respiration, ATP-linked respiration, maximal and spare respiratory capacity were determined as described previously .
Transmission electron microscopy
For transmission electron microscopy, synaptosomes were fixed in 2% glutaraldehyde + 2% paraformaldehyde in PBS for over 24 h. Samples were then processed as previously described . The samples were polymerized in a 60 °C oven for approximately 3 days. Ultrathin sections were cut in a Leica Ultracut microtome (Leica Microsystems, Wetzlar, Germany), stained with uranyl acetate and lead citrate in a Leica EM Stainer (Leica Microsystems), and examined in a JEM 1010 transmission electron microscope (JEOL USA, Inc., Peabody, MA) at an accelerating voltage of 80 kV. Digital images were obtained using AMT Imaging System (Advanced Microscopy Techniques Corp, Danvers, MA). 25–35 mitochondria were quantified from each group (4 mice/group, with 5 images/mouse). Atypical mitochondria were identified by 2-fold increases in diameter and/or excessive vacuolization (more than 50% translucent). The percentage of atypical mitochondria was calculated for each group.
Mice were euthanized by CO2 euthanasia and perfused intracardially with ice-cold PBS. The skin and skull on top of the brain were removed to allow access to the brain. Brains were removed subsequently and transferred to 4% paraformaldehyde. Brains were post-fixed in 4% paraformaldehyde for 24 h, cryoprotected in sucrose, and frozen in optimal cutting temperature compound (Sakura Finetek, Torrance, CA). Coronal brain sections (8 μm) were blocked with blocking buffer (10% normal goat serum, 2% BSA and 0.1% saponin in PBS), followed by incubation with rabbit anti-synaptophysin (1:1000, MilliporeSigma (AB9272), Burlington, MA), rabbit anti-PSD95 (1:1000; Abcam (ab18258)), or mouse anti-phospho-tau (Ser202, Thr205) (AT8) (1:100; ThermoFisher Scientific (MN1020)) diluted in antibody buffer (2% normal goat serum, 2% BSA and 0.1% saponin in PBS) at 4 °C overnight. Slides were then washed three times with PBS, followed by incubation with Alexa-488 goat anti-rabbit (1:1000; Invitrogen (A-21206), Carlsbad, CA) for synaptophysin, Alexa-594 goat anti-rabbit (1:500; Invitrogen (A11037)) for PSD95, and Alexa-488 goat anti-mouse (1:500; Invitrogen (A-21202)) for phospho-tau at room temperature for 2 h. For negative control sections, primary antibody was omitted. After antibody staining, slides were washed three times with PBS, followed by incubation with DAPI (1,5000; Sigma-Aldrich) for 5 min. Slides were then washed three times with PBS, and sealed with FluorSave Reagent (MilliporeSigma). Sections were visualized using a Leica fluorescence microscope (Leica Microsystems). The mean intensity of fluorescence was quantified in three sections (60 μm apart) per mouse using ImageJ software.
Statistical analyses were performed using GraphPad Prism version 7.01 (GraphPad, San Diego, CA). Error bars indicate SEM and statistical significance was assessed by two-way ANOVA with Tukey’s post-hoc analysis. Experimental replicates labeled as “n” were derived from at least four animals as indicated and are described further in each results section. Significance is reported in the results section and full statistical results are detailed in the results section, figure legends and Additional file 1: Table S1.
The HDAC6 inhibitor ACY-1083 reverses cisplatin-induced cognitive impairment
Cisplatin (2.3 mg/kg) or PBS was administered intraperitoneally every day for 5 days, followed by 5 days of rest and a second cycle of 5 daily injections. This dosing schedule is commonly used to study neurotoxic effects of cisplatin in mice [11, 31, 33, 35]. The cumulative dose of cisplatin has been shown to effectively control tumor growth in mice . Treatment with the HDAC6 inhibitor ACY-1083 was started 3 days after the last dose of cisplatin and continued for 2 weeks (Fig. 1a). Previous studies of our lab have demonstrated that the cognitive deficits in response to cisplatin are present in both male and female mice with no sex difference observed. For the HDAC6 inhibitors, we have used both male and female mice for our cisplatin-induced peripheral neuropathy study and also did not find any sex difference. Therefore, we used only male mice in the current study.
Pharmacokinetic analysis of ACY-1083
ACY-1083 plasma concentration
ACY-1083 Brain concentration
Brain to plasma ration
Pharmacokinetic analysis of ACY-1215
ACY-1215 plasma concentration
ACY-1215 Brain concentration
Brain to plasma ration
The puzzle box test has been established as a problem-solving task to study executive functioning in mice . During the test, mice were introduced into the brightly lit start zone, and the time to enter the dark goal box via a connecting underpass was recorded. There was no effect of cisplatin during trial 1–4 when the underpass was unobstructed, or trial 5–7 when the underpass was filled with bedding. However, cisplatin-treated mice used significantly more time than the PBS-treated mice when the difficult obstacle (cardboard plug, trials 8–11) was covering the underpass (post-hoc test, p < 0.0001) (Fig. 2c). Notably, 2 weeks of ACY-1083 treatment completely restored the executive functioning of cisplatin-treated mice (post-hoc test, p < 0.0001). ACY-1083 alone did not have any effect in any of the behavioral tests.
Taken together, these findings indicate that cisplatin impaired spatial working memory, executive functioning, and problem-solving abilities in mice and, more importantly, that the brain-penetrating HDAC6 inhibitor ACY-1083 reversed these deficits.
ACY-1083 modulates α-tubulin acetylation in the brain
ACY-1083 reverses cisplatin-induced impairment in synaptosomal mitochondrial morphology and bioenergetics
Furthermore, cisplatin induced significant decreases in parameters measuring synaptosomal mitochondrial bioenergetics, including the maximal respiratory capacity (MRC) (post-hoc test, p = 0.0416) (Fig. 4f) and spare respiratory capacity (SRC) (post-hoc test, p = 0.0147) (Fig. 4g). ACY-1083 treatment also restored cisplatin-induced impairment in MRC (post-hoc test, p = 0.0345) and SRC (post-hoc test, p = 0.0291) (Fig. 4f and g). Cisplatin and ACY-1083 did not affect basal respiration (two-way ANOVA, F (1, 20) = 0.6219, p = 0.4396) (Fig. 4h) or ATP-coupled respiration (two-way ANOVA, F (1, 20) = 0.02046, p = 0.8877; n = 6) (Fig. 4i).
ACY-1083 restores expression of markers of synaptic integrity in cisplatin-treated mice
ACY-1083 reverses tau pathology in cisplatin-treated mice
We show here for the first time that pharmacological inhibition of HDAC6 with the brain-penetrating inhibitor ACY-1083 reverses cisplatin-induced cognitive impairment as assessed in multiple behavioral tests. We have previously demonstrated that the same regimen of HDAC6 inhibition resolves cisplatin-induced neuropathy . Moreover, HDAC6 inhibitors have been shown to enhance tumor control in Phase I and II clinical trials . Therefore, inhibition of HDAC6 appears as a promising therapeutic strategy for reversing neurotoxic side effects of chemotherapy while improving the efficacy of cancer treatment.
Our result suggests that penetration of the inhibitor into the brain is likely required, as the non-brain-penetrating HDAC6 inhibitor ACY-1215 had no effect on cognitive function at a dosing schedule that reversed cisplatin-induced peripheral neuropathy . The reversal of cisplatin-induced cognitive impairment by the brain-penetrating inhibitor ACY-1083 was associated with reversal of synaptosomal mitochondrial deficits and restoration of synaptic integrity. Furthermore, we demonstrate that deacetylation of α-tubulin and hyperphosphorylation of tau were part of the mechanism contributing to cisplatin-induced cognitive impairment, and that HDAC6 inhibition reversed these pathological changes as well.
Cognitive impairment in neurodegenerative diseases is frequently accompanied by disruption of microtubule stability [4, 41]. However, no such findings have ever been reported in CICI. To our knowledge, we are the first to demonstrate that cisplatin-induced cognitive impairment is associated with deacetylation of the microtubule protein α-tubulin and hyperphosphorylation of the microtubule-associated protein tau, which are indicative of changes in microtubule stability. Our data indicate that cisplatin induced a significant reduction in α-tubulin acetylation in the brain. We propose that this could be attributed to increased HDAC6 activity, as no change in HDAC6 protein level was detected. However, downregulation or inhibition of α-tubulin acetyltransferases, such as αTAT1, could also be involved in cisplatin-induced α-tubulin deacetylation . Inhibition of HDAC6 normalized the level of acetylated α-tubulin in the brain. ACY-1083 alone did not have any effect. This is consistent with a previous report showing no detectable changes in α-tubulin acetylation in brains of HDAC6 knockout mice, which was attributed to high baseline level of α-tubulin acetylation in the brain .
The microtubule-associated protein tau plays an essential role in the modulation of microtubule assembly and stability and the maintenance of normal neuronal morphology. Dysregulation of tau, including tau hyperphosphorylation and formation of neurofilament tangles, has been reported in various experimental models of aging and neurodegenerative diseases, as well as in AD patients . Recently, tau pathology has also been linked to cognitive disorders that develop in response to head trauma, where perivascular accumulation of abnormally phosphorylated tau was detected in the depths of cortical sulci . We show here that cisplatin induced tau hyperphosphorylation in the DG and CA1 regions of the hippocampus, a phenomenon that correlate with onset of cognitive decline in AD and other tauopathy models [19, 38], and that ACY-1083 treatment reversed tau hyperphosphorylation.
Hyperphosphorylated tau displays reduced binding to microtubules, leading to disrupted microtubule assembly, hyperdynamic microtubules, and decreased axonal transport of cellular organelles, including the mitochondria . More specifically, pathological phosphorylation of tau has been shown to inhibit kinesin-1 motility, thereby limiting kinesin-1-dependent axonal mitochondrial transport . In contrast, increases in α-tubulin acetylation can enhance mitochondrial movement through increased recruitment of kinesin-1 to microtubules . In our cisplatin model of CICI, we observed morphologically distorted and functionally disrupted mitochondria in the synaptosomes. Importantly, these cisplatin-induced synaptosomal mitochondrial deficits were reversed by the HDAC6 inhibitor ACY-1083, along with restoration of α-tubulin acetylation and reversal of tau hyperphosphorylation. We have found evidence for disruption of mitochondrial trafficking by cisplatin in sensory neurons, which was rescued by selective inhibition of HDAC6 . Therefore, we propose the normalization of α-tubulin acetylation and tau phosphorylation as mechanism of action for HDAC6 inhibitors, which allows stabilization of microtubules and restoration of microtubule-dependent axonal transport of mitochondria, thereby restoring synaptosomal mitochondrial function. In this respect, data showing that pharmacological or genetic disruption of HDAC6 is protective against pathological tau-induced microtubule abnormality and cognitive decline are of great interest [9, 13, 20, 34, 39, 50]. For example, Xiong et al. demonstrated that HDAC6 null mutation or HDAC6 inhibition rescued tau-induced microtubule defects and neuromuscular junction abnormalities in drosophila by enhancing microtubule acetylation . The HDAC6 inhibitor tubastatin has been shown to reduce the level of total tau and restore memory function in the rTg4510 mouse model of AD . Taken together, we propose that HDAC6 inhibition restores cognitive function in cisplatin-treated mice through reversal of tau phosphorylation and normalization of α-tubulin acetylation, leading to restoration of synaptosomal mitochondrial function.
Synaptic dysfunction and loss of synaptic integrity are among the early signs of neurodegeneration . In our previous studies, we have shown that cisplatin decreased dendritic spine density and dendritic arborization in the cingulate cortex , which reflects alterations in the functionality of resident synapses . Following this line of thought, we observed here disrupted synaptic integrity in response to cisplatin, as evidenced by decreases in presynaptic and postsynaptic markers in the hippocampus. Moreover, we show that ACY-1083 restored synaptic integrity and that this corresponded with reversal of behavioral deficits. In neurons, synapses represent sites of high energy demand and extensive Ca2+ fluctuation, where large amounts of mitochondria are needed to maintain proper synaptic function and integrity . These features render synapses extremely susceptible to perturbations in mitochondrial function and trafficking . Pathological tau has also been linked to synaptotoxicity . Intracellular accumulation of tau induces synaptic degeneration and memory deficits . In AD brains, the presence of hyperphosphorylated tau highly correlates with reduction in presynaptic protein expression . Pathogenic tau binds to synaptic vesicles and interferes with presynaptic functions by lowering synaptic vesicle mobility and release rate . Given the protective effects of ACY-1083 on synaptosomal mitochondrial function and tau pathology, we propose that HDAC6 inhibition restores synaptic integrity at least partially through normalization of mitochondrial transport and function, and reversal of tau hyperphosphorylation.
In the current study, we show that cisplatin induced a significant decrease in the acetylation of α-tubulin without affecting the expression level of HDAC6, which suggests that cisplatin may enhance the deacetylase activity of HDAC6. Previous studies have implicated tau as a deacetylation target of HDAC6 [9, 43, 44]. Therefore, we examined tau acetylation with the only commercially available antibody that recognizes tau acetylation at the lysine residue K280, a previously identified target site for HDAC6 [43, 44]. However, we did not detect any change associated with tau acetylation at K280 with either cisplatin or ACY-1083 (data not shown). We cannot rule out the possibility that cisplatin and/or ACY-1083 modulate tau phosphorylation through regulation of acetylation on other lysine residues, such as the KXGS motifs within the microtubule-binding domain. Of note, tau hypoacetylation on KXGS motifs has been associated with tau hyperphosphorylation and cognitive deficits in a mouse model of tauopathy and in AD patients . Furthermore, the tau hyperphosphorylation phenotype observed in mice with tauopathy can be reversed by HDAC6 inhibition-mediated tau acetylation . Therefore, the tau pathology observed in our cisplatin model might be attributed to upregulated HDAC6 activity leading to hypoacetylation of tau on certain residues, which was reversed by ACY-1083. The deacetylase activity of HDAC6 can be regulated by its posttranslational modification status [10, 32, 48]. For example, upregulated deacetylase activity of HDAC6 has been associated with its phosphorylation by GSK3β, GRK2, and CK2 [10, 32, 48]. Interestingly, activation of some of these kinases, such as GSK3β and CK2, are implicated in neurodegeneration and tau hyperphosphorylation . Therefore, it is possible that activation of one or more of these protein kinases underlies cisplatin-induced tau hyperphosphorylation, HDAC6 activation and α-tubulin deacetylation.
In summary, our results provide evidence that the brain-penetrating HDAC6 inhibitor ACY-1083 is a promising therapeutic candidate for the treatment of CICI. Our findings indicate that dysregulation of microtubule dynamics, including deacetylation of α-tubulin and hyperphosphorylation of tau, contribute to the pathogenesis of CICI. Inhibition of HDAC6 reverses cisplatin-induced α-tubulin deacetylation and tau hyperphosphorylation and restores synaptosomal mitochondrial function, which likely contribute to the restoration of synaptic integrity. Importantly, HDAC6 inhibitors have been shown to be safe and active as add-on cancer therapy . Therefore, they hold great promises as a class of drugs that could reverse chemotherapy-induced neurotoxicity without negatively affecting cancer treatment.
The authors acknowledge Jeanie F. Woodruff, BS, ELS, for editorial assistance.
The electron microscopy work was done with technical support from MD Anderson’s High Resolution Electron Microscopy Facility, supported by MD Anderson Cancer Center Support Grant NIH P30 CA016672 (PI: Pisters).
This study is supported by funding from Regenacy Pharmaceuticals Inc. and R01 grants CA208371 (PI: Heijnen) and CA227064 (PI: Kavelaars) from the National Institutes of Health (NIH).
Availability of data and materials
All data generated or analysed during this study are included in this published article [and its supplementary information files].
JM, CJH, and AK designed the research; JM and XH conducted experiments; JM, XH and MBJ analyzed the data; JM, CJH, and AK wrote the manuscript; all authors edited the manuscript and approved the final version.
Ethics approval and consent to participate
All animal work procedures were consistent with the National Institute of Health Guidelines for the Care and Use of Laboratory Animals and were approved by the Institutional Animal Care and Use Committee (IACUC) of M.D. Anderson Cancer Center.
Consent for publication
M.J. is employed by Regenacy Pharmaceuticals Inc. This study was supported in part by a research grant from Regenacy Pharmaceuticals to A.K. And C.J.H.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Aldana-Masangkay GI, Sakamoto KM (2011) The role of HDAC6 in cancer. J Biomed Biotechnol 2011:875824. https://doi.org/10.1155/2011/875824 View ArticleGoogle Scholar
- Amengual JE, Johannet P, Lombardo M, Zullo K, Hoehn D, Bhagat G, Scotto L, Jirau-Serrano X, Radeski D, al HJ (2015) Dual targeting of protein degradation pathways with the selective HDAC6 inhibitor ACY-1215 and Bortezomib is synergistic in lymphoma. Clin Cancer res 21:4663–4675. https://doi.org/10.1158/1078-0432.CCR-14-3068 View ArticlePubMed CentralPubMedGoogle Scholar
- Antunes M, Biala G (2012) The novel object recognition memory: neurobiology, test procedure, and its modifications. Cogn Process 13:93–110. https://doi.org/10.1007/s10339-011-0430-z View ArticleGoogle Scholar
- Barten DM, Fanara P, Andorfer C, Hoque N, Wong PY, Husted KH, Cadelina GW, Decarr LB, Yang L, Liu V et al (2012) Hyperdynamic microtubules, cognitive deficits, and pathology are improved in tau transgenic mice with low doses of the microtubule-stabilizing agent BMS-241027. J Neurosci 32:7137–7145. https://doi.org/10.1523/JNEUROSCI.0188-12.2012 View ArticleGoogle Scholar
- Ben Abdallah NM, Fuss J, Trusel M, Galsworthy MJ, Bobsin K, Colacicco G, Deacon RM, Riva MA, Kellendonk C, Sprengel R et al (2011) The puzzle box as a simple and efficient behavioral test for exploring impairments of general cognition and executive functions in mouse models of schizophrenia. Exp Neurol 227:42–52. https://doi.org/10.1016/j.expneurol.2010.09.008 View ArticleGoogle Scholar
- Bishop NA, Lu T, Yankner BA (2010) Neural mechanisms of ageing and cognitive decline. Nature 464:529–535. https://doi.org/10.1038/nature08983 View ArticlePubMed CentralPubMedGoogle Scholar
- Bourne JN, Harris KM (2008) Balancing structure and function at hippocampal dendritic spines. Annu Rev Neurosci 31:47–67. https://doi.org/10.1146/annurev.neuro.31.060407.125646 View ArticlePubMed CentralPubMedGoogle Scholar
- Cai Q, Tammineni P (2017) Mitochondrial aspects of synaptic dysfunction in Alzheimer's disease. J Alzheimers Dis 57:1087–1103. https://doi.org/10.3233/JAD-160726 View ArticlePubMed CentralPubMedGoogle Scholar
- Carlomagno Y, Chung DC, Yue M, Castanedes-Casey M, Madden BJ, Dunmore J, Tong J, DeTure M, Dickson DW, Petrucelli L et al (2017) An acetylation-phosphorylation switch that regulates tau aggregation propensity and function. J Biol Chem 292:15277–15286. https://doi.org/10.1074/jbc.M117.794602 View ArticlePubMed CentralPubMedGoogle Scholar
- Chen S, Owens GC, Makarenkova H, Edelman DB (2010) HDAC6 regulates mitochondrial transport in hippocampal neurons. PLoS One 5:e10848. https://doi.org/10.1371/journal.pone.0010848 View ArticlePubMed CentralPubMedGoogle Scholar
- Chiu GS, Maj MA, Rizvi S, Dantzer R, Vichaya EG, Laumet G, Kavelaars A, Heijnen CJ (2017) Pifithrin-mu prevents cisplatin-induced Chemobrain by preserving neuronal mitochondrial function. Cancer Res 77:742–752. https://doi.org/10.1158/0008-5472.CAN-16-1817 View ArticleGoogle Scholar
- Coleman PD, Yao PJ (2003) Synaptic slaughter in Alzheimer's disease. Neurobiol Aging 24:1023–1027View ArticleGoogle Scholar
- Cook C, Carlomagno Y, Gendron TF, Dunmore J, Scheffel K, Stetler C, Davis M, Dickson D, Jarpe M, Deture M et al (2014) Acetylation of the KXGS motifs in tau is a critical determinant in modulation of tau aggregation and clearance. Hum Mol Genet 23:104–116. https://doi.org/10.1093/hmg/ddt402 View ArticleGoogle Scholar
- de Moor JS, Mariotto AB, Parry C, Alfano CM, Padgett L, Kent EE, Forsythe L, Scoppa S, Hachey M, Rowland JH (2013) Cancer survivors in the United States: prevalence across the survivorship trajectory and implications for care. Cancer Epidemiol Biomark Prev 22:561–570. https://doi.org/10.1158/1055-9965.EPI-12-1356 View ArticleGoogle Scholar
- Deacon RM, Rawlins JN (2006) T-maze alternation in the rodent. Nat Protoc 1:7–12. https://doi.org/10.1038/nprot.2006.2 View ArticleGoogle Scholar
- Deprez S, Amant F, Smeets A, Peeters R, Leemans A, Van Hecke W, Verhoeven JS, Christiaens MR, Vandenberghe J, Vandenbulcke M et al (2012) Longitudinal assessment of chemotherapy-induced structural changes in cerebral white matter and its correlation with impaired cognitive functioning. J Clin Oncol 30:274–281. https://doi.org/10.1200/JCO.2011.36.8571 View ArticleGoogle Scholar
- Dietrich J, Monje M, Wefel J, Meyers C (2008) Clinical patterns and biological correlates of cognitive dysfunction associated with cancer therapy. Oncologist 13:1285–1295. https://doi.org/10.1634/theoncologist.2008-0130 View ArticleGoogle Scholar
- Dompierre JP, Godin JD, Charrin BC, Cordelieres FP, King SJ, Humbert S, Saudou F (2007) Histone deacetylase 6 inhibition compensates for the transport deficit in Huntington's disease by increasing tubulin acetylation. J Neurosci 27:3571–3583. https://doi.org/10.1523/JNEUROSCI.0037-07.2007 View ArticleGoogle Scholar
- Gotz J, Gladbach A, Pennanen L, van Eersel J, Schild A, David D, Ittner LM (2010) Animal models reveal role for tau phosphorylation in human disease. Biochim Biophys Acta 1802: 860–871 Doi https://doi.org/10.1016/j.bbadis.2009.09.008 View ArticleGoogle Scholar
- Govindarajan N, Rao P, Burkhardt S, Sananbenesi F, Schluter OM, Bradke F, Lu J, Fischer A (2013) Reducing HDAC6 ameliorates cognitive deficits in a mouse model for Alzheimer's disease. EMBO Mol Med 5:52–63. https://doi.org/10.1002/emmm.201201923 View ArticleGoogle Scholar
- Grozinger CM, Hassig CA, Schreiber SL (1999) Three proteins define a class of human histone deacetylases related to yeast Hda1p. Proc Natl Acad Sci U S A 96:4868–4873View ArticlePubMedGoogle Scholar
- Henley SJ, Singh SD, King J, Wilson RJ, O'Neil ME, Ryerson AB (2017) Invasive Cancer incidence and survival - United States, 2013. MMWR Morb mortal Wkly rep 66: 69–75 Doi https://doi.org/10.15585/mmwr.mm6603a1 View ArticlePubMedGoogle Scholar
- Hsiao KK, Borchelt DR, Olson K, Johannsdottir R, Kitt C, Yunis W, Xu S, Eckman C, Younkin S, al PD (1995) Age-related CNS disorder and early death in transgenic FVB/N mice overexpressing Alzheimer amyloid precursor proteins. Neuron 15:1203–1218View ArticleGoogle Scholar
- Huang P, Almeciga-Pinto I, Jarpe M, van Duzer JH, Mazitschek R, Yang M, Jones SS, Quayle SN (2017) Selective HDAC inhibition by ACY-241 enhances the activity of paclitaxel in solid tumor models. Oncotarget 8: 2694-2707 Doi https://doi.org/10.18632/oncotarget.13738
- Hubbert C, Guardiola A, Shao R, Kawaguchi Y, Ito A, Nixon A, Yoshida M, Wang XF, Yao TP (2002) HDAC6 is a microtubule-associated deacetylase. Nature 417:455–458. https://doi.org/10.1038/417455a View ArticleGoogle Scholar
- Janelsins MC, Kesler SR, Ahles TA, Morrow GR (2014) Prevalence, mechanisms, and management of cancer-related cognitive impairment. Int Rev Psychiatry 26:102–113. https://doi.org/10.3109/09540261.2013.864260 View ArticlePubMed CentralPubMedGoogle Scholar
- Kalebic N, Sorrentino S, Perlas E, Bolasco G, Martinez C, Heppenstall PA (2013) alphaTAT1 is the major alpha-tubulin acetyltransferase in mice. Nat Commun 4: 1962. https://doi.org/10.1038/ncomms2962
- Kesler SR, Blayney DW (2016) Neurotoxic effects of anthracycline- vs nonanthracycline-based chemotherapy on cognition in breast Cancer survivors. JAMA Oncol 2:185–192. https://doi.org/10.1001/jamaoncol.2015.4333 View ArticlePubMed CentralPubMedGoogle Scholar
- Kovacs GG (2015) Invited review: neuropathology of tauopathies: principles and practice. Neuropathol Appl Neurobiol 41:3–23. https://doi.org/10.1111/nan.12208 View ArticleGoogle Scholar
- Kovacs JJ, Murphy PJ, Gaillard S, Zhao X, Wu JT, Nicchitta CV, Yoshida M, Toft DO, Pratt WB, Yao TP (2005) HDAC6 regulates Hsp90 acetylation and chaperone-dependent activation of glucocorticoid receptor. Mol Cell 18:601–607. https://doi.org/10.1016/j.molcel.2005.04.021 View ArticleGoogle Scholar
- Krukowski K, Ma J, Golonzhka O, Laumet GO, Gutti T, van Duzer JH, Mazitschek R, Jarpe MB, Heijnen CJ, Kavelaars A (2017) HDAC6 inhibition effectively reverses chemotherapy-induced peripheral neuropathy. Pain: Doi https://doi.org/10.1097/j.pain.0000000000000893 View ArticlePubMedGoogle Scholar
- Lafarga V, Aymerich I, Tapia O, Mayor F Jr, Penela P (2012) A novel GRK2/HDAC6 interaction modulates cell spreading and motility. EMBO J 31:856–869. https://doi.org/10.1038/emboj.2011.466 View ArticleGoogle Scholar
- Maj MA, Ma J, Krukowski KN, Kavelaars A, Heijnen CJ (2017) Inhibition of mitochondrial p53 accumulation by PFT-mu prevents cisplatin-induced peripheral neuropathy. Front Mol Neurosci 10:108. https://doi.org/10.3389/fnmol.2017.00108 View ArticlePubMed CentralPubMedGoogle Scholar
- Majid TG D.; Criss II Z, Jarpe M.; Pautler R.G. (2015) Pharmocologic treatment with histone deacetylase 6 inhibitor (ACY-738) recovers Alzheimer's disease phenotype in amyloid precursor protein/presenilin 1 (APP/PS1) mice. Alzheimer's & dementia: Translational Research & Clinical Interventions 1: 170-181 Doi https://doi.org/10.1016/j.trci.2015.08.001 Google Scholar
- Mao-Ying QL, Kavelaars A, Krukowski K, Huo XJ, Zhou W, Price TJ, Cleeland C, Heijnen CJ (2014) The anti-diabetic drug metformin protects against chemotherapy-induced peripheral neuropathy in a mouse model. PLoS One 9:e100701. https://doi.org/10.1371/journal.pone.0100701 View ArticlePubMed CentralPubMedGoogle Scholar
- McDonald BC, Conroy SK, Ahles TA, West JD, Saykin AJ (2012) Alterations in brain activation during working memory processing associated with breast cancer and treatment: a prospective functional magnetic resonance imaging study. J Clin Oncol 30:2500–2508. https://doi.org/10.1200/JCO.2011.38.5674 View ArticlePubMed CentralPubMedGoogle Scholar
- Pooler AM, Noble W, Hanger DP (2014) A role for tau at the synapse in Alzheimer's disease pathogenesis. Neuropharmacology 76 Pt a: 1-8. https://doi.org/10.1016/j.neuropharm.2013.09.018 View ArticleGoogle Scholar
- Ramsden M, Kotilinek L, Forster C, Paulson J, McGowan E, SantaCruz K, Guimaraes A, Yue M, Lewis J, Carlson Get al (2005) Age-dependent neurofibrillary tangle formation, neuron loss, and memory impairment in a mouse model of human tauopathy (P301L). J Neurosci 25: 10637–10647 Doi https://doi.org/10.1523/JNEUROSCI.3279-05.2005 View ArticleGoogle Scholar
- Selenica ML, Benner L, Housley SB, Manchec B, Lee DC, Nash KR, Kalin J, Bergman JA, Kozikowski A, Gordon MNet al (2014) Histone deacetylase 6 inhibition improves memory and reduces total tau levels in a mouse model of tau deposition. Alzheimers Res Ther 6: 12 Doi https://doi.org/10.1186/alzrt241 View ArticleGoogle Scholar
- Sheng ZH (2014) Mitochondrial trafficking and anchoring in neurons: new insight and implications. J Cell Biol 204:1087–1098. https://doi.org/10.1083/jcb.201312123 View ArticlePubMed CentralPubMedGoogle Scholar
- Stern JL, Lessard DV, Hoeprich GJ, Morfini GA, Berger CL (2017) Phosphoregulation of tau modulates inhibition of kinesin-1 motility. Mol Biol Cell 28:1079–1087. https://doi.org/10.1091/mbc.E16-10-0728 View ArticlePubMed CentralPubMedGoogle Scholar
- Tagge CA, Fisher AM, Minaeva OV, Gaudreau-Balderrama A, Moncaster JA, Zhang XL, Wojnarowicz MW, Casey N, Lu H, Kokiko-Cochran ONet al (2018) Concussion, microvascular injury, and early tauopathy in young athletes after impact head injury and an impact concussion mouse model. Brain 141: 422–458 Doi https://doi.org/10.1093/brain/awx350 View ArticlePubMedGoogle Scholar
- Trzeciakiewicz H, Tseng JH, Wander CM, Madden V, Tripathy A, Yuan CX, Cohen TJ (2017) A dual pathogenic mechanism links tau acetylation to sporadic Tauopathy. Sci Rep 7:44102. https://doi.org/10.1038/srep44102 View ArticlePubMed CentralPubMedGoogle Scholar
- Tseng JH, Xie L, Song S, Xie Y, Allen L, Ajit D, Hong JS, Chen X, Meeker RB, Cohen TJ (2017) The deacetylase HDAC6 mediates endogenous Neuritic tau pathology. Cell Rep 20:2169–2183. https://doi.org/10.1016/j.celrep.2017.07.082 View ArticlePubMed CentralPubMedGoogle Scholar
- Van Helleputte L, Kater M, Cook DP, Eykens C, Rossaert E, Haeck W, Jaspers T, Geens N, Vanden Berghe P, Gysemans Cet al (2018) Inhibition of histone deacetylase 6 (HDAC6) protects against vincristine-induced peripheral neuropathies and inhibits tumor growth. Neurobiol Dis 111: 59–69 Doi https://doi.org/10.1016/j.nbd.2017.11.011 View ArticleGoogle Scholar
- Vogl DT, Raje N, Jagannath S, Richardson P, Hari P, Orlowski R, Supko JG, Tamang D, Yang M, Jones SSet al (2017) Ricolinostat, the first selective histone deacetylase 6 inhibitor, in combination with Bortezomib and dexamethasone for relapsed or refractory multiple myeloma. Clin Cancer res 23: 3307–3315 Doi https://doi.org/10.1158/1078-0432.CCR-16-2526 View ArticlePubMedGoogle Scholar
- Wang Y, Chen L, Huang G, He D, He J, Xu W, Zou C, Zong F, Li Y, Chen Bet al (2013) Klotho sensitizes human lung cancer cell line to cisplatin via PI3k/Akt pathway. PLoS One 8: e57391 Doi https://doi.org/10.1371/journal.pone.0057391 View ArticlePubMedGoogle Scholar
- Watabe M, Nakaki T (2011) Protein kinase CK2 regulates the formation and clearance of aggresomes in response to stress. J Cell Sci 124:1519–1532. https://doi.org/10.1242/jcs.081778 View ArticleGoogle Scholar
- Wefel JS, Schagen SB (2012) Chemotherapy-related cognitive dysfunction. Curr Neurol Neurosci Rep 12:267–275. https://doi.org/10.1007/s11910-012-0264-9 View ArticleGoogle Scholar
- Xiong Y, Zhao K, Wu J, Xu Z, Jin S, Zhang YQ (2013) HDAC6 mutations rescue human tau-induced microtubule defects in Drosophila. Proc Natl Acad Sci U S A 110:4604–4609. https://doi.org/10.1073/pnas.1207586110 View ArticlePubMed CentralPubMedGoogle Scholar
- Yin Y, Gao D, Wang Y, Wang ZH, Wang X, Ye J, Wu D, Fang L, Pi G, Yang Yet al (2016) Tau accumulation induces synaptic impairment and memory deficit by calcineurin-mediated inactivation of nuclear CaMKIV/CREB signaling. Proc Natl Acad Sci U S A 113: E3773–E3781 Doi https://doi.org/10.1073/pnas.1604519113 View ArticleGoogle Scholar
- Zhang Y, Kwon S, Yamaguchi T, Cubizolles F, Rousseaux S, Kneissel M, Cao C, Li N, Cheng HL, Chua Ket al (2008) Mice lacking histone deacetylase 6 have hyperacetylated tubulin but are viable and develop normally. Mol Cell Biol 28: 1688–1701 Doi https://doi.org/10.1128/MCB.01154-06 View ArticlePubMedGoogle Scholar
- Zhou L, McInnes J, Wierda K, Holt M, Herrmann AG, Jackson RJ, Wang YC, Swerts J, Beyens J, Miskiewicz K et al (2017) Tau association with synaptic vesicles causes presynaptic dysfunction. Nat Commun 8:15295. https://doi.org/10.1038/ncomms15295 View ArticlePubMed CentralPubMedGoogle Scholar
- Zhou W, Kavelaars A, Heijnen CJ (2016) Metformin prevents cisplatin-induced cognitive impairment and brain damage in mice. PLoS One 11:e0151890. https://doi.org/10.1371/journal.pone.0151890 View ArticlePubMed CentralPubMedGoogle Scholar