Fig. 1

Effect of HDAC6 inhibition on cisplatin-induced cognitive impairment in the Y maze test. a Mice were treated with two 5-day cycles of cisplatin or PBS, followed by 14 daily administrations of HDAC6 inhibitor (either ACY-1083 or ACY-1215) or vehicle starting 3 days after the last dose of cisplatin/PBS. Behavioral tests including Y-maze, NOPR, and the puzzle box tests were started one week post the last ACY-1083 injection as indicated in the timeline. The Y-maze test of spontaneous alternations was performed 1 week after the last injection of the two HDAC6 inhibitors. The percentage of perfect alternations (alternation rate) was calculated: (b) ACY-1083 (n = 8; two-way ANOVA with Tukey’s post-hoc analysis: F (1, 28) = 5.547, p = 0.0258; PBS vs. Cisplatin, p = 0.0086; Cisplatin vs. Cisplatin + ACY-1083, p = 0.0074) or (c) ACY-1215 (n = 6; two-way ANOVA with Tukey’s post-hoc analysis: F (1, 20) = 0.3809, p = 0.5441; PBS vs. Cisplatin, p = 0.0148; PBS vs. Cisplatin + ACY-1215, p = 0.0096). Total number of arm entries and number of arm entries into each individual arm: (d) ACY-1083 (n = 8; two-way ANOVA, F (1, 28) = 0.002633, p = 0.9594) (e) ACY-1215 (n = 6; two-way ANOVA, F (1, 20) = 0.7648, p = 0.3922). Results are expressed as means ± SEM; *p < 0.05, **p < 0.01