Dorsal root ganglia in Friedreich ataxia: satellite cell proliferation and inflammation
© Koeppen et al. 2016
Received: 10 February 2016
Accepted: 13 February 2016
Published: 3 May 2016
Dorsal root ganglia (DRG) are highly vulnerable to frataxin deficiency in Friedreich ataxia (FA), an autosomal recessive disease due to pathogenic homozygous guanine-adenine-adenine trinucleotide repeat expansions in intron 1 of the FXN gene (chromosome 9q21.11). An immunohistochemical and immunofluorescence study of DRG in 15 FA cases and 12 controls revealed that FA causes major primary changes in satellite cells and inflammatory destruction of neurons. A panel of antibodies was used to reveal the cytoplasm of satellite cells (glutamine synthetase, S100, metabotropic glutamate receptors 2/3, excitatory amino acid transporter 1, ATP-sensitive inward rectifier potassium channel 10, and cytosolic ferritin), gap junctions (connexin 43), basement membranes (laminin), mitochondria (ATP synthase subunit beta and frataxin), and monocytes (CD68 and IBA1).
Reaction product of the cytoplasmic markers and laminin confirmed proliferation of satellite cells and processes into multiple perineuronal layers and residual nodules. The formation of connexin 43-reactive gap junctions between satellite cells was strongly upregulated. Proliferating satellite cells in FA displayed many more frataxin- and ATP5B-reactive mitochondria than normal. Monocytes entered into the satellite cell layer, appeared to penetrate neuronal plasma membranes, and infiltrated residual nodules. Satellite cells and IBA1-reactive monocytes displayed upregulated ferritin biosynthesis, which was most likely due to leakage of iron from dying neurons.
We conclude that FA differentially affects the key cellular elements of DRG, and postulate that the disease causes loss of bidirectional trophic support between satellite cells and neurons.
Friedreich ataxia (FA) is an autosomal recessive disease with a prevalence in peoples of Western European (Caucasian) origin ranging from 1:43,000 to 1:140,000 . The underlying mutation in the vast majority of cases is a homozygous guanine-adenine-adenine (GAA) trinucleotide repeat expansion in intron 1 of the FXN gene on chromosome 9q21.11. While the mutation causes a systemic deficiency of frataxin, a small mitochondrial protein, the clinical and neuropathological phenotypes are very diverse. In the central and peripheral nervous systems, FA affects motor cortex (Betz cells), dentate nucleus, spinal cord (dorsal nuclei in Clarke columns, dorsal columns, and dorsal spinocerebellar and corticospinal tracts), dorsal root ganglia (DRG), and sensory peripheral nerves [11, 12]. FA also causes hypertrophic cardiomyopathy, diabetes mellitus due to β-cell atrophy of the pancreas, kyphoscoliosis, and pes cavus (review in ref. ). Ataxia, dysarthria, dysmetria, dysphagia, weakness, flaccidity or spasticity, areflexia or hyperreflexia, peripheral neuropathy, hearing loss, visual impairment, and cognitive disability combine to form a severe neurological phenotype. The main cause of death, however, is cardiomyopathy. The reason for such diversity of lesions remains unknown, but lack of iron sulfur cluster (ISC) biosynthesis, incomplete ISC transfer to ISC-dependent proteins, deficient mitochondrial ATP production, and insufficient antioxidant defenses are under consideration for all affected tissues.
FA is often called a degenerative disease of neurons, including those of DRG, though frataxin deficiency also affects supporting cells. This report presents evidence for a primary disturbance of satellite cells and a role of inflammation in the destruction of DRG neurons in FA.
The principal methods in this work were immunohistochemistry and immunofluorescence with validated antibodies against structural and channel proteins of satellite cells and against inflammatory proteins. The current study benefitted from published data in experimental animals [4, 6, 7, 9, 17, 19, 22] and sought to establish relevant information in normal human DRG and the DRG in FA.
Materials and methods
Clinical data and autopsy specimens
The Institutional Review Board of the Veterans Affairs Medical Center in Albany, NY, USA, has approved the research described in this paper. DRG of 15 FA patients and 12 normal controls were available for routine staining of paraffin-embedded tissue sections, immunohistochemistry, and immunofluorescence. Tissues of FA patients were collected under a formal donation program supported by Friedreich’s Ataxia Research Alliance. Control DRG were obtained during autopsies conducted at Veterans Affairs Medical Center and Albany Medical College; and from National Disease Research Interchange, Philadelphia, PA, USA. Detailed clinical and genetic information was available for all patients (8 male, 7 female). Age of onset ranged from 2 to 18 years (mean ± standard deviation [S.D.]: 10 ± 5), and age of death from 10 to 69 years (mean ± S.D.: 36 ± 18). All patients had homozygous GAA repeat expansions, ranging from 249 to 1200 for GAA1 and 566–1200 for GAA2 (means ± S.D.: GAA1, 734 ± 251; GAA2, 955 ± 204). Autopsy delays were 2–96 h. The age range of the controls (9 male, 3 female) was 48–68 years (mean ± S.D.: 60 ± 6). Autopsy delays in the control cases ranged from 1 to 48 h.
Immunohistochemistry and immunofluorescence
Antigenic proteins, antibodies, and antigen retrieval methods
Protein concentration (μg/ml) or dilution for IHC or IF
Antigen retrieval method
0.8 (IHC and IF)
tris (IHC), DIVA (IF)
0.75 (IHC and IF)
chelation and DIVA
citrate (IHC), DIVA (IF)
0.4 (IHC), 0.8 (IF)
We used several methods to validate the identity of proteins in tissue sections. Four proteins listed in Table 1 (ATP5B, connexin 43, EAAT1, and IBA1) were validated by tandem mass spectrometry including multiple reaction monitoring-initiated detection and sequencing (MIDAS), as described by Unwin et al. . For this purpose, proteins in samples of frozen DRG (35–65 mg) were extracted by ultrasonication in a buffer found suitable for the assay of tissue frataxin [5, 16]. After centrifugation at 14,000 × g for 45 min, the supernatant was collected, and an aliquot was used for sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting by established procedures. The apparent molecular weights of the detected protein bands were calculated by reference to a series of prestained standard proteins and compared with information on mass in UniProt . The protein bands on matching Coomassie Blue-stained gels were excised for MIDAS. One or more signature peptides were identified for each of the four aforementioned proteins by in-gel trypsin digestion, alkylation, and MIDAS at the Center for Functional Genomics at State University of New York at Albany, Rensselaer, NY, USA. The authenticity of staining with antibodies to frataxin, laminin, glutamine synthetase, mGluR2/3, Kir4.1, CD68, and cytosolic ferritin was supported by absorption of the antibodies with the corresponding purified antigenic protein, recombinant protein, or antigenic peptide (frataxin: recombinant human protein, courtesy of Dr. Grazia Isaya, Mayo Clinic, Rochester, MN, USA; laminin: purified laminin, AbD Serotec, Raleigh, NC, USA, Cat. No. 5620–0604; glutamine synthetase: full length recombinant protein, Abcam, Cambridge, MA, USA, Cat. No. ab98145; mGluR2/3: peptide GREVVDSTTSSL, US Biological, Salem, MA, USA, Cat. No. M3884-76C; Kir4.1: antigenic peptide CKLEESLREQAEKEGSALSVR, Alomone Labs, Jerusalem, Israel, Cat. No. APC-035; CD68: recombinant protein, Abcam, Cat. No. ab38260; cytosolic ferritin: purified human liver ferritin, EMD-Millipore, Billerica, MA, USA, Cat. No. 341482). To further characterize S100 reaction products, we used a monovalent polyclonal anti-S100α antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA, Cat. No. SC-7849-R) and absorption of the monoclonal anti-S100 antibody by an excess of recombinant S100α (Abnova, Taipei, Taiwan, Cat. No. H00006271-P01) or full-length S100β-peptide (Abcam, Cat. No. ab30380).
Structural proteins of satellite cells and gap junction protein connexin 43
Channel proteins of satellite cells
Inflammatory cells and ferritin expression
Figure 5b and d confirm the localization of cytosolic ferritin in the cytoplasm of neurons and satellite cells in DRG. Though the most intense red ferritin fluorescence arises from satellite cells, the yellow color on merged images in Fig. 5b and d suggests that IBA1-positive monocytes also contain this protein. Figure 5d of a DRG from an FA patient with a rapidly progressive course shows the great abundance of ferritin-positive satellite cells. The insets confirm the colocalization of IBA1 and cytosolic ferritin.
Optimal preservation of tissue antigens depends on prompt fixation after death, which was difficult to achieve with autopsy delays beyond a few hours. It was evident that in some DRG samples, autolysis introduced a bias in the interpretation of immunohistochemistry of the more perishable antigenic determinants, and antigen retrieval methods (Table 1) did not always assure detection. Also, autolysis precluded ultrastructural study of satellite cells in FA.
The presence of reaction product does not necessarily provide information on satellite cell function or dysfunction. The emergence of tissue-based proteomics with multiple validated antibodies [7, 25], however, supports the conclusion that abnormal satellite cell structure correlates with expression changes in FA-related proteins.
Satellite cell proliferation in FA
Invariably, FA causes hypercellularity of DRG, and immunohistochemical stains illustrated in Figs. 1, 2 and 3 confirm hypertrophy and hyperplasia of satellite cells. Residual nodules, also called nodules of Nageotte, retain immunoreactivity with antibodies to glutamine synthetase (Fig. 1d) and S100 (Fig. 1e) though their constituent cells have lost contact with DRG neurons. The differential expression of S100 in satellite cells (S100α and S100β) and nearby Schwann cells (S100β) is unexplained, but derivation of satellite cells from boundary cap cells may account for the dissimilar distribution of the S100 isoproteins . Laminin reaction product displayed in Fig. 1f indicates that frataxin deficiency in FA causes the formation of redundant satellite cell processes in addition to an obvious numerical increase of these cells. Stains of glial fibrillary acidic protein and vimentin (not illustrated) resemble those of glutamine synthetase and S100. In none of the available DRG of 15 patients with FA did hematoxylin and eosin stains reveal mitoses, and immunohistochemistry with a monoclonal antibody to the KI-67 antigen was negative. Mitoses are present in developing DRG , but authors of systematic immunohistochemical studies of DRG in animals that underwent experimental manipulations did not comment on mitotic figures in proliferating satellite cells [4, 6]. Neuronophagic invasion (Fig. 4c and d) suggests an active process of nerve cell destruction that may be independent of slower satellite cell proliferation.
The greater abundance of connexin 43-reactive gap junctions in FA (Fig. 2e and g) correlates well with the increased number of satellite cell processes (Fig. 1f). Normal DRG neurons and their satellite cell layers may be viewed as units. When satellite cells proliferate, their processes contact cells surrounding neighboring neurons and form new gap junctions . This sequence of events is apparent in FA (Fig. 2e). The biochemical stimulus to generate more gap junctions is not specific for FA, as a numerical increase of gap junctions occurs adult mice undergoing sciatic nerve transection .
Glutamate and potassium flux in satellite cells of FA
Glutamate in DRG, glutamate recycling, and glutamate transport have received attention in pain processing by DRG in experimental animals [3, 17, 19] and in mixed neuronal/glial cell cultures . The histochemical visualization of vesicular glutamate transporters in the neurons of DRG  implies biosynthesis and vesicular packaging of the neurohumoral transmitter in the perikarya of DRG neurons, though glutamatergic synaptic contacts occur at a distance in the spinal cord gray matter. Visualization of glutamine synthetase and EAAT1 in normal DRG (Figs. 1a and 3b, respectively) supports the hypothesis that neuronal glutamate recycling in DRG takes place in a transmembrane exchange between nerve and satellite cells . A similar claim may be made for potassium flux control by Kir4.1 (Fig. 3c). The expression of mGluR2/3 in satellite cells (Fig. 3a), however, is more difficult to reconcile with neurohumoral transmission. Carlton and Hargett  suggested “modulation” of glutamatergic activity in DRG by mGluR. The persistence of glutamine synthetase, EAAT1, Kir4.1, and mGluR2/3 immunoreactivity in proliferating satellite cells provides only limited insight beyond a general disturbance of glutamate and potassium homeostasis in FA. In theory, glutamate dysmetabolism in DRG should cause more pain, which is not consistent with the moderate neuropathic symptoms in FA.
Inflammatory infiltration of DRG in FA and ferritin expression in satellite cells
In his comprehensive review of sensory ganglia in animals, Pannese (; page 61) commented only briefly on the presence of macrophages in normal DRG. Later studies utilizing immunohistochemistry of major histocompatibility complex II (MHCII) and ED-1 (a monoclonal antibody to CD68) confirmed the invariable presence of resident monocytes in DRG of normal animals [6, 9]. In the current study, CD68 (Fig. 4a) and IBA1 (Fig. 4b) reaction products indicate that resident monocytes are present and quite numerous in normal human DRG. The dissimilar expression of CD68 and IBA1 is unexplained. The discrepancy may be due to technical factors or the result of differential clonal expression. Dubový et al.  reported that in normal rat DRG, macrophages did not establish intimate contact with neurons. Sciatic nerve transection changed this relationship, and macrophages became neuronal “satellite cells”. It is unlikely, however, that cells expressing CD11, CD14, CD45, CD68, and MHCII in human trigeminal ganglia are actually neural crest-derived satellite cells . The current study does not support expression of monocyte markers by satellite cells. While monocytes gain access to the satellite cell layer surrounding normal human DRG and mingle with satellite cells, S100 and IBA1 reaction products do not co-localize (Fig. 5a). Monocytes may lie very close to the neuronal plasma membrane in normal DRG, but high-resolution confocal immunofluorescence shows that they remain separated from the nerve cell (Fig. 5a). In FA, mononuclear cells appear to penetrate the neuronal cytoplasm in FA (Figs. 4d and 5c) though electron microscopy would be required for definitive proof of transmembrane migration. Monocytes also participate in the formation of residual nodules (Fig. 5c, inset). Cells with either CD68- or IBA1-expression participate in the destruction of DRG neurons (Fig. 4c and d, respectively). We did not observe polymorphonuclear leukocytes, CD3-, or CD20-positive lymphocytes in the inflammatory infiltrate of DRG in FA.
In FA, upregulation of ferritin biosynthesis in proliferating satellite cells is the likely response to Fe leakage from DRG neurons (Fig. 5d and ref ). It is remarkable that the main mechanism of ferritin biosynthesis is located in satellite cells rather than monocytes. These leukocytes and their derivative macrophages are fully capable of translating ferritin messenger ribonucleic acids in response to local Fe excess, and IBA1-reactive monocytes among proliferating satellite cells in FA are also ferritin-reactive (Fig. 5d, insets). Based on quantitative in-situ X-ray fluorescence, total DRG Fe in FA does not change, and retention of the metal is due to Fe uptake by satellite cells . A similar process was also proposed for the transfer of Zn from DRG neurons to satellite cells, triggering the biosynthesis of metallothioneins 1 and 2 . The evidence for a primary role of the two transition metals, Fe and Zn, in oxidative damage to DRG in FA remains inconclusive.
Mitochondria in the satellite cells of DRG in FA
FA is a recognized mitochondrial disease, and the greater number of mitochondria in satellite cells requires an explanation beyond metabolic activation. It is plausible that the mitochondrial abundance is a compensatory, and likely maladaptive, response to oxidative stress, energy deficiency, or both (review in ref. ). Mitochondria are a source of reactive oxygen species, and an inappropriate number of these organelles in satellite cells may actually cause further damage to the cell. One conclusion of the work presented here is that frataxin deficiency does not impair mitochondrial biogenesis, but electron microscopy will be necessary to determine whether the ultrastructure of the “new” mitochondria in DRG of FA is entirely normal.
Pathogenesis of the DRG lesion in FA
The literature contains few reports of normal or diseased human DRG that include immunohistochemistry and immunofluorescence. The rationale for our continuing work is to identify satellite cell proteins that undergo distinct changes in expression due to FA. The ultimate goal is to develop a more comprehensive tissue-based proteomic analysis  to measure the downstream effects of frataxin deficiency. Animal models of FA show vacuolation of DRG neurons [2, 23] but lack proliferation of satellite cells and residual nodules. From the published light-microscopic images of DRG in mouse models [2, 23], it cannot be inferred whether some of the cells surrounding vacuolated neurons are inflammatory. Pannese et al.  established that in DRG of cats and rabbits the volume of satellite cells is linearly related to volume and surface area of neurons. Rats and guinea pigs were not suitable for a comparable analysis because they did not meet the requirement of an approximately spherical shape of DRG neurons. For this reason, murine models of FA may not be suitable for a comparable study.
Visualization of satellite cell cytoplasm by multiple stains (Figs. 1, 2 and 3) leaves little doubt that the ratio of satellite cell volume to neuronal surface area in DRG of human FA is greatly increased. Hypertrophy of satellite cells (Fig. 1e), abundance of laminin (Fig. 1f), and upregulated mitochondriogenesis (Fig. 6c-d) militate against the interpretation that DRG hypercellularity is a passive phenomenon and simply due to collapse of ganglion cells. The functional consequences of excess satellite cell cytoplasm are unknown. A long-standing hypothesis states that satellite cells provide trophic support to DRG neurons [20, 21]. Nerve cells may also signal satellite cells, making trophic support bidirectional. It cannot be assumed that frataxin deficiency primarily impairs neurons. It is equally possible that satellite cell function fails and that atrophy and inflammatory invasion of neurons are secondary events.
A critical question in the pathogenesis of the DRG lesion in FA is: When does the disease process in DRG begin? The answer may arise from the following observations: The youngest patient in our group of 15, a boy, began to show symptoms at the age of 2 years and survived for only 8 more years because of aggressive FA cardiomyopathy. His DRG revealed neuronal atrophy, satellite cell proliferation, and inflammatory infiltration that were comparable to cases of much later onset. A systematic comparison of neuronal sizes in DRG of FA patients with juvenile-onset (< 20 years) and long survival (> 20 years), and late-onset (> 20 years) and long survival (> 20 years), did not reveal significant differences . It may be concluded that quantifiable atrophy of DRG neurons does not correlate significantly with disease onset. In-situ hybridization of mouse embryos to detect frataxin messenger ribonucleic acid shows strong early expression in DRG , suggesting high metabolic demand for this protein and early, perhaps prenatal, vulnerability to FA.
Frataxin deficiency causes severe changes in sensory peripheral nerves , and it is uncertain that FA neuropathy is solely the result of the lesion in DRG. In theory, sensory neuropathy in FA may be a primary effect of the disease, contributing to the damage in DRG.
Systemic or targeted replacement of frataxin is an obvious goal of therapy in FA. Koeppen et al.  expressed the opinion that FA causes hypoplasia and superimposed atrophy of DRG. Since onset of ataxia in FA does not correlate with the degree of neuronal atrophy in DRG, restoration of frataxin can only be expected to preserve residual DRG function. In contrast to DRG, neuronal loss in the dentate nucleus is progressive and more closely related to disease onset [14, 16]. The blood–brain barrier may block the delivery of frataxin protein or viral carriers of the FXN gene to the DN, but cells of DRG and sensory nerves should be more accessible. It is unknown whether anti-inflammatory drugs would improve survival of DRG neurons and benefit patients with FA neuropathy and sensory ataxia.
Systematic immunohistochemistry and immunofluorescence of structural and channel proteins, and of proteins related to inflammation, permits a new interpretation of FA-related changes in DRG. The disease process in FA affects satellite cell independently and is not solely due to atrophy of DRG neurons. Satellite cells undergo hypertrophy and hyperplasia, maladaptive mitochondriogenesis, and enhanced ferritin expression. DRG are infiltrated by inflammatory cells that participate in the destruction of neurons similar to neuronophagia in the central nervous system. It is likely that frataxin deficiency causes loss of bidirectional trophic support of satellite cells and neurons.
We are grateful to the families of patients with Friedreich ataxia who graciously allowed autopsies and the collection of tissues for research. The described work was supported by grants to AHK by National Institutes of Health (NIH) (R01 NS069454) and Friedreich’s Ataxia Research Alliance. We also acknowledge the use of control tissues procured by National Disease Research Interchange (NDRI). NDRI is supported by NIH grant U42 OD0111158. The described research has been performed in the laboratories of Veterans Affairs Medical Center, Albany, NY, USA. Dr. Qishan Lin of Center for Functional Genomics at State University of New York at Albany performed the MIDAS experiments and advised the authors on the interpretation of the results.
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- Albuerne M, Mammola CL, Naves FJ, Levanti B, Germanà G, Vega JA. Immunohistochemical localization of S100 proteins in dorsal root, sympathetic and enteric ganglia of several mammalian species, including man. J Peripher Nerv Syst. 1998;3:243–53.PubMedGoogle Scholar
- Al-Mahdawi S, Pinto RM, Varshney D, Lawrence L, Lowrie MB, et al. GAA repeat expansion mutation mouse models of Friedreich ataxia exhibit oxidative stress leading to progressive neuronal and cardiac pathology. Genomics. 2006;88:580–90.View ArticlePubMedPubMed CentralGoogle Scholar
- Brumovsky P. VGLUTs in peripheral neurons and the spinal cord: time for a review. ISRN Neurol. 2013. http://dx.doi.org/10.1155/2013/829753.
- Carlton SM, Hargett GL. Colocalization of metabotropic glutamate receptor in rat dorsal root ganglion cells. J Comp Neurol. 2007;501:780–9.View ArticlePubMedGoogle Scholar
- Condò I, Ventura N, Malisan F, Tomassini B, Testi R. A pool of extramitochondrial frataxin that promotes cell survival. J Biol Chem. 2006;281:1750–6.View ArticleGoogle Scholar
- Dubový P, Tučková L, Jančálek R, Svίženská I, Klusáková I. Increased invasion of ED-1 positive macrophages in both ipsi- and contralateral dorsal root ganglia following unilateral nerve injuries. Neurosci Lett. 2007;427:88–93.View ArticlePubMedGoogle Scholar
- Hanani M. Satellite glial cells in sensory ganglia: from form to function. Brain Res Rev. 2005;48:457–76.View ArticlePubMedGoogle Scholar
- Hanani M, Huang TY, Cherkas PS, Ledda M, Pannese E. Glial cell plasticity in sensory ganglia induced by nerve damage. Neuroscience. 2002;114:279–83.View ArticlePubMedGoogle Scholar
- Hu P, McLachlan EM. Macrophage and lymphocyte invasion of dorsal root ganglia after peripheral nerve lesions in the rat. Neuroscience. 2002;112:23–38.View ArticlePubMedGoogle Scholar
- Jiralerspong S, Liu Y, Montermini L, Stifani S, Pandolfo M. Frataxin shows developmentally regulated tissue-specific expression in the mouse embryo. Neurobiol Dis. 1997;4:103–13.View ArticlePubMedGoogle Scholar
- Koeppen AH. Friedreich’s ataxia: pathology, pathogenesis, and molecular genetics. J Neurol Sci. 2011;303:1–12.View ArticlePubMedPubMed CentralGoogle Scholar
- Koeppen AH, Mazurkiewicz JE. Friedreich ataxia: neuropathology revised. J Neuropathol Exp Neurol. 2013;72:78–90.View ArticlePubMedGoogle Scholar
- Koeppen AH, Morral JA, Davis AN, Qian J, Petrocine SV, Knutson MD, et al. The dorsal root ganglion in Friedreich’s ataxia. Acta Neuropathol. 2009;118:763–76.View ArticlePubMedGoogle Scholar
- Koeppen AH, Morral JA, McComb RD, Feustel PJ. The neuropathology of late-onset Friedreich’s ataxia. Cerebellum. 2011;10:96–103.View ArticlePubMedGoogle Scholar
- Koeppen AH, Kuntzsch EC, Bjork ST, Ramirez RL, Mazurkiewicz JE, Feustel PJ. Friedreich ataxia: metal dysmetabolism in dorsal root ganglia. Acta Neuropathol Commun. 2013;1:26.View ArticlePubMedPubMed CentralGoogle Scholar
- Koeppen AH, Ramirez RL, Becker AB, Feustel PJ, Mazurkiewicz JE. Friedreich ataxia: failure of GABA-ergic and glycinergic synaptic transmission in the dentate nucleus. J Neuropathol Exp Neurol. 2015;74:166–76.View ArticlePubMedPubMed CentralGoogle Scholar
- Kung L-H, Gong K, Adedoyin M, Ng J, Bhargava A, Ohara PT, et al. Evidence for glutamate as a neuroglial transmitter within sensory ganglia. PLoS ONE. 2013;8, e68312.View ArticlePubMedPubMed CentralGoogle Scholar
- Morral JA, Davis AN, Qian J, Gelman BB, Koeppen AH. Pathology and pathogenesis of sensory neuropathy in Friedreich’s ataxia. Acta Neuropathol. 2010;120:97–108.View ArticlePubMedGoogle Scholar
- Ohara PT, Vit J-P, Bhargava A, Romero M, Sundberg C, Charles AC, et al. Gliopathic pain: when satellite glia cells go bad. Neuroscientist. 2009;15:450–63.View ArticlePubMedPubMed CentralGoogle Scholar
- Pannese E. The satellite cells of the sensory ganglia. Berlin: Springer; 1981.View ArticleGoogle Scholar
- Pannese E, Bianchi R, Calligaris B, Ventura R, Weibel ER. Quantitative relationships between nerve and satellite cells in spinal ganglia. An electron microscopical study. I. Mammals. Brain Res. 1972;46:215–34.View ArticlePubMedGoogle Scholar
- Procacci P, Magnaghi V, Pannese E. Perineuronal satellite cells in mouse spinal ganglia express the gap junction protein connexin43 throughout life with decline in old age. Brain Res Bull. 2008;75:562–9.View ArticlePubMedGoogle Scholar
- Simon D, Seznec H, Gansmuller A, Carelle N, Weber P, et al. Friedreich ataxia mouse models with progressive cerebellar and sensory ataxia reveal autophagic neurodegeneration in dorsal root ganglia. J Neurosci. 2004;24:1987–95.View ArticlePubMedGoogle Scholar
- Topilko P. Les cellules des capsules frontières-une niche de cellules souches neurales dans le système nerveux périphérique. Bull Acad Natl Méd. 2007;191:1383–94.PubMedGoogle Scholar
- Uhlén M, Fagerberg L, Hallström BM, Lindskog C, Oksvold P, Mardinoglu A, et al. Tissue-based map of the human proteome. Science. 2015;347(6220):1260419.View ArticlePubMedGoogle Scholar
- Uittenbogaard M, Chiaramello A. Mitochondrial biogenesis: a therapeutic target for neurodevelopmental disorders and neurodegenerative diseases. Curr Pharm Des. 2014;20:5574–93.View ArticlePubMedPubMed CentralGoogle Scholar
- The UniProt Consortium. UniProt: a hub for protein information. Nucleic Acids Res. 2015;43(Database issue):D204–12.View ArticlePubMed CentralGoogle Scholar
- Unwin RD, Griffiths JR, Whetton AD. A sensitive mass spectrometric method for hypothesis-driven detection of peptide post-translational modifications: multiple reaction monitoring-initiated detection and sequencing (MIDAS). Nat Protoc. 2009;4:870–7.View ArticlePubMedGoogle Scholar
- Van Velzen M, Laman JD, Klein-Jan A, Poot A, Osterhaus ADME, Verjans GMGM. Neuron-interacting satellite glial cells in human trigeminal ganglia have an APC phenotype. J Immunol. 2009;183:2456–61.View ArticlePubMedGoogle Scholar
- Vankan P. Prevalence gradients of Friedreich’s ataxia and R1b haplotype in Europe co-localize, suggesting a common Paleolithic origin in the Franco-Cantabrian ice age refuge. J Neurochem. 2013;126 suppl 1:11–20.View ArticlePubMedGoogle Scholar