Dorsal root ganglia in Friedreich ataxia: satellite cell proliferation and inflammation

Table 1 Antigenic proteins, antibodies, and antigen retrieval methods

Antigenic protein	Antibody supplier	Catalogue number	Host	Clonality (isotype)	Protein concentration (μg/ml) or dilution for IHC or IF	Antigen retrieval method
ATP5B	Santa Cruz	sc-33618	mouse	monoclonal (IgG)	0.8 (IHC and IF)	tris (IHC), DIVA (IF)
CD68	Santa Cruz	sc-20060	mouse	monoclonal (IgG)	4 (IHC)	citrate
Connexin 43	Abcam	ab113470	rabbit	polyclonal (IgG)	0.75 (IHC and IF)	citrate
Cytosolic ferritin	GenWay	GWB-B74CB5	goat	polyclonal (IgG)	4 (IF)	chelation and DIVA
EAAT1/GLAST1	Santa Cruz	sc-77557	goat	polyclonal (IgG)	0.5 (IHC)	citrate
Frataxin	Abcam	ab110328	mouse	monoclonal (IgG)	10 (IHC)	tris
Glutamine synthetase	Santa Cruz	sc-74430	mouse	monoclonal (IgG)	1 (IHC)	citrate
IBA1/AIF1	Sigma	HPA049234	rabbit	polyclonal (IgG)	1 (IHC)	citrate (IHC), DIVA (IF)
Kir4.1	Alomone	APC-035	rabbit	polyclonal (IgG)	4 (IHC)	DIVA
Laminin	Sigma	L8271	mouse	monoclonal (IgG)	1:1000 (IHC)	proteinase K
mGluR2/3	Novus	NBPI-00924	rabbit	polyclonal ((IgG)	1 (IHC)	DIVA
S100	Santa Cruz	sc-53438	mouse	monoclonal (IgG)	0.4 (IHC), 0.8 (IF)	citrate
S100α	Santa Cruz	Sc-7849-R	rabbit	polyclonal (IgG)	0.4 (IHC)	citrate

Abbreviations and notes: Abcam Cambridge, MA, USA, AIF1 allograft inflammatory factor 1 (synonym for IBA1), Alomone Alomone Labs, Jerusalem, Israel, ATP5B ATP synthase subunit beta, mitochondrial, Chelation preincubation of slides in 30 mM each of 2,2′-dipyridyl and sodium hydrosulfite in 0.1 M acetic acid-sodium acetate buffer, pH 4.8, Citrate 0.01 M citric acid/sodium citrate, pH 6, 10 min at 95 °C, 10 min cool-down period to room temperature, DIVA a proprietary antigen retrieval solution (Biocare Medical, Concord, CA, USA), diluted 1:10, 30 min at 95 °C, EAAT1 excitatory amino acid transporter 1; synonym: GLAST1, glutamate aspartate transporter 1, GenWay GenWay Biotech, San Diego, CA, USA, IF immunofluorescence, IHC immunohistochemistry, Kir4.1 ATP-sensitive inward rectifier potassium channel 10; gene product of KCNJ10, Laminin ascites fluid, mGluR2/3 metabotropic glutamate receptors 2 and 3. The supplier reports that the polyclonal antibody raised against an mGluR2-specific peptide also reacts with mGluR3. mGluR2 and mGluR3 show 68 % sequence homology, Novus Novus Biologicals, Littleton, CO, Proteinase K 0.1 mg/ml, 0.1 M NaCl, 20 mM tris, pH 8, 30 min at 37 °C, Santa Cruz Santa Cruz Biotechnology, Santa Cruz, CA, USA, Sigma Sigma-Aldrich, St. Louis, MO, USA, Tris 0.1 M tris buffer, pH 9.6, 5 % urea (w/vol), 20 min at 95 °C

ISSN: 2051-5960