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Table 1 Antigenic proteins, antibodies, and antigen retrieval methods

From: Dorsal root ganglia in Friedreich ataxia: satellite cell proliferation and inflammation

Antigenic protein Antibody supplier Catalogue number Host Clonality (isotype) Protein concentration (μg/ml) or dilution for IHC or IF Antigen retrieval method
ATP5B Santa Cruz sc-33618 mouse monoclonal (IgG) 0.8 (IHC and IF) tris (IHC), DIVA (IF)
CD68 Santa Cruz sc-20060 mouse monoclonal (IgG) 4 (IHC) citrate
Connexin 43 Abcam ab113470 rabbit polyclonal (IgG) 0.75 (IHC and IF) citrate
Cytosolic ferritin GenWay GWB-B74CB5 goat polyclonal (IgG) 4 (IF) chelation and DIVA
EAAT1/GLAST1 Santa Cruz sc-77557 goat polyclonal (IgG) 0.5 (IHC) citrate
Frataxin Abcam ab110328 mouse monoclonal (IgG) 10 (IHC) tris
Glutamine synthetase Santa Cruz sc-74430 mouse monoclonal (IgG) 1 (IHC) citrate
IBA1/AIF1 Sigma HPA049234 rabbit polyclonal (IgG) 1 (IHC) citrate (IHC), DIVA (IF)
Kir4.1 Alomone APC-035 rabbit polyclonal (IgG) 4 (IHC) DIVA
Laminin Sigma L8271 mouse monoclonal (IgG) 1:1000 (IHC) proteinase K
mGluR2/3 Novus NBPI-00924 rabbit polyclonal ((IgG) 1 (IHC) DIVA
S100 Santa Cruz sc-53438 mouse monoclonal (IgG) 0.4 (IHC), 0.8 (IF) citrate
S100α Santa Cruz Sc-7849-R rabbit polyclonal (IgG) 0.4 (IHC) citrate
  1. Abbreviations and notes: Abcam Cambridge, MA, USA, AIF1 allograft inflammatory factor 1 (synonym for IBA1), Alomone Alomone Labs, Jerusalem, Israel, ATP5B ATP synthase subunit beta, mitochondrial, Chelation preincubation of slides in 30 mM each of 2,2′-dipyridyl and sodium hydrosulfite in 0.1 M acetic acid-sodium acetate buffer, pH 4.8, Citrate 0.01 M citric acid/sodium citrate, pH 6, 10 min at 95 °C, 10 min cool-down period to room temperature, DIVA a proprietary antigen retrieval solution (Biocare Medical, Concord, CA, USA), diluted 1:10, 30 min at 95 °C, EAAT1 excitatory amino acid transporter 1; synonym: GLAST1, glutamate aspartate transporter 1, GenWay GenWay Biotech, San Diego, CA, USA, IF immunofluorescence, IHC immunohistochemistry, Kir4.1 ATP-sensitive inward rectifier potassium channel 10; gene product of KCNJ10, Laminin ascites fluid, mGluR2/3 metabotropic glutamate receptors 2 and 3. The supplier reports that the polyclonal antibody raised against an mGluR2-specific peptide also reacts with mGluR3. mGluR2 and mGluR3 show 68 % sequence homology, Novus Novus Biologicals, Littleton, CO, Proteinase K 0.1 mg/ml, 0.1 M NaCl, 20 mM tris, pH 8, 30 min at 37 °C, Santa Cruz Santa Cruz Biotechnology, Santa Cruz, CA, USA, Sigma Sigma-Aldrich, St. Louis, MO, USA, Tris 0.1 M tris buffer, pH 9.6, 5 % urea (w/vol), 20 min at 95 °C