The catalytic activity of human Telomerase Reverse Transcriptase (TERT) compensates for the loss of telomere length, eroded during each cell cycle, to ensure a correct division of stem and germinal cells. In human tumors, ectopic TERT reactivation, most frequently due to hotspot mutations in the promoter region (TERTp), i.e. c.1-124 C > T, c.1-146 C > T, confers a proliferative advantage to neoplastic cells. In gliomas, TERTp mutations (TERTpmut) mainly occur in oligodendroglioma and glioblastoma. We screened, for TERTp hotspot mutations, 301 adult patients with gliomas and identified heterozygous mutations in 239 cases: 94% of oligodendroglioma, 85% of glioblastoma, and 37.5% of diffuse/anaplastic astrocytoma. Besides the recurrent c.1-124 C > T and c.1-146 C > T, two cases of glioblastoma harbored novel somatic TERTp variants, which consisted of a tandem duplications of 22 nucleotides, i.e. a TERTp c.1-100_1-79dup and TERTp c.1-110_1-89, both located downstream c.1-124 C > T and c.1-146 C > T. In silico analysis predicted the formation of 119 and 108 new transcription factor’s recognition sites for TERTp c.1-100_1-79dup and TERTp c.1-110_1-89, respectively. TERTp duplications (TERTpdup) mainly affected the binding capacity of two transcription factors’ families, i.e. the members of the E-twenty-six and the Specificity Protein/Krüppel-Like Factor groups. In fact, these new TERTpdup significantly enhanced the E-twenty-six transcription factors’ binding capacity, which is also typically increased by the two c.1-124 C > T/c.1-146 C > T hotspot TERTpmut. On the other hand, they were distinguished by enhanced affinity for the Krüppel proteins. The luciferase assay confirmed that TERTpdup behaved as gain-of-function mutations causing a 2,3-2,5 fold increase of TERT transcription. The present study provides new insights into TERTp mutational spectrum occurring in central nervous system tumors, with the identification of new recurrent somatic gain-of-function mutations, occurring in 0.8% of glioblastoma IDH-wildtype.
The abnormal reactivation of human Telomerase Reverse Transcriptase (TERT) is a common hallmark of human solid tumors. Although it may be caused by several mechanisms, i.e. methylation, mutations, rearrangements/fusions, and DNA copy number amplifications, TERT promoter (TERTp) methylation, and gain-of-function mutations are the most frequent [2, 28]. In particular, two recurrent hotspot mutations are respectively located at -124 (TERTp-124) and -146 (TERTp-146) base pairs (bp), from the TERT ATG start site [2, 10,11,12, 28]. Both mutations, generated from a cytidine to thymidine dipyrimide transition (C > T), are usually heterozygous, mutually exclusive, and produce an identical 11 bp ‘CCCCTTCCGGG’ sequence, resulting in the creation of de novo consensus binding motifs for E-twenty-six (ETS) transcription family members. These new binding sites recruit a larger number of ETS factors, enhancing the transcription of TERT .
TERT promoter mutations (TERTpmut) typically occur in tumors that arise from low self-renewal tissue, such as melanomas, thyroid, hepatobiliary carcinoma, and central nervous system (CNS) tumors, with a variable frequency, that range from 15 to 90% of cases, in diverse histological subtypes [10, 14, 28]. In CNS tumors, TERTpmut are typically associated with glioblastoma (GBM) (70–80%) and oligodendroglioma (ODG) (60–70%), whereas their frequency decreases in other glioma subtypes, such as diffuse/anaplastic astrocytoma (DA/AA) (30–40%), medulloblastoma (20-30%), and meningioma (about 7%) [10, 25, 27]. Although the clinical value of TERTpmut, in refining the diagnostic classification of gliomas, is widely accepted , its role as prognostic/predictive biomarker is still largely debated. TERTpmut have been associated with a poor disease outcome in GBM IDH-wildtype (GBM IDHwt), but there is no full agreement on its impact on DA/AA [6, 15, 16, 22, 24, 29]. It is worth noting, however, that DA/AA IDH-wildtype (DA/AA IDHwt) harboring genomic abnormalities typically associated with GBM, i.e. TERTp mutations, or EGFR amplification, or gain of whole chromosome 7 in combination with monosomy of chromosome 10, have a clinical outcome similar to, or only slightly longer, than GBM . Thus, the cIMPACT NOW (Update 3) recommended to use one of these molecular criteria to classify this subgroup of astrocytomas as “diffuse astrocytic glioma, IDH-wildtype, with molecular features of glioblastoma, WHO grade IV” and to revise the classification of DA/AA IDHwt, accordingly .
Herein, we report two new TERTp mutations that were identified in two patients with GBM IDHwt. Both these new variants originated from the duplication of a stretch of 22 nucleotides at TERTp (TERTpdup) and, although slightly different, shared an overlapping sequence of 12 nucleotides. We demonstrated the somatic nature of one of these TERTpdup and that, enhancing the binding affinity for ETS transcription factors (TFs), they both elicit the TERT transcription, thus widening the spectrum of recurrent gain-of-function mutations of TERTp in GBM.
The study was carried out on a cohort of 301 patients, affected by primary CNS tumours, and referred to our laboratory during the last 10 years (Table 1). There were 175 males and 126 females (ratio 1.4:1) with a median age of 64 (range age: 20-86). According to the WHO 2016, the diagnosis was: grade II DA IDHwt (6 cases) and DA IDH-mutant (DA IDHmut) (10 cases); grade III AA IDHwt (6 cases) and AA IDHmut (= 10); grade IV GBM IDHwt (= 241) and GBM IDHmut (= 10); grade II/III ODG (= 15). Three patients had a diagnosis of uncommon glioma (Table 1). The study was approved by Institutional Bioethics Committee (University of Perugia and Santa Maria della Misericordia Hospital of Perugia-Italy, Protocol no.2843/16); all patients gave informed consent for sample collection and molecular analyses, in agreement with the Declaration of Helsinki.
A 71-year-old male (UPN#131) had a left frontal lesion of 24 mm diameter, partially infiltrating the corpus callosum; the second case (UPN#171), a male of 78 years, presented with a right frontal lesion. Histopathology and immunohistochemistry were consistent with a diagnosis of GBM IDHwt, in both patients. In case UPN#131, neoplastic cells showed marked cytoplasmic and nuclear pleomorphism; there was a discrete number of atypical mitotic figures, widespread necrosis, a diffuse GFAP positivity (100%), and few neoplastic elements (20%) with strong nuclear TP53 stain. Case UPN#171, was characterized by striking atypia of neoplastic cells, diffuse necrosis, vascular proliferation, strong and diffuse positivity for GFAP and nuclear TP53 (> 70%) (Fig. 1). No IDH1/IDH2 hotspot mutations were detected, while both cases showed MGMT promoter methylation. Monosomy of chromosome 10 co-occurred with EGFR amplification (UPN#131) or with gain of the whole chromosome 7 (UPN#171).
Materials and methods
TERT promoter mutational analysis
Genomic DNA was extracted from Formalin-Fixed Paraffin-Embedded (FFPE) tumor tissue and from peripheral blood (PB) by QIAamp DNA FFPE and AllPrep DNA/RNA kits, respectively, following the manufacturer’s instructions (QIAGEN, Milan, Italy). Hotspot TERTpmut were investigated by Sanger sequencing using ABI 3500 Genetic analyzer instrument (Applied Biosystems, Monza, Italy). Primers were reported in Table S1 (Additional file 1: Table S1) and referred to GRCh37 genomic coordinate system (NM_000005.9, for regulatory core promoter 274 bp) (www.ncbi.nlm.nih.gov/gene , www.ensembl.org/Homo_sapiens ). Sequences’ alignments and their analyses were supported by Clustal Omega (www.ebi.ac.uk/Tools/msa/clustalo), Ensembl (http://www.ensembl.org/Homo_sapiens) , and COSMIC (https://cancer.sanger.ac.uk/cosmic) websites .
In silico TERTpmut functional analysis: JASPAR tool
This bioinformatic tool estimates the binding affinity and the number of TFs binding sites for the input sequence provided in FASTA format. A relative threshold score of 80% and Δ relative score ≥ 0.05 (mutant’s relative score—wildtype’s relative score) were chosen to define the statistically significant changes induced by TERTpmut, as previously reported . The JASPAR CORE predicted the effects of the four different TERTpmut that we detected in our patients, i.e. the two new TERTpdup, the TERTp-124, and the TERTp-146, on TFs binding capacity (JASPAR CORE Collection 2020; http://jaspar.genereg.net, 8th version [8, 13]). JASPAR was also used to analyze two TERTpdup, which have been previously reported in a case of MDS (c.1-110_1-101dup) and in a case of thyroid cancer (c.1-104_1-83dup) [21, 23]. According to JASPAR data, we used the Venn diagram to plot TFs for which a significant enhanced probability of binding capacity, or an increase of the number of binding sites, was predicted (http://bioinformatics.psb.ugent.be/webtools/Venn/).
In vitro TERTpmut functional study: luciferase assay
To study the effect of TERTpmut on the expression of TERT, a luciferase assay was done for the TERTpdup detected in case UPN#171, the TERTp-146 (UPN#205), and the TERTp-124 (UPN#216). The TERTdup of case UPN#131 could not be studied due to lack of material. A TERTp wildtype (TERTpwt) construct, already available in the laboratory, was also used as reference (Additional file 2: Table S2) . TERT core promoter (310 bp) was amplified with specific primers reported in Table S3 (Additional file 3: Table S3), introducing cleavage sites for BglII (forward) and HindIII (reverse) restriction enzymes. Then, TERTpmut constructs were inserted in pGEM-T easy plasmid (Promega, Madison WI, USA) and cloned in Electromax DH10BT1 cells (Invitrogen, Milan, Italy) to increase the amount of mutant DNA. Finally, the inserts were subcloned in pGL4.10[luc2] vectors (Promega, Madison WI, USA) upstream of LUC2 gene, encoding for luciferase enzyme of Photinus Pyralis and resequenced. An empty pGL4.10[luc2] vector was also used as negative control. Luciferase assay was performed using the GBM U87-MG cell line, maintained in Dulbecco’s Modified Eagle Medium (Thermo Fisher Scientific, Monza, Italy) with 10% fetal bovine serum, and 0.5% streptomycin/penicillin at 37 °C/5% CO2. U87-MG cells were seeded in a 6-multiwell plate (3 × 105 cells/ml), co-trasfected with 3 µg of modified pGL4.10[luc2] plasmids and with 1:10 of pGL4.74[hRluc/TK], a vector containing the luciferase gene of Renilla Reniformis, by Viafect Transfection Reagent (Promega Madison WI, USA). After 24-h incubation, cells were lysed and fluorescence emission was assessed using Dual-Glo Luciferase assay kit (Promega) following manufacturer’s instructions. All experiments were performed in triplicate, in three independent experiments.
New somatic TERT promoter variants
TERTpmut were detected in 239/301 cases (79.4%), including 14/15 ODG (93%), 12/32 DA/AA (37.5%), and 213/251 GBM (84.8%) (Additional file 4: Table S4). In GBM (= 213) and DA/AA (= 12), TERTpmut were prevalent in IDHwt cases (209/241 GBM IDHwt vs 4/10 GBM IDHmut; 10/12 DA/AA IDHwt vs 2/20 DA/AA IDHmut) (Chi square, P < 0.001) (Additional file 5: Table S5). Thus, in agreement with the diagnostic criteria recommended by the cIMPACT-NOW (Update 3), the 10 DA/AA IDHwt with TERTpmut were referred to as “diffuse astrocytic glioma, IDH-wildtype, with molecular features of glioblastoma, WHO grade IV” .
In GBM TERTpmut there was a significant enrichment of cases harbouring EGFR amplification (46% vs 17%) (Chi square, P = 0.001) and/or monosomy 10/PTEN deletions (84% vs 37.5%) (Chi square, P < 0.0001). Likewise, EGFR amplification or gain of whole chromosome 7 in combination with monosomy 10, occurred in 6/10 (60%) of TERTpmut DA/AA IDHwt.
The most common variant, TERTp-124 was detected in 172 cases while the TERTp-146 was found in 65 cases. TERTpmut were mutually exclusive, heterozygous, and equally distributed among the different histological subtypes (Additional file 5: Table S5). Besides the TERTp-124 and TERTp-146, we uncovered two new TERTp variants in two cases of GBM IDHwt (UPN#131 and UPN#171). These novel TERTpmut consisted of a 22 nucleotide tandem duplication, occurring in a genomic region starting at 100 and 110 bp, from the ATG starting site, i.e. c.1-100_1-79dup (TERTp-100-79), in case UPN#131, and c.1-110_1-89dup (TERTp-110-89), in case UPN#171 (Fig. 2a, b) (www.ncbi.nlm.nih.gov/gene, www.ensembl.org/Homo_sapiens, cancer.sanger.ac.uk/cosmic) [5, 7, 20]. They shared a region of duplication of 12 nucleotides, from 1–100 to 1–89 nucleotides from the ATG start site. The absence of TERTp-100-79 in the PB DNA, demonstrated the somatic origin of this variant in case UPN#131.
In silico analysis predicts TERTpmut effects
In silico analysis predicted that both TERTpdup created new binding sites, i.e. 119 for TERTp-100-79 and 108 for TERTp-110-89, which were respectively recognized by 65 and 53 TFs. Instead, TERTp-124 and TERTp-146 were predicted to increase the binding affinity for 40 and 43 sites, and to enhance the probability of binding for 28 and 29 TFs, respectively (Additional file 6: Table S6). Although all TERTpmut affected the binding sites for diverse families of TFs, the ETS group emerged as one of the most frequently involved: 18/65 (28%) in TERTp-100-79, 18/53 (34%) for TERTp-110-89, 23/28 (82%) in TERTp-124, and 25/29 (86%) in TERTp-146, (Fig. 2c, Additional file 7: Table S7). Other recurrently involved TFs in TERTpdup variants were the Specificity Protein/Krüppel-Like Factor (Sp/KLF) family, i.e. 19/65 (29%) in TERTp-100-79 and 16/53 (30%) in TERTp-110-89, and the More than 3 adjacent zinc finger factors family (12/65 in TERTp-100-79 and 7/53 TERTp-110-89) (Additional file 7: Table S7).
The Venn diagram showed a close inter-relationship between all TERTp mutations. Namely, all TERTp mutations shared an increase of the binding affinity, or the number of binding motifs, for 19 common TFs (Fig. 3a), including 18 ETS members (ETS1, ETS2, ERG, ELK1, ETV6, FLI1, ELK4, SPIB, ELF1, ELF3, ETV4, ETV1, FEV, EHF, ETV5, ELF5, SPI1, and GABPA) and TEAD1 (Fig. 3a; Additional file 8: Table S8). The Venn diagram also showed that the new TERTpdup were characterized by the exclusive involvement of 30 common TFs. Specifically, there were 16 Sp/KLF members, i.e. KLF2, KLF3, KLF4, KLF5, KLF10, KLF11, KLF14, KLF15, KLF16, SP1, SP2, SP3, SP4, SP8, SP9, and EGR1, (Fig. 3a, Additional file 8: Table S8) and 14 TFs that belong to 9 different families (Fig. 3a, Additional files 7 and 8: Tables S7 and S8). Matching our TERTpdup with the two cases of TERTpdup previously reported (Additional files 9 and 10: Tables S9 and S10) [21, 23], JASPAR predicted that all variants determined an increase of binding sites for 21 common TFs, and confirmed that the Sp/KLF family was the most frequently involved (14/21) (Fig. 3b, Additional file 11: Table S11).
In vitro analysis confirms the increasing of TERT transcriptional activity induced by its promoter mutations
In vitro luciferase assay was carried out to evaluate whether the new TERTp-110-89 variant induced an increase of TERT transcriptional activity, enhancing its expression, similarly to TERTp-124 and TERTp-146 [12, 21]. In Table S12 (Additional file 12: Table S12) we reported raw data referred to the fluorescence emission values, expressed in Relative Luciferase Activity (RLA), of both Photinus Pyralis and Renilla Reniformis luciferase enzymes, for all samples. Our experiments demonstrated that all three variants caused a significant increase of TERT transcription by 2.3-2.5 fold than wildtype (TERTp-110-89 vs TERTpwt: P < 0,0001; TERTp-124 vs TERTpwt: P < 0,0315; TERTp-146 vs TERTpwt: P < 0,0001; Mann–Whitney U test) (Fig. 4). On the other hand, no differences on the levels of TERT expression were present between the diverse TERTp variants, indicating they may all behave as gain-of-function mutations, likely exerting the same consequences on TERT transcription.
Abnormal genomic events that alter telomere elongation are common in gliomas. Particularly, mutually exclusive mutations affect the TERT or the ATRX chromatin remodeler (ATRX) genes, a critical regulator of telomere homeostasis by chromatin remodeling .
Our studies, on a cohort of 301 patients, confirmed previous data on the incidence and distribution of TERTpmut in diverse subtypes of CNS tumors. As expected, we found that TERTpmut were highly recurrent in ODG and GBM, and less frequent in DA/AA (Additional file 4: Table S4). TERTpmut were significantly enriched in GBM IDHwt cases (83%) (Chi square, P < 0.001) (Additional file 55: Table S5), where they mainly occurred together with EGFR amplification (Chi square, P = 0.001) and/or monosomy 10/PTEN deletions (Chi square, P < 0.0001). Similarly, in DA/AA, TERTpmut were highly recurrent in IDHwt cases, thus allowing the reclassification of 83% of these subgroup of astrocytomas as “diffuse astrocytic glioma, IDH-wildtype, with molecular features of glioblastoma, WHO grade IV” .
Besides the two known TERTp-124 and TERTp-146 variants, we uncovered two new TERTp variants in two cases of GBM IDHwt (UPN#131 and UPN#171). These novel TERTpmut consisted of a 22 nucleotide tandem duplication, sharing a duplicated region of 12 nucleotides, from 1–100 to 1–89, from the ATG start site. Hitherto, somatic TERTpdup has been reported in three human tumors. The first one, a duplication of 41 nucleotides in the TERT core promoter, was detected in a case of ODG . Afterwards, TERTpdup were found in a case of myelodysplastic syndrome (MDS) (c.1-110_1-101dup) and in a case of papillary thyroid carcinoma (c.1-104_1-83dup) [21, 23]. Published TERTpdup as well as our cases, are located in the same core promoter region, that span 1-110/1-79 bp from the ATG start site. Furthermore, they are all located downstream TERTp-124 and TERTp-146, i.e. at 13–23 nucleotides from TERTp-124 and 35-45 nucleotides from TERTp-146, in a region that contains the binding sites for the TFs modulating TERT transcription. Interestingly, in silico analysis predicted these new TERTdup affect the transcriptional regulation of the gene through the creation of new binding sites for TFs that mainly belong to the ETS family (Fig. 2c, Additional file 7: Table S7). Likewise, an increased number of binding sites or an enhanced affinity for the ETS TFs, has been previously reported in a thyroid cancer harbouring a TERTp c.1-104_1-83dup variant, and in cases bearing TERTp-124 or TERTp-146 mutations [3, 10, 23]. Bioinformatic analyses were consistent with the luciferase data showing a significant increase of TERT expression in cells transfected with the new TERTp-110-89 variant as well as with the two recurrent TERTpmut.
Then, we sought to assess the possible inter-relationship between the four diverse TERTp mutations using the Venn diagram (Fig. 3a). All four TERTp variants were predicted to share an increase binding capacity for 18 ETS members (Fig. 3a; Additional file 8: Table S8), which included GABPA, a putative oncogene in GBM. Namely, in vitro studies on GBM cell lines have demonstrated that this transcription factor is needful in mediating the transcriptional reactivation of TERT dependent from TERTp-124 or TERTp-146 [3, 10, 19]. Besides ETS TFs, all TERTp variants affected the binding capacity for TEAD1, a protein that belongs to TEF-1-related factors family, and that has been demonstrated to act as a putative oncogene in GBM, favoring cell infiltration in vitro/in vivo models .
Although TERTp-124 and TERTp-146, and the new TERTp-100-79 and TERTp-110-89 variants, shared the same effects on the binding capacity for ETS members, the latters were characterized by the exclusive involvement of 30 TFs, mainly belonging to Sp/KLF family (Fig. 3a, Additional files 7 and 8: Tables S7 and S8). Sp/KLF TFs are involved in a plethora of cellular processes ranging from proliferation and differentiation, pluripotency and apoptosis, in normal and tumoral tissues .
Altogether these data support the hypothesis that the recruitment of ETS family TFs plays a pivotal role in mediating the reactivation of TERT transcription in human tumors bearing different types of TERTpmut. However, they also indicate that slight differences mark TERTpdup variants, whose activities appear to be also dependent from Krüppel-related factors. Indeed, among the 21 TFs shared by all TERTpdup (Fig. 3b), 14 belonged to Sp/KLF family (67%) as reported in Tables S10 and S11 (Additional files: 10 and 11). Hence, the precise definition of mutation-specific profiles would strengthen the definition of TERT-dependent oncogenesis mechanisms.
Our study contributes to enrich the spectrum of recurrent somatic TERTpdup variants reporting, for the first time, two new gain-of-function mutations, i.e. TERTp-100-79 and TERTp-110-89, in 0.8% of GBM IDHwt cases. These new mutations can be reliably detected by diagnostic assays used to investigate hotspot TERTp-124 and TERTp-146. Although the assessment of TERTp mutational status is not an essential diagnostic criterion, it can be a relevant information to assist histological diagnosis . As a matter of fact, the status of TERTp, together with IDH mutations and 1p/19q co-deletion, classify gliomas in 5 distinct subcategories, i.e. triple negative, triple positive, cases with IDH/TERT mutations, and cases with a unique mutation (either IDH or TERT), that are typified by unique demographic, clinical and biological characteristics . Moreover, TERTpmut has been proposed as one of the most relevant molecular marker to stratify DA/AA IDHwt . Thus, we consider that molecular testing of TERTp mutations should be included in the clinical work-up of GBM and DA/AA in order to provide a precise diagnosis: prospective multicentric studies, on large cohort of patients, will clarify the value of TERTp mutations as prognostic marker.
Availability of data and materials
All data generated or analyzed during this study are included in this published article [and in its supplementary information files].
Telomerase Reverse Transcriptase
TERT promoter mutation
TERT promoter duplication
c.1-124 TERT promoter mutation
c.1-146 TERT promoter mutation
E-twenty-six transcription factor
central nervous system
diffuse astrocytoma IDH-wildtype
anaplastic astrocytoma IDH-wildtype
diffuse astrocytoma IDH-mutant
anaplastic astrocytoma IDH-mutant
Specificity Protein/Krüppel-Like Factor
relative luciferase activity
ATRX chromatin remodeler
Allory Y, Beukers W, Sagrera A, Flández M, Marqués M, Márquez M et al (2014) Telomerase Reverse Transcriptase promoter mutations in bladder cancer: high frequency across stages, detection in urine, and lack of association with outcome. Eur Urol 65:360–366
Brat DJ, Aldape K, Colman H, Holland EC, Louis DN, Jenkins RB et al (2018) cIMPACT-NOW update 3: recommended diagnostic criteria for “Diffuse astrocytic glioma, IDH-wildtype, with molecular features of glioblastoma, WHO grade IV”. Acta Neuropathol 136:805–810
Ensembl Database-Homo Sapiens (2020) European Molecular Biology Laboratory’s European Bioinformatics Institute, Cambridge UK. http://www.ensembl.org/Homo_sapiens. Accessed 30 May 2020
Fornes O, Castro-Mondragon JA, Khan A, van der Lee R, Zhang X, Richmond PA et al (2020) JASPAR 2020: update of the open-access database of transcription factor binding profiles. Nucleic Acids Res 48:D87–D92. https://doi.org/10.1093/nar/gkz1001
JASPAR CORE Collection Database (2020) University of Copenhagen, Centre for Molecular Medicine and Therapeutics, London Institute of Medical Sciences, Centre for Molecular Medicine Norway. http://jaspar.genereg.net. Accessed 30 May 2020
Killela PJ, Reitman ZJ, Jiao Y, Bettegowda C, Agrawal N, Diaz LA Jr et al (2013) TERT promoter mutations occur frequently in gliomas and a subset of tumors derived from cells with low rates of self-renewal. Proc Natl Acad Sci USA 110:6021–6026
Labussière M, Di Stefano AL, Gleize V, Boisselier B, Giry M, Mangesius S et al (2014) TERT promoter mutations in gliomas, genetic associations and clinico-pathological correlations. Br J Cancer 111:2024–2032
Louis DN, Perry A, Reifenberger G, von Deimling A, Figarella-Branger D, Cavenee WK et al (2016) The 2016 World Health Organization Classification of tumors of the central nervous system: a summary. Acta Neuropathol 131:803–820
National Center for Biotechnology Information Database-Gene (2019) U.S. National Library of Medicine, Rockville Pike. http://www.ncbi.nlm.nih.gov/gene. Accessed 30 May 2020
Nofrini V, Matteucci C, Pellanera F, Gorello P, Di Giacomo D, Lema Fernandez AG et al (2020) Activating somatic and germline TERT promoter variants in myeloid malignancies. Leukemia [Online ahead of print]
Nonoguchi N, Ohta T, Oh JE, Kim YH, Kleihues P, Ohgaki H (2013) TERT promoter mutations in primary and secondary glioblastomas. Acta Neuropathol 126:931–937
Pekmezci M, Rice T, Molinaro AM, Walsh KM, Decker PA, Hansen H et al (2017) Adult infiltrating gliomas with WHO 2016 integrated diagnosis: additional prognostic roles of ATRX and TERT. Acta Neuropathol 133:1001–1016
Zhang ZY, Chan AK, Ding XJ, Qin ZY, Hong CS, Chen LC et al (2015) TERT promoter mutations contribute to IDH mutations in predicting differential responses to adjuvant therapies in WHO grade II and III diffuse gliomas. Oncotarget 6:24871–24883
The project was supported by Comitato per la vita “Daniele Chianelli”, Perugia, Italy; Sergio Luciani Association, Fabriano, Italy; and Fondazione Cassa di Risparmio Perugia, Italy (Grant numbers: 2018.0418.021 to RLS).
Cristina Mecucci, Roberta La Starza have equally contributed to this work
Authors and Affiliations
Molecular Medicine Laboratory, Centro di Ricerche Emato-Oncologiche (C.R.E.O.), S. Maria della Misericordia Hospital, University of Perugia, P.le Menghini 9, 06132, Perugia, Italy
Tiziana Pierini, Carlotta Nardelli, Anair Graciela Lema Fernandez, Valentina Pierini, Fabrizia Pellanera, Valeria Nofrini, Paolo Gorello, Martina Moretti, Silvia Arniani, Cristina Mecucci & Roberta La Starza
Hematology and Center of Bone Marrow Transplants, Medicine and Surgery Department, University and Hospital of Parma, Via Gramsci 14, 43126, Parma, Italy
Diagnostic Cytology and Histology Unit, S. Maria della Misericordia Hospital, P.le Giorgio Menghini 8/9, 06132, Perugia, Italy
Division of Radiotherapy, S. Maria della Misericordia Hospital, P.le Giorgio Menghini 8/9, 06132, Perugia, Italy
Medical Oncology, S. Maria della Misericordia Hospital, P.le Giorgio Menghini 8/9, 06132, Perugia, Italy
Giulio Metro & Carmen Molica
Division of Neurosurgery, S. Maria della Misericordia Hospital, P.le Giorgio Menghini 8/9, 06132, Perugia, Italy
Corrado Castrioto & Rodolfo Corinaldesi
Pathology Unit, S. Maria Hospital, V. Tristano di Joannuccio, 05100, Terni, Italy
TP, RLS conceived the study, planned the experiments, and wrote the paper; TP carried out and evaluated mutational analysis and in vitro functional studies; CN made in silico analysis; AGLF, contributed in the analysis of in vitro luciferase assay; MM and SA performed DNA extraction and FISH experiments; FP, VN and PG performed sequencing analysis; PG, SA, and MEL provided the diagnosis and the tissue sections for molecular-cytogenetic studies; CC and RC, ML, GM and CM provided all clinical data; VP, GR and CM were involved in drafting the manuscript. All the authors read and approved the final manuscript.
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.