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Loss of USP18 in microglia induces white matter pathology

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Ubiquitin specific protease 18 (USP18) is a major negative regulator of the type 1 interferon (IFN) pathway. In a recent publication we showed that USP18 is a key molecule imposing microglial quiescence specifically in the white matter [7]. USP18 is a negative regulator of the type 1 interferon (IFN) pathway [9]. Microglia lacking Usp18 exhibited constitutive activation of type I IFN signaling pathways resulting in markedly elevated expression of multiple interferon-stimulated genes (ISGs) [7]. Additionally, Usp18-deficient brains exhibited clusters of microglia in the white matter that strongly resembled the neuropathological state in several human microgliopathies. Human diseases in which microgliopathies play a primary role comprise Nasu-Hakola disease [14], hereditary diffuse leukoencephalopathy with spheroids (HDLS) [15] and Pseudo-TORCH syndrome (PTS), including Aicardi–Goutières syndrome [12]. One might speculate that activated microglia in the white matter induce white matter abnormalities with functional consequences. However, there were no cells which had taken up myelin in young adult mice as seen by luxol fast blue–PAS (LFB–PAS) histology (unpublished data). Myelin uptake by other cells, like macrophages, would have been indicative of myelin damage. That is why we now characterized conditional myeloid-specific Usp18 deficient mice in more detail.

We know that Usp18 transcripts are highly expressed in unstimulated white matter microglia with only negligible expression levels in other CNS cells [7]. In a previous study, we have confirmed by PCR analysis that Cx3cr1Cre:Usp18fl/fl mice have an Usp18 deletion in microglia but not in neuroectodermal cells of the CNS. These mice displayed a significant increase of Iba1+ microglia cell numbers in several white matter regions including the corpus callosum as young adult mice [7]. This microgliosis persisted with increasing age and was detectable even in 4- and 8-month old mice (Fig. 1a, b). Usp18-deficient microglia exhibit constitutive expression of IFN target genes and fail to downregulate IFN-induced genes because the termination of type I IFN signaling is severely impaired. This became evident by the increase in ISG15 positive cells in the corpus callosum (Fig. 1a, b) and the elevated phosphorylation of STAT1 in Usp18-deficient microglia when compared to Usp18fl/fl mice (Fig. 1c). We next investigated animals at later ages than before by immunostainings against lysosome-associated membrane protein-2 (LAMP2) as a marker of phagocytosis [4]. We found increased LAMP2 positive signals in microglia, which were localized in the corpus callosum of Cx3cr1Cre:Usp18fl/fl mice at an age of 4 months (Fig. 2a, b) and 8 months (Fig. 2c, d). To analyze white matter integrity, we performed high-resolution (11.7 T) diffusion tensor imaging (DTI). We calculated the fractional anisotropy (FA) values, permitting an exploration of the orientation coherence of axons in this fiber bundle. We found that the FA values were reduced in the corpus callosum, the internal and external capsule of Cx3cr1Cre:Usp18fl/fl mice (cf. Usp18fl/fl controls), suggesting diminished structural integrity of the white matter in 4- and 8-month old animals (Fig. 2e). Additionally, we found increased numbers of cells that had incorporated myelin and thereby indicate damage to the myelin sheaths (Fig. 2f). Together, these findings point to a reduction in myelination or even to a loss of fibers in Cx3cr1Cre:Usp18fl/fl mice [2, 17].

Fig. 1

Microgliosis in corpus callosum of Cx3cr1Cre:Usp18fl/fl mice. a, b Histology of corpus callosum in the cerebrum of adult Usp18fl/fl and Cx3cr1Cre:Usp18fl/fl mice at 4 (a) and 8 months of age (b). Primary antibodies against Iba1 and ISG15 were used. To quantify the number of Iba1+ or ISG15+ cells at least six mice per genotype and 5 sections per mouse from two independent experiments were counted. Quantification of cells is shown next to the respective histological images. Significant differences were determined by an unpaired t-test or Mann-Whitney U-test and marked with asterisks (***P < 0.001 versus control littermates). Bars represent means ± S.E.M. Scale bars = 25 μm, 50 μm, 100 μm. c Immunohistochemistry for phosphorylated STAT1 (pSTAT1, red), CD11b (green) and DAPI (blue) in the corpus callosum of 8- month old Usp18fl/fl and Cx3cr1Cre:Usp18fl/fl mice. Scale bar: 20 μm. Quantification of pSTAT1+CD11b+ cells is shown next to the respective histological images. Each symbol represents one mouse. Error bars represent S.E.M. Significant differences are determined by an unpaired t-test and marked with asterisks (***P < 0.001)

Fig. 2

USP18-deficient microglia reduces structural integrity in corpus callosum. a Immunofluorescent histochemistry for Iba1 (red), Lamp2 (green) and DAPI (blue) in the corpus callosum of 4 months and 8 months (c) old Usp18fl/fl and Cx3cr1Cre:Usp18fl/fl mice. Scale bar: 20 μm. Quantification of Iba1+ and percentage of Iba1+Lamp2+ cells is shown next to the respective histological images (b, d). Each symbol represents on mouse. Error bars represent s.e.m. Significant differences are determined by an unpaired t-test and marked with asterisks (**P < 0.01, ***P < 0.001). e DTI was performed on 4 and 8 months old Usp18fl/fl and Cx3cr1Cre:Usp18fl/fl mice to measure the FA of the corpus callosum. Tensor images were collectively acquired in several horizontal planes from + 2.0 to − 4.0 mm from the bregma, with an interplane distance of 0.5 mm (Usp18fl/fl, n = 6; Cx3cr1Cre:Usp18fl/fl, n = 4). Heat maps of the FA values showing the average (of all Usp18fl/fl and Cx3cr1Cre:Usp18fl/fl animals) of one plane from each group (from anterior to posterior). Warm colors indicate fiber tracts with strong diffusion coherence. For both age groups the FA values were significantly reduced in Cx3cr1Cre:Usp18fl/fl mice in comparison to Usp18fl/fl mice. Approximate locations of the regions of interest (ROIs) are indicated. Data are means ± SEM. (*P < 0.05, **P < 0.01, ***P < 0.001, n.s. = non-significant). Statistical significance was determined using multiple t tests corrected for multiple comparisons using the Holm-Sidak method with a = 0.05. f Histological analysis by luxol fast blue–PAS (LFB–PAS) in 8-month-old Usp18fl/flmice and Cx3cr1Cre:Usp18fl/fl littermates. Representative of n = 6 Usp18fl/fl and n = 7 Cx3cr1Cre:Usp18fl/fl mice. Circles represent individual mice. Unpaired two-tailed t-test

Deterioration of white matter tracts, affecting brain structural (SC) and functional connectivity (FC) is often paralleled by behavioral declines [3, 6, 8]. We therefore tested Cx3cr1Cre:Usp18fl/fl mice and Usp18fl/fl littermate controls in different behavioral paradigms. While mice lacking Usp18 in microglia performed normal in the odor avoidance test at 4 months of age (Fig. 3a), 8-month old Cx3cr1Cre:Usp18fl/fl mice showed severely impaired olfaction (Fig. 3d). Similarly, learning and recognition memory was fully intact at 4 months of age (Fig. 3b) but decreased when Cx3cr1Cre:Usp18fl/fl mice were 8-month old compared to age-matched Usp18fl/fl control mice (Fig. 3e). Rotarod performance, which measures motor coordination and motor learning, was also significantly impaired in 8-month old Cx3cr1Cre:Usp18fl/fl mice (Fig. 3f) with no deficits in 4 months old mice (Fig. 3c). In addition to the indicated mouse model we investigated brainstem tissue samples from three PTS patients with loss-of-function recessive mutations of USP18 [12]. Immunohistochemistry showed increased STAT1 phosphorylation in microglia of PTS patients when compared to age-matched control tissue (Fig. 4a). In patients’ material there were also more microglial cells, which engulfed cells positive for Nogo-A (Fig. 4b), which represents an oligodendroglial marker [11].

Fig. 3

Gradual behavioral impairment in Cx3cr1Cre:Usp18fl/fl mice. a, d Olfactory avoidance test. The time animals spent away from the odorant zone was recorded. b, e Novel object recognition. The time a mouse spent investigating a familiar (f) or novel (N) object was recorded. The object interaction ratio was defined as the difference in exploration time for the novel object divided by the exploration time for the familiar object. c, f) Rotarod. Graphed is the latency to fall off the rod during accelerating speed (4–40 r.p.m). For all three tests, performance of Usp18fl/fl and Cx3cr1Cre:Usp18fl/fl animals was compared when they had reached 4 and 8 months of age. Asterisks indicate significant differences (*P < 0.05, **P < 0.01 and ***P < 0.001, n.s. = not significant)

Fig. 4

Microgliosis in white matter of Pseudo-TORCH patients. a Histology of white matter in Pseudo-TORCH patients (n = 3) and age-matched controls (n = 3) (b). Primary antibodies were used against Iba1, pStat1 and Nogo-A. Quantification of cells is shown next to the respective histological images. Significant differences were determined by an unpaired t-test or Mann-Whitney U-test and marked with asterisks (***P < 0.001 versus controls). Bars represent means ± s.e.m. Scale bars = 50 μm, 100 μm

The data presented here indicate that in myeloid-specific Usp18 knockout animals, microglia in the white matter were not only activated, but also caused advancing damage to this structure with subsequent behavioral impairment of the animals. USP18-deficiency in humans belongs to a group of genetic disorders that are collectively termed type I interferonopathies. These disorders are first characterized by the persistent up-regulation of type I interferon signaling [16]. There have been at least seven possible cellular mechanisms described, which result in sustained activation of interferon signaling [16]. One of them, PTS, is a group of not so well-defined genetic diseases, which can originate from USP18 deficiency. We found that microglia in PTS patients displayed not only enhanced type I IFN signaling, but also close contact to oligodendroglia. A direct interaction might indicate that activated microglia, as suggested by their focally elevated cell density together with altered morphological properties inflict damage to oligodendroglia. This strongly resembles the white matter damage observed in Cx3cr1Cre:Usp18fl/fl mice. Type I interferon can be regarded as a neurotoxin if its levels are not tightly controlled. Accordingly, experiments undertaken in mice demonstrate that overexpression of interferon in the CNS results in neuropathology reminiscent of that seen in certain type I interferonopathies [1, 10]. In the case of PTS, but also in the case of type I IFN overexpression, damage to the white matter seems to be prevalent [5, 12]. It is still unclear what the type I IFN source is in the context of interferonopathies. Likewise it is enigmatic which signals are responsible for microglia activation in the white matter. The escalating spiral of white matter damage might be initiated by type I IFN that is induced in microglia via stimulator of interferon genes (STING), and this IFN likely influences the microglial phenotype in an autocrine and paracrine fashion [13].

The white matter specificity of the USP18 effect on microglia is of particular interest and further developments in this area may have implications for an entire range of neurological disorders in which there is a preponderance of white matter pathology.

Availability of data and materials

The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.



Diffusion tensor imaging




Interferon-stimulated gene


Magnetic resonance imaging


Novel Object Recognition


Pseudo-TORCH syndrome


Room temperature


Signal transducer and activator of transcription 1


Stimulator of interferon genes


Ubiquitin-specific protease


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The authors are thankful to Margarethe Ditter for excellent technical assistance.


TB was supported by the DFG (BL 1153/1–2). TB and MP are supported by the DFG (SFB/TRR167 “NeuroMac”).

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TB, KPK, MECM, GMSM, OS, FLH and MP were responsible for the conception and design of experiments; TBi, LAH and DvE were responsible for MRI measurements; MS, OM, FK, AP and FJMM performed experiments, analysed and interpreted the data, they drafted the paper and revised and edited the final article. All authors read and approved the final manuscript.

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Correspondence to Thomas Blank.

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All animal experiments were approved by the Federal Ministry for Nature, Environment and Consumers’ Protection of the state of Baden-Württemberg (G12/71; G16/107) and were performed in accordance with the respective national, federal and institutional regulations. For patients’ samples written parental consent was obtained. Genetic tests were performed according to The Erasmus University Medical Center’s local ethics board approved protocol MEC-2012387.

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Schwabenland, M., Mossad, O., Peres, A.G. et al. Loss of USP18 in microglia induces white matter pathology. acta neuropathol commun 7, 106 (2019).

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  • Microglia
  • Type I interferon
  • Usp18
  • White matter
  • Phagocytosis
  • Corpus callosum
  • Behavior
  • Magnet resonance spectroscopy
  • Microgliosis