Fig. 2
From: Loss of USP18 in microglia induces white matter pathology
USP18-deficient microglia reduces structural integrity in corpus callosum. a Immunofluorescent histochemistry for Iba1 (red), Lamp2 (green) and DAPI (blue) in the corpus callosum of 4 months and 8 months (c) old Usp18fl/fl and Cx3cr1Cre:Usp18fl/fl mice. Scale bar: 20 μm. Quantification of Iba1+ and percentage of Iba1+Lamp2+ cells is shown next to the respective histological images (b, d). Each symbol represents on mouse. Error bars represent s.e.m. Significant differences are determined by an unpaired t-test and marked with asterisks (**P < 0.01, ***P < 0.001). e DTI was performed on 4 and 8 months old Usp18fl/fl and Cx3cr1Cre:Usp18fl/fl mice to measure the FA of the corpus callosum. Tensor images were collectively acquired in several horizontal planes from + 2.0 to − 4.0 mm from the bregma, with an interplane distance of 0.5 mm (Usp18fl/fl, n = 6; Cx3cr1Cre:Usp18fl/fl, n = 4). Heat maps of the FA values showing the average (of all Usp18fl/fl and Cx3cr1Cre:Usp18fl/fl animals) of one plane from each group (from anterior to posterior). Warm colors indicate fiber tracts with strong diffusion coherence. For both age groups the FA values were significantly reduced in Cx3cr1Cre:Usp18fl/fl mice in comparison to Usp18fl/fl mice. Approximate locations of the regions of interest (ROIs) are indicated. Data are means ± SEM. (*P < 0.05, **P < 0.01, ***P < 0.001, n.s. = non-significant). Statistical significance was determined using multiple t tests corrected for multiple comparisons using the Holm-Sidak method with a = 0.05. f Histological analysis by luxol fast blue–PAS (LFB–PAS) in 8-month-old Usp18fl/flmice and Cx3cr1Cre:Usp18fl/fl littermates. Representative of n = 6 Usp18fl/fl and n = 7 Cx3cr1Cre:Usp18fl/fl mice. Circles represent individual mice. Unpaired two-tailed t-test