Tau phosphorylation regulates the interaction between BIN1’s SH3 domain and Tau’s proline-rich domain
- Yoann Sottejeau†1, 2, 3,
- Alexis Bretteville†1, 2, 3,
- François-Xavier Cantrelle3, 4, 5,
- Nicolas Malmanche1, 2, 3,
- Florie Demiaute1, 2, 3,
- Tiago Mendes1, 2, 3,
- Charlotte Delay1, 2, 3,
- Harmony Alves Dos Alves1, 2, 3,
- Amandine Flaig1, 2, 3,
- Peter Davies6, 9,
- Pierre Dourlen1, 2, 3,
- Bart Dermaut1, 2, 3, 8,
- Jocelyn Laporte7,
- Philippe Amouyel1, 2, 3,
- Guy Lippens3, 4, 5,
- Julien Chapuis1, 2, 3,
- Isabelle Landrieu†3, 4, 5Email author and
- Jean-Charles Lambert†1, 2, 3Email author
© Sottejeau et al. 2015
Received: 10 August 2015
Accepted: 7 September 2015
Published: 23 September 2015
The application of high-throughput genomic approaches has revealed 24 novel risk loci for Alzheimer’s disease (AD). We recently reported that the bridging integrator 1 (BIN1) risk gene is linked to Tau pathology.
We used glutathione S-transferase pull-down assays and nuclear magnetic resonance (NMR) experiments to demonstrate that BIN1 and Tau proteins interact directly and then map the interaction between BIN1’s SH3 domain and Tau’s proline-rich domain (PRD) . Our NMR data showed that Tau phosphorylation at Thr231 weakens the SH3-PRD interaction. Using primary neurons, we found that BIN1-Tau complexes partly co-localize with the actin cytoskeleton; however, these complexes were not observed with Thr231-phosphorylated Tau species.
Our results show that (i) BIN1 and Tau bind through an SH3-PRD interaction and (ii) the interaction is downregulated by phosphorylation of Tau Thr231 (and potentially other residues). Our study sheds new light on regulation of the BIN1/Tau interaction and opens up new avenues for exploring its complex’s role in the pathogenesis of AD.
Alzheimer’s disease (AD) is a progressive, neurodegenerative disorder characterized by (i) the massive loss of neurons in several regions of the brain. There are two distinct types of lesion: intraneuronal neurofibrillary tangles (NFTs, composed of abnormally phosphorylated Tau proteins) and extracellular amyloid deposits (composed of amyloid-β peptide (Aβ)). Mutations in the genes for amyloid precursor protein (APP), presenilin-1 and presenilin-2 are responsible for rare, autosomal-dominant forms of AD. The discovery of these mutations prompted the amyloid cascade hypothesis, which has radically changed our understanding of AD; APP metabolism and Aβ peptide production/degradation are thought to have a key role in the pathogenesis of AD (or at least the rare, familial forms of AD) . However, the validity of this hypothesis in the vast majority of cases of AD (the so-called sporadic form) is subject to debate. Since 2009, the application of high-throughput genomic approaches has led to the characterization of 24 additional genetic risk factors for sporadic AD (following on from the apolipoprotein E (APOE) gene, which was characterized as a major genetic risk factor in 1993) . We recently reported that the bridging integrator 1 (BIN1) gene is the first of these new genetic determinants for sporadic AD with a clear link to Tau pathology and (potentially) neurofibrillary degeneration . We found that BIN1 was upregulated in the brains of AD cases and that a functional variant modulating BIN1 expression was associated with NFT loads. We were also able to show that BIN1 and Tau were directly interacting together in vitro and that human Tau toxicity observed in Drosophila melanogaster was partly suppressed by the silencing of the Drosophila BIN1 ortholog . Taken as a whole, these data suggest that BIN1 interacts with Tau and is involved in Tau pathology. We therefore decided to characterize the BIN1-Tau interaction and the latter’s putative regulatory mechanisms in more detail. In the present work, we used glutathione S-transferase (GST) pull-down and nuclear magnetic resonance (NMR) experiments to show that BIN1’s SH3 domain interacts with Tau’s proline-rich domain (PRD). We found that amino acids [212–231] in Tau are essential for this interaction, and that phosphorylation within this sequence weakens the binding. Lastly, we used a proximity ligation assay (PLA) in primary neuron cultures to show that BIN1-Tau complexes co-localize with the actin cytoskeleton. However BIN1-Tau complexes were not observed in neurons when Tau Thr231 is phosphorylated. Taken as a whole, our results provide a detailed view of the molecular interplay between Tau and BIN1 and show for the first time that Tau phosphorylation weakens the interaction between these two proteins.
Materials and methods
cDNA and plasmids
Tau full-length (FL) 2N4R cDNA in pcDNA four was a kind gift from Luc Buée (INSERM U837, Lille, France). The BIN1 isoform used in the present study corresponds to the longest neuronal isoform 1 and will be denoted as BIN1 FL. For GST pull-down experiments, both Tau FL and Tau sub-fragment cDNA sequences were obtained by PCR with the primers described in Additional file 1, and subcloned into the pGEX-4 T2 vector (General Electric Healthcare Bio-Sciences, Piscataway, NJ, USA) to produce GST-Tau constructs. GST BIN1 FL and GST-BIN1/SH3 were obtained as previously described . BIN1/ΔSH3 cDNA sequence was obtained by PCR from BIN1 FL cDNA (NM_139343.1) in PCMV6-XL5 (Origene, Rockville, MD, USA). For NMR experiments, the BIN1/SH3 domain cDNA was synthesized with optimized codons for recombinant expression in E. coli (Genecust, Dudelange, Luxembourg). The cDNA was subcloned between the NdeI and XhoI restriction sites in pET15b (Novagen, EMD Millipore, Darmstadt, Germany), thus allowing its expression with an N-terminal HisTag under the control of a T7 promoter. Recombinant Tau-F5[165-245] and TauFL were prepared for NMR experiments without a N-terminal tag with a pET15B vector. All cDNAs were checked by sequencing.
Cell cultures and transfection
Human embryonic kidney 293 (HEK293) cells (CRL-1573 from LGC Standards/American Type Culture Collection, Molsheim, France) were cultured in Dulbecco’s modified Eagle’s medium (DMEM)/F12 (1:1) supplemented with 10 % fetal bovine serum, 2 mM glutamine, 20 units/ml penicillin and 20 μg/ml streptomycin (Gibco, LifeTechnologies, Carlsbad, CA, USA) in 5 % CO2 atmosphere and at 37 °C. Transient transfections were performed using Fugene-HD (Promega, Madison, WI, USA) according to the manufacturer’s instructions. Forty-eight hours later, cells were harvested in Tris-buffered saline (100 mM NaCl, 1 mM EDTA, 50 mM Tris–HCl) and centrifuged at 1000 g for 10 min at room temperature. Cell pellets were stored at−80 °C until processing for GST pull-down.
The GST pull-down assay
The GST fusion proteins were expressed in Escherichia coli BL21(DE3) after induction with isopropyl 1-thio-β-D-galactopyranoside. Proteins were extracted from bacterial inclusion bodies by incubation with lysosyme for 1 h, overnight incubation with N-sarkosyl (0.001 %) and Triton X-100 (0.5 %), sonication and then centrifugation at 12,500 g for 30 min. All steps were performed at 4 °C. The GST fusion proteins were immobilized on glutathione-Sepharose beads (Pierce, ThermoFisher Scientific, Rockford, IL USA) according to the manufacturer’s instructions, and then incubated with HEK293 cell lysates for 1 h at room temperature. Beads were washed in Tris buffered saline, centrifuged at 10,500 g for 1 min and processed for SDS-PAGE analysis.
Isotopic labelling and protein purification
Isotopic labelling of Tau and Tau-F5 was performed by growing recombinant BL21 (DE3) in minimal growth medium supplemented with 15N NH4Cl. The first purification step was performed by heating the bacterial protein extract for 15 min at 75 °C. The 15N Tau protein and 15N Tau[165–245] were recovered in the soluble fraction after centrifugation at 15,000 g for 30 min. The 15N Tau protein and 15N Tau-F5 were purified by cation exchange chromatography in 50 mM phosphate buffer pH 6.3, 1 mM EDTA (5 ml Hitrap SP Sepharose FF, General Electric Healthcare, Little Chalfont, United Kingdom). The pooled fractions from the chromatography purification step were transferred to ammonium bicarbonate by desalting on a 15/60 Hiprep desalting column (G25 resin, General Electric Healthcare) and lyophilized. The His-SH3 protein was purified on Ni-NTA resin, according to the manufacturer’s protocol.
Acquisition and analysis of NMR spectra
1 mM d4-TMSP (3-(trimethylsilyl) propionate was used as an internal reference for proton chemical shifts (CSs) (0 ppm). The NMR buffer was 25 mM Tris-d11 pH 6.6, 30 mM NaCl, 2.5 mM EDTA and 1 mM DTT and 5 % D2O. Two-dimensional [1H, 15N] heteronuclear single quantum coherence (HSQC) spectra were recorded at 298 K on a Bruker 900 spectrometer equipped with a triple-resonance cryogenic probe (Bruker, Karlsruhe, Germany). Spectra were processed using Bruker TopSpin software (version 2.1, Bruker, Karlsruhe, Germany), and peaks were picked using Sparky software (version 3, T. D. Goddard and D. G. Kneller, University of California, San Francisco, CA, USA). The delta (δ) CSs of individual amide resonances of Tau-F5 and Tau FL were calculated with the following equation, while taking account of the relative dispersion of the proton and nitrogen CSs: δ(CS) = [((CS1Hbound- CS1Hfree) + 0.2 (CS15Nbound- CS15Nfree)) 2]1/2. The “bound” and “free” subscripts in the equation correspond to the CSs in the SH3-bound protein or the free protein, respectively.
Phosphorylation of Tau protein
The CDK2/CycA3 protein was prepared and Tau was phosphorylated in vitro as previously described . Enzymatic reactions were terminated by heating for 15 min at 75 °C and then centrifuged. The phosphorylation mixture was buffer-exchanged for NMR buffer using centrifugal desalting columns (Zeba Desalting Columns, with a 0.5 ml bed of G25 resin and a 7 kDa cut-off (Thermofisher Scientific, Waltham, MA USA)).
Electrophoresis and Western blots
Samples were resuspended in Lithium Dodecyl Sulfate buffer supplemented with Nupage antioxidant, heated for 10 min at 95 °C, loaded and separated on a 4–12 % acrylamide gel (Nupage, Novex, Life Technologies, Carlsbad, CA, USA) and blotted on nitrocellulose membranes using a BioRad Trans-Blot transfer system kit (BioRad, Hercules, CA, USA) according to the manufacturer’s instructions. Membranes were blocked and probed with antibodies diluted at the concentration indicated in Additional file 2. All antibodies were purchased directly from the provider (described in Additional file 2), except for CP13 , RZ3  and PHF1 . Membranes were incubated with a horseradish-peroxidase-conjugated secondary antibody (Jackson Immunoresearch Laboratories, West Grove, PA, USA), and revealed by chemiluminescence (Luminata ClassicoTM, EMD Millipore) in a BioRad Chemidoc XRS system (BioRad). Immunoblot data were quantified with ImageLab software (BioRad). Coomassie staining was performed with 0.05 % Brilliant Blue G (mass/volume) in 50 % methanol (vol/vol) and 10 % acetic acid (v/v). Destaining was performed with a 25 % methanol (v/v) and 7 % acetic acid (v/v) solution.
Immunofluorescence assays and PLAs
Cultured cells were fixed on glass coverslips with either 4 % paraformaldehyde (EMS, Hatfield, PA, USA) in Phosphate Buffered Saline (PBS) (Life Technologies). Fixed cells were washed and permeabilized for 10 min in PBS supplemented with 0.25 % Triton X-100, and then blocked in PBS supplemented with 2 % bovine serum albumin for 2 h at room temperature. Coverslips were incubated overnight at 4 °C with primary antibodies (as specified in Additional file 2), washed in PBS and incubated for 45 min with Alexa Fluor antibodies diluted at 1/400 from stock (Molecular Probes, Life Technologies). Alexa Fluor 647 phalloidin (Molecular Probes, Life Technologies)was used as per the manufacturer’s instructions to stain the actin cytoskeleton. For PLAs, the initial steps (from fixation to primary antibody incubation) were the same as those described above for immunofluorescence assays. The following steps (i.e., secondary antibody incubation, ligation, amplification and probing) were performed according to the manufacturer’s instructions (Olink Bioscience, Uppsala, Sweden). Images were acquired with a confocal microscope (LSM 710, Zeiss, Oberkochen, Germany) and processed using ZEN 2012 software (Zeiss). Three independent experiments were performed for each condition. The mean intensity per pixel in three different fields was measured using ImageJ software. Imaris software (Bitplane, Zurich, Switzerland) was used for three-dimensional (3D) image processing.
Primary neuron cultures
Mixed cortical and hippocampal primary cultures were obtained from P0 rats, according to previously described procedures . Briefly, hippocampi and cortices were isolated from newborn rats, and neurons were dissociated by trypsin digestion. Neurons were plated on poly-L-lysine-coated coverslips or six-well plates, and were incubated with Minimal Essential Medium (MEM) supplemented with 10 % fetal bovine serum, Glutamax, MEM vitamins and penicillin/streptomycin (Life Technologies), according to the manufacturer’s instructions. After 24 h, neurons were transferred into serum-free Neurobasal-A medium supplemented with B27 (Gibco, Life Technologies), Glutamax and uridine-deoxyfluorouridine for 14 days of in vitro culture. For Western blot analysis, primary neurons were directly harvested in LDS buffer supplemented with Nupage antioxidant (Life Technologies) and processed as described above in the “Electrophoresis and Western blots” section. For immunofluorescence assays, cells were fixed in 4 % paraformaldehyde (EMS) and processed as described above in the “Immunofluorescence assays and PLAs” section.
BIN1’s SH3 domain interacts with Tau’s PRD
In vitro, the BIN1-Tau interaction depends on Tau’s phosphorylation status
As Tau phosphorylation has been previously shown to regulate Tau PRD-SH3 interactions [11, 12], we next looked at whether Tau phosphorylation could interfere with the BIN1-Tau interaction. We acquired NMR data after the in vitro phosphorylation of Tau FL or a Tau fragment (Tau-F5[165–245]) by recombinant kinases (Fig. 2). Firstly, following in vitro incubation with CDK2/CycA3 , we confirmed that phosphorylation levels increased at several major Tau phosphoepitopes such as pT181 (AT270), pS199, pT205, pS202-T205 (AT8, Tau-1) and pT231 (RZ3, AT180), as shown by immunoblots (Fig. 2a) and NMR analysis (see Additional file 5). A comparison of NMR spectra of CDK2/CycA3-phosphorylated Tau-FL and Tau-F5 in the presence or absence of BIN1/SH3 did not reveal any significant CS perturbations. These findings indicated a lack of interaction between BIN1/SH3 domain and in vitro-phosphorylated Tau-F5 fragment or Tau-FL (Fig. 2b, c and Additional file 6, Additional file 7). Additional experiments with either a recombinant ERK kinase (see Additional file 8) or rat brain extract kinases (see Additional file 9) confirmed these findings. Taken as a whole, our results demonstrate that Tau phosphorylation in and around the PRD weakens the BIN1-Tau interaction by precluding SH3-PRD binding.
BIN1-Tau complexes partly co-localized with the actin cytoskeleton network
In conclusion, these results showed that Tau/BIN1 complexes are found at specific locations in primary neurons (as suggested by the dot-like staining) and partly co-localize with the actin cytoskeleton.
Tau’s phosphorylation status influences BIN1-Tau binding in primary neurons
Progress in genetic analysis (notably in genome-wide association studies) has led to the identification of 24 validated risk loci for AD . BIN1 is the first of these risk factors to have been linked to Tau pathology . In the present study, we used biochemical and cellular approaches to further characterize the relationship between Tau and BIN1. Firstly, we found that BIN1’s SH3 domain interacts directly with the Tau PRD. Secondly, we determined that the amino acid sequence [212–231] within the PRD is involved in this interaction. Thirdly, we showed that Tau phosphorylation weakens the Tau-BIN1 interaction both in vitro and in primary neurons. Fourthly, we found that BIN1-Tau complexes exhibit a co-localization with the actin cytoskeleton in primary neurons.
BIN1 belongs to the amphiphysin protein family, the best-known function of which is to sense and generate membrane curvature through its BAR domain. This protein is also a key regulator of biological functions such as endocytosis, membrane recycling and cytoskeleton regulation . Accordingly, BIN1 recruits some partners (such as AP-2 and clathrin) through its CLAP domain and others (such as dynamin and synaptojanin) through its SH3 domain . Tau proteins are microtubule-associated proteins whose main function is to promote the polymerization and stabilization of the cytoskeleton microtubule network . Tau is also involved in cell signaling pathways via the recruitment of kinases (such as Lck, Fyn, Src [16, 17] and phosphatidylinositol 3-kinase) and signaling adaptors (such as Grb2 [11, 18]). These interactions are mediated by Tau’s PRD. In the present work, we extended these results by showing that BIN1-Tau binding occurs through the SH3 domain and the PRD. Given that Tau phosphorylation (strong regulator of Tau function) is deregulated in AD, we sought to determine whether Tau phosphorylation was involved in regulation of the BIN1-Tau interaction. Our data indicate that Tau phosphorylation can indeed modulate the interaction between BIN1 and Tau in vitro. These results are consistent with previous reports of phosphorylation-dependent binding between Tau PRD and SH3 domains in proteins such as phosphatidylinositol 3-kinase, phospholipase C γ1, Grb2, and Src family kinases [11, 18]. Importantly, the results of our NMR and cell biology experiments suggest that phosphorylation at Thr231 (which is within the Tau PRD sequence that interacts with the BIN1 SH3 domain) weakens this interaction. These data are coherent with previous studies showing that Tau mutants mimicking phosphorylation at Thr231/Ser235 (T231D/S235D) bind 8 times less avidly (compared with native Tau) to the SH3 domain of Fyn , whereas a single mutation at Ser235 (S235E) does not affect the binding . In contrast, mimicking phosphorylation at Ser396 and Ser404 (S396D/S404D) did not modify the affinity of 3-repeat Tau isoforms for Fyn/SH3. The latter literature data are consistent with (i) our PLA staining results for pS396-404 Tau and BIN1 and (ii) the fact that 3-repeat Tau isoforms are expressed in primary neuron cultures. Indeed, it has been well established that both 4-repeat and 3-repeat Tau isoforms are expressed in primary neurons after 14 days of in vitro culture . Moreover, Tau proteins with different phosphorylation patterns (generated with various kinases) failed to interact with BIN1 in vitro (Fig. 3 and Additional file 8 and Additional file 9) but all were phosphorylated at Thr231. This strongly suggests that pThr231 is an important regulator of the BIN1/Tau interaction. Indeed, these findings were confirmed by our PLAs, since no interaction was observed with Thr231-phosphorylated Tau species in primary neurons. However, since Tau phosphorylation patterns are known to be associated with different subcellular locations (e.g. low Thr231 phosphorylation at the neuron’s cortical membrane ), one can argue that cell-sorting mechanisms might be responsible for the lack of interaction of BIN1 with pThr231 Tau in neurons. However, given that other sites in pThr231 Tau may also be phosphorylated, we cannot rule out the possibility that the latter also modify the BIN1/Tau interaction. Further experiments will be required to specify the possible contributions of other Tau phosphorylation sites and identify the mechanisms that underlie the regulation of the BIN1-Tau interaction by phosphorylation. Nevertheless, our finding that phosphorylation of Thr231 strongly influences the BIN1-Tau interaction highlights a putative link to the pathogenesis of AD. Indeed, Thr231 hyperphosphorylation occurs in the very early stages of neurofibrillary degeneration . The enzymes that control this phosphorylation (such as Cdk5 , Gsk3-β , PP2A  and PIN1 ) are markedly deregulated during the AD process. These findings suggest that Tau hyperphosphorylation during AD (or at least phosphorylation at Thr231) can interfere with the physiological stoichiometry of BIN1-Tau complexes and may have a particular impact in the early stages of AD. We and others have shown that BIN1 expression is elevated in AD brains  and that BIN1 levels are correlated with the presence of neurofibrillary tangles . Moreover, our previous work demonstrated that Tau toxicity in Drosophila models was suppressed by downregulating the Drosophila BIN1 ortholog . Although our earlier results suggest that BIN1-Tau complexes have toxic effects, we cannot yet clearly define the roles of these complexes in AD or how Tau Thr231 phosphorylation contributes to this pathway during AD pathogenesis. In order to better understand the role of BIN1-Tau complexes, we performed experiments with markers of the various cell compartments involved in fundamental neuronal functions (such as synapses and endocytosis). Our results showed that BIN1-Tau complexes did not co-localize with either pre-synaptic or post-synaptic terminal markers. Moreover, clathrin (a marker of the endocytosis pathway) did not particularly co-localize with BIN1-Tau complexes. These results suggest that BIN1-Tau complexes exert their functions through cellular pathways other than the currently known pathogenic pathways involving Tau at the synapse  or involving BIN1 in endocytosis . Nonetheless, we demonstrated substantial co-localization of BIN1-Tau complexes and the actin cytoskeleton. Therefore, one can postulate that BIN1 and microtubule-associated Tau have a role at the interface between actin and the microtubule network in neurons. Indeed, previous research has found that BIN1 interacts with the actin cytoskeleton  and serves as a membrane anchoring point for microtubules . Further studies will be needed to fully understand the roles of BIN1-Tau complexes in neurons.
Our results show that BIN1’s SH3 domain interacts directly with the Tau PRD, and that Tau phosphorylation (notably at Thr231) weakens this interaction. Taken as a whole, our findings highlight the link between BIN1, regulation of Tau phosphorylation and AD-related cellular pathways. Better knowledge of the interactions between Tau, BIN1 and the cytoskeleton in neurons may open up some exciting avenues for further research.
We thank the BioImaging Center Lille-Nord de France (BICeL) facility (Lille, France) and the TGE RMN THC FR-3050 facility (Gif-sur-Yvette, France). We also thank the Albert Einstein College of Medicine of Yeshiva University (New York, USA) for the kind gift of Tau-phosphorylation-dependent antibodies. This work was funded by the French National Foundation on Alzheimer’s disease and related disorders, the Lille Métropole Communauté Urbaine council, the French government’s LABEX DISTALZ (development of innovative strategies for a transdisciplinary approach to Alzheimer’s disease) and the US Alzheimer’s association (IIRG-06-25487).
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