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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Tau phosphorylation regulates the interaction between BIN1’s SH3 domain and Tau’s proline-rich domain

Fig. 5

Specific Tau phosphorylation patterns for endogenous BIN1-Tau complexes in primary neuron cultures. A PLA (green) combining anti-BIN1 with either anti-phospho Tau pS396-404 a, pS202 c. or pT231 e antibodies. There was no PLA signal when anti-Tau pT231 was combined with anti-BIN1. Conventional immunofluorescence staining for BIN1 (cyan) and phospho-Tau (red) obtained with the antibodies used in the PLAs (pS396-404 b, pS202 d or pT231 f). g. Quantification of PLA fluorescence intensity in three fields and three independent experiments. The graph shows the ratio between the PLA intensity for BIN1/phosphoTau and phosphoTau alone. (n = 3) AU: arbitrary units. The error bars correspond to the SD

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Acta Neuropathologica Communications

ISSN: 2051-5960

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