Fig. 3

BIN1-Tau complexes partly co-localize with the actin cytoskeleton network. a. Immunofluorescence staining of endogenous BIN1 (green) and total Tau (red) in primary neuron cultures. Arrows indicate the co-localization of BIN1 and total Tau staining. b. PLAs (green) were been used to visualize endogenous BIN1-Tau complexes. Co-staining for total Tau (red) was compared with the PLA (BIN1/total Tau) signal. The arrow indicates the PLA signal located at the end of microtubule structures. c. Immunofluorescent staining of endogenous tubulin (tub, in green) and total Tau (red) in primary neuron cultures. d. A PLA (green) for visualizing endogenous complexes between tubulin and total Tau, combined with immunofluorescent staining of total Tau (red). e. A PLA for BIN1/total Tau (green) was combined with actin staining (red) using Alexa-Fluor633 phalloidin. The arrow shows the location of the PLA signal, with actin staining . f. A 3D image showing the proximity of the green (BIN1/total Tau) signal to the actin staining. A 3D video is presented in Additional file 11. Mag: magnification