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Molecular and clinicopathologic characteristics of CNS embryonal tumors with BRD4::LEUTX fusion
Acta Neuropathologica Communications volume 12, Article number: 42 (2024)
Abstract
Central nervous system (CNS) embryonal tumors are a heterogeneous group of high-grade malignancies, and the increasing clinical use of methylation profiling and next-generation sequencing has led to the identification of molecularly distinct subtypes. One proposed tumor type, CNS tumor with BRD4::LEUTX fusion, has been described. As only a few CNS tumors with BRD4::LEUTX fusions have been described, we herein characterize a cohort of 9 such cases (4 new, 5 previously published) to further describe their clinicopathologic and molecular features. We demonstrate that CNS embryonal tumor with BRD4::LEUTX fusion comprises a well-defined methylation class/cluster. We find that patients are young (4 years or younger), with large tumors at variable locations, and frequently with evidence of leptomeningeal/cerebrospinal fluid (CSF) dissemination. Histologically, tumors were highly cellular with high-grade embryonal features. Immunohistochemically, 5/5 cases showed synaptophysin and 4/5 showed OLIG2 expression, thus overlapping with CNS neuroblastoma, FOXR2-activated. DNA copy number profiles were generally flat; however, two tumors had chromosome 1q gains. No recurring genomic changes, besides the presence of the fusion, were found. The LEUTX portion of the fusion transcript was constant in all cases assessed, while the BRD4 portion varied but included a domain with proto-oncogenic activity in all cases. Two patients with clinical follow up available had tumors with excellent response to chemotherapy. Two of our patients were alive without evidence of recurrence or progression after gross total resection and chemotherapy at 16 and 33 months. One patient relapsed, and the last of our four patients died of disease one month after diagnosis. Overall, this case series provides additional evidence for this as a distinct tumor type defined by the presence of a specific fusion as well as a distinct DNA methylation signature. Studies on larger series are required to further characterize these tumors.
Introduction
DNA methylation arrays greatly improve diagnostic precision and reliability, and are highly effective for the discovery of new tumor types [1]. This is particularly true of central nervous system (CNS) embryonal tumors, a tumor class comprising a growing list of subgroups largely refined by epigenetic studies [2]. These tumors are high-grade malignancies composed of primitive neuroepithelial cells, predominantly seen in childhood. The 2021 update to the World Health Organization (WHO) classification of CNS tumors saw the incorporation of several new CNS embryonal tumors subtypes, including: cribriform neuroepithelial tumor; CNS neuroblastoma, FOXR2-activated; and CNS tumor with BCOR internal tandem duplication [3].
Recently, two cases of CNS embryonal tumors harboring a BRD4::LEUTX fusion were described [4, 5]. One of these reports characterized the fusion in a large series of pediatric cancers [5], while the other incorporated detailed clinical and pathological data, including methylome analysis matching to the novel Heidelberg version 12 classifier class “CNS embryonal tumor with BRD4::LEUTX fusion” [4]. Three additional cases also exist in the literature, which while not specifically described as such, were initially presented before the version 12 of the Heidelberg classifier was available to interrogate this provisional tumor type [6,7,8]. In addition to these 5 cases, we describe 4 additional unpublished new cases of CNS tumors with BRD4::LEUTX fusions, thereby expanding the limited characterization available for this tumor type to date.
Methods
Tissue samples and datasets
The use of human subject material was performed in accordance with the World Medical Association Declaration of Helsinki and with the approval of the participating Institutional Review Boards. This study included previously described cases of CNS embryonal tumors with BRD4::LEUTX fusion as well as cases from the Laboratory of Pathology clinical consult service at the National Cancer Institute (NCI) in Bethesda, MD, USA, as well as available methylation array data in the form of raw IDAT files. Referral consult cases were received from the following institutions: Mayo Clinic (Rochester, Minnesota); UCSF Benioff Children’s Hospital (Oakland, California); Children’s Hospital Colorado (Aurora, Colorado); and University of California San Francisco Medical Center (San Francisco, California). Each of the referral consult cases was reviewed for histopathology as part of the diagnostic process by at least two board-certified neuropathologists (PJC, MQ, DP, KA). Results of IHC stains were retrieved from the reports from each center as available.
Nucleic acid extraction and next-generation sequencing
Genomic DNA and RNA was extracted from 5 μm sections of formalin fixed paraffin embedded tissue (FFPE) sections mounted on slides using the QIAamp DNA FFPE Tissue Kit (Qiagen, Hilden, Germany), RNeasy FFPE Tissue Kit (Qiagen), or AllPrep DNA/RNA FFPE Kit (Qiagen). DNA was set aside for methylation analysis as described below. Next-generation sequencing (NGS) was performed using commercial TruSeq RNA Exome panel (Illumina). Exome RNA sequencing libraries were prepared with 100 ng tumor RNA using the Illumina RNA Prep with Enrichment (L) Tagmentation kit (Illumina) with Exome Probe Panel (Illumina). Final enriched libraries are sequenced on NextSeq 550DX or NovaSeq 6000 (Illumina).
Data processing and variant analysis
After sequencing, the FASTQ files were aligned to human reference genome hg19 (GRCh37) using STAR aligner [9] to generate BAM files. The resulting BAM files were used by Arriba tool [10] to predict fusion calls. The filtered fusions (VCF file) are uploaded to the QIAGEN Clinical Insight (QCI; Qiagen) for annotation, classification, and interpretation. All variants were manually reviewed by visualizing the raw sequencing read alignments using the Integrative Genomics Viewer [11].
DNA methylation profiling and initial analysis
Samples were processed as previously described [12]. Briefly, genomic DNA (250 ng as the standard) was bisulfite-converted (EZ DNA Methylation Kit, Zymo Research D5001). When the total amount of DNA available was less than 250 ng, the sample was run as well. Bisulfite-converted FFPE DNA was processed with the Infinium FFPE DNA Restore kit (Illumina, USA) and was assayed on Infinium MethylationEPIC kit (Illumina, USA), according to the Infinium HD FFPE Methylation Assay automated protocol (Illumina, USA). Methylation data was processed using versions 11.b6 and 12.b6 of the DKFZ-Heidelberg classifiers and NCI-Bethesda classifier [6]. Copy number variant (CNV) profiles were inferred using the R “conumee” package (http://bioconductor.org/packages/conumee/) as implemented in the classifier package. Tumor purity was estimated using RF_purify package and LUMP score from methylation profile [13, 14].
Results
Case descriptions
Five previously published cases [4,5,6,7,8] were identified in the following manner. One case presented a dedicated case report for a tumor matching to the methylation class “CNS embryonal tumor with BRD4::LEUTX fusion”, with demonstration of the fusion [4]. A second case had been discovered in a large series of pediatric cancers where the BRD4::LEUTX fusion had been identified in one case of the series [5]. A third case was found in a series of medulloblastoma, where re-evaluation using version 12 of the Heidelberg classifier showed a match to CNS embryonal tumor with BRD4::LEUTX fusion [7]. A fourth case was included as a low-score match to group 3 medulloblastoma in the initial paper describing the Heidelberg version 11 methylation classifier [6], but which in our analysis of the Heidelberg version 12 classifier, matched to CNS embryonal tumor with BRD4::LEUTX fusion. The fifth case was described in a series of FOXR2-activated neuroblastomas, as a potential diagnostic mimic [8]. In addition to these 5 cases, 4 cases were identified in the clinical diagnostic methylation service at the National Institutes for Health. Clinical and molecular features for these 9 cases are summarized in Table 1. All patients for whom age was known were young children (up to 4 years) with large masses; three female, two male, and four of unknown sex. The initial (pre-methylation profiling) diagnosis, when known, suggested a CNS embryonal tumor, either NOS or a CNS neuroblastoma, FOXR2-activated.
Histopathologic and immunohistochemical tumor characterization
Histologic and immunohistochemical features from our cases (#1–4), along with data retrieved from two previously published cases are described here. Histologically, all six of the assessed cases were highly cellular, showing embryonal morphology with diffuse arrangement (Fig. 1 and Additional file 1: Table S1), with a cell-streaming pattern seen in two cases, and Homer–Wright rosettes in one case. In two cases, the borders with brain parenchyma could be histologically assessed, showing sharp tumor circumscription. Neoplastic cells were small with round to oval nuclei, with two cases presenting some more irregularly shaped nuclei. Cytoplasm was scant. Scattered large, bizarre nuclei were seen in three cases. Four cases displayed necrosis. Brisk mitotic activity and apoptosis were seen in all samples. All cases displayed microvascular proliferation, often with marked multilayering of vessel walls. Calcifications were detected in two tumors histologically, and suspected in a third tumor by imaging exams, but could not be confirmed on the small biopsy examined. All tumors expressed synaptophysin (diffusely), and five expressed OLIG2 variably (Fig. 1). GFAP and neurofilament were expressed in a subset of tumor cells in 2/5 cases examined. Interestingly, one tumor had complete loss of H3k27me3 expression in tumor cells while three tumors showed a markedly attenuated expression of H3k27me3 in tumor cells with areas showing clear loss (Fig. 1) and areas with weak expression in a mosaic configuration. In all three cases tested, immunohistochemistry (IHC) was negative for the mutant protein H3 p.K27M. INI1 and BRG1 proteins were expressed in 5/5 tumors and LIN28 was negative in 3/3 tested. EZHIP was negative for 1/1 cases tested. We note a number of CNS tumor types where H3K27me3 expression is described as lost in specific cases, including IDH-mutant gliomas [15]). Results for all IHC performed are listed in Additional file 1: Table S2.
Methylation, NGS, and CNV findings
The DKFZ-Heidelberg classifier version 12.5 (and its subsequent iterations) included a methylation class “CNS embryonal tumour with BRD4:LEUTX fusion” which allowed for identification of tumors that matched to this class. Methylation profiling of our 4 cases, as well as 4 cases culled from the literature matched to this class. Seven of these 8 cases reached the 0.84 confidence threshold in the match, with the 8th case showing a ‘suggestive’ score (0.79) for this class (Table 1). RNA sequencing was performed for the 4 NIH cases and 3/5 outside cases, identifying the presence of the BRD4::LEUTX fusion for each of the 7 cases interrogated. All fusion junctions involved exon 2 of LEUTX, retaining the homeobox domain, while the BRD4 component of the fusions involved exons 11, 13, or 14, maintaining the bromodomain in all cases (Table 1 and Fig. 2). Inspection of the DNA copy number changes did not show recurrent alterations (copy number profiles for the 4 NIH cases are shown in Additional file 2: Fig. S1), and in addition copy number breakpoints at either the BRD4 or LEUTX loci were not a feature of this neoplasm. Gene expression was available for 3 of the 4 cases profiled at the NIH, where the expression of LEUTX and BRD4 was compared to the clinical dataset of > 2700 samples of a variety of tumor types with gene expression data available. In all 3 cases, expression of BRD4, and especially that of LEUTX was high relative to expression of these genes in the broad dataset of tumor types (Fig. 3). The elevated expression of LEUTX seen in these tumors is in line with a prior study that reported a BRD4::LEUTX fusion in a pediatric sarcoma [16]. Inspection of methylation signatures using UMAP for the four NIH cases and 2 of the previously published cases fore which (IDAT) files were available showed that CNS tumors with BRD4::LEUTX fusion cluster together, near but separate from group 3 medulloblastoma (Fig. 4). Lastly, DNA sequencing did not identify any recurrent pathogenic or likely pathogenic mutations in the 3 cases (cases 1–3) that were sequenced on a large (~ 500 gene) panel.
Radiological findings and clinical follow-up
A representative series of images are shown for case #3, showing a large tumor with heterogeneous contrast enhancement, cystic change and mass effect (Fig. 5). With respect to patient follow-up, cases #1 (15-month-old female) and #2 (3-year-old male) were both alive without evidence of recurrence or progression at with near total resection followed by chemotherapy. Case #1 was alive one year post-therapy. Case #3 was a 14-month-old male who underwent a stereotaxic biopsy followed by four cycles of chemotherapy, with marked reduction on the enhancing solid tumor component, and gross total resection four months after the initial diagnosis. Two weeks post-surgery, there were signs of local recurrence and salvage therapy was indicated. Case #4 was a 12-month-old female with hydrocephalus with a heterogeneously enhancing mass with evidence of leptomeningeal spread. The patient underwent a stereotactic biopsy and the tumor showed striking response to chemotherapy with tumor shrinkage and nearly complete resolution of the intracranial leptomeningeal enhancement after chemotherapy (Fig. 6). Of note, cases #1–4 were all described to have been treated in a manner similar to clinical trial NCT00336024, where the chemotherapy regimen included cisplatin, cyclophosphamide, vincristine, and etoposide followed by thiotepa. Case #5 was a 4-year female [4] presenting with a large left parietal mass with multiple smaller supra- and infra- tentorial tumor nodules, as well as signs of subependymal and leptomeningeal dissemination. The patient died of disease shortly after surgery.
Discussion
We report on a series of cases that show fusions involving BRD4 and LEUTX and match to the corresponding DKFZ-Heidelberg class describing this tumor type. Patients with these tumors are young, and the tumors show features of an embryonal neoplasm. These are rare tumors, but important to recognize, as they are genomically and epigenetically distinct from other CNS embryonal neoplasms. The BRD4 gene plays a crucial role in transcriptional regulation and in development, it maintains stem cell pluripotency. Dysregulation of BRD4 has been linked to various cancers, where it contributes to cell proliferation and tumor progression. Known fusions include the BRD4::NUT fusion in NUT midline carcinoma [17,18,19]. The LEUTX gene (also known as Leucine Twenty Homeobox), is a homeobox gene with a role in embryonic development and in regulating early cell differentiation. Fusions in this gene also play a role in cancer, including in primary CNS sarcomas involving CIC::LEUTX fusions [20, 21], and of interest, CIC::LEUTX fusions have recently been described in a novel glioneuronal tumor [22].
Interestingly, all fusions had the same breakpoint in LEUTX (exon 2; Fig. 2), which has previously been shown to lead to increased LEUTX expression, otherwise present almost exclusively in non-neoplastic embryonal tissues [16]. In three cases (#1, #3, and #4) the fusion transcript involving exon 11 of BRD4 was identical to the one reported in an embryonal CNS tumor and a pediatric sarcoma with epithelioid features [16]. The BRD4 portion encoded by this transcript (in the estimated chimeric protein) has the same length and structure of a shorter isoform of the protein (BRD4-S) previously shown to be implicated in breast cancer growth and metastasis, potentially mimicking BRD4-S pro-oncogenic activity [23, 24]. In case #2 and case #5 [4], the BRD4 component of the fusion transcript is longer (Table 1) but still does not include a positive transcription elongation factor b (P-TEFb) interaction domain, and the intrinsically disordered region (IDR) is shorter than in the long BRD4 isoform (BRD4-L); the latter being associated with tumor suppressor activity [25]. Of note, small-molecule BRD4 inhibitors and BRD4 degraders have shown promising results in preliminary studies in hematological and solid malignancies, and may represent a future therapeutic avenue for these aggressive tumors [26].
Despite molecular similarity, morphological and immunohistochemical features do not appear to overlap between BRD4::LEUTX fused CNS tumors and the single case of sarcoma with epithelioid features reported in the literature [16], nor with one case of possible alveolar rhabdomyosarcoma also reported to harbor a BRD4::LEUTX fusion [27]. DNA methylation profiling of these sarcomas would be helpful to determine how epigenetically similar these diseases are to CNS embryonal tumors with the same fusion. Indeed, pathological features can overlap between embryonal tumor subtypes, particularly those with BRD4::LEUTX fusions and FOXR2-activated neuroblastomas; most notably the small poorly differentiated cells, abundant necrosis, and co-expression of immunomarkers synaptophysin and OLIG2. A further overlapping feature with FOXR2-activated neuroblastoma is the gain of chr1q, which was found in two of our cases, and positivity for SOX10 seen in one of two cases tested [7].
Depending on tumor location, other embryonal tumors such as medulloblastoma and pineoblastoma should be considered in the differential diagnosis for this tumor type. Well-formed rosettes were not seen in all cases in our series, but these are not necessarily observed in medulloblastomas and pineoblastomas. OLIG2 positivity would be unexpected in both tumors. Embryonal tumors with multilayered rosettes (ETMRs) which otherwise lack rosettes, a medulloepithelioma component, and neurocytic/neuropil-rich areas could also resemble embryonal tumors with BRD4::LEUTX fusions, but they are generally negative for OLIG2 and positive for LIN28A. The recently described entity “CNS tumor with PLAGL amplification” (particularly PLAGL2 in infants and toddlers) also shows overlapping embryonal features with the tumors in this series [28], nonetheless with a different immune-profile—OLIG2 being mostly negative, and synaptophysin being patchy and weak. Interestingly, reduction of H3k27me3 was detected in three cases, one in the tectal plate/third ventricle, and two located in peduncular/thalamic region, with complete loss of expression reported in one case in the parietal lobe [3]. Nonetheless, these potential mimics of diffuse midline glioma (DMG), K27-altered tumors seem to be more circumscribed, without evidence of diffuse infiltration both radiologically and histologically. Also, most DMG show glial morphology, and most do not exhibit extensive positivity for synaptophysin. Moreover, the loss of H3k27me3 can be observed in different CNS tumors and is caused by various mechanisms, generally leading to loss of transcriptional repression [29].
Our sample size is small, but initial clinical investigation showed that these were clinically aggressive, and the evolution of patient #5, who died of disease progression shortly after surgery, is in line with these features. Nevertheless, two patients who underwent stereotactic biopsies showed tumor reduction with chemotherapy, permitting subsequent gross total resection, though one of these relapsed. Two patients were alive without evidence of recurrence or progression after gross total resection and chemotherapy 16 and 33 months after initial diagnosis, one of them already one year off-therapy, suggesting that at least some of these tumors may benefit from chemotherapy.
Conclusions
In this study, we find that CNS embryonal tumor with BRD4::LEUTX fusion is a provisional tumor type occurring in young children. They comprise a well-defined methylation cluster separate from other embryonal tumors and should therefore be considered in young children with small round cell tumors that do not match any known tumor classes; most tumors are synaptophysin and OLIG2 positive. Some of these tumors may show response to chemotherapy regimens. Of note, BRD4 is a bromodomain protein that plays a key role in epigenetic memory, and bromodomain inhibitors have been suggested as a possible therapeutic strategy in other tumor types. Studies on larger cohorts are required to further characterize these tumors.
Availability of data and materials
Primary data will be provided upon reasonable request.
Abbreviations
- BAM:
-
Binary alignment map
- BRD4::LEUTX :
-
Bromodomain containing 4-leucine twenty homeobox
- CNS:
-
Central nervous system
- CNV:
-
Copy number variant
- CSF:
-
Cerebrospinal fluid
- DKFZ:
-
Deutsches Krebsforschungszentrum
- DMG:
-
Diffuse midline glioma
- ETMR:
-
Embryonal tumor with multilayered rosettes
- FASTQ:
-
FAST quality
- FFPE:
-
Formalin-fixed paraffin-embedded
- FOXR2 :
-
Forkhead box R2
- GFAP:
-
Glial fibrillary acidic protein
- GTR:
-
Gross total resection
- H3k27me3:
-
Tri-methylation measure of lysine 27 on histone 3 protein
- H3K27M:
-
Lysine 27 mutated to methionine in histone 3 gene isoforms
- IDAT:
-
Intensity data
- IDR:
-
Intrinsically disordered region
- IHC:
-
Immunohistochemistry
- NGS:
-
Next-generation sequencing
- OLIG2:
-
Oligodendrocyte transcription factor 2
- P-TEFb:
-
Positive transcription elongation factor b
- VCF:
-
Variant call format
References
Pratt D, Sahm F, Aldape K (2021) DNA methylation profiling as a model for discovery and precision diagnostics in neuro-oncology. Neuro Oncol 23:S16–S29. https://doi.org/10.1093/neuonc/noab143
Sturm D, Orr BA, Toprak UH, Hovestadt V, Jones DTW, Capper D, Sill M, Buchhalter I, Northcott PA, Leis I et al (2016) New brain tumor entities emerge from molecular classification of CNS-PNETs. Cell 164:1060–1072. https://doi.org/10.1016/j.cell.2016.01.015
Louis DN, Perry A, Wesseling P, Brat DJ, Cree IA, Figarella-Branger D, Hawkins C, Ng HK, Pfister SM, Reifenberger G et al (2021) The 2021 WHO classification of tumors of the central nervous system: a summary. Neuro Oncol 23:1231–1251. https://doi.org/10.1093/neuonc/noab106
Lebrun L, Allard-Demoustiez S, Gilis N, Van Campenhout C, Rodesch M, Roman C, Calo P, Lolli V, David P, Fricx C et al (2023) Clinicopathological and molecular characterization of a case classified by DNA-methylation profiling as “CNS embryonal tumor with BRD4-LEUTX fusion.” Acta Neuropathol Commun 11:46. https://doi.org/10.1186/s40478-023-01549-2
Wong M, Mayoh C, Lau LMS, Khuong-Quang DA, Pinese M, Kumar A, Barahona P, Wilkie EE, Sullivan P, Bowen-James R et al (2020) Whole genome, transcriptome and methylome profiling enhances actionable target discovery in high-risk pediatric cancer. Nat Med 26:1742–1753. https://doi.org/10.1038/s41591-020-1072-4
Capper D, Jones DTW, Sill M, Hovestadt V, Schrimpf D, Sturm D, Koelsche C, Sahm F, Chavez L, Reuss DE et al (2018) DNA methylation-based classification of central nervous system tumours. Nature 555:469–474. https://doi.org/10.1038/nature26000
Northcott PA, Buchhalter I, Morrissy AS, Hovestadt V, Weischenfeldt J, Ehrenberger T, Grobner S, Segura-Wang M, Zichner T, Rudneva VA et al (2017) The whole-genome landscape of medulloblastoma subtypes. Nature 547:311–317. https://doi.org/10.1038/nature22973
Tauziede-Espariat A, Figarella-Branger D, Metais A, Uro-Coste E, Maurage CA, Lhermitte B, Aline-Fardin A, Hasty L, Vasiljevic A, Chiforeanu D et al (2023) CNS neuroblastoma, FOXR2-activated and its mimics: a relevant panel approach for work-up and accurate diagnosis of this rare neoplasm. Acta Neuropathol Commun 11:43. https://doi.org/10.1186/s40478-023-01536-7
Dobin A, Davis CA, Schlesinger F, Drenkow J, Zaleski C, Jha S, Batut P, Chaisson M, Gingeras TR (2013) STAR: ultrafast universal RNA-seq aligner. Bioinformatics 29:15–21. https://doi.org/10.1093/bioinformatics/bts635
Uhrig S, Ellermann J, Walther T, Burkhardt P, Frohlich M, Hutter B, Toprak UH, Neumann O, Stenzinger A, Scholl C et al (2021) Accurate and efficient detection of gene fusions from RNA sequencing data. Genome Res 31:448–460. https://doi.org/10.1101/gr.257246.119
Robinson JT, Thorvaldsdottir H, Wenger AM, Zehir A, Mesirov JP (2017) Variant review with the integrative genomics viewer. Cancer Res 77:e31–e34. https://doi.org/10.1158/0008-5472.CAN-17-0337
Wu Z, Abdullaev Z, Pratt D, Chung HJ, Skarshaug S, Zgonc V, Perry C, Pack S, Saidkhodjaeva L, Nagaraj S et al (2022) Impact of the methylation classifier and ancillary methods on CNS tumor diagnostics. Neuro Oncol 24:571–581. https://doi.org/10.1093/neuonc/noab227
Chakravarthy A, Furness A, Joshi K, Ghorani E, Ford K, Ward MJ, King EV, Lechner M, Marafioti T, Quezada SA et al (2018) Pan-cancer deconvolution of tumour composition using DNA methylation. Nat Commun 9:3220. https://doi.org/10.1038/s41467-018-05570-1
Johann PD, Jager N, Pfister SM, Sill M (2019) RF_Purify: a novel tool for comprehensive analysis of tumor-purity in methylation array data based on random forest regression. BMC Bioinform 20:428. https://doi.org/10.1186/s12859-019-3014-z
Habiba U, Sugino H, Yordanova R, Ise K, Tanei ZI, Ishida Y, Tanikawa S, Terasaka S, Sato KI, Kamoshima Y et al (2021) Loss of H3K27 trimethylation is frequent in IDH1-R132H but not in non-canonical IDH1/2 mutated and 1p/19q codeleted oligodendroglioma: a Japanese cohort study. Acta Neuropathol Commun 9:95. https://doi.org/10.1186/s40478-021-01194-7
Barresi S, Giovannoni I, Rossi S, Stracuzzi A, Quacquarini D, Cafferata B, Piscitelli D, De Leonardis F, Marzullo A, Alaggio R (2021) A novel BRD4-LEUTX fusion in a pediatric sarcoma with epithelioid morphology and diffuse S100 expression. Genes Chromosom Cancer 60:647–652. https://doi.org/10.1002/gcc.22974
Wang R, You J (2015) Mechanistic analysis of the role of bromodomain-containing protein 4 (BRD4) in BRD4-NUT oncoprotein-induced transcriptional activation. J Biol Chem 290:2744–2758. https://doi.org/10.1074/jbc.M114.600759
Thompson-Wicking K, Francis RW, Stirnweiss A, Ferrari E, Welch MD, Baker E, Murch AR, Gout AM, Carter KW, Charles AK et al (2013) Novel BRD4-NUT fusion isoforms increase the pathogenic complexity in NUT midline carcinoma. Oncogene 32:4664–4674. https://doi.org/10.1038/onc.2012.487
Donati B, Lorenzini E, Ciarrocchi A (2018) BRD4 and Cancer: going beyond transcriptional regulation. Mol Cancer 17:164. https://doi.org/10.1186/s12943-018-0915-9
Song K, Huang Y, Xia CD, Zhu HQ, Wang J (2022) A case of CIC-rearranged sarcoma with CIC-LEUTX gene fusion in spinal cord. Neuropathology 42:555–562. https://doi.org/10.1111/neup.12850
Brcic I, Brodowicz T, Cerroni L, Kashofer K, Serbanescu GL, Kasseroler MT, Amann G, Scheipl S, Szkandera J, Leithner A, Liegl-Atzwanger B (2020) Undifferentiated round cell sarcomas with CIC-DUX4 gene fusion: expanding the clinical spectrum. Pathology 52:236–242. https://doi.org/10.1016/j.pathol.2019.09.015
Sievers P, Sill M, Schrimpf D, Abdullaev Z, Donson AM, Lake JA, Friedel D, Scheie D, Tynninen O, Rauramaa T et al (2023) Pediatric-type high-grade neuroepithelial tumors with CIC gene fusion share a common DNA methylation signature. NPJ Precis Oncol 7:30. https://doi.org/10.1038/s41698-023-00372-1
Zhang S, Roeder RG (2020) The long and the short of BRD4: two tales in breast cancer. Mol Cell 78:993–995. https://doi.org/10.1016/j.molcel.2020.05.033
Wu SY, Chiang CM (2007) The double bromodomain-containing chromatin adaptor Brd4 and transcriptional regulation. J Biol Chem 282:13141–13145. https://doi.org/10.1074/jbc.R700001200
Alsarraj J, Walker RC, Webster JD, Geiger TR, Crawford NP, Simpson RM, Ozato K, Hunter KW (2011) Deletion of the proline-rich region of the murine metastasis susceptibility gene Brd4 promotes epithelial-to-mesenchymal transition- and stem cell-like conversion. Cancer Res 71:3121–3131. https://doi.org/10.1158/0008-5472.CAN-10-4417
Duan Y, Guan Y, Qin W, Zhai X, Yu B, Liu H (2018) Targeting Brd4 for cancer therapy: inhibitors and degraders. Medchemcomm 9:1779–1802. https://doi.org/10.1039/c8md00198g
Decock A, Creytens D, Lefever S, Van der Meulen J, Anckaert J, De Ganck A, Deleu J, De Wilde B, Fierro C, Kuersten S et al (2022) mRNA Capture Sequencing and RT-qPCR for the detection of pathognomonic, novel, and secondary fusion transcripts in FFPE tissue: a sarcoma showcase. Int J Mol Sci. https://doi.org/10.3390/ijms231911007
Keck MK, Sill M, Wittmann A, Joshi P, Stichel D, Beck P, Okonechnikow K, Sievers P, Wefers AK, Roncaroli F et al (2023) Amplification of the PLAG-family genes-PLAGL1 and PLAGL2-is a key feature of the novel tumor type CNS embryonal tumor with PLAGL amplification. Acta Neuropathol 145:49–69. https://doi.org/10.1007/s00401-022-02516-2
Wiles ET, Selker EU (2017) H3K27 methylation: a promiscuous repressive chromatin mark. Curr Opin Genet Dev 43:31–37. https://doi.org/10.1016/j.gde.2016.11.001
Acknowledgements
This research is supported in part by the Intramural Research Program of the National Institutes of Health (NIH), National Cancer Institute (NCI), Center for Cancer Research (CCR). This work utilized the computational resources of the NIH HPC Biowulf cluster.
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Open Access funding provided by the National Institutes of Health (NIH).
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KA conceived the study. FA, CKF, SR, ZA, MQ KD, HC, OS, KC and KA participated in data collection, analysis, and manuscript preparation. AP, CG, AT, EG, ASS, NW, CD, DP, PJC, MQ, FA and KA completed pathologic analysis of patients. All authors reviewed and approved manuscript.
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Co-first Authors: Felipe Andreiuolo, Christina K. Ferrone, and Sharika Rajan.
Supplementary Information
Additional file 1: Table S1.
Main histopathologic findings in tumors with BRD4::LEUTX fusion. Table S2. Main immunohistochemical findings in tumors with BRD4::LEUTX fusion.
Additional file 2: Fig. S1.
CNV profiles. A. Case #1; unremarkable. B. Case #2; alterations include gain of 1q21.1q44, loss of 14q23.3q32.33 (including DICER1 and AKT1), and gain of chromosome 19 with a focal loss of 19q13.33q13.43 (including PPP2R1A, CACNG6, C19MC and TTYH1). C. Case #3; unremarkable. D. Case #4; unremarkable.
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Andreiuolo, F., Ferrone, C.K., Rajan, S. et al. Molecular and clinicopathologic characteristics of CNS embryonal tumors with BRD4::LEUTX fusion. acta neuropathol commun 12, 42 (2024). https://doi.org/10.1186/s40478-024-01746-7
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DOI: https://doi.org/10.1186/s40478-024-01746-7