Skip to main content
Download PDF

Assembly of transgenic human P301S Tau is necessary for neurodegeneration in murine spinal cord

Acta Neuropathologica Communications volume 7, Article number: 44 (2019) Cite this article

Abstract

A pathological pathway leading from soluble monomeric to insoluble filamentous Tau is characteristic of many human neurodegenerative diseases, which also exhibit dysfunction and death of brain cells. However, it is unknown how the assembly of Tau into filaments relates to cell loss. To study this, we first used a mouse line transgenic for full-length human mutant P301S Tau to investigate the temporal relationship between Tau assembly into filaments, assessed using anti-Tau antibody AT100, and motor neuron numbers, in the lumbar spinal cord. AT100 immunoreactivity preceded nerve cell loss. Murine Tau did not contribute significantly to either Tau aggregation or neurodegeneration. To further study the relevance of filament formation for neurodegeneration, we deleted hexapeptides 275VQIINK280 and 306VQIVYK311, either singly or in combination, from human 0N4R Tau with the P301S mutation. These hexapeptides are essential for the assembly of Tau into filaments. Homozygous mice transgenic for P301S Tau with the hexapeptide deletions, which expressed Tau at a similar level to the heterozygous line transgenic for P301S Tau, had a normal lifespan, unlike mice from the P301S Tau line. The latter had significant levels of sarkosyl-insoluble Tau in brain and spinal cord, and exhibited neurodegeneration. Mice transgenic for P301S Tau with the hexapeptide deletions failed to show significant levels of sarkosyl-insoluble Tau or neurodegeneration. Recombinant P301S Tau with the hexapeptide deletions failed to form β-sheet structure and filaments following incubation with heparin. Taken together, we conclude that β-sheet assembly of human P301S Tau is necessary for neurodegeneration in transgenic mice.

Introduction

The assembly of Tau protein into abnormal filaments characterises many human neurodegenerative diseases [16]. Assemblies appear to spread through specific neuronal networks in each disease, with short filaments having the greatest seeding activity [22]. Six Tau isoforms are expressed in normal adult human brain – three isoforms with four microtubule-binding repeats each (R1, R2, R3, R4; 4R Tau) and three isoforms lacking R2 (3R Tau) [19]. Each repeat is 31 or 32 amino acids in length. Tau filaments can be composed of either 3R or 4R Tau, or of 3R + 4R Tau. High-resolution structures of human brain Tau filaments made of 3R Tau and of 3R + 4R Tau established the existence of molecular conformers of aggregated Tau [11, 14]. The identification of disease-causing mutations in MAPT, the Tau gene, in cases of frontotemporal dementia, showed that dysfunction of Tau protein is sufficient to cause neurodegeneration and dementia [21, 35, 40].

In humans, mutation P301S in R2 of Tau gives rise to an early-onset form of frontotemporal dementia [5]. We produced and characterised a transgenic mouse line, which expresses full-length human 0N4R P301S Tau, under the control of the murine Thy1 promoter [1]. It exhibits the essential characteristics of human Tauopathies, including Tau hyperphosphorylation, abundant Tau filaments in nerve cells and neurodegeneration. Even though the propagation of Tau pathology correlates best with the presence of short Tau filaments [22], the connection between Tau assembly and neurodegeneration is only incompletely understood. Nerve cell loss in the absence of abundant Tau filaments has been reported in the rTg4510 transgenic mouse line, which expresses high levels of human mutant P301L Tau [37, 41]. A subsequent study showed that memory loss in this line correlated with the presence of soluble Tau oligomers [4].

To examine the temporal relationship between assembly and neurodegeneration, we quantitated Tau assembly and nerve cell loss in the ventral horn of the lumbar spinal cord of mice transgenic for human mutant P301S Tau. We assessed Tau assembly using antibody AT100 [27, 50], immunoreactivity of which is closely associated with Tau filaments rich in β-sheets in adult mouse brain [1, 7]. We counted motor neuron numbers using unbiased stereology. We report that AT100 immunoreactivity preceded nerve cell loss, with murine Tau playing no significant part.

Two hexapeptides (residues 275VQIINK280 in R2 and 306VQIVYK311 in R3) are important for Tau filament formation [25, 38, 39, 45, 46]. The local structure encompassing residues 306–311 regulates Tau assembly, and mutations P301L and P301S increase susceptibility to conformational changes that expose this hexapeptide motif [28]. To further examine the relationship between β-sheet-dependent assembly of Tau and neurodegeneration, we generated homozygous mice transgenic for human P301S Tau that lacked residues 275VQIINK280, 306VQIVYK311 or both hexapeptides and expressed levels of human mutant Tau that were similar to those of the heterozygous line transgenic for full-length P301S Tau. While mice transgenic for full-length P301S Tau had a heavy load of sarkosyl-insoluble Tau in spinal cord at 16 months of age and suffered from a severe paraparesis, the deletion lines did not develop significant levels of sarkosyl-insoluble Tau and had a normal lifespan. In addition, recombinant P301S Tau with these hexapeptide deletions failed to form β-sheet structure and filaments following incubation with heparin.

Materials and methods

Transgenic mice

Homozygous human 0N4R P301S Tau transgenic mice [1] on a pure C57BL/6 JAX background and age-matched C57BL/6 JAX mice were analysed at postnatal day 20, monthly from 1 to 6 months and at 7–8 months (end stage), with 5 animals per group. The minimum number of animals needed was determined by power analysis (GraphPad StatMate) with effect size = 20%, significance level = 0.05, power = 90%. End stage was defined as the presence of a severe paraparesis involving one or both hindlimbs. We also analysed homozygous P301S Tau transgenic mice on a murine Tau null background at end stage (n = 5). This line was created by crossing mice transgenic for human mutant P301S Tau with a murine Tau knockout line [43]. The latter is knockout for most of mouse Tau, but expresses its first 31 amino acids. Following back-crossing with a pure C57BL/6 JAX line for six generations, MAX-BAX analysis (Charles River) showed that the human P301S Tau x mouse Tau knockout line was on a pure C57BL/6 JAX background. Three new transgenic mouse lines expressing 0N4R P301S Tau with either deletions of 275VQIINK280 (line Δ1), 306VQIVYK311 (line Δ2) or both (275VQIINK280 and 306VQIVYK311) (line Δ3) were produced. Site-directed mutagenesis (QuikChange, Agilent Technologies, UK) was used to introduce the hexapeptide deletions into the 0N4R P301S Tau cDNA. Mutated cDNAs were subcloned into pCR2.1 TOPO vectors and a Kozak consensus sequence was introduced upstream of the start codon, followed by subcloning into the XhoI site of the murine Thy1.2 genomic expression vector. Transgenic mice were produced using pronuclear injection of (C57BL/6J x CBA/CA) F1 embryos. Founders were identified by polymerase chain reaction (PCR) of lysates from tail biopsies using 5′- CACAGACACACACCCAGGACATAG-3′ (forward primer) and 5′-CCACCTCCTGGTTTATGATGGATG-3′ (reverse primer). Homozygosity was determined by quantitative PCR and confirmed by mating with wild-type mice.

ELISA

Brains and spinal cords were homogenised in 2 ml/g buffer [25 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 5 mM sodium pyrophosphate, 10 mM β-glycerophosphate, 30 mM sodium fluoride, 10 mM sodium vanadate, 10 mM PMSF and one tablet of complete protease inhibitor cocktail (Roche) per 20 ml buffer], followed by a 30 min centrifugation at 150,000 g. The supernatants from at least three mice per group were diluted 1:150 (30–60 ng protein/ml) in reagent diluent (R&D Systems) and incubated overnight at 4 °C in 96-well ELISA plates, which were blocked with phosphate-buffered saline (PBS) containing 0.2% Tween-20 and 3% bovine serum albumin (BSA) for 1 h at 37 °C. To assess human Tau transgene expression levels, the supernatants were then incubated with human-specific [27] anti-Tau antibody HT7 (1:6,000, Fisher Scientific) for 2 h, followed by horseradish peroxidase-conjugated anti-mouse antibody (1:50,000, Bio-Rad) for 1 h. Following a colorimetric reaction, the plates were read on a Tecan plate reader. Purified recombinant Tau and brain lysates of heterozygous mice transgenic for human mutant P301S Tau were used as standards.

Sarkosyl extraction

The above pellets were resuspended in 10 mM Tris-HCl, pH 7.4, 800 mM NaCl, 1 mM EGTA, 10 mM PMSF, 10% sucrose and one tablet/20 ml buffer of complete protease inhibitor cocktail (Roche). After a 20 min centrifugation at 6,000 g, the supernatants were incubated with 1% sarkosyl (Sigma) for 90 min at room temperature, followed by a 1 h centrifugation at 150,000 g. The sarkosyl-insoluble pellets were resuspended in 150 μl/g tissue 50 mM Tris-HCl, pH 7.4 and stored at 4 °C for immunoblotting.

Immunoblotting

Samples were run on Novex 8% or 10% Tris-Glycine gels (ThermoFisher Scientific) and transferred onto nitrocellulose membranes. The blots were incubated overnight with primary antibodies, followed by either anti-mouse or anti-rabbit HRP conjugated secondary antibodies, and the signal was visualised by enhanced chemiluminescence (GE Healthcare).

Antibodies

To detect assembled Tau, we used antibody AT100 (ThermoFisher Scientific), which recognises Tau phosphorylated at T212, S214 and T217 [50], and is a marker of filamentous Tau in transgenic mouse brain [1, 7]. To quantify motor neuron loss, we used an anti-NeuN antibody (Millipore). AT100 and anti-NeuN were used at 1:500. For immunoblotting, AT100 was used at 1:1,000. T49 (Merck Millipore) [23] was used at 1:50,000 for detection of murine Tau. HT7 (ThermoFisher Scientific) was used at 1:1000 for detection of human Tau [27].

Immunohistochemistry

Mice were perfused transcardially with 4% paraformaldehyde in 0.1 M PBS, pH 7.4. Spinal cords were dissected and post-fixed overnight at 4 °C, followed by cryo-protection in 20% sucrose in PBS for a minimum of 24 h. Serial transverse sections (30 μm) of lumbar spinal cord (L3-L5) were cut on a Leica SM2400 microtome (Leica Microsystems) and stored at 4 °C in PBS containing 0.1% sodium azide. Every twelfth section was processed for immunohistochemistry. Endogenous peroxidase activity was quenched by incubation in 0.3% H2O2 for 30 min. Following a brief wash in PBS + 0.1% Triton X-100 (PBST), the sections were incubated in blocking buffer (PBST + 5% normal horse serum) for 1 h. This was followed by an overnight incubation at 4 °C with primary antibody in blocking buffer. After three rinses with PBST, the sections were incubated with a biotin-conjugated anti-mouse antibody for 1 h at room temperature. Following a further three rinses with PBST, the avidin-biotin-conjugated complex was applied for 1 h. The antigen was visualised with the Vector VIP substrate kit (Vector Laboratories). Tissue sections were mounted on frosted end glass slides (ThermoScientific) and coverslipped.

Tau expression, purification and assembly

Following subcloning into pRK172, expression and purification of recombinant 0N4R P301S Tau, 0N4R P301S Tau lacking residues 275VQIINK280 (Δ1), 306VQIVYK311 (Δ2), or both hexapeptides (Δ3) were done as described [10, 18]. Tau concentrations were determined by amino acid analysis. For filament assembly, Tau proteins (135 μg/ml) were incubated with heparin (average molecular weight: 17–19 kDa, Sigma) in PBS with 1 mM EDTA, 1 mM AEBSF and 1 mM TCEP at 37 °C for 72 h, as described [31]. The molar ratio of Tau:heparin was approximately 4:1. The kinetics of Tau assembly were monitored by the fluorescence of Thioflavin T (ThT), as described [10, 15, 49].

Electron microscopy

For a semi-quantitative assessment of Tau filaments, negative-stain electron microscopy was used, as described [12]. Briefly, aliquots of heparin-induced assembled recombinant Tau were negatively stained with 2% uranyl acetate and observed using a Tecnai G2 Spirit TEM. Images were taken at × 6500 magnification.

Stereology

Following a pilot study using the optical fractionator probe of StereoInvestigator 11 (MBF Bioscience), we opted for a total of eight sections/animal spanning L3-L5 of the spinal cord, in a random manner from a total of 96 sections (using 1:12). Section thickness was determined using StereoInvestigator 11 software. For each section, the outline of the region of interest was traced under a × 5 objective starting from the middle of the central canal and contouring the grey matter of the ventral horn. The average area of the delineated region was 351,498 ± 3983 μm2 for the P301S Tau group and 361,536 ± 7497 μm2 for the wild-type group (the difference between the two groups was not significant). For the human P301S Tau x mouse Tau knockout line, the average area sampled was 308,041 ± 13,436 μm2; it was 326,447 ± 17,847 μm2 for the age-matched human P301S Tau mice (the difference between the two groups was not significant). By using the optical fractionator probe (grid size 65 × 65 μm2; height 22 μm; guard height 3 μm; counting frame 50 × 50 μm2), NeuN-positive cells with a diameter of at least 30 μm and their nuclei within the dissector volume were counted using the × 100 objective, and the number of motor neurons per lumbar spinal cord calculated. The investigator was blinded with respect to the nature of the groups. Statistical analyses were performed using a one-way ANOVA test, followed by Tukey’s multiple comparisons test (Prism, GraphPad Software, Inc.).

Image quantitation

Photographs were taken using an Olympus BX41 microscope equipped with a Nikon Digital Sight DS-2Mv digital camera under a × 20 objective. Each picture amounted to 1600 × 1200 pixels. AT100 immunoreactivity was quantitated using the green channel of ImageJ (NIH). The threshold was set to: 134 in greyscale, and the circularity to: 0-infinity.

Results

AT100 immunoreactivity and motor neuron loss in mice transgenic for human P301S tau

Sections from the lumbar spinal cord of full-length human 0N4R P301S Tau transgenic mice were stained with antibody AT100 to identify Tau aggregates and anti-NeuN to detect motor neurons. The same was done for age-matched wild-type mice.

Figs. 1 and 2 show the time course of AT100 immunostaining qualitatively and quantitatively, respectively. There was no staining in wild-type and 20-day-old (P20) transgenic mice, indicating the absence of Tau aggregates. However, in 1-month-old transgenic mice, a small number of AT100-positive, dot-like structures, was present in neurites throughout the lumbar spinal cord. A similar picture was observed at 2 months, but the number of dot-like structures had increased (p < 0.05 relative to P20, using 1-way ANOVA). Prominent cell body staining for AT100 was observed in addition to increased neuritic staining in transgenic mice aged 3 months (p < 0.001 relative to P20). The number of immunoreactive nerve cell bodies and terminals steadily increased between 3 months and the end stage at 7–8 months of age.

Fig. 1
figure 1

AT100 immunoreactivity in ventral horn of the lumbar spinal cord of mice transgenic for full-length human P301S Tau as a function of time. Insets depict the framed areas at higher magnification. Note the absence of AT100 immunoreactivity in (a) and the presence of stained dot-like structures in (b) and (c) (arrowed), prior to cell body staining (d-h). Scale bars, 100 μm

Full size image
Fig. 2
figure 2

Quantitation of AT100 immunoreactivity in ventral horn of the lumbar spinal cord of mice transgenic for full-length human P301S Tau as a function of time (5 animals/group). The values at 7–8 months (end stage) are taken as 100%. One-way ANOVA [F (4,20) = 66.17, p < 0.0001], followed by Tukey’s multiple comparison test between P20 mice and those aged 1, 2, 3 or 4 months; n.s. = not significant; *p < 0.05; ***p < 0.001; ****p < 0.0001. The results are expressed as means ± S.E.M

Full size image

Figs. 3 and 4 show the time course of NeuN staining. The number of motor neurons in transgenic P301S Tau mice aged 20 days, 1 month and 2 months did not differ significantly from one another or from the number of motor neurons in wild-type mice. In transgenic mice, significant motor neuron loss (22% reduction relative to P20) was first observed at 3 months of age. It reached 41% at 4 months, 51% at 5 months, 60% at 6 months and 69% at 7–8 months. A Pearson product-moment correlation coefficient was computed to assess the nature of the relationship between AT100 immunoreactivity and number of motor neurons. A significant negative correlation was seen, r = − 0.85, n = 5, p = 0.007.

Fig. 3
figure 3

NeuN immunoreactivity in ventral horn of the lumbar spinal cord of mice transgenic for full-length human P301S Tau as a function of time. Motor neurons are large cells in the ventral horn (circled with a dashed line in b). Scale bars, 100 μm

Full size image
Fig. 4
figure 4

Quantitation of motor neuron numbers in ventral horn of the lumbar spinal cord of mice transgenic for full-length human P301S Tau as a function of time (5 animals/group). The values at P20 are taken as 100%. One-way ANOVA [F (7,32) = 152.1, p < 0.0001], followed by Dunnett’s multiple comparison test; n.s. = not significant; ****p < 0.0001. The results are expressed as means ± S.E.M

Full size image

The relevance of murine tau in mice transgenic for human P301S tau

The contribution of murine Tau to aggregation and loss of motor neurons was investigated by comparing the lumbar spinal cords of end stage human P301S Tau mice and human P301S Tau x mouse Tau knockout mice (P301S Tau x mTau KO). Quantitation of AT100 immunoreactivity showed no significant differences between the two groups (Fig. 5a). The same was true when motor neuron numbers were counted using unbiased stereology (Fig. 5b). Additionally, the average survival times of human P301S Tau mice were similar to those of human P301S Tau x mTau KO mice (n = 20) (Fig. 5c). Lastly, upon extracting Tau filaments from end stage P301S Tau and P301S Tau x mTau KO spinal cords, and running these extracts by immunoblotting, both lines showed similar levels of filamentous Tau (as judged by AT100 immunoreactivity). Aggregates were made of human mutant Tau, since no murine Tau was detected in the aggregates, as judged by the lack of T49 immunoreactivity (Fig. 5d).

Fig. 5
figure 5

Relevance of murine Tau for assembly and neurodegeneration. The values of AT100 immunoreactivity (a) and motor neuron numbers (b) of homozygous 7–8-month-old mice transgenic for full-length human P301S Tau on a normal murine Tau background are taken as 100%. The results are expressed as means ± S.E.M. (5 animals/group). Two-tail unpaired student t-test revealed no significant difference (p = 0.78 and p = 0.85 respectively). c Survival of mice transgenic for full-length human P301S Tau compared to those on a murine Tau null background (20 animals/group). Two-tail unpaired student t-test revealed no significant difference (p = 0.09). n.s., not significant. d Immunoblots of sarkosyl-insoluble Tau from spinal cord of 7–8-month-old mice transgenic for full-length human P301S Tau, in presence (lane 1) or absence (lane 2) of murine Tau. Lane 3 shows lack of immunoreactivity in an age-matched wild-type mouse. Antibody AT100 is specific for Tau phosphorylated at T212/S214/T217 and identifies filamentous Tau. T49 recognises murine Tau, whereas HT7 is specific for human Tau

Full size image

Filament assembly of human P301S tau is required for neurodegeneration in transgenic mice

To examine the relationship between β-sheet-dependent filament assembly of Tau and neurodegeneration, we generated mice transgenic for P301S Tau with deletion of residues 275VQIINK280 (line Δ1), 306VQIVYK311 (line Δ2), or 275VQIINK280 and 306VQIVYK311 (line Δ3), as shown in Fig. 6. After assessing the expression levels of human mutant Tau in lines Δ1-Δ3 relative to those in the heterozygous full-length P301S Tau transgenic line by ELISA (Fig. 7a), the presence of sarkosyl-insoluble Tau in brain was investigated. While abundant sarkosyl-insoluble Tau was present in the full-length P301S Tau transgenic line at 16 months, none was seen in the Δ2 and Δ3 lines at 24 months of age. A small amount of sarkosyl-insoluble Tau was present in mice from line Δ1 (Fig. 7b, c) at 24 months, which showed an uncoordinated gait at this age, characteristic of the early stages of phenotype. Heterozygous P301S Tau mice usually reach end stage 3 to 4 months following onset of phenotype. Mice from the full-length P301S Tau line suffered a severe paraparesis and reached end stage at around 16–19 months of age, whereas mice from lines Δ2 and Δ3 were fully mobile at 24 months and had a normal lifespan (Fig. 7d).

Fig. 6
figure 6

Schematic of constructs used to generate transgenic lines and recombinant Tau. Four variants were made: 1) Full-length human 0N4R Tau with the P301S mutation (P301S Tau), 2) 0N4R P301S Tau with deletion of 275VQIINK280 (Δ1), 3) 0N4R P301S Tau with deletion of 306VQIVYK311 (Δ2), and 4) 0N4R P301S Tau with deletions of 275VQIINK280 and 306VQIVYK311 (Δ3)

Full size image
Fig. 7
figure 7

Mice transgenic for full-length human P301S Tau and for Δ1, Δ2, Δ3. a Relative levels of transgene expression, as measured by ELISA, with those of full-length human P301S Tau taken as 100%. One-way ANOVA [F (3,16) = 6.109, p = 0.0057], followed by Dunnett’s multiple comparison test (b) Sarkosyl-insoluble Tau in the brains of mice transgenic for human P301S Tau (16 months) and transgenic for Δ1, Δ2 and Δ3 (24 months) immunoblotted for AT100. The n numbers were as follows: 3 for P301S Tau, 5 for Δ1, 5 for Δ2, and 5 for Δ3. The arrow points to low levels of sarkosyl-insoluble Tau in line Δ1. c Quantitation of the sarkosyl-insoluble samples run by Western Blot, with the values for P301S Tau taken as 100%. One-way ANOVA [F (3,14) = 18.19, p < 0.0001], followed by Tukey’s multiple comparisons test. The results are expressed as means ± SEM. *** p < 0.001, n.s. = not significant. d Survival plot of the human P301S Tau transgenic line compared to lines Δ1, Δ2, and Δ3

Full size image

Human P301S tau assembly in vitro requires β-sheet structure

Unlike recombinant 0N4R P301S Tau, 0N4R P301S Tau lacking residues 275VQIINK280 (Δ1), 306VQIVYK311 (Δ2) or both hexapeptides (Δ3) failed to form β-sheet structure (Fig. 8a) or filaments (Fig. 8c) following incubation with heparin.

Fig. 8
figure 8

Heparin-induced assembly of recombinant Tau. a Heparin-induced assembly of recombinant Tau, monitored over time using Thioflavin T fluorescence. P301S Tau is shown in orange, Δ1 in black, Δ2 in red and Δ3 in green. b Coomassie-stained gel of purified proteins. c Negative stain electron microscopy of assembled recombinant Tau. Note that full-length recombinant P301S Tau assembled into filaments, but that Δ1, Δ2, and Δ3 proteins did not form filaments. Scale bars, 200 nm

Full size image

Discussion

Neurodegeneration and propagation of pathology are hallmarks of human tauopathies [16]. Mice transgenic for human mutant P301S Tau exhibit major features of the human diseases, including abundant filamentous Tau inclusions and nerve cell loss [1]. Because of its consistency, this mouse line has been found to be suitable for drug discovery studies [47]. However, the relationship between Tau aggregation and nerve cell loss is unknown. Here we show an inverse correlation between AT100 immunoreactivity and the number of motor neurons in the lumbar spinal cord of mice transgenic for human P301S Tau.

We showed previously that AT100 detects filamentous human Tau in transgenic mice [1, 6, 7]. It remains to be seen if it also detects other forms of Tau aggregates, such as oligomers. Although AT100 is a phosphorylation-dependent anti-Tau antibody (pT212/pS214/pT217), it only labelled insoluble non-sonicated Tau in mice transgenic for human P301S Tau [1, 7]. By immunohistochemistry, AT100 detected predominantly dot-like, neuritic inclusions in human P301S Tau transgenic mice aged 1–2 months. These findings suggest that Tau assemblies form in nerve terminals innervating muscle, before they appear in nerve cell bodies, as reported in another line of mice transgenic for human P301S Tau [48]. From 3 months onwards, the number of Tau aggregates increased in both terminals and cell bodies. End stage P301S Tau mice have neurogenic muscle atrophy [1]. P301S Tau transgenic mice and AT100 immunoreactivity have been instrumental for the experimental demonstration of the prion-like properties of aggregated Tau [6] and the finding that short Tau filaments have the greatest seeding activity [22]. Previous experiments in a mouse model of Tauopathy reported seeding activity before detectable neuropathology or accumulation of insoluble protein [20]. A study using a different mouse line transgenic for human P301S Tau reported the presence of Tau seeds in the brain at 3–5 weeks of age [47]. This agrees with the presence of AT100-immunoreactive structures in the spinal cord at 4 weeks reported here. It remains to be seen when Tau seeds can first be detected in the lumbar spinal cord of our mice transgenic for human P301S Tau.

Unlike AT100-immunoreactive structures, which were present from 1 month onwards, significant motor neuron loss was only present at 3 months of age. Thus, intraneuronal build-up of Tau aggregates may have been necessary for nerve cell loss. Recent studies report that Tau can disrupt nucleocytoplasmic transport [9, 33]. However, we did not observe any obvious disruption of nucleocytoplasmic transport or change in nuclear morphology in mice transgenic for human mutant P301S Tau. Motor neuron loss increased in parallel with the accumulation of AT100 immunoreactivity, with a 69% reduction at end stage. A correlation between AT100 immunoreactivity and motor neuron loss was observed, suggesting a causal connection. Secondary nucleation processes may also have played a role [24, 42].

The relevance of murine Tau for end stage AT100 immunoreactivity, motor neuron loss, and survival was investigated using human P301S Tau transgenic mice that had been crossed with a mouse Tau knockout line. The presence of murine Tau played no significant part in the above, consistent with Tau inclusions being made almost exclusively of mutant human Tau [1] and the P301S mutation increasing the aggregation propensity of Tau [17, 26]. In humans with frontotemporal dementia caused by the P301L mutation in MAPT, only protein produced from the mutant allele was found in Tau inclusions [29]. Moreover, aggregates of P301L Tau could seed the aggregation of P301L Tau, but not of wild-type Tau [2].

To further analyse the relationship between Tau aggregation and neurodegeneration, mice transgenic for human P301S Tau that lacked residues 275–280, residues 306–311 and both hexapeptides were generated and analysed. These sequences are necessary for heparin-induced filament formation of wild-type Tau in vitro [25, 45, 46]. This is, to our knowledge, the first report detailing the in vivo effects of such hexapeptide deletions in a transgenic model expressing full-length human mutant Tau. Having quantitated the levels of expression of human P301S Tau in the different transgenic lines, we observed a small amount of sarkosyl-insoluble tau in the brains of mice transgenic for Δ1 at 24 months of age. This was in agreement with an in vitro study showing that residues 306VQIVYK311, but not 275VQIINK280, were necessary for heparin-induced assembly of wild-type K18, the 4 repeat domain of human Tau (residues 244–372), into filaments [25]. One might therefore expect a longer incubation time of Δ1 with heparin to eventually yield a small number of Tau filaments. This would not be the case for Δ2 and Δ3. These findings are reminiscent of Drosophila lines expressing human wild-type Tau (0N4R) lacking residues 306–311 that developed no detectable neurodegeneration and significantly less hyperphosphorylated Tau than fly lines expressing full-length Tau [34]. We failed to observe significant levels of sarkosyl-insoluble Tau in mouse lines Δ2 and Δ3 at 24 months of age. As described before, mice transgenic for full-length P301S Tau developed abundant Tau filaments, nerve cell loss and a severe paraparesis at 16–19 months of age. None of the Δ1-Δ3 lines developed motor impairment.

High-resolution structures of the cores of Tau filaments assembled from wild-type recombinant 4R Tau and heparin have been shown to be polymorphic [51]. The most common structure extends from residues 272–330 of Tau and encompasses residues 275–280 and 306–311. P301 is located in the partially disordered hammerhead arc. Since proline residues interrupt hydrogen bond interactions across filament rungs, replacing P301 with L or S may facilitate filament formation by stabilising local structure. Recombinant Tau mutated at residue 301 (P to L or S) forms significantly more heparin-induced filaments than wild-type protein [17].

Unlike human P301S Tau, the expression of one isoform of wild-type human Tau in transgenic mice does not lead to filament formation or neurodegeneration. We show here that deletion of residues 275VQIINK280 and 306VQIVYK311 prevents the assembly of human P301S Tau in transgenic mice. Similar findings have been reported in a cell model of seeded Tau aggregation [10]. Interestingly, deletion of amino acid 280 (ΔK280) results in a significantly greater propensity of Tau to assemble into filaments [3, 36]. This deletion causes frontotemporal dementia in humans, but probably through a mechanism involving mRNA splicing [44]. It thus appears that the ΔK280 mutation increases filament assembly of recombinant Tau, whereas its deletion in the absence of residues 275VQIIN279 abolishes filament assembly. However in vivo, expression of full-length ΔK280 Tau did not yield Tau filaments or overt neurodegeneration [8]. Our findings are reminiscent of those of Mocanu [30], in which mice transgenic for the K18 Tau fragment with ΔK280 showed Tau filaments and nerve cell loss. Since most in vitro studies of Tau assembly were carried out in the presence of heparin, and since monomeric Tau is very soluble, other cofactors and/or post-translational modifications may be required for the assembly of human P301S Tau in brain [12, 13, 32]. It will be interesting to determine high-resolution structures of wild-type and mutant 4R Tau filaments.

Taken together, the present findings establish a close correlation between Tau assembly and neurodegeneration in mice transgenic for human mutant P301S Tau.

References

  1. Allen B, Ingram E, Takao M, Smith MJ, Jakes R, Virdee K, Yoshida H, Holzer M, Craxton M, Emson PC, Atzori C, Migheli A, Crowther RA, Ghetti B, Spillantini MG, Goedert M (2002) Abundant tau filaments and nonapoptotic neurodegeneration in transgenic mice expressing human P301S tau protein. J Neurosci 22:9340–9351

    Article  CAS  Google Scholar 

  2. Aoyagi H, Hasegawa M, Tamaoka A (2007) Fibrillogenic nuclei composed of P301L mutant tau induce elongation of P301L tau but not wild-type tau. J Biol Chem 282:20309–20318. https://doi.org/10.1074/jbc.M611876200

  3. Barghorn S, Davies P, Mandelkow E (2004) Tau paired helical filaments from Alzheimer's disease brain and assembled in vitro are based on beta-structure in the core domain. Biochemistry 43:1694–1703. https://doi.org/10.1021/bi0357006

    Article  CAS  PubMed  Google Scholar 

  4. Berger Z, Roder H, Hanna A, Carlson A, Rangachari V, Yue M, Wszolek Z, Ashe K, Knight J, Dickson D, Andorfer C, Rosenberry TL, Lewis J, Hutton M, Janus C (2007) Accumulation of pathological tau species and memory loss in a conditional model of tauopathy. J Neurosci 27:3650–3662. https://doi.org/10.1523/jneurosci.0587-07.2007

    Article  CAS  PubMed  Google Scholar 

  5. Bugiani O, Murrell JR, Giaccone G, Hasegawa M, Ghigo G, Tabaton M, Morbin M, Primavera A, Carella F, Solaro C, Grisoli M, Savoiardo M, Spillantini MG, Tagliavini F, Goedert M, Ghetti B (1999) Frontotemporal dementia and corticobasal degeneration in a family with a P301S mutation in tau. J Neuropathol Exp Neurol 58:667–677

    Article  CAS  Google Scholar 

  6. Clavaguera F, Bolmont T, Crowther RA, Abramowski D, Frank S, Probst A, Fraser G, Stalder AK, Beibel M, Staufenbiel M, Jucker M, Goedert M, Tolnay M (2009) Transmission and spreading of tauopathy in transgenic mouse brain. Nature Cell Biol 11:909–913. https://doi.org/10.1038/ncb1901

    Article  CAS  PubMed  Google Scholar 

  7. Delobel P, Lavenir I, Fraser G, Ingram E, Holzer M, Ghetti B, Spillantini MG, Crowther RA, Goedert M (2008) Analysis of tau phosphorylation and truncation in a mouse model of human tauopathy. Am J Pathol 172:123–131. https://doi.org/10.2353/ajpath.2008.070627

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Eckermann K, Mocanu MM, Khlistunova I, Biernat J, Nissen A, Hofmann A, Schönig K, Bujard H, Haemisch A, Mandelkow E, Zhou L, Rune G, Mandelkow EM (2007) The beta-propensity of tau determines aggregation and synaptic loss in inducible mouse models of tauopathy. J Biol Chem 282:31755–31765. https://doi.org/10.1074/jbc.M705282200

  9. Eftekharzadeh B, Daigle JG, Kapinos LE, Coyne A, Schiantarelli J, Carlomagno Y, Cook C, Miller SJ, Dujardin S, Amaral AS, Grima JC, Bennett RE, Tepper K, DeTure M, Vanderburg CR, Corjuc BT, DeVos SL, Gonzalez JA, Chew J, Vidensky S, Gage FH, Mertens J, Troncoso J, Mandelkow E, Salvatella X, Lim RYH, Petrucelli L, Wegmann S, Rothstein JD, Hyman BT (2018) Tau protein disrupts nucleocytoplasmic transport in Alzheimer's disease. Neuron 99 e927:925–940. https://doi.org/10.1016/j.neuron.2018.07.039

    Article  CAS  Google Scholar 

  10. Falcon B, Cavallini A, Angers R, Glover S, Murray TK, Barnham L, Jackson S, O'Neill MJ, Isaacs AM, Hutton ML, Szekeres PG, Goedert M, Bose S (2015) Conformation determines the seeding potencies of native and recombinant tau aggregates. J Biol Chem 290:1049–1065. https://doi.org/10.1074/jbc.M114.589309

  11. Falcon B, Zhang W, Murzin AG, Murshudov G, Garringer HJ, Vidal R, Crowther RA, Ghetti B, Scheres SHW, Goedert M (2018) Structures of filaments from Pick's disease reveal a novel tau protein fold. Nature 561:137–140. https://doi.org/10.1038/s41586-018-0454-y

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Falcon B, Zhang W, Schweighauser M, Murzin AG, Vidal R, Garringer HJ, Ghetti B, Scheres SHW, Goedert M (2018) Tau filaments from multiple cases of sporadic and inherited Alzheimer's disease adopt a common fold. Acta Neuropathol 136:699–708. https://doi.org/10.1007/s00401-018-1914-z

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Fichou Y, Lin Y, Rauch JN, Vigers M, Zeng Z, Srivastava M, Keller TJ, Freed JH, Kosik KS, Han S (2018) Cofactors are essential constituents of stable and seeding-active tau fibrils. Proc Natl Acad Sci U S A 115:13234–13239. https://doi.org/10.1073/pnas.1810058115

    Article  CAS  PubMed  Google Scholar 

  14. Fitzpatrick AWP, Falcon B, He S, Murzin AG, Murshudov G, Garringer HJ, Crowther RA, Ghetti B, Goedert M, Scheres SHW (2017) Cryo-EM structures of tau filaments from Alzheimer's disease. Nature 547:185–190. https://doi.org/10.1038/nature23002

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Friedhoff P, Schneider A, Mandelkow EM, Mandelkow E (1998) Rapid assembly of Alzheimer-like paired helical filaments from microtubule-associated protein tau monitored by fluorescence in solution. Biochemistry 37:10223–10230. https://doi.org/10.1021/bi980537d

    Article  CAS  PubMed  Google Scholar 

  16. Goedert M, Eisenberg DS, Crowther RA (2017) Propagation of tau aggregates and neurodegeneration. Annu Rev Neurosci 40:189–210. https://doi.org/10.1146/annurev-neuro-072116-031153

    Article  CAS  PubMed  Google Scholar 

  17. Goedert M, Jakes R, Crowther RA (1999) Effects of frontotemporal dementia FTDP-17 mutations on heparin-induced assembly of tau filaments. FEBS Lett 450:306–311

    Article  CAS  Google Scholar 

  18. Goedert M, Jakes R, Spillantini MG, Hasegawa M, Smith MJ, Crowther RA (1996) Assembly of microtubule-associated protein tau into Alzheimer-like filaments induced by sulphated glycosaminoglycans. Nature 383:550–553. https://doi.org/10.1038/383550a0

    Article  CAS  PubMed  Google Scholar 

  19. Goedert M, Spillantini MG, Jakes R, Rutherford D, Crowther RA (1989) Multiple isoforms of human microtubule-associated protein tau: sequences and localization in neurofibrillary tangles of Alzheimer's disease. Neuron 3:519–526

    Article  CAS  Google Scholar 

  20. Holmes BB, Furman JL, Mahan TE, Yamasaki TR, Mirbaha H, Eades WC, Belaygorod L, Cairns NJ, Holtzman DM, Diamond MI (2014) Proteopathic tau seeding predicts tauopathy in vivo. Proc Natl Acad Sci U S A 111:E4376–E4385. https://doi.org/10.1073/pnas.1411649111

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Hutton M, Lendon CL, Rizzu P, Baker M, Froelich S, Houlden H, Pickering-Brown S, Chakraverty S, Isaacs A, Grover A, Hackett J, Adamson J, Lincoln S, Dickson D, Davies P, Petersen RC, Stevens M, de Graaff E, Wauters E, van Baren J, Hillebrand M, Joosse M, Kwon JM, Nowotny P, Che LK, Norton J, Morris JC, Reed LA, Trojanowski J, Basun H, Lannfelt L, Neystat M, Fahn S, Dark F, Tannenberg T, Dodd PR, Hayward N, Kwok JB, Schofield PR, Andreadis A, Snowden J, Craufurd D, Neary D, Owen F, Oostra BA, Hardy J, Goate A, van Swieten J, Mann D, Lynch T, Heutink P (1998) Association of missense and 5’-splice-site mutations in tau with the inherited dementia FTDP-17. Nature 393:702–705. https://doi.org/10.1038/31508

    Article  CAS  Google Scholar 

  22. Jackson SJ, Kerridge C, Cooper J, Cavallini A, Falcon B, Cella CV, Landi A, Szekeres PG, Murray TK, Ahmed Z, Goedert M, Hutton M, O'Neill MJ, Bose S (2016) Short fibrils constitute the major species of seed-competent tau in the brains of mice transgenic for human P301S tau. J Neurosci 36:762–772. https://doi.org/10.1523/jneurosci.3542-15.2016

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Kosik KS, Orecchio LD, Binder L, Trojanowski JQ, Lee VM, Lee G (1988) Epitopes that span the tau molecule are shared with paired helical filaments. Neuron 1:817–825

    Article  CAS  Google Scholar 

  24. Kundel F, De S, Flagmeier P, Horrocks MH, Kjaergaard M, Shammas SL, Jackson SE, Dobson CM, Klenerman D (2018) Hsp70 inhibits the nucleation and elongation of tau and sequesters tau aggregates with high affinity. ACS Chem Biol 13:636–646. https://doi.org/10.1021/acschembio.7b01039

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Li W, Lee VM (2006) Characterization of two VQIXXK motifs for tau fibrillization in vitro. Biochemistry 45:15692–15701. https://doi.org/10.1021/bi061422+

    Article  CAS  PubMed  Google Scholar 

  26. Maeda S, Sato Y, Takashima A (2018) Frontotemporal dementia with parkinsonism linked to chromosome-17 mutations enhance tau oligomer formation. Neurobiol Aging 69:26–32. https://doi.org/10.1016/j.neurobiolaging.2018.04.014

    Article  CAS  PubMed  Google Scholar 

  27. Mercken M, Vandermeeren M, Lübke U, Six J, Boons J, Van de Voorde A, Martin JJ, Gheuens J (1992) Monoclonal antibodies with selective specificity for Alzheimer tau are directed against phosphatase-sensitive epitopes. Acta Neuropathol 84:265–272

  28. Mirbaha H, Chen D, Morazova OA, Ruff KM, Sharma AM, Liu X, Goodarzi M, Pappu RV, Colby DW, Mirzaei H, Joachimiak LA, Diamond MI (2018) Inert and seed-competent tau monomers suggest structural origins of aggregation. eLife 7:e36584. https://doi.org/10.7554/eLife.36584

    Article  PubMed  PubMed Central  Google Scholar 

  29. Miyasaka T, Morishima-Kawashima M, Ravid R, Kamphorst W, Nagashima K, Ihara Y (2001) Selective deposition of mutant tau in the FTDP-17 brain affected by the P301L mutation. J Neuropathol Exp Neurol 60:872–884

    Article  CAS  Google Scholar 

  30. Mocanu MM, Nissen A, Eckermann K, Khlistunova I, Biernat J, Drexler D, Petrova O, Schönig K, Bujard H, Mandelkow E, Zhou L, Rune G, Mandelkow EM (2008) The potential for beta-structure in the repeat domain of tau protein determines aggregation, synaptic decay, neuronal loss, and coassembly with endogenous tau in inducible mouse models of tauopathy. J Neurosci 28:737–748. https://doi.org/10.1523/jneurosci.2824-07.2008

  31. Morozova OA, March ZM, Robinson AS, Colby DW (2013) Conformational features of tau fibrils from Alzheimer's disease brain are faithfully propagated by unmodified recombinant protein. Biochemistry 52:6960–6967. https://doi.org/10.1021/bi400866w

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. Mudher A, Colin M, Dujardin S, Medina M, Dewachter I, Alavi Naini SM, Mandelkow EM, Mandelkow E, Buée L, Goedert M, Brion JP (2017) What is the evidence that tau pathology spreads through prion-like propagation? Acta Neuropathol Commun 5:99. https://doi.org/10.1186/s40478-017-0488-7

  33. Paonessa F, Evans LD, Solanki R, Larrieu D, Wray S, Hardy J, Jackson SP, Livesey FJ (2019) Microtubules deform the nuclear membrane and disrupt nucleocytoplasmic transport in tau-mediated frontotemporal dementia. Cell Rep 26:582–593.e585. https://doi.org/10.1016/j.celrep.2018.12.085

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  34. Passarella D, Goedert M (2018) Beta-sheet assembly of tau and neurodegeneration in Drosophila melanogaster. Neurobiol Aging 72:98–105. https://doi.org/10.1016/j.neurobiolaging.2018.07.022

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  35. Poorkaj P, Bird TD, Wijsman E, Nemens E, Garruto RM, Anderson L, Andreadis A, Wiederholt WC, Raskind M, Schellenberg GD (1998) Tau is a candidate gene for chromosome 17 frontotemporal dementia. Ann Neurol 43:815–825. https://doi.org/10.1002/ana.410430617

    Article  CAS  PubMed  Google Scholar 

  36. Rizzu P, Van Swieten JC, Joosse M, Hasegawa M, Stevens M, Tibben A, Niermeijer MF, Hillebrand M, Ravid R, Oostra BA, Goedert M, van Duijn CM, Heutink P (1999) High prevalence of mutations in the microtubule-associated protein tau in a population study of frontotemporal dementia in the Netherlands. Am J Hum Genet 64:414–421. https://doi.org/10.1086/302256

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  37. Santacruz K, Lewis J, Spires T, Paulson J, Kotilinek L, Ingelsson M, Guimaraes A, DeTure M, Ramsden M, McGowan E, Forster C, Yue M, Orne J, Janus C, Mariash A, Kuskowski M, Hyman B, Hutton M, Ashe KH (2005) Tau suppression in a neurodegenerative mouse model improves memory function. Science 309:476–481. https://doi.org/10.1126/science.1113694

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. Sawaya MR, Sambashivan S, Nelson R, Ivanova MI, Sievers SA, Apostol MI, Thompson MJ, Balbirnie M, Wiltzius JJ, McFarlane HT, Madsen AO, Riekel C, Eisenberg D (2007) Atomic structures of amyloid cross-beta spines reveal varied steric zippers. Nature 447:453–457. https://doi.org/10.1038/nature05695

    Article  CAS  PubMed  Google Scholar 

  39. Seidler PM, Boyer DR, Rodriguez JA, Sawaya MR, Cascio D, Murray K, Gonen T, Eisenberg DS (2018) Structure-based inhibitors of tau aggregation. Nat Chem 10:170–176. https://doi.org/10.1038/nchem.2889

    Article  CAS  PubMed  Google Scholar 

  40. Spillantini MG, Murrell JR, Goedert M, Farlow MR, Klug A, Ghetti B (1998) Mutation in the tau gene in familial multiple system tauopathy with presenile dementia. Proc Natl Acad Sci U S A 95:7737–7741

    Article  CAS  Google Scholar 

  41. Spires TL, Orne JD, SantaCruz K, Pitstick R, Carlson GA, Ashe KH, Hyman BT (2006) Region-specific dissociation of neuronal loss and neurofibrillary pathology in a mouse model of tauopathy. Am J Pathol 168:1598–1607. https://doi.org/10.2353/ajpath.2006.050840

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  42. Tornquist M, Michaels TCT, Sanagavarapu K, Yang X, Meisl G, Cohen SIA, Knowles TPJ, Linse S (2018) Secondary nucleation in amyloid formation. Chem Commun 54:8667–8684. https://doi.org/10.1039/c8cc02204f

    Article  CAS  Google Scholar 

  43. Tucker KL, Meyer M, Barde YA (2001) Neurotrophins are required for nerve growth during development. Nat Neurosci 4:29–37. https://doi.org/10.1038/82868

    Article  CAS  PubMed  Google Scholar 

  44. van Swieten JC, Bronner IF, Azmani A, Severijnen LA, Kamphorst W, Ravid R, Rizzu P, Willemsen R, Heutink P (2007) The DeltaK280 mutation in MAP tau favors exon 10 skipping in vivo. J Neuropathol Exp Neurol 66:17–25. https://doi.org/10.1097/nen.0b013e31802c39a4

    Article  PubMed  Google Scholar 

  45. von Bergen M, Barghorn S, Li L, Marx A, Biernat J, Mandelkow EM, Mandelkow E (2001) Mutations of tau protein in frontotemporal dementia promote aggregation of paired helical filaments by enhancing local beta-structure. J Biol Chem 276:48165–48174. https://doi.org/10.1074/jbc.M105196200

  46. von Bergen M, Friedhoff P, Biernat J, Heberle J, Mandelkow EM, Mandelkow E (2000) Assembly of tau protein into Alzheimer paired helical filaments depends on a local sequence motif ((306) VQIVYK (311)) forming beta structure. Proc Natl Acad Sci U S A 97:5129–5134

    Article  Google Scholar 

  47. Woerman AL, Patel S, Kazmi SA, Oehler A, Freyman Y, Espiritu L, Cotter R, Castaneda JA, Olson SH, Prusiner SB (2017) Kinetics of human mutant tau prion formation in the brains of 2 transgenic mouse lines. JAMA Neurol 74:1464–1472. https://doi.org/10.1001/jamaneurol.2017.2822

    Article  PubMed  PubMed Central  Google Scholar 

  48. Yin ZR, Valkenburg F, Hornix BE, Mantingh-Otter I, Zhou XD, Mari M, Reggiori F, Van Dam D, Eggen BJL, De Deyn PP, Boddeke E (2017) Progressive motor deficit is mediated by the denervation of neuromuscular junctions and axonal degeneration in transgenic mice expressing mutant (P301S) tau protein. J Alzheimers Dis 60:S41–S57. https://doi.org/10.3233/jad-161206

    Article  CAS  PubMed  Google Scholar 

  49. Yoshida H, Crowther RA, Goedert M (2002) Functional effects of tau gene mutations deltaN296 and N296H. J Neurochem 80:548–551

    Article  CAS  Google Scholar 

  50. Yoshida H, Goedert M (2006) Sequential phosphorylation of tau protein by cAMP-dependent protein kinase and SAPK4/p38delta or JNK2 in the presence of heparin generates the AT100 epitope. J Neurochem 99:154–164. https://doi.org/10.1111/j.1471-4159.2006.04052.x

    Article  CAS  PubMed  Google Scholar 

  51. Zhang W, Falcon B, Murzin AG, Fan J, Crowther RA, Goedert M, Scheres SHW (2019) Heparin-induced tau filaments are polymorphic and differ from those in Alzheimer's and Pick's diseases. eLife 8:e43584

    Article  Google Scholar 

Download references

Acknowledgements

We are grateful to Professor Y.A. Barde (Cardiff University) for providing the Tau knockout mouse line and Dr S. Gales (University of Cambridge) for work on antibody T49. We wish to thank staff at ARES for their help with animal husbandry, as well as the LMB biological services group for help with collection of animal tissues, especially C. Knox. The authors also wish to thank Dr P. Sarratt (University of Cambridge) for assistance with amino acid analysis of purified expressed Tau.

Funding

This work was supported by core funding from UK Medical Research Council (MRC) Grant MC_ U105184291 (to M.G.) and MRC grant G0600724 (to M.G.)

Author information

Author notes

  1. Jennifer A. Macdonald and Iraad F. Bronner contributed equally to this work.

Authors and Affiliations

  1. MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, CB2 0QH, UK

    Jennifer A. Macdonald, Lesley Drynan, Juan Fan, Isabelle Lavenir & Michel Goedert

  2. Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, CB10 1SA, UK

    Iraad F. Bronner

  3. AstraZeneca, Sir Aaron Klug Building, Granta Park, Cambridge, CB21 6GH, UK

    Graham Fraser

  4. Kymab, The Bennet Building, Babraham Research Campus, Cambridge, CB22 3AT, UK

    Annabelle Curry

Authors
  1. Jennifer A. Macdonald
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Iraad F. Bronner
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lesley Drynan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Juan Fan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Annabelle Curry
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Graham Fraser
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Isabelle Lavenir
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Michel Goedert
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

JM and MG designed experiments and drafted the manuscript. JM performed immunohistochemistry, quantitation of AT100 immunoreactivity, unbiased stereology, sarkosyl-insoluble extraction and immunoblotting on the P301S Tau and P301S Tau x mTau KO lines. IB did ELISA and maintained husbandry of Δ1-Δ3 lines. LD generated lines Δ1-Δ3. JF did recombinant Tau expression, purification, assembly assays and electron microscopy. AC assisted with tissue collection, immunohistochemistry, photographing of tissue sections, and AT100 immunoreactivity quantitation. GF did initial work with recombinant Tau, extracted sarkosyl-insoluble Tau and did immunoblotting on P301S Tau and Δ1-Δ3 lines. IL generated the P301S Tau x mTau KO line and helped with line husbandry.All authors read and approved the final manuscript.

Corresponding author

Correspondence to Michel Goedert.

Ethics declarations

Competing interest

The authors declare that they have no competing interests.

Ethics approval

All experiments were conducted in accordance with the UK Animals (Scientific Procedures) Act of 1986, with local ethical approval (Laboratory of Molecular Biology Animal Welfare and Ethical Review Body Committee).

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Macdonald, J.A., Bronner, I., Drynan, L. et al. Assembly of transgenic human P301S Tau is necessary for neurodegeneration in murine spinal cord. acta neuropathol commun 7, 44 (2019). https://doi.org/10.1186/s40478-019-0695-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1186/s40478-019-0695-5

Keywords

  • Tau assembly
  • Neurodegeneration
  • Tauopathy
  • β-Sheet structure
  • Tau filaments
  • Sarkosyl-insoluble tau

Acta Neuropathologica Communications

ISSN: 2051-5960

Contact us