Fig. 5

Relevance of murine Tau for assembly and neurodegeneration. The values of AT100 immunoreactivity (a) and motor neuron numbers (b) of homozygous 7–8-month-old mice transgenic for full-length human P301S Tau on a normal murine Tau background are taken as 100%. The results are expressed as means ± S.E.M. (5 animals/group). Two-tail unpaired student t-test revealed no significant difference (p = 0.78 and p = 0.85 respectively). c Survival of mice transgenic for full-length human P301S Tau compared to those on a murine Tau null background (20 animals/group). Two-tail unpaired student t-test revealed no significant difference (p = 0.09). n.s., not significant. d Immunoblots of sarkosyl-insoluble Tau from spinal cord of 7–8-month-old mice transgenic for full-length human P301S Tau, in presence (lane 1) or absence (lane 2) of murine Tau. Lane 3 shows lack of immunoreactivity in an age-matched wild-type mouse. Antibody AT100 is specific for Tau phosphorylated at T212/S214/T217 and identifies filamentous Tau. T49 recognises murine Tau, whereas HT7 is specific for human Tau