- Open Access
Systemic delivery of P42 peptide: a new weapon to fight Huntington’s disease
© Arribat et al.; licensee BioMed Central Ltd. 2014
- Received: 8 July 2014
- Accepted: 10 July 2014
- Published: 5 August 2014
In Huntington’s disease (HD), the ratio between normal and mutant Huntingtin (polyQ-hHtt) is crucial in the onset and progression of the disease. As a result, addition of normal Htt was shown to improve polyQ-hHtt-induced defects. Therefore, we recently identified, within human Htt, a 23aa peptide (P42) that prevents aggregation and polyQ-hHtt-induced phenotypes in HD Drosophila model. In this report, we evaluated the therapeutic potential of P42 in a mammalian model of the disease, R6/2 mice.
To this end, we developed an original strategy for P42 delivery, combining the properties of the cell penetrating peptide TAT from HIV with a nanostructure-based drug delivery system (Aonys® technology), to form a water-in-oil microemulsion (referred to as NP42T) allowing non-invasive per mucosal buccal/rectal administration of P42. Using MALDI Imaging Mass Spectrometry, we verified the correct targeting of NP42T into the brain, after per mucosal administration. We then evaluated the effects of NP42T in R6/2 mice. We found that P42 (and/or derivatives) are delivered into the brain and target most of the cells, including the neurons of the striatum. Buccal/rectal daily administrations of NP42T microemulsion allowed a clear improvement of behavioural HD-associated defects (foot-clasping, rotarod and body weights), and of several histological markers (aggregation, astrogliosis or ventricular areas) recorded on brain sections.
These data demonstrate that NP42T presents an unprecedented protective effect, and highlight a new therapeutic strategy for HD, associating an efficient peptide with a powerful delivery technology.
- Huntington’s disease
- Mouse model
Huntington’s Disease (HD) is a progressive neurodegenerative disorder caused by an abnormal expansion of polyQ (>35) domain in the N-terminus of the Huntingtin protein (Htt) . The worldwide prevalence of HD is 5–10 cases per 100,000 persons. Currently, only symptomatic treatments are available . One of the histopathological marker of HD is intranuclear aggregates formed by the polyQ-mutated Htt protein. These insoluble structures trap crucial proteins - and can physically block cellular traffic - and alter for instance the ubiquitin-proteasome degradation process ,. Altogether these defects finally contribute to the neurodegeneration occurring primarily in the striatum and involving other brain areas at later stages of the disease.
Although it is well established that the disease onset occurs as a consequence of expanded polyQ, other domains of Htt could also influence the pathogenicity of polyQ-hHtt. Indeed, the first 17 amino acids (N17 region) and the proline-rich region (PRR region), both flanking the polyQ stretch were found to influence aggregation ,.
Most research undertaken to find a cure for HD focus on the mutated allele (trying to reduce its expression) , or on the polyQ stretch that is responsible for aggregation, but that is also present in other neurodegenerative diseases ,. Our approach is based on the observation that the ratio between normal and mutant Huntingtin is a crucial factor in the onset and progression of the disease ,-. In a previous work, we confirmed the protective role of wild-type N-terminus Htt . We further screened human hHtt, for peptides able to prevent polyQ-hHtt aggregation in HeLa cells. To this end, 548aa N-terminal part of hHtt has been fragmented in shorter fragments according to domains lying in the protein. This step-by-step approach allowed the identification of a 23aa peptide (P42), lying in the N-terminus (position 480–502 aa) of normal human Htt (hHtt), able to prevent aggregation in cellulo in HeLa cells, and to retain protective properties on different phenotypes induced by the expression of the polyQ-Htt in vivo in a Drosophila model of HD (on eye degeneration, axonal trafficking and larval locomotion for instance) . P42 is able to prevent eye degeneration of flies expressing hHtt exon1 (covering 67aa, but not P42) with expanded polyQ . Interestingly, this effect of P42 is specific of HD, and not of other polyQ-induced diseases . This showed that P42 is able to act through hHtt 67 aa exon 1, independently to the polyQ stretch.
These previous data suggested that P42 could represent a new therapeutic agent to specifically target HD hallmarks. To test this possibility, we decided to analyse P42 protective properties in another model of HD, preferentially mammalian. In order to better quantify the protective power of P42, we choose to test R6/2 mice that express, as in the fly model, exon1 of human Huntingtin bearing 144 CAG repeats, and which do not contain the P42 domain ,. These mice exhibit early and severe behavioural phenotypes, associated to histological biomarkers mimicking HD. Symptoms start very early at week 5, leading to a premature death in weeks 12–15. In particular, R6/2 mice present neuroanatomical abnormalities including progressive reduction of the brain volume. They are also characterized by the presence of widespread nuclear inclusions of mutant polyQ-Htt in brain neurons. In addition, behavioural abnormalities are also present, including loss of coordination .
In order to test P42 in R6/2 mice, we developed a new peptide-based strategy. Peptides have many therapeutic applications, principally due to their efficiency, their selectivity and a high specificity compared to other molecules. Another important advantage concerns their degradation in amino acids that present a low risk of toxicity. Nevertheless, peptides present some limitations, related to their low capability to cross membrane barriers, short half-life, and difficulties of administration. To overcome these problems and optimize the delivery and action of P42, we adapted two complementary and original strategies. First, in order to cross cell membrane barriers efficiently and reach cytoplasmic and nucleic compartments, we fused P42 to the protein transduction domain of TAT from HIV. This 11aa Cell Penetrating Peptide (CPP) can be efficiently used as a vehicle to deliver fused peptides into living cells ,, via endocytosis . We first tested P42-TAT fusion peptide in HeLa cells for its abilities to transduce cells, and to rescue polyQ-hHtt aggregation. Secondly, in order to optimize the pharmacokinetic characteristics of P42 (serum half life and distribution profile) and to provide a non-invasive route for repetitive delivery of this fusion peptide, we used a novel water-in-oil microemulsion drug delivery vector named Aonys® ,. Aonys® provides a transmucosal (buccal) route of administration and enhances CNS penetration, which we verified by MALDI Imaging Mass Spectrometry.
The Aonys® formulated fusion peptide (named NP42T) represents a new product that combines for the first time the properties of a CPP, with a nanostructure-based drug delivery system. The aim of this study was to evaluate the efficacy of this new formulation of P42, NP42T, in the mammalian R6/2 HD mouse model.
Despite the high aggressiveness and the rapid onset of the phenotypes in the R6/2 model, buccal/rectal administration of NP42T significantly improved motor, histological and molecular phenotypes. These studies allowed to divert the natural properties of a Huntingtin domain P42 towards protection against aggregation and toxicity, which represents a new approach to fight against HD.
Peptide synthesis and formulation
To ensure P42 diffusion, we designed a fusion peptide where P42 was conjugated to the 11aa TAT Cell Penetrating Peptide (CPP). The peptide P42-TAT (AASSGVSTPGSAGHDIITEQPRS-GG-YGRKKRRQRRR) was synthetized and purified by Millegen company (France) with either a TAMRA (Tetra-Methyl-Rhodamine) tag at N-terminal position, or without any tag. NP42T is based on Aonys® drug delivery vector , where P42-TAT has been incorporated in a water-in-oil microemulsion. The lipid mixture Aonys® was prepared extemporaneously by weighing the lipid constituants. The appropriate amount of the peptide P42 powder (Millegen, purity >95%) was dissolved in MilliQ water. At a well-defined ratio, the P42TAT-Aonys® lipid monophasic microemulsion forms rapidly by short vortex mixing of the lipid mixture with the aqueous peptide solution.
Cell culture experiments and filter retardation assays
HeLa cells were grown and maintained in DMEM medium (Gibco) supplemented with 10% fetal calf serum and glutamine. HeLa cells were grown in 6 well plates, and pDdest53-GFP-hHtt171aa-136Q (0.75 μg) was transfected with JetPei reagent (Qbiogene). Equal amounts of pcDNA-Cherry-P42 or control vector were added for each transfection to level the total amount of plasmid DNA. To test the internalization of exogenous peptide, synthesized TAMRA-P42-TAT was added at the same time as pDest53-GFP-hHtt171aa-Q136 transfection, at different concentrations (0, 0.5, 1, 5, 10 or 20 μM). Cells were incubated with plasmid and peptide for 12 h. Then the medium was washed twice with PBS, the cells trypsinized, and grown on a coverslip with new DMEM medium for 6 h. This step was necessary to eliminate the peptide that crystallizes on the well. Eventually, cells were fixed with 4% paraformaldehyde and immunostained for microscopic analysis. To observe the internalization of the peptide TAMRA-P42-TAT, the signal was amplified by anti-TAMRA immunostaining (1:500- Invitrogen). Nuclei were counterstained with DAPI (4′, 6 diamino-2-phenylindole). The pictures were taken with a confocal laser scanning microscope model LSM780 (Carl Zeiss).
For filter retardation assays, protein extracts from HeLa cells were performed according to , with the following modifications: After transfection, HeLa cell pellets were treated by DNase and resuspended in 150 μl of 1% SDS and 50 mM DTT in PBS. The samples were boiled for 5 min, and diluted with 150 μl of 1% SDS. For each sample, two aliquots of 150 μl were filtered through a cellulose acetate membrane (0.2 μm pore size, Schleicher & Schuell), using a Bio-Rad dot-blot filtration unit. Membranes were then immunodetected with anti-GFP primary antibody (rabbit polyclonal (1:6000) Invitrogen), as indicated. The membranes have been secondary detected by HRP conjugated anti-rabbit antibody (1:50000-Jackson). Immunoreactive spots were detected with Immuno-Star WesternC kit (Bio-Rad), and quantifications have been performed with Image Lab3.0 software.
To test the stability of the peptide in the brain, single intracerebroventricular (ICV) injection of peptides was performed in the right ventricle of C57BL/6 mice as described in . 5 μg or 1 μg of TAMRA-P42-TAT were stereotaxically injected into the right lateral ventricle (coordinates 1 mm right lateral to bregma and 3 mm ventral to the skull surface) of C57BL/6 J mice using a 10 μl Hamilton syringe at a rate of 0.2 μl to 1 μl/min. As a control, saline was injected in the same volume and at the same rate. Mice were sacrificed at 6 h or 24 h by transcardiac perfusion of PFA.
For MALDI analysis, wild-type C57BL/6 J mice have been injected either by ICV (2.1 μg), or treated with empty NP, or with NP42T (210 μg) microemulsions. For NP and NP42T, four buccal and four rectal administrations (1 ml/kg) have been performed, spaced by 30 minutes to ensure mucosa internalization. Two mice have been treated for each condition and sacrificed 3 h after the injections.
Mass spectrometry and MALDI Imaging Mass spectrometry (IMS)
MALDI MS and MS/MS analysis: Spectra acquisition has been performed using the 4800 Plus MALDI TOF/TOFTM mass spectrometer (ABSciex) controlled by the 4000 Series ExplorerTM. MS spectra were acquired in a reflector positive mode (mass range: m/z 700–4000, laser intensity set at 50% and 3000 total laser shots per spectrum, MS resolution ≥ 12000–17000). MS/MS precursor was selected manually with an absolute mass window of 1 Da and each MS/MS spectrum is the result of 1000 total laser shots with a laser intensity set at 50% and a MS/MS resolution ≥ 2000–5000. The fragmentation was performed at collision energy of 2 kV and a collision air pressure set at 10−6 Torr.
Tissue preparation and MALDI imaging analysis: Frozen brain sections were cut in 15 μm slices using an HM 550 OVPD cryostat (Fisher Scientific, Illkirch, France), set at −20°C, mounted on ITO coated conductive glass slides and allowed to thaw and desiccate in a vacuum desiccator. Tissue sections were then coated manually with a 10 mg/ml α-cyano-4-hydroxycinnamic acid dissolved in 50% acetonitrile (ACN) and 0.1% trifluoroacetic acid (TFA).
MALDI imaging was performed using the 4800 Plus MALDI TOF/TOFTM Analyzer (AB Sciex). Image acquisition was achieved using the 4800 imaging tool software (MSI imaging). Imaging of brain sections was performed in a reflector positive mode, in the mass range of m/z 500–4000, with a resolution of 50 μm in a 100 × 100 μm raster, and laser intensity set at 80% of full laser intensity as selected within the 4000 Series ExplorerTM. At each position of the tissue section, an averaged mass spectrum is generated from 1000 consecutive laser shots.
The two dimensional ion image of the tissue section was analyzed using Tissue ViewTM software (Applied Biosystems/MDS analytical technologies). A colour scale representing signal intensity is exported from Tissue ViewTM software to provide semi quantitative information.
R6/2 transgenic and wild-type mice
R6/2 mouse model, based on the expression of the polyQ expanded human exon1 is a well-characterized model showing early and strong symptoms and leading to a premature death at 3 months of age -.
Initially, heterozygous R6/2 males were obtained from Jackson Laboratory (stock 006494). The crossing of these R6/2 males with C57BL/6-CBA F1 females generated the animals used in this study. The pups were weaned at week 3. After the weaning, the pups were housed in groups of mixed genotypes, and data were recorded for each mouse. Females issued from this crossing were treated from week 2 to week 11. Until the appearance of the tremor or clasping behaviour, transgenic mice could not be distinguished from normal mice in their home cage. The experimenters were blind to the genotype of the mice until the end of the protocol. The mice were housed fifteen per cage under standard conditions with ad libitum access to water and food. At the end of the protocol, mice were genotyped by PCR using tail-tip DNA as described . A real time PCR was performed with the following primers HDexon1 Forward : 5′- CGG CTG AGG CAG CAG CGG CTG T-3′, and HDexon1-Reverse : 5′- GCA GCA GCA GCA GCA ACA GCC GCC ACC GCC3′. At week 11, mice were sacrificed and tissues fixed for histology by transcardiac perfusion of 4% PFA in PBS.
Procedures for the care and treatment of animals were carried out according to CNRS guidelines, and the experimental protocol was approved by the institutional animal care committee of the Institut de Génomique Fonctionnelle, in accordance with directives of the French Ministry of Agriculture (agreement number: D34-172-13).
For animal experimentation, a cohort of 30 females was divided into four groups (WT treated with empty NP (n = 5); WT treated with NP42T (n = 8); R6/2 treated with empty NP (n = 8); R6/2 treated with NP42T (n = 9)). Administrations were performed five days a week, at the same time of the day, starting in 2-week-old mice. The treatment was performed until the mice were sacrificed (at week 11). NP42T peptide (600 μg/ml/kg) was administrated twice a day per mucosa, with two buccal injections until the mice were 4 week old, and via rectal and buccal injections for older animals older.
Behavioural tests of mice
Body weights of the R6/2 transgenic and wild-type mice were recorded weekly at the same time of the day, from the week 2 .
Motor performance was assessed using an accelerating rotarod (Stoelting, Ugo Basile, Biological Research apparatus, Varese, Italy), at weeks 6, 8, and 10 . On the first day, the mice were trained with a trial at an accelerating velocity from 4.5 to 40 rpm. Subsequently, two trials were performed during two consecutive days. In each trial, mice were placed onto the rotarod at a constant speed of 4.5 rpm for 5 seconds, which then accelerated at a constant rate up until 40 rpm, for a maximum of 5 min. The daily trial was composed of two sessions of 5 min separated by 20 min. The latency to fall from the rotarod was recorded every day for each mouse, and the average of two trials was used for statistical analysis.
For the clasping test, mice were suspended by the tail for 30 sec. The frequency and the duration of the foot-clasping posture were scored twice a week . The average of two trials was used for statistical analysis. The tests were performed at 7, 9, and 11 weeks of age.
For tests with a single time point of data acquired, either an unpaired t- test or one way ANOVA, followed by Fisher’s LSD post hoc test was used, when necessary. Otherwise two-way ANOVA ordinary with Time and Group or Genotype and Treatment as between-subject factors was applied, followed by Bonferroni’s post-hoc analysis for multiple comparisons. In all cases, significance threshold was set at p < 0.05. Prism 6.0 software was used to perform statistical analyses.
Coronal brain sections of 50 μm thickness were cut with an electronic microtome (Microm HM 650 V). The slides from bregma −0.60 to bregma −0.80 mm were used for Hematoxylin/eosin staining and immunostained with monoclonal antibody anti-Huntingtin (MAB5374 – Millipore – 1:1000) or polyclonal anti-GFAP (AB5804 – Millipore – 1:1000 ). For each animal, systematic pictures were taken with the slide scanner Hamamatsu nanozoomer. The areas of lateral ventricles and cortex thickness were measured with NDP software (Nanozoomer Digital Pathology Virtual SlideViewer). Quantifications of aggregates number and size, or GFAP signal, were scored with ImageJ software from 256 μm × 256 μm images. Representative pictures for these immunostainings obtained with the confocal laser scanning microscope model LSM780 (Carl Zeiss) are shown.
TAMRA-P42-TAT injected mice were also immunostained with rabbit monoclonal anti-DARPP32 (MAb2306 - Cell Signaling Technology - 1: 1600), secondary detected by Alexa 488 anti-rabbit (1: 2000) to label the striatum, and with mouse monoclonal anti-NeuN (MAB377 – Millipore - 1: 1000), secondary detected by Dye light blue anti-mouse (1: 500), as a neuronal marker.
Construction of NP42T, a new tool against HD
Intracellular delivery of P42 peptide when conjugated with TAT
Pharmacokinetics and targeting of P42-TAT into the brain
One challenge to use P42-TAT at therapeutic ends was to find a non-invasive route of delivery, such as buccal administration and to improve P42-TAT bioavailability and uptake into the brain. To this end, we developed a nanostructure-based drug delivery system (Aonys® technology), to form a water-in-oil microemulsion (referred to as NP42T) allowing per mucosal buccal/rectal administration, and compared this mode of delivery to intra-cerebroventricular (ICV) injection.
We hypothesized that some of these degradation products could be present in the brain in addition to the parent P42-TAT peptide. Thus, we analysed the fate of P42-TAT when administered into the brain of wild-type C57BL/6 J mice by ICV injection or by buccal/rectal administration of NP42T encapsulated into Aonys® microemulsion. Since the brain corresponds to approximately 1% of the mouse total weight, we used 100 times more NP42T than for ICV injection.
We used MALDI Imaging Mass Spectrometry , whereby the different compounds present in brain sections can be detected simultaneously without further labelling. Using this technology, intact P42-TAT peptide and its degradation products were sought into mouse brain slices three hours after injection. An analysis of the distribution of 30 putative peptide derivatives was performed on brain sections of treated mice. This extensive analysis allowed identifying P42-TAT derivatives only detected in P42-TAT treated mice (Figure 2B). For instance one peptide (m/z 1801.84) is detected in brain extracts after 1 or 3 hours incubation (Figure 2A). A MS/MS characterisation of this peptide confirmed that it corresponds to a part of injected P42-TAT: −GSAGHDIITEQPRSGGYG (Additional file 1: Figure S1).
These data confirm that P42-TAT is able to reach the brain when administrated via Aonys® microemulsion.
Targeting of the peptide in neuronal cells of the striatum
Altogether these data showed that P42-TAT has the ability to diffuse into the brain in the different cell layers, including the striatum. It is also able to reach the different subcellular structures (nucleus and cytoplasm) of the neurons. We also showed that delivering the peptide using Aonys® water-in-oil microemulsion via buccal/rectal mucosa guarantees the peptide to reach different parts of the brain, as efficiently as by intracerebroventricular injection.
Treatment of R6/2 mice by NP42T
In order to confirm in vivo the efficiency of P42 combination with TAT and the Aonys® microemulsion, we tested the effect of NP42T in R6/2 mouse model of HD. R6/2 mice are a particularly suitable model for two reasons: first, P42 protective properties have been tested in a Drosophila model, expressing exon1 , and it was important to test this peptide on a mammalian equivalent model. Second, in R6/2 mice, the P42 domain is lacking, allowing to precisely seizing the protective properties of exogenously administered P42.
R6/2 mice suffer from a progressive decrease in body weight and resting tremor, movements described as resembling chorea ,. Administrations of P42-TAT/Aonys® (NP42T) or empty (NP) microemulsions via buccal and/or rectal mucosa were performed daily, 5 days per week, in R6/2 and in wild-type C57BL/6 J mice. NP42T was administrated between week 2 and week 11: twice orally during the first two weeks and orally plus rectally from week 4 to week 11. Several polyQ-hHtt behavioural associated defects were analysed (foot-clasping, rotarod or body weights), and several markers were evaluated on brain sections (size of ventricles, aggregation and astrogliosis).
NP42T reduces behavioural defects of R6/2 mice
To evaluate the therapeutic potential of P42-TAT, we analysed different behaviours.
When suspended by the tail, normal mice spread their four limbs, whereas R6/2 mice clasp their hind- and forelimbs tightly against their thorax and abdomen (Figure 4A, A’). Six-week-old R6/2 animals begin to exhibit this clasping reflex, which can be easily used to examine novel treatments. We first assessed the effect of buccal mucosa administration of NP42T on foot clasping (Figure 4B). The duration of clasping posture over a 30 seconds period increased in placebo-treated R6/2 mice, with a mean score of 13.7 ± 4.27 s in 11 week-old animals. NP42T treatment reduced massively this defect, with a 10 fold better score, showing a clasping duration of 1.35 ± 0.71 s in 11 week-old R6/2 mice (Figure 4B), which was indistinguishable from the wild-type score. Therefore, NP42T treatment clearly prevents hindleg reflex with a durable effect.
These results show that NP42T is able to significantly rescue behavioural dysfunctions of R6/2 when using chronic administrations.
NP42T impacts peripheral phenotypes
The Aonys® formulation technology allows the delivery of the therapeutic peptide NP42T via the lymphatic system and general circulation to the central nervous system as well as to other organs. Indeed, peripheral symptoms have an important impact on HD patient survival. Cardiac dysfunctions and diabetic anomalies contribute to HD patient mortality . Among peripheral phenotypes, one of the major defects leads to an alteration of the body weight evolution. Indeed, R6/2 mice begin to lose weight at week 8 (Figure 4D). Between week 8 and 11, wild-type cohorts exhibited a body weight gain of 5.47% ± 1.1. During the same time period, placebo-treated R6/2 animals showed a weight loss of −3.39% ± 2.1, whereas NP42T-treated R6/2 mice exhibited an increase of 3.92% ± 1.61 of their mean body weight. These results showed that NP42T was able to restore the body mass curve (Figure 4D).
NP42T reduces histopathological markers of HD in R6/2 mice brains
Altogether, these data confirm the powerful protective effect of P42 in vivo. The original delivery method based on CPP and a novel microemulsion formulation allowed the peptide to efficiently cross the blood–brain-barrier and its wide spread into the brain, with an efficient rescue on most of the associated HD-phenotypes that were tested.
Despite of recent progresses, HD remains a fatal neurodegenerative disorder. In this context, the identification of P42 represents a new therapeutic avenue. P42 is a 23aa peptide that lays in the N-terminal sequence of Huntingtin. We previously showed that this peptide is able to prevent aggregation of mutant Huntingtin in HeLa cells. Its therapeutic potential was also confirmed in vivo in Drosophila models of HD, on different phenotypes specifically induced by the expression of polyQ-hHtt . To confirm P42 protective properties, we choose in this report to test P42 in a mammalian animal model.
The use of P42 as a drug in mammals requires the ability to deliver this peptide into the brain. Indeed poor absorption and high enzymatic degradation of peptides are critical for the use of such tools. The need for a chronic treatment requires the development of a non-invasive method of administration, in order to ensure patient compliance. To this end, we joined for the first time two original strategies to provide an efficient distribution of P42 until its targeting into the cells, by using i) Aonys®, a water-in-oil microemulsion , and ii) a fusion peptide composed of TAT and its cargo P42. We verified that the presence of TAT enhances the diffusion of P42 into a large number of cells. We could also verify that the fusion peptide P42-TAT has kept its protective properties and could diffuse into the cells, targeting the different regions of the brain and the different subcellular compartments. This diffusion of P42-TAT will help to provide better efficacy, preventing aggregate intercellular spreading for instance . In a second step, in order to enhance the low stability and half time of P42-TAT and to enhance its residence time in the CNS, we used the Aonys® microemulsion formulation technology (Medesis-Pharma) to deliver the fusion peptide. P42-TAT was formulated in the water-in-oil microemulsion, allowing per mucosal administrations and providing efficient delivery. Therefore, the formulation named NP42T took the advantages of these two delivery technologies. The buccal and rectal administration of such a formulation represents the most appropriate and non-invasive route for chronic peptide delivery. Using this technique, we verified that P42-TAT is targeted to the brain as efficiently as when provided directly by intra-cerebroventricular injection. We also identified that P42 derivatives reside several hours in the brain.
Per mucosal administration of NP42T in R6/2 mice allowed early treatment starting at the second week. Thus, we ensured the delivery of the peptide before the first defects appearance. The treatment of R6/2 mice with NP42T improved most of the HD symptoms when administrated pre-symptomatically, delaying for instance the motor impairment observed on rotarod test by around 4 weeks. The benefits observed on the foot clasping phenotypes were also notable until the animals were sacrificed at week 11. For pathological markers, such as aggregation or astrogliosis, the treatment reduced their intensity. Notably, NP42T decreased the number of the aggregates, but not their size. One hypothesis is that by targeting hHtt exon1 , P42 might interfere on both the nucleation and aggregation processes, which might prevent new aggregates to form. Note that because of exon1 self-interactions , undoing aggregates is more difficult, which might explain that when formed, the size of the aggregates keeps unchanged. However, blocking the formation of new aggregates may allow the neurons to eliminate the preexisting inclusions and the soluble mutant Htt in excess to recover normal functioning, as previously mentioned . Even though the exact role of aggregates and their involvement in cell toxicity remain controversial , we observed that the lower number of aggregates is correlated with a recovery of different phenotypes, such as motor performances for instance.
This raises the question concerning the efficiency of P42 for post-symptomatic treatment. In 6 week-old R6/2 mice, most of the symptoms are already developed . In these conditions, a late treatment revealed protective effects on a range of phenotypes, but to a lower extent (our unpublished results). This suggests that P42 might not only target aggregate formation but could also interfere on polyQ-Htt toxicity at other levels, which might delay the progression of the disease.
In humans, the possibility of a pre-symptomatic test, preceded by genetic counseling and carried out in accordance with international recommendations, allows risky subjects to assess their genetic status and to predict whether a subject will develop HD, long before becoming symptomatic . Pre-symptomatic phase is a unique opportunity for early intervention and neuroprotection, in which our peptide could be proposed.
The microemulsion delivery mechanism is likely to mediate a systemic distribution of P42-TAT in the whole organism. Even if the striatum and the cortex are the most affected regions in HD, peripheral symptoms play an important part in the quality of life, and eventually the mortality of HD patients . For instance, cardiovascular defects are responsible for the death in 30% of the patients . For these reasons, whole body delivery of therapeutic molecules seems more appropriate than a local administration (such as viral delivery in a limited region). Indeed, the effect of NP42T on body weight could be explained by more peripheral benefits. We investigated other peripheral phenotypes such as heart mass, heart ventricle size and glycaemia, but no clear variations were observed between wild-type and R6/2 non-treated mice during the time of our study (data not shown). The relatively short timing of the study (from 2 to 11 weeks) and the use of a softer stock of R6/2 mice could explain that we did not notice these peripheral phenotypes previously described in other reports . Nevertheless, the effect of NP42T on body weight evolution might be representative of an action on muscle mass, metabolism, and food intake. Note that no toxicity of P42-TAT has been noticed during this study, since NP42T-treated wild-type mice developed normally, and IC50 value on HeLa cell survival (MTT test) could not be measured even in the presence of a large excess of peptide. Moreover the number of neurons, quantified after NeuN-immunostaining, was identical in brains of wild-type mice treated by NP42T or empty NP. Finally neither astrogliosis (detected by GFAP-immunostaining- Figure 6), nor microglia activation (detected by IBA1-immunostaining- data not shown) were identified in NP42T-treated wild-type brains.
The efficient rescue supported by P42 administration represents one of the best therapeutic effects described so far. Therapeutic research currently targets mutant Htt at transcriptional or post-translational level. The recent works in RNA interference or ASO strategies  attempt to reduce the expression of the mutant transcript. Despite recent progress, the limitation of this approach lays in the effects on the wild-type allele and the aggravation of the loss of function effects. Biochemical molecules and intrabodies have also been designed against the protein, in order to target abnormal interaction and aggregation processes. In particular, a range of molecules targeting directly the polyQ region of the mutant Huntingtin, have been designed. For instance, the peptide QBP1 and the molecule C2-8 bind the polyQ region and reduce aggregation. These factors drove clear effects in cell models ,, but rather mild effects in R6/2 mouse model ,. The combination of TAT with transmucosal microemulsion delivery allowed us to use particularly low doses of P42 (120 ug/week of peptide in adult mice), comparative to what are usually used. In comparison, injection of 500 μg-2 mg/week of QBP1, fused to TAT, had no effect on aggregation process in R6/2 mice, and only a slight effect on body weight . Among the drugs that target the polyQ-hHtt, C2-8 had the ability to also reduce the aggregation process and to improve rotarod performances of R6/2 mice, but this was obtained only in presence of high doses of products (100 or 200 mg/kg/12 h) . The direct targeting of polyQ region might also provide unspecific action, especially if using high doses. Indeed, a wide range of proteins, including transcription factors, physiologically contain a polyQ stretch, which is crucial for their activity, suggesting that chronic treatment might lead to high toxicity. Hence, new strategies that specifically target Huntingtin regions flanking the polyQ have been tested. C4 and VL12.3 intrabodies, designed against the N17 region to inhibit nucleation and aggregation, showed encouraging effects in cell model ,, but only moderate improvements of R6/2 mice . Hence, the performances of NP42T reported here in R6/2 mice should be greater in other HD models. In particular, P42 effects on lifespan are partial , and should be evaluated on less aggressive line.
In summary, P42 combines the specificity of intrabodies targeting flanking regions, but resorts to natural molecular mechanisms normally used by the cells. In addition, the clear dose-effect of P42-TAT that we identified in cell cultures, suggests that the doses that we administered could be enhanced to provide greater benefit.
To conclude, this report highlights a clear therapeutic potential of P42, a 23aa peptide, localized in endogenous Huntingtin protein . The action of this molecule was potentiated through the combination of transmucosal microemulsion delivery and CPP support. Such a strategy could be applied more generally to other disorders, requiring daily treatments. In early pre-symptomatic treatment, NP42T is protective both on aggregation and on different behavioural or physiological phenotypes. Because HD is a genetic disorder, early screening for patients at risk would enable pre-symptomatic treatment. Later post-symptomatic administration of P42 in R6/2 mice might also help to delay the progression of the disease, but need to be tested further. Nevertheless our data showed that P42 is clearly able to prevent the appearance of symptoms, and to impede the progression of the disease. To enhance the recovery of cell activity, a treatment based on P42 should be combined with other strategies to stimulate autophagic process or proteasome activity for instance ,, but also in bitherapies, associating for instance the lithium that was also shown to be protective . Because of the aggressiveness of HD, it seems important to envisage a synergic use of the different therapeutic ways. In this context, P42 might represent one of the most powerful tools to fight efficiently against mutant Huntingtin toxicity.
We wish to thank Cecilia Marelli, Peter Jackson and Elsa Compte for discussions during this work. Christelle Cadilhac and Emmanuel Valjent for technical advice on mice. Federica Bertaso for helpful discussions on mouse work before the start of this study.
YA was supported by a fellowship from the French Ministry of Research and Technology. This work has been supported by the CNRS, “soutien au transfert” grant, UM2 contribution, and ANR Emergence (ANR-12-EMMA-0013).
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