- Case report
- Open Access
Two cases of multinodular and vacuolating neuronal tumour
© Bodi et al.; licensee BioMed Central Ltd. 2014
- Received: 3 September 2013
- Accepted: 13 November 2013
- Published: 20 January 2014
An unusual multinodular and vacuolating neuronal tumour (MVNT) has been described in the cerebral hemispheres of ten patients with adult-onset seizures. We report the findings in two cases with similar features, a surgical resection and the other an autopsy specimen.
Case 1, a 34-year-old female, underwent surgical resection for a multinodular non-enhancing frontal white matter lesion causing intractable epilepsy. Case 2, presented with motor neurone disease (MND) at the age of 71 and MRI scanning revealed extensive multinodular non-enhancing white matter lesions in the temporal lobe. There was no history of epilepsy and post mortem histology confirmed MND.
Macroscopically multiple small grey well-formed, discrete and coalescent nodules were seen in the deep cortex and subcortical white matter. On histology, mature-looking neurons with large cytoplasmic vacuoles were distributed in a fibrillary background, where vacuoles were also noted. In the resected tumour scattered oligodendroglia-like cells were present. No ganglion cells were seen. The vacuolated cells exhibited immunopositivity for synaptophysin, HuC/HuD and p62 but were negative for NeuN, neurofilament, GFAP, IDH1, nestin and CD34. Electron microscopy showed non-membrane bound cytoplasmic vacuoles in the neurons and in some neuronal processes. The seizures recurred in Case 1.
Some clinicopathological features of this lesion suggest a possible relationship with dysembryoplastic neuroepithelial tumour (DNT) although the morphological features are not typical of DNT. Case 2 demonstrates that MVNT may remain asymptomatic.
- Dysembryoplastic neuroepithelial tumour
- Brain tumour
The improving imaging techniques and the availability of surgery for the treatment of chronic, drug-resistant epilepsy have lead to the recognition of indolent brain tumours that appear to be largely restricted to this clinical setting. Many are of glio-neuronal lineage of which dysembryoplastic neuroepithelial tumour (DNT) and ganglioglioma are to be the most frequent . However, there still appears to a lack of consensus on the criteria to diagnose these lesions, and there continue to be cases where the appearances do not conform to established criteria for diagnosis and may remain unclassifiable. Most epileptogenic neuroepithelial tumours present in childhood but may not get surgical treatment until adulthood due to their indolent clinical behaviour. Recently, a non-neurocytic, purely neuronal lesion affecting adults with epilepsy has been described, in which there was striking vacuolation of the neuronal cytoplasm, referred to as multinodular and vacuolating neuronal tumour (MVNT) . We report two cases with similar histological features, one from a surgical resection to treat chronic seizures, and the other from an autopsy specimen without a history of epilepsy, with MRIs in both cases suggesting a diagnosis of DNT.
Materials and methods
The surgically resected material (Case 1) was fixed in buffered formalin for 24–36 hours. All sampled tissue blocks were processed and embedded in paraffin wax and stained by conventional haematoxylin and eosin (H&E). Selected blocks showing histological abnormalities were further stained with Luxol-fast blue/Nissl (LFB/N) and PAS-D. Immunohistochemistry was carried out with antibodies against neuronal nuclear protein (NeuN), neuronal protein HuC/HuD (HuC/HuD), pan-neurofilament (pan-NF), neurofilament 200 KD (NF200 KD), synaptophysin, chromogranin, glial fibrillary acidic protein (GFAP), nestin, CD3, CD45, CD68, CD34, human leukocyte antigen-DR (HLA-DR), isocitrate dehydrogenase1 (IDH1) R132H, calbindin, parvalbumin, calretinin, Ankyrin-G, p62 and Ki67.
The brain and the spinal cord (Case 2) were consented to research and donated to the MRC London Neurodegenerative Disease Brain Bank at King’s College Hospital. After fixation in buffered formalin for 12 weeks, the whole brain and the spinal cord were sampled as per our departmental protocol for MND. All sampled blocks were stained with H&E and selected blocks were stained with LFB/N, p62, TDP-43, tau, CD34, CD68, PAS, Sudan black, Nestin, NeuN, pan-NF, NF200 KD, Ki67, CD68, HLA-DR, CD45, CD3, α-synuclein, amyloid precursor protein (APP).
The sources of the antibodies used, and respective pre-treatment and dilution
DIA H09 L
Santa Cruz biotech
BD Transduction labs
BD Transduction labs
Cosmo Bio Ltd.
Electron microscopy was performed on material re-embedded from paraffin block in Case 1 and from formalin fixed wet tissue in Case 2 .
Genomic DNA was extracted from formalin fixed paraffin embededd tissue using the QIAamp DNA FFPE Tissue kit (Qiagen GmbH - Hilden, Germany). Bisulphite conversion was performed using an EpiTect kit (Qiagen GmbH - Hilden, Germany). MGMT promoter methylation status and BRAF V600 mutation were determined using the therascreen MGMT Pyro Kit and therascreen BRAF Pyro Kit respectively using a Q24 MDx Pyrosequencer (Qiagen GmbH - Hilden, Germany). Sequencing of IDH1 and IDH2 was also performed by pyrosequencing methods [4, 5].
The specimen consisted of 4 fragments measuring 30 × 30 × 20 mm, 20 × 10 × 5 mm, 20 × 10 × 3, and 15 × 10 × 3 mm. On slicing at 5 mm intervals, two grey nodules of 2 -3 mm were identified in the subcortical white matter (Figure 1C).
The formalin fixed brain weight was 1198 g. Macroscopic examination of the brain revealed no significant cortical atrophy but the spinal cord showed mild atrophy of the anterior motor roots, consistent with MND. Coronal slices revealed widespread multiple discrete grey, plaque-like nodules measuring 1-3 mm scattered in the white matter of the posterior part of the left temporal lobe (Figure 2C).
Case 2 showed typical histological features of MND in the brain and spinal cord.
In Case 2, the immunophenotype confirmed the diagnosis of MND with limbic and mild neocortical TDP-43 pathology. Occasional TDP43 positive oligodendroglial inclusions were seen within the lesions, as frequently as in the rest of the white matter.
Molecular genetic tests
There was no evidence of mutations of BRAF V600E, IDH1 and IDH2 in either case. MGMT promoter showed borderline methylation in Case 1, while Case 2 was unmethylated.
We have described two cases, a surgical resection and the other an autopsy specimen, with histological features similar to those recently described as MVNT by Huse et al. . The radiological features were also similar, showing lesions with T2 signal hyperintensity and increased signal in FLAIR in the subcortical white matter; being subtle in Case 1 and extensively involving the posterior part of the temporal lobe in Case 2. Both our cases were favoured to be DNT by the radiologists as having absence of mass effect and no surrounding oedema . Multinodularity was demonstrated both radiologically and by macroscopic examination in our cases. The discrete and coalescing grey nodules abutted on the deep cortex although most lesions were subcortical. This type of localisation may also occur in DNT but appears also to be characteristic of MVNT .
Until the neuronal nature of the strikingly vacuolated cells could be established, the uniform greyness of the lesions and the loss of myelin staining raised the differential diagnosis of demyelinating disease. In Case 2 the nodules were numerous but there were none in a periventricular location as usually observed in multiple sclerosis. There was prominent microglial activation with some foamy macrophages which sharply demarcated the abnormal areas from the surrounding white matter. Microglial marker upregulation has been found in tumours from patients with epilepsy, such as diffuse and nodular DNT, ganglioglioma and pleomorphic xanthoastrocytoma, and has also been described in focal cortical dysplasia (FCD) [8, 9]. However, there was no perivascular lymphocytic inflammatory reaction and the vacuolated neurons were clearly negative with microglial/macrophage markers. Moreover, some myelinated axons were still noticeable within the nodules. It is possible that the vacuolation, affecting not only the abnormal neurons but the neuropil as well, may be responsible for the hypomyelination observed in the nodules. There was no indication of any metabolic disorder or ion imbalance in any of our cases to account for a non-inflammatory type of demyelination.
The vacuolated cells failed to express NeuN (available in Case 1). It appeared to label only the unaltered normal mature neurons of the white matter but not the vacuolated neurons. There was also no chromogranin or neurofilament positivity in the cytoplasm of those cells but axonal immunoreactivity with neurofilament was detected in the axons running through the lesion. We were able to confirm consistent labelling of the vacuolated cells by HuC/HuD as reported by Huse et al. . In addition we also noted immunoreactivity in small round nuclei being present not only in the lesion but also in the normal white matter, suggesting labelling of some of the normal oligodendroglial cells. Previous immunohistochemistry studies with anti-HuC/HuD described positivity, in addition to small cell carcinoma of lung, in neoplasms of differentiated neuronal type and embryonal tumours with neuronogenic potential but no glial tumours apart from occasional oligodendrogliomas [10–12]. Immunolabelling of normal oligodendroglial cells has not been reported previously and this may be attributed to more aggressive antigen retrieval method used by us. We found strong synaptophysin expression in Case 1 but this was weaker in Case 2, probably due to prolonged fixation in autopsy material. The synaptophysin expression was variable in the series of Huse et al.; three cases were positive, three cases were negative and four cases had focal weak labelling. We also demonstrated increased somatic Ankyrin-G expression of the vacuolated neurons; however, the AIS was not labelled in neurons within the lesions. Changes in the structure of the AIS may result in modifications of the excitability of these neurons, as described previously [6, 13]. We also found increased p62 immunoreactivity within the abnormal. Although the p62 labelling is not specific, defective autophagy may be suspected in the vacuolated neurons.
Electron microscopy confirmed the neuronal nature of the vacuolated cells and the presence of a neuropil, which also showed some vacuolation. The cause of the vacuolation of neurons could not be established nor could it be related to a specific cell organelle. There was no accumulated material. A selective artefact affecting the neurons within the lesion is unlikely.
The question remains unanswered as to whether the nodules of markedly vacuolated neurons represent definite neoplastic transformation or aberrant development. Nodular heterotopia has similar naked eye appearances although the nodules are usually more well demarcated from the cortex and do not show coalescence [14, 15]. However, vacuolation is not a feature of heterotopia as they tend to show normal composition of neurons and neuropil without significant reactive astrocytic or microglial reaction [14, 15]. Some nodular heterotopias are associated with cortical dysplasia in the overlying cortex , but there was no cortical dysplasia observed in our cases. The vacuolated neurons failed to express NeuN which is preserved in heterotopic neurons and there was abnormal immunomarker expression in the vacuolated neurons compared to normal cortical neurons [14, 15]. Reactive astrocytosis and microglial cell activation, which was prominent within the lesions in both our cases, also not a feature of nodular heterotopias. The reactive changes can be explained as the consequence of chronic seizures in Case 1, but there were no seizures in Case 2. The presence of some foamy macrophages within the nodules, a few with myelin debris, is also suggestive of an acquired lesion.
Most importantly, the differential diagnosis includes the well known glioneuronal neoplastic lesions. We saw no ganglion cell morphology or atypia characteristic of ganglioglioma and gangliocytoma. Although vacuolar alteration is not uncommon in ganglion cell tumours, those are rarely and inconsistently seen in the ganglion cell component, and more likely to manifest as microcystic or myxoid change. There was also no typical accompanying glial component in these lesions, such as Rosenthal fibres, eosinophilic granular bodies, microcalcification or lymphocytic inflammatory reaction [16–18]. The lack of neurofilament and chromogranin immunoreactivity would also speak against ganglioglioma. Huse et al. described focal immunoexpression of CD34 in 7 out of 10 cases in ramified processes of small cells but no immunoreactivity in the vacuolated neurons. Such CD34 immunoreactive cells have been found in various epileptogenic lesions, including focal cortical dysplasia type IIB, and frequently colonize the cerebral cortex adjoining gangliogliomas, pleomorphic xanthoastrocytomas, observed in association with complex and diffuse type of DNT and even detected in high grade gliomas [8, 19, 20]. We did not observe CD34 immunoreactivity in our two cases.
Molecular genetic testing showed no mutation of BRAF V600E, which may be found up to 50% of gangliogliomas and pleomorphic xanthoastrocytomas , in either of our 2 cases or in 4 cases tested by Huse et al. Nevertheless, Huse et al. demonstrated a point mutation involving MEK 1 (MAP 2 K1) in 1 out of 8 tested cases.
The presence of an oligodendroglial/OLC component is suspected in this lesion. Huse et al. described OLIG2 expression in all of their lesions . We also detected increased a number of oligodendroglial-like cells and oligodendrocytes within and sometimes at the border of the nodules. The OLC is a characteristic cell component of DNT and is essential for the diagnosis [22, 23]. There was no evidence of so-called “specific glioneuronal lesion” in our cases, but this is absent in the diffuse DNT, and may not be seen in the complex multinodular DNT [8, 24]. However, the predominantly neuronal composition of these lesions and the striking cytoplasmic vacuolation has not been reported so far in DNT.
MVNT was reported as a lesion associated with adult onset seizures. Of the ten cases reported by Huse et al., five cases had adult onset epilepsy, most with a short history of seizures or episodic confusion (between 1 to 5 years) . Three patients had only a single episode and one had a possible episode, which prompted neuroimaging and the discovery of the lesions. The last, a 54 year-old man, presented with seizures for over 40 years starting in childhood. The latter is similar to our Case 1 (seizure onset at 8, epilepsy for 28 years). Our second case, on the other hand, an elderly patient with MND and no history of seizures had extensive temporal lobe lesions which were discovered only by routine MRI scanning as per the MND protocol. While it is possible that mild symptoms went unnoticed in the face of a severe neurological illness, it is likely that other non-seizure associated incidental cases may come to light in the future.
The biological behaviour of MVNT appears to be benign, similar to DNT. Regardless of the extent of excision, no tumour recurrence was noted in the 8 cases available for follow up in the series of Huse et al. . Our cases also did not show re-growth or increase in size of the lesion on follow-up images. Only one out 8 patients of Huse et al. remained symptomatic after subtotal lesion removal, suffering seizures and cognitive disturbances. Although the frequency of seizures decreased after surgical resection (Case 1), our patient did not become seizure-free.
Many of the tumour-like glioneuronal lesions associated with chronic epilepsy appear to be clearly neoplastic, others continue to confound, straddling the boundary between malformation and neoplasm. The ten MVNT cases recently reported by Huse et al. were named as tumour but share features with malformation. On the other hand, this type of lesion shows similarities at least in behaviour with DNT and it is possible that the MVNT may even form part of the spectrum of DNT. MVNT may also present as an incidental lesion without seizures.
Written informed consent for publication were obtained for case report using accompanying MRI and histological images without identifiable patient details.
We thank the donor and her family for the donation of brain and spinal cord tissue to the Medical Research Council London Neurodegenerative Diseases Brain Bank. We also thank the staff of the Clinical Neuropathology Department, King’s College Hospital.
- Thom M, Blumcke I, Aronica E: Long-term epilepsy-associated tumors. Brain Pathol 2012, 22: 350–379. 10.1111/j.1750-3639.2012.00582.xView ArticlePubMedGoogle Scholar
- Huse JT, Edgar M, Halliday J, Mikolaenko I, Lavi E, Rosenblum MK: Multinodular and vacuolating neuronal tumors of the cerebrum: 10 cases of a distinctive seizure-associated lesion. Brain Pathol 2013, 23: 515–524. 10.1111/bpa.12035View ArticlePubMedGoogle Scholar
- van den Bergh Weerman MA, Dingemans KP: Rapid deparaffinization for electron microscopy. Ultrastruct Pathol 1984, 7: 55–57. 10.3109/01913128409141854View ArticlePubMedGoogle Scholar
- Setty P, Hammes J, Rothamel T, Vladimirova V, Kramm CM, Pietsch T, Waha A: A pyrosequencing-based assay for the rapid detection of IDH1 mutations in clinical samples. J Mol Diagn 2010, 12: 750–756. 10.2353/jmoldx.2010.090237View ArticlePubMedPubMed CentralGoogle Scholar
- Song X, Andrew Allen R, Terence Dunn S, Fung KM, Farmer P, Gandhi S, Ranjan T, Demopoulos A, Symons M, Schulder M, Li JY: Glioblastoma with PNET-like components has a higher frequency of isocitrate dehydrogenase 1 (IDH1) mutation and likely a better prognosis than primary glioblastoma. Int J Clin Exp Pathol 2011, 4: 651–660.PubMedPubMed CentralGoogle Scholar
- Grubb MS, Burrone J: Activity-dependent relocation of the axon initial segment fine-tunes neuronal excitability. Nature 2010, 465: 1070–1074. 10.1038/nature09160View ArticlePubMedPubMed CentralGoogle Scholar
- Stanescu Cosson R, Varlet P, Beuvon F, Daumas Duport C, Devaux B, Chassoux F, Fredy D, Meder JF: Dysembryoplastic neuroepithelial tumors: CT, MR findings and imaging follow-up: a study of 53 cases. J Neuroradiol 2001, 28: 230–240.PubMedGoogle Scholar
- Bodi I, Selway R, Bannister P, Doey L, Mullatti N, Elwes R, Honavar M: Diffuse form of Dysembryoplastic Neuroepithelial Tumour: the histological and immunohistochemical features of a distinct entity showing transition to Dysembryoplastic Neuroepithelial Tumour and Ganglioglioma. Neuropathol Appl Neurobiol 2012, 38: 411–425. 10.1111/j.1365-2990.2011.01225.xView ArticlePubMedGoogle Scholar
- Aronica E, Gorter JA, Redeker S, Ramkema M, Spliet WG, van Rijen PC, Leenstra S, Troost D: Distribution, characterization and clinical significance of microglia in glioneuronal tumours from patients with chronic intractable epilepsy. Neuropathol Appl Neurobiol 2005, 31: 280–291. 10.1111/j.1365-2990.2004.00636.xView ArticlePubMedGoogle Scholar
- Gultekin SH, Dalmau J, Graus Y, Posner JB, Rosenblum MK: Anti-Hu immunolabeling as an index of neuronal differentiation in human brain tumors: a study of 112 central neuroepithelial neoplasms. Am J Surg Pathol 1998, 22: 195–200. 10.1097/00000478-199802000-00007View ArticlePubMedGoogle Scholar
- Dalmau J, Furneaux HM, Cordon-Cardo C, Posner JB: The expression of the Hu (paraneoplastic encephalomyelitis/sensory neuronopathy) antigen in human normal and tumor tissues. Am J Pathol 1992, 141: 881–886.PubMedPubMed CentralGoogle Scholar
- Laeng RH, Scheithauer BW, Altermatt HJ: Anti-neuronal nuclear autoantibodies, types 1 and 2: their utility in the study of tumors of the nervous system. Acta Neuropathol 1998, 96: 329–339. 10.1007/s004010050902View ArticlePubMedGoogle Scholar
- Kuba H, Oichi Y, Ohmori H: Presynaptic activity regulates Na(+) channel distribution at the axon initial segment. Nature 2010, 465: 1075–1078. 10.1038/nature09087View ArticlePubMedGoogle Scholar
- Tassi L, Colombo N, Cossu M, Mai R, Francione S, Lo Russo G, Galli C, Bramerio M, Battaglia G, Garbelli R, et al.: Electroclinical, MRI and neuropathological study of 10 patients with nodular heterotopia, with surgical outcomes. Brain 2005, 128: 321–337.View ArticlePubMedGoogle Scholar
- Meroni A, Galli C, Bramerio M, Tassi L, Colombo N, Cossu M, Lo Russo G, Garbelli R, Spreafico R: Nodular heterotopia: a neuropathological study of 24 patients undergoing surgery for drug-resistant epilepsy. Epilepsia 2009, 50: 116–124. 10.1111/j.1528-1167.2008.01717.xView ArticlePubMedGoogle Scholar
- Blumcke I, Wiestler OD: Gangliogliomas: an intriguing tumor entity associated with focal epilepsies. J Neuropathol Exp Neurol 2002, 61: 575–584.View ArticlePubMedGoogle Scholar
- Hirose T, Scheithauer BW, Lopes MB, Gerber HA, Altermatt HJ, VandenBerg SR: Ganglioglioma: an ultrastructural and immunohistochemical study. Cancer 1997, 79: 989–1003. 10.1002/(SICI)1097-0142(19970301)79:5<989::AID-CNCR16>3.0.CO;2-ZView ArticlePubMedGoogle Scholar
- Becker AJ WO, Figarella-Branger D, Blumcke I: Ganglioglioma and gangliocytoma. In WHO Classification of Tumours of the Central Nervous System. Edited by: Louis DN, Ohgaki H, Wiestler OD, Cavenee WK. Lyon: IARC; 2007:103–105.Google Scholar
- Thom M, Toma A, An S, Martinian L, Hadjivassiliou G, Ratilal B, Dean A, McEvoy A, Sisodiya SM, Brandner S: One hundred and one dysembryoplastic neuroepithelial tumors: an adult epilepsy series with immunohistochemical, molecular genetic, and clinical correlations and a review of the literature. J Neuropathol Exp Neurol 2011, 70: 859–878. 10.1097/NEN.0b013e3182302475View ArticlePubMedGoogle Scholar
- Galloway M: CD34 expression in glioblastoma and giant cell glioblastoma. Clin Neuropathol 2010, 29: 89–93. 10.5414/NPP29089View ArticlePubMedGoogle Scholar
- Schindler G, Capper D, Meyer J, Janzarik W, Omran H, Herold-Mende C, Schmieder K, Wesseling P, Mawrin C, Hasselblatt M, et al.: Analysis of BRAF V600E mutation in 1,320 nervous system tumors reveals high mutation frequencies in pleomorphic xanthoastrocytoma, ganglioglioma and extra-cerebellar pilocytic astrocytoma. Acta Neuropathol 2011, 121: 397–405. 10.1007/s00401-011-0802-6View ArticlePubMedGoogle Scholar
- Daumas-Duport C, Scheithauer BW, Chodkiewicz JP, Laws ER Jr, Vedrenne C: Dysembryoplastic neuroepithelial tumor: a surgically curable tumor of young patients with intractable partial seizures. Report of thirty-nine cases. Neurosurgery 1988, 23: 545–556. 10.1227/00006123-198811000-00002View ArticlePubMedGoogle Scholar
- Daumas-Duport CPT, Hawkins C, Shankar SK: Dysembryoplastic neuroepithelial tumor. In WHO Classification of Tumors of the Central Nervous System. 4th edition. Edited by: DN Louis HO, Wiestler OD, Cavenee WK. Lyon: IARC; 2007:99–102.Google Scholar
- Honavar M, Janota I, Polkey CE: Histological heterogeneity of dysembryoplastic neuroepithelial tumour: identification and differential diagnosis in a series of 74 cases. Histopathology 1999, 34: 342–356. 10.1046/j.1365-2559.1999.00576.xView ArticlePubMedGoogle Scholar
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