Table 1 The sources of the antibodies used, and respective pre-treatment and dilution
From: Two cases of multinodular and vacuolating neuronal tumour
Antibody | Dilution | Pre-treatment | Supplier | Code |
|---|---|---|---|---|
Ankyrin-G | 1:100 | H2(20) | NeuroMab | 75-146 |
APP | 1:7500 | H2(10) | Chemicon | MAB348 |
Calbindin | 1:250 | H2(20) | Sigma | C7354 |
Calretinin | 1:100 | H1(20) | Novocastra | NCL-CALRETININ |
CD3 | 1:50 | H2(30) | Dako | M7254 |
CD34 | 1:150 | H2(20) | Dako | M7165 |
CD45 (LCA) | 1:200 | H1(30) | Dako | M0701 |
CD68 (PGM1) | 1:50 | H2(20) | Dako | M0876 |
Chromogranin | 1:100 | H2(20) | Novocastra | NCL-CHROM-430 |
GFAP | 1:1000 | H1(5) | Dako | Z0334 |
HLA-DR (cr3/43) | 1:300 | H2(20) | Dako | M0775 |
HuC/HuD | 1:160 | H1 (30) | Molecular probes | A-21271 |
IDH1 | 1:50 | H1(20) | Dianova | DIA H09 L |
Ki67 | 1:100 | H2(20) | Dako | M7240 |
Nestin | 1:200 | H2(20) | Santa Cruz biotech | sc-23927 |
Neu N | 1:2000 | H1(20) | Chemicon | MAB377 |
NF (pan-NF) | 1:100 | H2(30) | Zymed | 18-0171Z |
NF200 KD | 1:500 | H1(5) | Dako | M0762 |
p62 | 1:100 | H2(20) | BD Transduction labs | 610833 |
Parvalbumin | 1:500 | H2(20) | Millipore (Chemicon) | MAB1572 |
Synaptophysin | 1:40 | H2(20) | Dako | M0776 |
Synuclein | 1:500 | Offline* | BD Transduction labs | 610787 |
Tau (AT8) | 1:500 | H1(20) | Innogenetics | MN1020 |
TDP43-P | 1:1500 | H1(30) | Cosmo Bio Ltd. | TIP-PTD-P02 |