Skip to main content
Download PDF

BRAF V600E mutation mediates FDG-methionine uptake mismatch in polymorphous low-grade neuroepithelial tumor of the young

Acta Neuropathologica Communications volume 8, Article number: 139 (2020) Cite this article

Abstract

We present a case of a 14-year old boy with tumor-associated refractory epilepsy. Positron emission tomography imaging demonstrated a region with heterogeneous high 11C-methionine uptake and a region with homogenous low 18F-fluorodeoxyglucose uptake within the tumor. Histopathological and genomic analyses confirmed the tumor as BRAF V600E-mutated polymorphous low-grade neuroepithelial tumor of the young (PLNTY). Within the high-methionine-uptake region, we observed increased protein levels of L-type amino acid transporter 1 (LAT1), a major transporter of methionine; c-Myc; and constituents of the mitogen-activated protein kinase (MAPK) pathway. We also found that LAT1 expression was linked to the BRAF V600E mutation and subsequent activation of MAPK signaling and c-Myc. Pharmacological and genetic inhibition of the MAPK pathway suppressed c-Myc and LAT1 expression in BRAF V600E-mutated PLNTY and glioblastoma cells. The BRAF inhibitor dabrafenib moderately suppressed cell viability in PLNTY. Collectively, our results indicate that BRAF V600E mutation-activated MAPK signaling and downstream c-Myc induces specific metabolic alterations in PLNTY, and may represent an attractive target in the treatment of the disease.

Introduction

Pediatric low-grade neuroepithelial tumors (P-LGNTs) encompass a group of central nervous system neoplasms that includes long-term epilepsy-associated tumors (LEATs), such as ganglioglioma and dysembryoplastic neuroepithelial tumor (DNT). P-LGNTs have different characteristics than their adult counterparts, and are commonly driven by genomic alterations in the Ras/mitogen-activated protein kinase (MAPK) pathway, such as mutations in BRAF and NF-1 [23, 29]. Recent large-scale genomic studies and genome-wide methylation analyses allowed a thorough characterization of P-LGNTs [24], and cIMPACT-NOW (the Consortium to Inform Molecular and Practical Approaches to CNS Tumor Taxonomy) currently classifies P–LGNTs as distinct disease entities [4, 17]. In 2017, Huse et al. described ten cases of polymorphous low-grade neuroepithelial tumor of the young (PLNTY), which were histologically characterized by oligodendroglioma-like cellular components with intense CD34 immunopositivity. According to previous publications, PLNTYs are indolent tumors that generally exhibit a benign clinical course and harbor either a BRAF V600E mutation or FGFR2/FGFR3 fusion [9]. Based on its histological and genomic profiles, cIMPACT-NOW Update 6 recommends PLNTY as a possible future classification for pediatric-type glial/glioneuronal tumors. However, because of their rare etiology, only a few PLNTYs have been described to date [3, 5, 9, 10, 16, 21, 27, 28], and it is unclear how genomic alterations promote the pathogenesis of the disease. Herein, we present a case of PLNTY with unique metabolic imaging features. Using positron emission tomography (PET), we found regions of heterogeneous high 11C-methionine uptake and homogenous low 18F-fluorodeoxyglucose (FDG) uptake within the tumor. Activation of the MAPK pathway, c-Myc, and expression of L-type amino acid transporter 1 (LAT1) were increased in the high-methionine-uptake area compared with the surrounding cortex (low-methionine-uptake). Glycolytic metabolites were expressed only weakly in tumor cells. Pharmacological and genetic inhibition of the MAPK pathway suppressed c-Myc and LAT1 and inhibited tumor cell viability, suggesting that MAPK signaling and downstream c-Myc activates methionine metabolism and inhibition of this pathway induces therapeutic vulnerability in PLNTY.

Materials and methods

Cell viability analysis

AM-38 and normal human astrocytes was purchased from JCRB Cell Bank and ScienCell Research Laboratories, respectively. Tumorsphere lines were cultured in serum-free neural stem cell medium, as previously described [31]. Normal human astrocytes were cultured with astrocyte medium (ScienCell). To assess cell viability, primary cultured cells were dissociated into single cells and seeded into 96-well plates at a density of 3000 cells/well. After 12 h, dabrafenib (Selleck) and trametinib (Selleck) were serially diluted and added to the wells. Cell viability was measured using the CellTiter-Glo (Promega) assay at day 3, and the results were indicated as % viability of the DMSO control.

shRNA cell line generation

To knockdown BRAF, 293T cells were transfected with lentiviral vector packaging plasmid DNA containing 6 μg of Human BRAF shRNA (#1, TRCN0000381693; #2, TRCN0000196844; Sigma Aldrich), 3.5 μg of pHIV-GP, and 3.5 μg of pVSVg-Rev with Lipofectamine™ 3000 (Thermo Fisher Scientific). YMG62 and AM-38 cells were infected with lentivirus in polybrene (8 μg/mL) for 12 h. Two days later, the cells were selected with puromycin (0.6 μg/mL) for 2 days, and used for experiments. GIPZ non-silencing lentiviral shRNA Control (RHS4348, Horizon Discovery) was used as a non-silencing (NS) control.

Immunohistochemistry

Tumor tissue specimens were fixed in 10% neutral buffered formalin and embedded in paraffin. Hematoxylin and eosin staining was performed using standard procedures. For immunohistochemical analysis, 5-µm-thick sections were deparaffinized, treated with 0.5% H2O2 in methanol, rehydrated, and heated for 20 min for antigen retrieval. After blocking with serum, tissue sections were incubated with primary antibodies against CD34 (Novus Biologicals), LAT1 (Cell Signaling Technology), phospho-MEK (Cell Signaling Technology), phospho-ERK (Bethyl Laboratories), and c-Myc (Cell Signaling Technology) at 4 °C overnight. The next day, sections were washed with PBS, incubated with biotinylated secondary antibody for 30 min at room temperature, and then incubated with ABC solution (PK-6101, PK-6102; Vector laboratories) for 30 min at room temperature. Finally, the sections were incubated with DAB (Dako) and counter-stained with hematoxylin.

Western blotting

Cells were lysed in RIPA buffer (Sigma-Aldrich) with a cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail (Roche). Fifty micrograms of protein was separated by 10% SDS-PAGE gel and transferred to polyvinylidene difluoride membranes (Millipore) by electroblotting. After blocking with 1% or 5% nonfat dry milk in TBST (25 mM Tris [pH, 7.4], 137 mM NaCl, 0.5% Tween20), membranes were incubated at 4 °C overnight with primary antibodies. After washing and incubation with horseradish peroxidase-conjugated secondary antibodies (Cell Signaling Technology), blots were washed, and signals were visualized with chemiluminescent HRP substrate (Millipore). Primary antibodies against BRAF (Gene Tex), c-Myc (Cell Signaling Technology), GAPDH (Gene Tex), LAT1 (Cell Signaling Technology), phospho-MEK (Cell Signaling Technology), phospho-ERK (Bethyl Laboratories), and Vinculin (Novus Biologicals) were used for western blotting.

Case presentation

This study was performed in accordance with declaration of Helsinki and was approved by the Institutional Review Board (Yokohama City University [YCU, Yokohama, Japan], IRB numbers: A1711300006 and B190600002). Written informed consent was obtained from the patient and parents. A 14-year old boy presented with chronic medial temporal lobe epilepsy for a year. Magnetic resonance imaging (MRI) indicated hypointensity on T2-weighted images and hyperintensity on T1-weighted images, with a cystic component in the left temporal lobe. Contrast-enhanced MRI showed no significant enhancement in the lesion (Fig. 1a) while computed tomography revealed heavy calcification. FDG-PET showed lower FDG uptake in the tumor, while 11C-methionine-PET demonstrated increased methionine uptake in the same lesion (SUVmax = 3.9, tumor/normal tissue ratio = 2.9; Fig. 1b). Video-electroencephalographic (EEG) monitoring indicated ictal onset in the left temporal lobe with subsequent spread to the contralateral temporal lobe (Fig. 1c). We speculated that this abnormal lesion was a LEAT. Since we considered this tumor to be completely resectable, the patient underwent craniotomy and resection of the neoplasm, including the high-methionine-uptake region (Fig. 1d). To achieve epileptic control, electrocorticography was performed intraoperatively. After removal of the high-methionine-uptake and T2 hyperintense lesions, the surrounding tissue was resected until interictal epileptiform discharge could no longer be detected by electrocorticography. The patient became epilepsy-free after lesion removal, and MRI indicated complete remission 16 months after the surgery.

Fig. 1
figure 1

Characteristics of a patient with PLNTY. a T2-weighted (left), T1-weighted (middle), and contrast-enhanced (right) MR images. b Computed tomography (CT, left), 18F-fluorodeoxyglucose-PET/CT (middle), and 11C-methionine-PET/CT (right) images. c Video electroencephalography indicating ictal onset in the left temporal lobe, with spread to the contralateral temporal lobe. d PET/CT and MRI merged intraoperative navigation image (left) and surgical image (right) showing the high-methionine-uptake region (#1) and surrounding abnormal lesion (#2) on MRI

Full size image

Tissue samples of the high-methionine-uptake region (#1) and surrounding cortex (low methionine uptake, #2) were collected. Hematoxylin and eosin staining indicated diffusely infiltrating growth patterns and presence of oligodendroglia-like cellular components (Fig. 2a). Astrocytic and high-grade features were absent, with a Ki-67 index of less than 2%. Chicken wire-like branching capillaries and microcalcification were also found in region #1. Despite lower cellularity, oligodendroglia-like cells were present in the surrounding tissue. Immunohistochemistry revealed extensive CD34 expression and peripherally associated ramified neural elements in the tumor cells (Fig. 2a). Targeted DNA sequencing identified a BRAF V600E mutation in the tumor, without recurrent mutations in IDH1, IDH2, TERT promoter, FGFR1, H3F3A, or HIST3H1B (Fig. 2b). Chromosome 1p/19q co-deletion was absent (Fig. 2c). The above histological and genetic features fulfilled the diagnostic criteria for PLNTY.

Fig. 2
figure 2

Histopathologic and genomic features of a patient with PLNTY. a Hematoxylin and eosin (H&E) staining (top) and CD34 immunohistochemistry (bottom) in the high-methionine-uptake (#1) and low-methionine-uptake (#2) region within tumor tissue. Bars, 50 μm. b Sanger sequencing for detection of BRAF V600E (arrow, left) and IDH1 R132H (arrow, right) mutations. c Fluorescence in situ hybridization for detection of 1p31/1q25 (left) and 19q13/19p13 (right) chromosomal deletions

Full size image

To assess the mechanisms underlying the methionine-FDG uptake mismatch indicated by PET, we compared the expression of LAT1, glucose transporter 1 (GLUT-1), and hexokinase-2 (HK-2) between tissue regions #1 and #2. Notably, LAT1, which is a major methionine transporter, was more highly expressed in #1 than in #2 (Fig. 3a). In contrast, GLUT-1 and HK-2, which is correlated with FDG uptake, and lactate dehydrogenase A (LDHA) expression were weak in either region (Additional file 1: Fig. S1). LAT1 expression is mediated by c-Myc activation and BRAF V600E mutation activates the MAPK pathway and downstream c-Myc [8, 32, 33]. Therefore, we hypothesized that BRAF V600E mutation promotes LAT1 expression through MAPK signaling and consequent c-Myc activation in PLNTY. Levels of phospho-MEK, phospho-ERK, and c-Myc were higher in tissue region #1 than in #2 (Fig. 3a), suggesting activation of the MAPK pathway and c-Myc within the high-methionine-uptake lesion. To verify whether the BRAF V600E mutation can induce the expression of LAT1, we exposed primary cultured YMG83 PLNTY cells to a BRAF inhibitor (dabrafenib). As expected, the expression of phospho-MEK, phospho-ERK, c-Myc, and LAT1 was suppressed after dabrafenib treatment in YMG83 cells (Fig. 3b). Notably, BRAF inhibitor (dabrafenib)-treated YMG83 cells had lower cell viability compared to normal human astrocytes (Fig. 3c). To confirm the reproducibility of these molecular features, we used patient-derived YMG62 cells (epithelioid glioblastoma with the BRAF V600E mutation), which exhibited high 11C-methionine uptake by PET imaging (Additional file 1: Fig. S2), and AM-38 glioblastoma cells (BRAF V600E mutant). We found that dabrafenib and a MEK inhibitor (trametinib) inhibited the expression of proteins in the MAPK pathway as well as c-Myc and LAT1 (Fig. 3d and 3e). Similarly, BRAF knockdown suppressed the expression of proteins in the MAPK pathway as well as c-Myc and LAT1 (Fig. 3f). Collectively, these findings indicated that activation of the MAPK pathway by the BRAF V600E mutation deregulates c-Myc and promotes LAT1 expression. This oncogenic signaling pathway increases methionine metabolism and tumor maintenance in PLNTY.

Fig. 3
figure 3

Activating the MAPK pathway induces LAT1 expression in a patient with PLNTY. a Immunohistochemistry of indicated proteins in the high-methionine-uptake (#1) and low-methionine-uptake (#2) regions within tumor tissue. Bars, 50 μm. b Western blot analysis of phospho-MEK, phospho-ERK, c-Myc, and LAT1 proteins in YMG83 (PLNTY, left) cells treated with DMSO and 10 μM BRAF inhibitor (BRAFi, dabrafenib) for 12 h. GAPDH, loading control. c Relative cell viability of dabrafenib-treated (left) and trametinib-treated (right) YMG83 cells and immortalized normal human astrocytes (NHA). *P < 0.05, DMSO versus dabrafenib (left) and trametinib (right). d Western blot analysis for indicated proteins in YMG62 (epithelioid glioblastoma, left) and AM-38 (glioblastoma, right) cells treated with DMSO, 10 μM BRAF inhibitor (BRAFi, dabrafenib), and 10 μM MEK inhibitor (MEKi, trametinib) for 24 h. GAPDH, loading control. e Western blot analysis of BRAF, phospho-MEK, phospho-ERK, c-Myc, and LAT1 proteins in YMG62 (left) and AM-38 (right) cells treated with DMSO and dabrafenib at indicated concentrations. Vinculin, loading control. f Western blot analysis for indicated proteins in non-silencing- (NS) and BRAF- (#1 and #2) transduced YMG62 and AM38 cells. GAPDH, loading control

Full size image

Discussion

Thirty cases of PLNTY have been described to date, with the first ten reported by Huse et al. in 2017 [3, 5, 9, 10, 16, 21, 27, 28]; BRAF V600E mutation was seen in 14 of the patients and BRAF fusion in 1 patient. These BRAF alterations were mutually exclusive with other genomic events, including FGFR3-TACC3 fusion, FGFR3 amplification, FGFR2-CTNNA3 fusion, FGFR2-INA fusion, FGFR2- KIAA1598 fusion, FGFR2 rearrangement, and NTRK2 disruption, suggesting that the vast majority of PLNTYs are induced by BRAF mutation or FGFR fusion and subsequent MAPK activation. Therefore, targeting MAPK signaling may become a potential therapeutic strategy in PLNTY. Indeed, BRAF V600E-mutated PLNTY cells were relatively vulnerable to dabrafenib and trametinib in the present study. Thus, targeted molecular therapy for the MAPK pathway may be particularly useful in PLNTY located in surgically unresectable regions. In addition, Koh et al. reported that the BRAF V600E mutation contributes to the intrinsic epileptogenicity in pediatric brain tumors, and that inhibition of BRAF suppressed epileptic seizures [14]. Thus, BRAF/MEK inhibitors could exert anti-epileptic as well as anti-tumor effects in PLNTY.

PET imaging revealed a region with increased methionine uptake and low FDG uptake within tumor tissue in our patient. Consistent with this finding, previous case reports demonstrated increased methionine uptake but only mild FDG uptake in patients with BRAF V600E-mutated PLNTY [5, 16]. Thus, excessive methionine uptake and low FDG uptake may be imaging features specific to PLNTY. A preclinical study has demonstrated that high uptake of 18F-FDG was correlated with increased Glut-1 and HK-2 expression in human cancers [19]. Although the diagnostic accuracy is insufficient, FDG-PET imaging is useful to differentiate high-grade from low-grade gliomas [1]. In the present case, low FDG uptake and weak expression of Glut-1, HK-2, and LDHA were observed in tumor tissue, suggesting low glycolytic activity in PLNTY. On the other hand, due to a high signal-to-noise ratio, 11C-methionine PET imaging is practical for brain tumors [12, 34]. Several PET imaging studies have demonstrated that methionine uptake was higher in high-grade adult gliomas than in lower-grade gliomas [7, 30]. In epileptogenic brain tumors, however, all gangliogliomas and 37–57% of DNT had increased methionine uptake, although these tumors are classified as WHO grade I [20, 22], implying that methionine uptake may be irrespective of tumor grade in LEATs.

Previous studies have reported that methionine uptake was correlated with LAT1 in gliomas [13, 18]. LAT1 plays a major role in the transport of neutral essential amino acids, including methionine, and is driven by several cancer-related genes such as MYC [25]. It has been demonstrated that c-Myc, which is partly mediated by the MAPK pathway, regulates LAT1 expression and MEK inhibitor suppresses LAT1 (SLC7A5) transcription [6, 8], thereby indicating a role of the MAPK pathway and c-Myc in the regulation of LAT1. Since RAS/MAPK pathway-associated genomic alterations are common in LEATs [24] and that the BRAF V600E mutation has been identified in 20–60% and 30% of gangliogliomas and DNTs, respectively [2, 26], there is a possibility that the BRAF V600E mutation and MAPK pathway-related genomic alterations may activate methionine metabolism in LEATs. To investigate this hypothesis, we evaluated the protein expression of LAT1 and the molecules that are involved in the MAPK pathway. As expected, levels of phospho-MEK, phospho-ERK, c-Myc, and LAT1 were higher in the high-methionine-uptake area than in the low-methionine-uptake area. We also found that genetic and/or pharmacological BRAF inhibition suppressed MAPK pathway activation and attenuated LAT1 expression in BRAF V600E-mutated-PLNTY cells and -glioblastoma cell lines. These findings support the hypothesis that the BRAF V600E mutation may upregulate LAT1 and methionine metabolism through c-Myc activation for cell survival. In addition to LAT1, methionine uptake was correlated with microvascular density (MVD) in gliomas [15]. PLNTYs are considered benign brain neoplasms (proposed as WHO grade I); however, in the present case, a chicken wire-like MVD, which is one of the histopathological characteristics of oligodendroglioma, was also observed in the high-methionine-uptake tissue region. Intriguingly, methionine uptake has been reported to be relatively higher in oligodendrogliomas than in astrocytomas [11]. Thus, PLNTY, which has an oligodendroglioma-like microvascular structure, might show unique metabolic imaging features. Further studies are warranted to validate this hypothesis. Nonetheless, our data indicated that the BRAF V600E mutation induced MAPK pathway activation and downstream c-Myc promoted LAT1 expression and methionine metabolism with little effect on glycolytic pathway activation. These findings may explain the unique metabolic imaging features of FDG-methionine mismatch in PLNTY.

References

  1. Borbely K, Nyary I, Toth M, Ericson K, Gulyas B (2006) Optimization of semi-quantification in metabolic PET studies with 18F-fluorodeoxyglucose and 11C-methionine in the determination of malignancy of gliomas. J Neurol Sci 246:85–94. https://doi.org/10.1016/j.jns.2006.02.015

    Article  CAS  PubMed  Google Scholar 

  2. Chappe C, Padovani L, Scavarda D, Forest F, Nanni-Metellus I, Loundou A, Mercurio S, Fina F, Lena G, Colin C et al (2013) Dysembryoplastic neuroepithelial tumors share with pleomorphic xanthoastrocytomas and gangliogliomas BRAF(V600E) mutation and expression. Brain Pathol 23:574–583. https://doi.org/10.1111/bpa.12048

    Article  PubMed  Google Scholar 

  3. Chen Y, Tian T, Guo X, Zhang F, Fan M, Jin H, Liu D (2020) Polymorphous low-grade neuroepithelial tumor of the young: case report and review focus on the radiological features and genetic alterations. BMC Neurol 20:123. https://doi.org/10.1186/s12883-020-01679-3

    Article  PubMed  PubMed Central  Google Scholar 

  4. Ellison DW, Hawkins C, Jones DTW, Onar-Thomas A, Pfister SM, Reifenberger G, Louis DN (2019) cIMPACT-NOW update 4: diffuse gliomas characterized by MYB, MYBL1, or FGFR1 alterations or BRAF(V600E) mutation. Acta Neuropathol 137:683–687. https://doi.org/10.1007/s00401-019-01987-0

    Article  CAS  PubMed  Google Scholar 

  5. Gupta VR, Giller C, Kolhe R, Forseen SE, Sharma S (2019) Polymorphous low-grade neuroepithelial tumor of the young: a case report with genomic findings. World Neurosurg 132:347–355. https://doi.org/10.1016/j.wneu.2019.08.221

    Article  PubMed  Google Scholar 

  6. Hafliger P, Graff J, Rubin M, Stooss A, Dettmer MS, Altmann KH, Gertsch J, Charles RP (2018) The LAT1 inhibitor JPH203 reduces growth of thyroid carcinoma in a fully immunocompetent mouse model. J Exp Clin Cancer Res 37:234. https://doi.org/10.1186/s13046-018-0907-z

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Hatakeyama T, Kawai N, Nishiyama Y, Yamamoto Y, Sasakawa Y, Ichikawa T, Tamiya T (2008) 11C-methionine (MET) and 18F-fluorothymidine (FLT) PET in patients with newly diagnosed glioma. Eur J Nucl Med Mol Imaging 35:2009–2017. https://doi.org/10.1007/s00259-008-0847-5

    Article  CAS  PubMed  Google Scholar 

  8. Hayashi K, Jutabha P, Endou H, Anzai N (2012) c-Myc is crucial for the expression of LAT1 in MIA Paca-2 human pancreatic cancer cells. Oncol Rep 28:862–866. https://doi.org/10.3892/or.2012.1878

    Article  CAS  PubMed  Google Scholar 

  9. Huse JT, Snuderl M, Jones DT, Brathwaite CD, Altman N, Lavi E, Saffery R, Sexton-Oates A, Blumcke I, Capper D et al (2017) Polymorphous low-grade neuroepithelial tumor of the young (PLNTY): an epileptogenic neoplasm with oligodendroglioma-like components, aberrant CD34 expression, and genetic alterations involving the MAP kinase pathway. Acta Neuropathol 133:417–429. https://doi.org/10.1007/s00401-016-1639-9

    Article  CAS  PubMed  Google Scholar 

  10. Johnson DR, Giannini C, Jenkins RB, Kim DK, Kaufmann TJ (2019) Plenty of calcification: imaging characterization of polymorphous low-grade neuroepithelial tumor of the young. Neuroradiology 61:1327–1332. https://doi.org/10.1007/s00234-019-02269-y

    Article  PubMed  Google Scholar 

  11. Kato T, Shinoda J, Oka N, Miwa K, Nakayama N, Yano H, Maruyama T, Muragaki Y, Iwama T (2008) Analysis of 11C-methionine uptake in low-grade gliomas and correlation with proliferative activity. AJNR Am J Neuroradiol 29:1867–1871. https://doi.org/10.3174/ajnr.A1242

    Article  CAS  PubMed  Google Scholar 

  12. Katsanos AH, Alexiou GA, Fotopoulos AD, Jabbour P, Kyritsis AP, Sioka C (2019) Performance of 18F-FDG, 11C-methionine, and 18F-FET PET for glioma grading: a meta-analysis. Clin Nucl Med 44:864–869. https://doi.org/10.1097/RLU.0000000000002654

    Article  PubMed  Google Scholar 

  13. Kobayashi K, Ohnishi A, Promsuk J, Shimizu S, Kanai Y, Shiokawa Y, Nagane M (2008) Enhanced tumor growth elicited by L-type amino acid transporter 1 in human malignant glioma cells. Neurosurgery 62:493–503; discussion 503–494 https://doi.org/10.1227/01.neu.0000316018.51292.19

  14. Koh HY, Kim SH, Jang J, Kim H, Han S, Lim JS, Son G, Choi J, Park BO, Heo WD et al (2018) BRAF somatic mutation contributes to intrinsic epileptogenicity in pediatric brain tumors. Nat Med 24:1662–1668. https://doi.org/10.1038/s41591-018-0172-x

    Article  CAS  PubMed  Google Scholar 

  15. Kracht LW, Friese M, Herholz K, Schroeder R, Bauer B, Jacobs A, Heiss WD (2003) Methyl-[11C]- l-methionine uptake as measured by positron emission tomography correlates to microvessel density in patients with glioma. Eur J Nucl Med Mol Imaging 30:868–873. https://doi.org/10.1007/s00259-003-1148-7

    Article  CAS  PubMed  Google Scholar 

  16. Lelotte J, Duprez T, Raftopoulos C, Michotte A (2020) Polymorphous low-grade neuroepithelial tumor of the young: case report of a newly described histopathological entity. Acta Neurol Belg 120:729–732. https://doi.org/10.1007/s13760-019-01241-0

    Article  CAS  PubMed  Google Scholar 

  17. Louis DN, Wesseling P, Aldape K, Brat DJ, Capper D, Cree IA, Eberhart C, Figarella-Branger D, Fouladi M, Fuller GNet al (2020) cIMPACT-NOW update 6: new entity and diagnostic principle recommendations of the cIMPACT-Utrecht meeting on future CNS tumor classification and grading. Brain Pathol. https://doi.org/10.1111/bpa.12832

  18. Okubo S, Zhen HN, Kawai N, Nishiyama Y, Haba R, Tamiya T (2010) Correlation of L-methyl-11C-methionine (MET) uptake with L-type amino acid transporter 1 in human gliomas. J Neurooncol 99:217–225. https://doi.org/10.1007/s11060-010-0117-9

    Article  CAS  PubMed  Google Scholar 

  19. Ong LC, Jin Y, Song IC, Yu S, Zhang K, Chow PK (2008) 2-[18F]-2-deoxy-d-glucose (FDG) uptake in human tumor cells is related to the expression of GLUT-1 and hexokinase II. Acta Radiol 49:1145–1153. https://doi.org/10.1080/02841850802482486

    Article  PubMed  Google Scholar 

  20. Rheims S, Rubi S, Bouvard S, Bernard E, Streichenberger N, Guenot M, Le Bars D, Hammers A, Ryvlin P (2014) Accuracy of distinguishing between dysembryoplastic neuroepithelial tumors and other epileptogenic brain neoplasms with [(1)(1)C]methionine PET. Neuro Oncol 16:1417–1426. https://doi.org/10.1093/neuonc/nou022

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Riva G, Cima L, Villanova M, Ghimenton C, Sina S, Riccioni L, Munari G, Fassan M, Giangaspero F, Eccher A (2018) Low-grade neuroepithelial tumor: unusual presentation in an adult without history of seizures. Neuropathology 38:557–560. https://doi.org/10.1111/neup.12504

    Article  CAS  PubMed  Google Scholar 

  22. Rosenberg DS, Demarquay G, Jouvet A, Le Bars D, Streichenberger N, Sindou M, Kopp N, Mauguiere F, Ryvlin P (2005) [11C]-Methionine PET: dysembryoplastic neuroepithelial tumours compared with other epileptogenic brain neoplasms. J Neurol Neurosurg Psychiatry 76:1686–1692. https://doi.org/10.1136/jnnp.2004.051607

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Ryall S, Tabori U, Hawkins C (2020) Pediatric low-grade glioma in the era of molecular diagnostics. Acta Neuropathol Commun 8:30. https://doi.org/10.1186/s40478-020-00902-z

    Article  PubMed  PubMed Central  Google Scholar 

  24. Ryall S, Zapotocky M, Fukuoka K, Nobre L, Guerreiro Stucklin A, Bennett J, Siddaway R, Li C, Pajovic S, Arnoldo A et al (2020) Integrated molecular and clinical analysis of 1,000 pediatric low-grade gliomas. Cancer Cell 37(569–583):e565. https://doi.org/10.1016/j.ccell.2020.03.011

    Article  CAS  Google Scholar 

  25. Salisbury TB, Arthur S (2018) The regulation and function of the l-type amino acid transporter 1 (LAT1) in cancer. Int J Mol Sci. https://doi.org/10.3390/ijms19082373

    Article  PubMed  PubMed Central  Google Scholar 

  26. Schindler G, Capper D, Meyer J, Janzarik W, Omran H, Herold-Mende C, Schmieder K, Wesseling P, Mawrin C, Hasselblatt M et al (2011) Analysis of BRAF V600E mutation in 1,320 nervous system tumors reveals high mutation frequencies in pleomorphic xanthoastrocytoma, ganglioglioma and extra-cerebellar pilocytic astrocytoma. Acta Neuropathol 121:397–405. https://doi.org/10.1007/s00401-011-0802-6

    Article  CAS  Google Scholar 

  27. Sumdani H, Shahbuddin Z, Harper G, Hamilton L (2019) Case report of rarely described polymorphous low-grade neuroepithelial tumor of the young and comparison with oligodendroglioma. World Neurosurg 127:47–51. https://doi.org/10.1016/j.wneu.2019.03.181

    Article  PubMed  Google Scholar 

  28. Surrey LF, Jain P, Zhang B, Straka J, Zhao X, Harding BN, Resnick AC, Storm PB, Buccoliero AM, Genitori L et al (2019) Genomic analysis of dysembryoplastic neuroepithelial tumor spectrum reveals a diversity of molecular alterations dysregulating the MAPK and PI3K/mTOR pathways. J Neuropathol Exp Neurol 78:1100–1111. https://doi.org/10.1093/jnen/nlz101

    Article  PubMed  Google Scholar 

  29. Tateishi K, Nakamura T, Yamamoto T (2019) Molecular genetics and therapeutic targets of pediatric low-grade gliomas. Brain Tumor Pathol 36:74–83. https://doi.org/10.1007/s10014-019-00340-3

    Article  PubMed  Google Scholar 

  30. Tateishi K, Tateishi U, Nakanowatari S, Ohtake M, Minamimoto R, Suenaga J, Murata H, Kubota K, Inoue T, Kawahara N (2014) (62)Cu-diacetyl-bis (N(4)-methylthiosemicarbazone) PET in human gliomas: comparative study with [(18)F]fluorodeoxyglucose and L-methyl-[(11)C]methionine PET. AJNR Am J Neuroradiol 35:278–284. https://doi.org/10.3174/ajnr.A3679

    Article  CAS  PubMed  Google Scholar 

  31. Wakimoto H, Kesari S, Farrell CJ, Curry WT Jr, Zaupa C, Aghi M, Kuroda T, Stemmer-Rachamimov A, Shah K, Liu TC et al (2009) Human glioblastoma-derived cancer stem cells: establishment of invasive glioma models and treatment with oncolytic herpes simplex virus vectors. Cancer Res 69:3472–3481. https://doi.org/10.1158/0008-5472.CAN-08-3886

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. Yue M, Jiang J, Gao P, Liu H, Qing G (2017) Oncogenic MYC activates a feedforward regulatory loop promoting essential amino acid metabolism and tumorigenesis. Cell Rep 21:3819–3832. https://doi.org/10.1016/j.celrep.2017.12.002

    Article  CAS  PubMed  Google Scholar 

  33. Zhang W, Liu HT (2002) MAPK signal pathways in the regulation of cell proliferation in mammalian cells. Cell Res 12:9–18. https://doi.org/10.1038/sj.cr.7290105

    Article  CAS  PubMed  Google Scholar 

  34. Zhao C, Zhang Y, Wang J (2014) A meta-analysis on the diagnostic performance of (18)F-FDG and (11)C-methionine PET for differentiating brain tumors. AJNR Am J Neuroradiol 35:1058–1065. https://doi.org/10.3174/ajnr.A3718

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

We thank Mrs. Emi Hirata and Yasuko Tanaka (YCU) for technical and administrative assistance. We also would like to thank Editage (www.editage.com) for English language editing.

Funding

This work was supported by Grant-Aid for Scientific Research (19K09488), Princess Takamatsu Cancer Research Fund, Takeda Science Foundation, SGH Cancer foundation, Yokohama Foundation for Advancement of Medical Science, and Bristol-Myers Squibb Foundation.

Author information

Authors and Affiliations

  1. Department of Neurosurgery, Graduate School of Medicine, Yokohama City University, 3-9 Fukuura, Kanazawa, Yokohama, 2360004, Japan

    Kensuke Tateishi, Naoki Ikegaya, Jo Sasame, Takahiro Hayashi, Yohei Miyake, Hidetoshi Murata & Tetsuya Yamamoto

  2. Department of Pathology, Yokohama City University Hospital, Yokohama, Japan

    Naoko Udaka & Shoji Yamanaka

  3. Department of Radiology, Graduate School of Medicine, Yokohama City University, Yokohama, Japan

    Tetsuhiko Okabe & Daisuke Utsunomiya

  4. Departmento of Radiology, Division of Nuclear Medicine, National Center for Global Health and Medicine, Tokyo, Japan

    Ryogo Minamimoto

Authors
  1. Kensuke Tateishi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Naoki Ikegaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Naoko Udaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jo Sasame
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Takahiro Hayashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yohei Miyake
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Tetsuhiko Okabe
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Ryogo Minamimoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Hidetoshi Murata
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Daisuke Utsunomiya
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Shoji Yamanaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Tetsuya Yamamoto
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

KT led the study, collected samples, designed experiments, performed experiments, interpreted data, and wrote the manuscript. JS, TH, and YM performed experiments. NI, HM provided tumor samples and associated clinical details. TO, RM, and DU interrupted PET and MRI studies. NU, and SY performed the histological classification of tumor samples. TY designed experiments and interpreted data. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Kensuke Tateishi.

Ethics declarations

Competing interests

The authors declare that they have no competing interests.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary information

Additional file 1: Figure S1.

Low glycolysis activation in a patient with PLNTY. Immunohistochemistry for glucose transporter 1, hexokinase 2, and lactate dehydrogenase A in the high-methionine-uptake (#1, upper) and low-methionine-uptake (#2, lower) region within tumor tissue. A. Bars, 50 μm. Figure S2. Images of the patient’s glioblastoma with the BRAF V600E mutation. Contrast-enhanced magnetic resonance (left) and 11C-methionine positron emission tomography (right) images of the YMG62 patient.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Tateishi, K., Ikegaya, N., Udaka, N. et al. BRAF V600E mutation mediates FDG-methionine uptake mismatch in polymorphous low-grade neuroepithelial tumor of the young. acta neuropathol commun 8, 139 (2020). https://doi.org/10.1186/s40478-020-01023-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1186/s40478-020-01023-3

Keywords

  • PLNTY
  • BRAF V600E mutation
  • Methionine PET
  • LAT1

Acta Neuropathologica Communications

ISSN: 2051-5960

Contact us