Fig. 8
Multiple ApoER2-Dab1 components accumulate in ApoER2-expressing neurons and NPs in the ProS-CA1 border region in early MCI. Serial coronal sections of the ProS-CA1 border region in a representative early MCI case (Braak stage III, MMSE 28/30) were probed with antibodies targeting ApoER2-Dab1 pathway components. Single-target IHC revealed regional co-accumulation of multiple ApoER2-Dab1 pathway markers including pTauSer202/Thr205 (A1–5), Dab1 (A6–7), pDab1Y220 (A8–10), pP85αTyr607 (A11–15), pLIMK1Thr508 (A16–20), and pPSD95Thr19 (A20–55). Affected neurons, GVD-like structures, and the apparent locations of extracellular plaques are designated with solid arrows, open arrows, and stars, respectively. Neighboring neurons with no evident accumulations of ApoER2-Dab1 components are designated with an * in A10–25. MP-IHC suggested that pTauSer202/Thr205, Dab1, pP85αTyr607, and pPSD95Thr19 accumulate together within a subset of ApoER2-expressing basal pyramidal neurons (open arrows in B1–5), and within MAP2- and ApoER2-immunoreactive globular (yellow arrows in B1–10) or thread-like (white arrows in B1–5) dystrophic dendrites in the vicinity of affected neurons and ApoE-enriched NPs (white star in B6–10). NFL is not included in merged images (B5, B10) to enhance visualization. Scale bars in MP-IHC images = 10 µm