Fig. 8

Scanning electron microscopy (SEM) images of glass spheres incubated with PMSA substrate. In order to confirm the adsorption of rec-PrP from PMSA substrates onto the surface of glass beads, glass spheres with a diameter of 9 to 13 µm were incubated with PMSA substrate containing bank vole rec-PrP for 2 h. The spheres were then thoroughly washed with PBS and coated with gold for visualization using SEM. Control experiments included glass spheres incubated in PBS (clean glass sphere) and in PMSA substrate devoid of rec-PrP (glass sphere with CB + sulphated dextran), which underwent the same treatment and visualization process. The SEM micrographs clearly show a smooth coating on the spheres incubated with complete PMSA substrate, likely corresponding to rec-PrP, while the spheres incubated in solutions without rec-PrP display a distinct appearance, confirming that rec-PrP is being adsorbed to glass surfaces