Common molecular features of H3K27M DMGs and PFA ependymomas map to hindbrain developmental pathways
Acta Neuropathologica Communications volume 11, Article number: 25 (2023)
Globally decreased histone 3, lysine 27 tri-methylation (H3K27me3) is a hallmark of H3K27-altered diffuse midline gliomas (DMGs) and group-A posterior fossa ependymomas (PFAs). H3K27-altered DMGs are largely characterized by lysine-to-methionine mutations in histone 3 at position 27 (H3K27M). Most PFAs overexpress EZH inhibitory protein (EZHIP), which possesses a region of similarity to the mutant H3K27M. Both H3K27M and EZHIP inhibit the function of the polycomb repressive complex 2 (PRC2) responsible for H3K27me3 deposition. These tumors often arise in neighboring regions of the brainstem and posterior fossa. In rare cases PFAs harbor H3K27M mutations, and DMGs overexpress EZHIP. These findings together raise the possibility that certain cell populations in the developing hindbrain/posterior fossa are especially sensitive to modulation of H3K27me3 states. We identified shared molecular features by comparing genomic, bulk transcriptomic, chromatin-based profiles, and single-cell RNA-sequencing (scRNA-seq) data from the two tumor classes. Our approach demonstrated that 1q gain, a key biomarker in PFAs, is prognostic in H3.1K27M, but not H3.3K27M gliomas. Conversely, Activin A Receptor Type 1 (ACVR1), which is associated with mutations in H3.1K27M gliomas, is overexpressed in a subset of PFAs with poor outcome. Despite diffuse H3K27me3 reduction, previous work shows that both tumors maintain genomic H3K27me3 deposition at select sites. We demonstrate heterogeneity in shared patterns of residual H3K27me3 for both tumors that largely segregated with inferred anatomic tumor origins and progenitor populations of tumor cells. In contrast, analysis of genes linked to H3K27 acetylation (H3K27ac)-marked enhancers showed higher expression in astrocytic-like tumor cells. Finally, common H3K27me3-marked genes mapped closely to expression patterns in the human developing hindbrain. Overall, our data demonstrate developmentally relevant molecular similarities between PFAs and H3K27M DMGs and support the overall hypothesis that deregulated mechanisms of hindbrain development are central to the biology of both tumors.
Advances in high-throughput sequencing have provided incredible windows into understanding the diversity and heterogeneity in cancers. Genomic, transcriptomic, and epigenomic studies have aided in molecular classification of tumors, elucidation of candidate driving events, and identification of potential cells of origin for many cancers, including pediatric brain tumors. Nevertheless, brain tumors remain the leading cause of mortality among all pediatric cancers . Unlike in adult populations, pediatric brain tumors frequently arise in infratentorial regions , suggesting a spatiotemporal susceptibility to tumorigenesis. Indeed, transcriptional analysis of pediatric brain tumors including medulloblastoma, gliomas, and ependymomas suggest shared expression programs with certain developmental lineages [3,4,5,6,7,8,9,10].
Group A posterior fossa ependymomas (PFAs) and diffuse midline gliomas (DMGs), H3K27-altered, are characterized by global reduction of the repressive histone modification histone 3 lysine 27 trimethylation (H3K27me3) [11,12,13,14,15]. Global H3K27me3 depletion is mediated by inhibition of the function of the polycomb repressive complex-2 (PRC2), which contains the H3K27-specific methyltransferase enhancer of zeste homolog 2 (EZH2). The majority of these DMGs harbor missense mutations in histone 3-encoding genes (primarily H3-3A and H3C2) [13,14,15] that result in a lysine-to-methionine substitution at position 27 (H3K27M). This histone tail mutation inhibits PRC2 activity [11, 12, 16]. Most PFAs, in contrast, exhibit overexpression of EZH inhibitory protein (EZHIP) [17, 18] that similarly suppresses PRC2 activity. EZHIP contains a methionine residue at position 406 that mimics the H3K27M mutant histone [19,20,21,22,23]. In addition to depleting H3K27me3, each of these tumors demonstrates increased global levels of the activation-associated mark H3K27 acetylation (H3K27ac) [18, 20, 22,23,24,25,26,27].
With their epigenetic similarities, H3K27M DMGs and PFAs also share sensitivities to inhibitors targeting histone-modifying enzymes, including histone lysine demethylases, and histone deacetylases [25,26,27,28,29,30]. Although H3K27me3 levels are greatly reduced globally, specific regions of the genome retain H3K27me3, most of which are CpG islands commonly known to be canonical PRC2 binding sites [11, 20, 31,32,33]. Moreover, both tumor types appear to rely on residual EZH2 activity. Both pharmacologic and genetic inhibition of EZH2 reduce tumor viability in models of H3K27M DMGs and PFAs [33,34,35], suggesting that the tumors rely on residual PRC2 activity.
In addition to their molecular similarities, PFAs and H3K27M DMGs arise in nearby structures associated with the hindbrain. The floor of the fourth ventricle is formed by the pons and the roof by the cerebellum. Whereas the majority of H3K27M DMGs arise from the pons, most PFAs are found in association with the fourth ventricle and cerebellum [36, 37]. Moreover, it is increasingly recognized that a rare population of H3-wildtype, low-H3K27me3 DMGs overexpress EZHIP, and that a similarly small percentage of PFAs harbor H3K27M mutations [18, 38,39,40]. The extent to which these two tumor subtypes share biology, and how this relates to hindbrain development, remains an unanswered question. To address this gap in our knowledge, we hypothesized that shared molecular features of H3K27M DMGs and PFAs will reveal key programs within the developing human hindbrain. We addressed this hypothesis by examining genomic, transcriptomic, and epigenomic characterizations of each tumor type to characterize shared biology to better understand the origins and potential vulnerabilities of these tumors.
Copy number alteration analyses
Arm-length copy number alterations for PFAs and DMGs were obtained from supplementary data of published datasets [36, 37]. For tumor samples from the NCI, tumors were classified with the Molecular Neuropathology classifier (v11b6) . Genomic Identification of Significant Targets in Cancer (GISTIC) version 2.0.23 was used to identify and score broad copy number events. Score cutoffs of ± 0.3 were used to call a gain or loss of a chromosome arm consistent with the methods employed by Mackay et al. .
Gene expression analyses
PFA (GSE100240, GSE64415) microarray expression data and DMG (Mackay et al. ) meta-analysis expression data were obtained. A limited differential expression analysis was conducted by splitting the expression datasets by the variable of interest and applying a two-sided student’s t test. Multiple testing correction was applied by the Benjamini–Hochberg method. For gene set enrichment analysis (GSEA), ranked gene lists were generated by taking the product of the negative log-10 transformation of the q-value and the difference in expression for each gene. GSEA 4.2.3 was used to run GSEA Preranked on the resulting gene lists with the following parameters: 15–500 as min–max gene set size; weighted scoring scheme; meandiv normalization; Abs_max_of_probes mode. For standard deviation analyses, the probe with the maximum average value across samples in PFAs was used. Any genes that did not appear in both gene sets were excluded from plotting.
Histone 3 lysine 27 (H3K27) trimethylation ChIP-seq analyses
For samples from Bender et al. , BED files with hg19 alignment were obtained from the authors. BED files aligned to hg19 from Harutyunyan et al.  were obtained from the Genetics and Genomics Analysis Platform (GenAP). Data aligned to hg19 from Bayliss et al.  (GSE89451) and Mack et al.  (GSE89451) were obtained from GEO. Bedtools 2.29.2 was used to intersect peaks from each dataset with a custom BED file containing genes and their promoters defined as 2 kb upstream from the transcription start site (TSS). Genes with peaks in greater than two-thirds of tumor samples for each class of tumor were defined as common H3K27me3-retaining genes and used for downstream analyses.
H3K27ac and enhancer analyses
We obtained published H3K27M DMG-specific enhancer-associated genes from Krug et al.  and PFA-specific enhancer-associated genes from Mack et al. . We computed the overlap in genes from these two gene sets. Each of these curated gene sets was in part derived from comparison against enhancer profiles of other brain tumors. To identify enhancers that were unique to each tumor type independent of these comparisons with non-PFAs and non-H3K27M DMGs, we accessed the ChIP-seq files available from each study and reanalyzed them. We conducted Rank-ordering of Super Enhancer (ROSE) analysis to call super enhancers for each tumor. We examined if PFA-specific enhancers from the original analysis were called as super enhancers in any of the DMG ChIP-seq samples. If an enhancer appeared at least once in the DMG samples, we included it the shared enhancer list. We repeated the converse process for DMG-specific enhancers. This method allowed us to avoid biases generated by comparisons with non-PFAs and non-H3K27M DMGs. Pathway analyses of shared or unique enhancers were completed using Enrichr [43,44,45]. Volcano plots were generated using code generated by Appyter.
Single-cell RNA-seq analyses
Published RNA-Seq by Expectation–Maximization (RSEM) values were obtained for PFA and DMG datasets [4, 6] and loaded as Seurat objects using Seurat (4.1.1) and SeuratObject (4.1.0). Each dataset was independently normalized, and then variable features were selected. Seurat’s SelectIntegrationFeatures and FindIntegrationAnchors were sequentially applied to the datasets, and then IntegrateData was applied based on the anchors identified. The resulting integrated dataset underwent dimensional reduction and clustering. FindConservedMarkers was used to identify differentially expressed genes by cluster. Developing brain single-cell RNA-seq data from Aldinger et al.  were obtained from https://cbl-dev.cells.ucsc.edu and analyzed using Seurat. AddModuleScore was used to generate a score for the H3K27me3-retained gene signature. Single-cell RNA-seq data from Eze et al.  was obtained from https://cells-test.gi.ucsc.edu/?ds=early-brain and processed using Seurat as described in , and CRABP1 feature plots were generated using the Seurat ViolinPlot function, ggplot2 (3.3.5), and ggprism (1.0.3). For area under the curve measurements from the Sepp et al.  dataset, raw count measurements were processed with AUCell (from Bioconductor 3.16) using the H3K27me3-retained gene signature.
Allen Brain Atlas analyses
Human data were obtained from BrainSpan, and mouse data were obtained from the Allen Developing Mouse Brain map. All resulting analyses were completed with GraphPad Prism 9.1.1.
Graphs were plotted and statistical analyses were performed using Prism software (versions 9.1.1, Graphpad, La Jolla, CA). Data are represented as the means ± standard deviation (S.D.) or as violin plots. The sample size (n) along with the statistical test performed and corresponding p-values are indicated in each figure or figure legend. Progression-free and overall survival metadata, when available, was analyzed comparing survival curves utilized the Log-Rank (Mantel-Cox) test for significance. Unpaired two-tailed, two-sided, Student’s t test or one-way ANOVA followed by multiple comparisons analysis using either Ordinary one-way ANOVA or Kruskal–Wallis tests were used to analyze data. Data were considered significant if p-values, adjusted where appropriate, were below 0.05 (95% confidence intervals).
H3.1K27M and EZHIP-DMGs share key copy-number alterations with PFAs
To characterize the shared features of H3K27M DMG and PFAs, we began by comparing copy number changes in two published data sets and from Mackay et al. (, H3K27M DMG = 295) and Pajtler et al. (, PFA = 240) [18, 36]. Overall, H3K27M DMGs demonstrated far greater genomic instability compared to PFAs. Nevertheless, comparison of copy number alterations demonstrated that PFAs harbor several recurrent chromosome arm alterations that are shared with H3K27M DMGs. These included gain of the long arm of chromosome 1 (1q) and loss of 6q (Fig. 1a). We validated these findings in two independent, non-overlapping tumor cohorts (PFA = 573 and DMG = 271) curated by the National Cancer Institute (NCI) (Fig. 1b).
Gain of 1q has previously been associated with worse overall survival outcome in PFAs in multiple studies [18, 49,50,51,52], a finding reproduced in our investigation of the NCI PFA cohort (Additional file 1: Fig. S1a). We noted that 1q gain was also the most common recurrent arm-length gain in H3K27M DMGs; however, there was no significant difference in overall survival in H3K27M DMGs with or without 1q gain from the published cohorts (Additional file 1: Fig. S1b). Previous work had demonstrated differences in the copy-number landscapes based on the type of H3 mutation with H3.3- versus (vs.) H3.1-mutant tumors exhibiting increased genomic instability . We examined copy number trends with respect to H3.3 vs. H3.1 status in the NCI cohort and evaluated how H3 wildtype (H3-WT) EZHIP-expressing DMGs compared to each histone subtype. We observed that H3.1-mutant and EZHIP-expressing DMGs had fewer arm-length copy-number events (Fig. 1c) and focal amplifications and deletions (Fig. 1d) compared to H3.3 mutant DMGs. Gain of 1q was observed at a higher frequency in H3.1 vs. H3.3K27M DMGs in both the Mackay et al. (H3.1 = 35.1% vs. H3.3 = 10.5%; Fig. 1e) and the NCI cohort (H3.1 = 41.7% vs. H3.3 = 23.9%; Fig. 1f). Notably, half of EZHIP-expressing DMGs also exhibited 1q gain (Fig. 1f). Unexpectedly, 1q gain was associated with better overall survival in H3.1K27M tumors (Fig. 1g), but not in H3.3K27M-mutant tumors (Fig. 1h) or in other pediatric brain tumors—including other pediatric gliomas [36, 53], other posterior fossa ependymomas , and medulloblastomas  with the exception of a small sample-size cohort of group 4 medulloblastoma (Additional file 1: Fig. S1c–h). This intriguing finding of a 1q gain survival association in H3.1K27M tumors was unable to be assessed further in the NCI cohort as the majority of these tumors did not have sequencing information to distinguish H3.1 from H3.3 mutations.
In addition to recurrent 1q gain, the other alterations observed in at least 5% of each cohort were losses of 6q, 10q, and 22q (Fig. 1a, b). Loss of 6q has recently been associated with a subset of PFAs with poor survival rates . Assessment of the 6q loss in the DMG cohort demonstrated a trend toward worse survival in 6q loss tumors (Additional file 1: Fig. S1i). In H3K27M DMGs, there was no major difference in 6q status when comparing H3.1 and H3.3-mutant cohorts (Additional file 1: Fig. S1j). Given the genomic instability of DMGs in general, we asked if 6q alterations were associated with high arm-level alterations. In every cohort, tumors with 6q loss were associated with higher levels of arm-level losses across the genome. In contrast, the frequency of arm gains in these tumors was not significantly different from non-6q loss tumors in each cohort (Additional file 1: Fig. S1k). We hypothesized that despite the relatively quiescent genome PFAs exhibit compared to H3K27M DMGs, the two tumor types might share certain alterations that permit or enhance tumorigenicity in the context of H3K27me3 dysregulation. Our findings highlight a spectrum of genomic alteration patterns that varies by driving alteration with H3.3-mutant gliomas exhibiting the greatest alteration frequencies.
ACVR1 expression is prognostic in PFAs and is associated with transcriptomic signatures observed in ACVR1-mutant DMGs
Due to our observed differences between H3.1 and H3.3 K27M DMGs and 1q status, we extended our comparative analysis to include other genomic alterations that segregate with mutant histone subtypes. Activating mutations in activin A receptor type 1 (ACVR1) occur more frequently in H3.1 vs. H3.3 DMGs [36, 57,58,59]. Concordant with our findings of 1q gain enrichment in H3.1 tumors, we observed increased frequency of ACVR1 mutations in 1q gain tumors vs. non-1q gain tumors (Fig. 2a).
Recent work has identified rare mutations of ACVR1 in PFA tumors . These findings prompted us to investigate ACVR1 in PF ependymomas. First, we observed increased expression of ACVR1 in PFAs compared to group B posterior fossa ependymomas (PFBs), a subtype of PF ependymomas that lacks EZHIP expression  (Fig. 2b). We found tumors with the highest levels of ACVR1 expression in the Pajtler et al. cohort to be associated with worse progression-free and overall survival outcomes (Fig. 2c, d). We next investigated whether ACVR1-mutant H3K27M DMGs and ACVR1-high PFAs shared transcriptomic signatures. We found that both tumors demonstrated enrichment of many ACVR1-mutant  and H3.1K27M-enriched  pathways including hypoxia/HIF1-α transcription factor network and epithelial-to-mesenchymal transition, as well as glycolysis, TNFα/NFκB signaling, and mTORC1 signaling (Fig. 2e–g).
H3K27me3 commonly enriched genes in PFAs and H3K27M DMGs exhibit heterogeneity corresponding to tumor anatomic location
We next focused our efforts on defining common epigenetic signatures in H3K27M DMGs and PFAs. Despite global reduction of H3K27me3, multiple studies have demonstrated that both tumors retain H3K27me3 at specific genomic sites [11, 16, 21, 32, 33, 35]. Prior studies have established that the patterns of retained H3K27me3 are distinct from other pediatric brain tumors [11, 33] and have pointed to several shared sites. While specific loci such as the CDKN2A locus have been pointed out as key sites of retained H3K27me3 in data from both tumor subtypes, more global analyses of the similarities and differences in H3K27me3 landscapes in these tumors are lacking. We performed a systematic comparison of the genes that retain H3K27me3 in H3K27M DMGs and PFAs to better understand this shared chromatin landscape.
H3K27me3 chromatin-immunoprecipitation with high throughput sequencing (ChIP-seq) profiles generated by our group  and others [11, 32, 33] were analyzed. We examined H3K27me3 enrichment in nine PFA ependymomas and six DMGs to define genes with commonly methylated promoters and gene bodies between the two tumors. We identified 551 genes with H3K27me3 retained in both tumor sets (Fig. 3a). Because H3K27me3 is a repressive mark, the extent of repression was examined by analyzing standard deviation values of gene expression for each of these 551 genes within both H3K27M DMGs and PFAs. Analysis of bulk expression data from both tumor cohorts identified high variance in expression levels of several H3K27me3-associated genes in both PFAs and H3K27M DMGs with greatest variability in homeobox genes including HOXA2, HOXA4, and LHX2. (Fig. 3b). Examination of genomic H3K27me3 at HOX gene clusters in PFAs, H3K27M DMGs, and patient-derived H3K27M-DMG cell lines demonstrated distinct variations in enrichment (Fig. 3c). In both tumor sets, some samples showed H3K27me3 enrichment along the entire length of the HOXA and HOXD clusters, but others demonstrated variability in H3K27me3 at the HOXA1 through HOXA7 loci and the proximal HOXD locus (Fig. 3c).
HOXA2, HOXA3, and HOXA4 have been identified as transcriptional markers that differentiate PFA1 and PFA2 . Pajtler et al. proposed that the PFA1 tumors overexpress HOXA-family genes and hypothesized distinct anatomical origins PFA1 vs. PFA2 ependymomas . Assessment of HOXA2 bulk expression revealed a bimodal expression pattern in PFAs (Fig. 3d). As expected, segregation of HOXA2 high vs. low PFAs occurred almost completely along with PFA1 vs. PFA2 status with majority of HOXA2 low tumors corresponding to PFA2 subgroup (Fig. 3e). H3K27M DMGs similarly exhibited bimodal HOXA2-high vs. low distribution (Fig. 3f). Moreover, HOXA2 expression levels revealed similar anatomic segregation with majority of thalamic tumors demonstrating low HOXA2 expression (Fig. 3g). HOXA4 expression demonstrated a similar, though less striking pattern (Additional file 1: Fig. S2a–d). HOXD3, for which expression data in PFAs was unavailable, also followed a similar bimodal distribution (Additional file 1: Fig. S2e) and segregation with pontine tumors in HOXD3-high H3K27M DMGs (Additional file 1: Fig. S2f). Evidence of HOX family expression in single-cell data from DMGs and PFAs [4, 6] further supported this anatomic pattern in expression. Tumors derived from the pons and PFA1 showed higher HOXA2 and HOXA4 expression, while HOXD3 expression was elevated in pontine vs. thalamic tumors but was not significantly different between PFA1 and PFA2 tumors (Figs. 3h and Additional file 1: Fig. S2g). This distinction in HOX gene expression patterns between DMGs and PFAs may reflect subtle regional differences in the cells of origin; however, HOXD3 expression data from additional PFA tumors will be necessary to validate this hypothesis.
Differential H3K27me3 enrichment at these loci could be due to varying effects of PRC2 inhibition by the H3K27M mutation. To test this hypothesis, we examined published ChIP-seq tracks from DIPG-XIII and BT245 H3K27M patient-derived cell lines with or without H3K27M knockdown . In both cell lines, H3K27M knockdown did not alter H3K27me3 genomic distribution at these genomic loci (Fig. 3i), suggesting that differential H3K27me3 at this site is not dependent on H3K27M. Overall, both H3K27M DMGs and PFA ependymomas showed differences in H3K27me3 genomic distribution at specific gene loci consistent with gene expression differences based on tumor anatomic location. These patterns are in contrast with the initial hypothesis that H3K27M and EZHIP might drive expression patterns through modulating the degree of retained H3K27me3 at PRC2-target sites and highlight the importance of the anatomic sites of origin on this epigenetic signature.
Enhancer signatures in PFAs and H3K27M DMGs show high expression in astrocyte-like tumor cells
Both H3K27M mutations and EZHIP overexpression lead to a global increase in H3K27 acetylation (H3K27ac), a mark associated with active transcription [18, 22, 24,25,26,27]. Moreover, H3K27M DMGs and PFAs exhibit unique H3K27ac marked enhancer and super enhancer profiles [42, 64, 65]. We combined information from gene lists generated by Mack et al. and Krug et al. and reanalyzed publicly available H3K27ac ChIP-seq data from these studies to identify enhancers that are shared by, or unique to, each tumor (Additional file 1: Fig. S3). H3K27ac-marked gene enhancers shared by both H3K27M DMGs and PFAs (n = 533, Additional file 1: Fig. S3) were mainly associated with axonogenesis, axon guidance, and cell motility pathways, as well as differentiation of glial and oligodendrocyte lineages (Fig. 4a). DMG-specific enhancers mapped mainly to genes enriched for synaptic signaling (Fig. 4b), whereas PFA-specific enhancers were associated with genes enriched for extracellular organization and angiogenesis (Fig. 4c).
Due to the importance of enhancer activity in regulation of cell identity, we explored whether the enhancer signatures segregated with differentiation cell states within each tumor by assessing the single-cell RNA-seq datasets [4, 6]. Single-cell RNA-sequencing in H3K27M-DMGs and PFA ependymomas has demonstrated marked intratumoral heterogeneity [4, 6]. Both tumors contain malignant cells with varying expression of stem-cell-like, and more differentiated tumor cell signatures. H3K27M DMGs contain tumor cells bearing oligodendrocyte precursor (OPC)-like, oligodendrocyte (OC)-like and astrocyte (AC)-like transcriptional signatures . Similarly, PFAs contain less differentiated neuronal stem cell (NSC)-like, glial progenitor (GP)-like, and neuronal precursor cell (NPC)-like, as well as more differentiated ependymal (Epd)-like and astroependymal (AE)-like tumor cells . Mapping the expression signatures onto tumor-cell types showed the highest average expression of shared enhancer signature within astrocyte-like cells in H3K27M DMGs and astroependymal-like and immune-reactive PFA tumor cells (Fig. 4d). Evaluation of the tumor-specific enhancer signatures by single-cell type also showed increased expression in astrocytic-like lineages for both tumor types (Fig. 4d, e). We initially hypothesized that because enhancers play critical roles in regulating cell identity, and super enhancers in particular have been associated with activation of tumor-specific transcriptional programs , the H3K27M DMG and PFA-specific enhancer signatures might associate closely with more potent progenitor-like cell types. However, our findings demonstrate that these previously identified enhancer signatures seem to more closely align with expression programs of cells within the tumor that demonstrate astrocytic lineage features.
H3K27me3-enriched CRABP1 is highly expressed in progenitor populations of both tumors
In further interrogation of the single-cell datasets, we asked whether genes with retained H3K27me3 exhibited preferential expression in single-cell defined tumor cell populations. In both tumors, cells with progenitor-like states including OPC-like tumor cells in H3K27M-DMGs, and NPC-like tumor cells in PFA ependymomas, tended to have higher expression of genes in the retained H3K27me3 signature shared by H3K27M DMGs and PFAs (Fig. 5a, b).
To further assess heterogeneity, we analyzed genes with the greatest variability in expression by comparing standard deviation values for each gene across all tumor cell types in both tumors. Expression of cellular retinoic acid binding protein 1 (CRABP1) was a clear outlier for both tumor subtypes (Fig. 5c). Moreover, CRABP1 was one of the H3K27me3 marked genes (Additional file 1: Fig. S4a) with the greatest variability in expression levels in the bulk datasets (Fig. 3b). CRABP1 is frequently dysregulated in cancer [67,68,69,70,71]. CRABP1 expression segregated strongly with OPC-like cells in DMGs (Fig. 5d) and both glial progenitor-like (GP-like) and neuronal precursor-like (NPC-like) in PFAs (Fig. 5e). We determined genes that correlate with CRABP1 expression in bulk expression to find that the HOX genes were among the strongest correlates in both tumor datasets (Fig. 5f). However, HOXA2 and HOXD3 did not demonstrate any distinct patterns of expression by cell type in DMGs, and the significantly different changes observed in PFAs were far less drastic (Additional file 1: Figure S4b, c).
The scRNA-seq datasets were integrated to further explore similarities in expression patterns of H3K27M DMGs and PFAs. Integrated dimensional reduction analyses identified 20 clusters, four of which (clusters 0, 1, 4, and 13) included large populations of both PFA- and DMG-derived cells (Arrows, Fig. 5g). The top marker gene in the second-largest cluster’s (cluster 1) was CRABP1 (Fig. 5g, Additional file 1: Fig. S4d). This cluster consisted primarily of progenitor and precursor cell states: OPC-like cells from the DMGs and GP-like cells from PFAs (Additional file 1: Fig. S4e). Moreover, assessment of single-cell populations from other pediatric brain tumors using a dataset with all four subgroups of medulloblastoma  failed to identify any populations with elevated CRABP1 expression in those tumors (Additional file 1: Fig. S4f). Overall, these data support a role for CRABP1 in specific tumor cell-states in both tumors, consistent with the hypothesis that PFA and DMG tumors may benefit from expression of similar genes to maintain progenitor states.
Common H3K27me3 signatures mirror human hindbrain brain developmental patterns
H3K27M DMGs and PFAs are proposed to arise from cells along developmental lineages in the hindbrain [4,5,6, 8, 10]. We first aimed to identify whether H3K27me3-associated genes exhibited specific patterns in normal human hindbrain development. We utilized scRNA-seq data from fetal human cerebellum ranging from 9–21 weeks post-conception published by Aldinger et al. . Overall, average expression of H3K27me3-retained genes increased in more differentiated populations of cells associated with potential cells-of-origin for DMGs and PFAs. Committed OPCs demonstrated increased signature expression compared to OPCs, and brainstem-choroid/ependymal cells had higher levels of expression than early brainstem cells (Fig. 6a). Cells of astrocytic lineage did not show a similar pattern (Fig. 6a). Because OPCs are the leading proposed cell of origin for H3K27M DMGs [4, 65], we examined oligodendroglial lineages in more detail in a second single-cell dataset of human developing cerebellum (Sepp et al., unpublished) . H3K27me3 signatures were low in progenitor cells but increased along stages of OPC differentiation with the highest expression in late-OPCs and then decreased levels in more differentiated committed OPCs and oligodendrocytes (Fig. 6b). Analysis of the astrocytic lineage cells did not yield a similar pattern (Fig. 6c).
Given its notable expression pattern in the single-cell tumor datasets (Fig. 5), we examined CRABP1 expression in the developing hindbrain utilizing a scRNA-seq dataset generated from human brain samples from the first trimester . Carnegie stages are used to define periods of human brain development . In the hindbrain, we noted a peak of CRABP1 expression in stage 19, which corresponds to the peak in expression of H3-3A, the histone variant most commonly mutated in DMGs (Additional file 1: Fig. S5a). With respect to anatomic location, CRABP1 expression was higher in the spinal cord and hindbrain vs. thalamus and cerebellum (Fig. 6d). Known areas of high retinoic acid (RA) signaling in development, including the olfactory placode and optic pathways demonstrated enrichment of CRABP1 expression, whereas expression in forebrain/cortical-related structures was variable (Fig. 6d). Similarly, CRABP1 expression was restricted to a subset of brainstem cells in the Aldinger et al. developing cerebellum atlas (Fig. 6e). At a cellular level, CRABP1 expression was highest in the progenitor cell types compared to their more differentiated oligodendrocytic and astrocytic cells (Fig. 6f).
Data from mouse development have previously identified CRABP1 expression as specific to rhombomeres 4–6 (r4–6) . The r4-6 segments give rise to the caudal portion of the basilar pons (r4) and retropontine structures (r5–6) [75, 76]. Given this spatial patterning, the Allen Brain Atlas’ Developing Human Transcriptome was examined to determine patterns of CRABP1 expression [77, 78]. Within hindbrain structures, CRABP1 expression strongly correlated with HOX genes including HOXA2 and HOXD3 (Fig. 6g). Moreover, HOXD3, HOXA2 and CRABP1 expression in the developing human hindbrain at 15-weeks gestation was mainly restricted to developing human pontine and medullary structures (Fig. 6h–j, Additional file 1: Fig. S5b), a finding supported by developing mouse brain expression patterns (Additional file 1: Fig. S5c). Altogether, these data support the positional expression patterns observed in both H3K27M DMGs and PFAs, consistent with these patterns reflecting epigenetic states from developmental lineages.
While EZHIP-expressing PFAs and H3K27-altered DMGs (including H3.3 and H3.1K27M gliomas and EZHIP positive DMGs) have distinct natural histories and histologic features, evidence of their shared molecular features continues to mount as we profile broader samples of these tumor types. Furthermore, both tumors most commonly arise in neighboring hindbrain/posterior fossa-derived structures. This pattern suggests that developmental lineages in this region possess susceptibility to alterations that impede canonical PRC2 activity and therefore regulation of H3K27me3 state. Leveraging the existing molecular knowledge about each of these tumor types, we exploited genomic, bulk transcriptomic, scRNA-seq, and epigenomic profiles of both cancers to elucidate shared features that may improve our understanding of tumor biology.
Assessment of the genomic landscape of these tumors demonstrated that H3.1K27M DMGs showed greater copy number similarity with EZHIP-expressing DMGs than did H3.3K27M tumors, with more quiescent landscapes and higher frequencies of 1q gain. These profiles of relatively lower genomic instability may point toward greater commonalities between PFAs and H3.1K27M vs. H3.3K27M DMGs. Consistent with this pattern, we identified high ACVR1—a gene frequently altered in H3.1-mutant DMGs—expression to be associated with: (a) worse survival in PFAs, and (b) upregulation of pathways that were also enriched in ACVR1-mutated gliomas.
We additionally characterized common and unique features of H3K27M DMG and PFA chromatin landscapes based on patterns of H3K27me3/ac genomic deposition. The two tumors exhibited a considerable overlap in both H3K27me3-marked and H3K27ac-enhancer-associated genes. Exploration of the transcriptional states associated with these gene sets demonstrated inter- and intra-tumoral heterogeneity of expression patterns. Common enhancer-associated genes were enriched in cell motility and oligodendrocyte and glial differentiation pathways. Moreover, scRNA-seq demonstrated the highest levels of expression of these genes in astrocytic lineage cell types, especially in comparison to progenitor cell types. These patterns highlight the unique insights single-cell resolution transcriptomics can provide and illustrate how tumor-specific enhancers generated from bulk-sequencing methods may not fully reflect the regulatory landscapes of the tumors.
Interrogation of genes associated with residual H3K27me3 also yielded important insights. The sensitivity of H3K27M DMGs and PFAs to EZH2 inhibition [33,34,35] points to a potential role for H3K27me3 in maintaining tumor cell proliferative capacity. Data from developing cerebellar atlases provide some support for this model in that upregulation of genes within these signatures is seen along oligodendrocyte lineages and in brainstem choroid/ependymal cells. Furthermore, we noted distinct expression profiles of proximal vs. distal HOX genes, which correlated with inferred anatomic origins of tumor, and these genes demonstrate distinct anterior–posterior boundaries of expression in the developing human hindbrain. These data suggest that developmental epigenetic profiles and origins of PFAs and H3K27M DMGs may influence the H3K27me3 landscape and gene expression programs in these tumors.
CRABP1, like the HOX genes, has been shown to have highly specific expression patterns in mouse nervous system development . In both PFAs and DMGs, its expression correlated with specific tumor cell types, with an enrichment among progenitor-like cells. While its precise role remains unclear, CRABP1 has been proposed to be a critical regulator of retinoic acid signaling, preventing canonical RA-induced transcriptional programs . This role is of particular interest due to retinoic acid’s function in hindbrain patterning [80,81,82,83,84] and the previous identification of the retinoic acid binding protein RXRA as the top ranked super-enhancer associated gene in H3K27M DMGs . Together these data suggest that CRABP1 may be associated with progenitor cells in early stages of the developing hindbrain that may be dysregulated in both PFAs and H3K27M DMGs. Further investigation is needed to elucidate whether CRABP1 contributes to maintenance of progenitor-type states in the presence of retinoids during development.
Overall, our studies further define molecular commonalities in the biology between DMGs and PFAs that express H3K27M and EZHIP and highlight similar patterns in the heterogeneity among tumors of each class. Moreover, some of these common epigenetic signatures showed specific patterns in human hindbrain development supporting the overall hypothesis that hindbrain epigenetic programs contribute to the biology of both tumors.
Availability of data and materials
Data were obtained as described in the methods section.
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The work was supported in part by grants from National Institute of Neurological Disorders and Stroke (NINDS) R01NS110572 (SV), National Cancer Institute (NCI) R01ACA261926 (SV), NCI R37CA214955-01A1 (AR), NCI P30CA046592 (AR), Chad Tough Defeat DIPG foundation (SV), Alex Lemonade Stand Foundation (SV), the Hyundai Hope on Wheel Foundation (SV), and the Rogel Cancer Center PIBS Graduate Student Scholarship (MP).
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Pun, M., Pratt, D., Nano, P.R. et al. Common molecular features of H3K27M DMGs and PFA ependymomas map to hindbrain developmental pathways. acta neuropathol commun 11, 25 (2023). https://doi.org/10.1186/s40478-023-01514-z