Fig. 6From: Sleep fragmentation affects glymphatic system through the different expression of AQP4 in wild type and 5xFAD mouse modelsA Representative images of immunohistochemistry of GFAP and 6e10 antibodies in all the regions analyzed. B Representative images of immunohistochemistry of GFAP and 6e10 antibodies acquired with Axioscan microscope. Scale bar 1000 μm. C Histogram of GFAP and 6e10 shown together. D Histogram of GFAP density analyzed through the percentage of pixels, after the same threshold is set for all the region of interests. NF not fragmented mice, F fragmented mice, LS lateral septum, RSC retrosplenial cortex, MSC motor-sensory cortex, DG dentate gyrus, TH thalamus, HY hypothalamus, BLA basolateral amygdala. The data are mean standard error of the mean (SEM). Each data point represents an individual animal. **p < 0.01; ****p < 0.0001 versus control by ANOVA followed by Bonferroni post-hoc test, n = 4 per conditionBack to article page