Fig. 2

Nuclear RNA-seq confirms cell type specificity in TL and TLE FANS-isolated populations. (a) RNA-seq principal component analysis reveals separation driven by sorted cell type. (b) Heatmap representations of nuclear gene expression (rld-normalized) RNA-seq data, derived from sorted PAX6 + , NEUN + , and OLIG2 + populations. Strong and selective enrichment of astrocytic, neuronal, and OPC markers is seen in each respective FANS population (normalized by row), with lack of contaminant microglial (M), endothelial (E), and pericyte / smooth muscle cell (P/SMC) gene expression in the sorted populations (normalized by column). (c) Gene set enrichment analyses (GSEA) confirm significant, cell type-specific enrichment. Rld-normalized nuclei RNA-seq data (PAX6 + , OLIG2 + , NEUN +) is analyzed against the top 500 overexpressed set of genes unique to resting human astrocytes [46] or to mouse OPCs [52]. For each gene set, each gene expression value is calculated relative to the average expression of all other populations and then sorted by highest relative expression