Fig. 5

Recurrent NF-PitNETs exhibit up-regulation of β-catenin pSer552. Immunofluorescence reveals that β-catenin pSer552 is predominantly present in the nucleus (green, white arrows in a, d) localised with DAPI (nuclear blue staining). Cultured NF-PitNET cells were negative for the monocyte marker CD44 which confirms the absence of monocytes in the culture. Images are representative of n = 3 recurrent NF-PitNET cultured tumours. i, j Western blot revealed increased phosphorylation of β-catenin pSer552 in recurrent compared to non-recurrent NF-PitNETs. i Representative image of a western blot of 6 non-invasive/non-recurrent (NI/NR) NF-PitNETs compared to 6 Invasive (I) and 6 recurrent (R) NF-PitNETs immunoblotted against β-catenin pSer552 and total β-catenin. j Quantification of the ratio between β-catenin pSer552 and total β-catenin showed a 5.7fold increase in β-catenin pSer552 in recurrent and 2.3fold increase in invasive NFPTs group compared to non-recurrent/non-invasive group. Data is represented as Mean ± SEM. Statistical significant of *p < 0.05, ****p < 0.0001 using two-tailed unpaired Student’s t-test. The western blot image is a representative image of 3 independent experiments. Scale bar in H represents 100 µm