Fig. 1

Traumatic brain injury causes early changes in mossy fiber terminals in the cerebellum. a Schematic diagram of the cortico-ponto-cerebellar circuit in a Thy1-GFP-M mouse mouse subjected to cFPI. Illustrations of mouse brains showing the area impacted by the pressure pulse at time of cFPI, the cerebellum (dorsal view) and the pontine nuclei (ventral view). b Light-sheet microscopy imaging of the cerebral hemispheres and cerebellum cleared with vDISCO shows highly detailed neuronal structure and axonal projections. c–e 3D reconstruction of cleared whole brain of a Thy1-GFP-M mouse. c Two selected representative horizontal planes at d pontine nuclei (plane 1) and e cerebellum (plane 2) are shown. f High-magnification representative fluorescence images of the pontine nuclei of a sham-injured mouse and brain-injured mice at 2, 7 and 30 dpi. g) Plots of signal intensity profiles from boxed areas in f in the pontine nuclei of a sham-injured mouse, and brain-injured mice at 2, 7 and 30 dpi. h High-magnification, representative fluorescence images of the vermis lobules IV/V of the cerebellum of a sham-injured mouse and brain-injured mice at 2, 7 and 30 dpi. i Plots of signal intensity profiles from the boxed areas in h in the vermis lobule IV/V of a sham-injured mouse and brain-injured mice cerebellum at 2, 7 and 30 dpi