Fig. 6
Changes in microglial identities post PMX205 treatment. Bar plot of the relative proportion of microglial and perivascular macrophage subtypes per genotype and treatment. Each color represents a population of cells. The x-axis contains the tissues, genotypes, and treatments. The first four columns represent hippocampus, and the last four columns represent cortex. The first two columns of each tissue (hippocampus or cortex) represent WT and the last two columns of each tissue represent Tg2576 mice. The ‘ + ’ indicates PMX205-treated mice and ‘– ‘ represents vehicle-treated mice. The y-axis indicates the proportion of microglial and PVM cells from each given cluster. B GO Bubble plot of select top pathways and biological processes enriched in microglial clusters 8, 13 and 16. Circle size represents the number of genes enriched in each GO term. C–D Heatmap of signature homeostatic, DAM1 and DAM2 genes per microglial and PVM clusters. Each row shows the normalized mean expression per cluster. The bright yellow color shows higher expression of the given genes, and the darker blue represents the lower expression of the genes. C Columns are arranged as seen in panel A. Normalized mean expression per tissue, genotype, and treatment (D)