Antigen | Catalogue Number | Source | Species | Dilution [ab conc.] | Target |
---|---|---|---|---|---|
Osteopontin (IgG2a) | ab69498 | Abcam | Mouse | 1:750 | Osteopontin |
GFAP (IgG1) | G3893 | Sigma | Mouse | 1:1000 | Astrocytes, radial glia |
Olig2 | AB9610 | Millipore | Rabbit | 1:500 | Oligodendrocytes |
Iba1 | 019–19,741 | WAKO | Rabbit | 1:1000 | Microglia |
Biotinylated secondary | BA-2001 | Vector Laboratories | Horse anti-mouse | 1:200 [1.5Â mg/ml] | Â |
ABC Elite Kit | PK6200 | Vector Laboratories | Â | 1:200 | Â |
Alexa Fluor 488 | A21131 | Life Technologies | Goat anti-mouse (IgG2a) | 1:500 [2Â mg/ml] | Â |
Alexa Fluor 546 | A21123 | Life Technologies | Goat anti-mouse (IgG1) | 1:500 [2Â mg/ml] | Â |
Alexa Fluor 546 | A11010 | Life Technologies | Goat anti-rabbit (IgG) | 1:500 [2Â mg/ml] | Â |