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Table 2 Primary and secondary antibody information

From: White matter injury but not germinal matrix hemorrhage induces elevated osteopontin expression in human preterm brains

Antigen Catalogue Number Source Species Dilution [ab conc.] Target
Osteopontin (IgG2a) ab69498 Abcam Mouse 1:750 Osteopontin
GFAP (IgG1) G3893 Sigma Mouse 1:1000 Astrocytes, radial glia
Olig2 AB9610 Millipore Rabbit 1:500 Oligodendrocytes
Iba1 019–19,741 WAKO Rabbit 1:1000 Microglia
Biotinylated secondary BA-2001 Vector Laboratories Horse anti-mouse 1:200
[1.5 mg/ml]
 
ABC Elite Kit PK6200 Vector Laboratories   1:200  
Alexa Fluor 488 A21131 Life Technologies Goat anti-mouse (IgG2a) 1:500
[2 mg/ml]
 
Alexa Fluor 546 A21123 Life Technologies Goat anti-mouse (IgG1) 1:500
[2 mg/ml]
 
Alexa Fluor 546 A11010 Life Technologies Goat anti-rabbit (IgG) 1:500
[2 mg/ml]