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Table 2 Primary and secondary antibody information

From: White matter injury but not germinal matrix hemorrhage induces elevated osteopontin expression in human preterm brains

Antigen

Catalogue Number

Source

Species

Dilution [ab conc.]

Target

Osteopontin (IgG2a)

ab69498

Abcam

Mouse

1:750

Osteopontin

GFAP (IgG1)

G3893

Sigma

Mouse

1:1000

Astrocytes, radial glia

Olig2

AB9610

Millipore

Rabbit

1:500

Oligodendrocytes

Iba1

019–19,741

WAKO

Rabbit

1:1000

Microglia

Biotinylated secondary

BA-2001

Vector Laboratories

Horse anti-mouse

1:200

[1.5 mg/ml]

 

ABC Elite Kit

PK6200

Vector Laboratories

 

1:200

 

Alexa Fluor 488

A21131

Life Technologies

Goat anti-mouse (IgG2a)

1:500

[2 mg/ml]

 

Alexa Fluor 546

A21123

Life Technologies

Goat anti-mouse (IgG1)

1:500

[2 mg/ml]

 

Alexa Fluor 546

A11010

Life Technologies

Goat anti-rabbit (IgG)

1:500

[2 mg/ml]

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