Fig. 2

MFG-E8 expression analysis in APP23 mice. a Representative western blot images and quantification of MFG-E8 protein levels in brain homogenates from 12-, 18-, and 24-month-old WT and APP23 mice and in 24-month-old plasma samples, n = 3–6/group. b Representative immunofluorescence images and quantification of the MFG-E8-positive vessels in brains from 24-month-old WT and APP23 mice. The scale bar indicates 100 μm, n = 3/group. c Representative confocal images showing the localization of MFG-E8 (green) and Aβ (red) in parenchymal brain sections (I) from 24-month-old APP23 mice. II) Representative images showing no MFG-E8 detection in parenchymal Aβ deposits. III) Representative images showing MFG-E8 detection in an Aβ-positive vessel. The scale bar indicates 10 μm. *p < 0.05, **p < 0.01, ****p < 0.0001