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Fig. 10 | Acta Neuropathologica Communications

Fig. 10

From: Protein farnesylation is upregulated in Alzheimer’s human brains and neuron-specific suppression of farnesyltransferase mitigates pathogenic processes in Alzheimer’s model mice

Fig. 10

Pharmacological inhibition of FT reverses the hyperactivation of Ras-Rheb-mTORC1 signaling in SH-SY5Y-APP695 cells. A Immunoblot analysis of FL-APP and downstream targets of Ras-Rheb-mTORC1 signaling, including ERK and ribosomal protein S6, in the lysates of SH-SY5Y-APP695 and mock-transfected control cells. B Densitometric analysis of immunoblots normalized by the amount of GAPDH or total proteins for phosphorylated proteins (P-ERK, P-Akt, P-S6) with the levels in the control group set as 100%. n = 6 replicates from 2 independent experiments. C Immunoblot analysis of farnesylation status of H-Ras and Rheb, and D phosphorylation status of downstream targets of Ras and Rheb in the lysates of SH-SY5Y-APP695 cells after treated with vehicle (DMSO) or 1 μM tipifarnib for 48 h. E Densitometric analysis of immunoblots of P-ERK, P-S6K, and P-S6 normalized by the amount of total ERK, S6K, and S6 with the levels in the control group set as 100%. n = 6 replicates from 2 independent experiments. Student’s t-test, 2-tailed; *p < 0.05; ***p < 0.001; ****p < 0.0001

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Acta Neuropathologica Communications

ISSN: 2051-5960

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