Fig. 1From: Label-free vibrational imaging of different Aβ plaque types in Alzheimer’s disease reveals sequential events in plaque developmentWorkflow. Fourier-transform infrared (FTIR) and Raman imaging were applied to selected sample areas. Subsequently, the sample was immunostained against amyloid beta (Aβ) and imaged with light microscopy. The resulting (spectral) images were spatially aligned to generate a precisely overlaid, unified dataset. An experienced neuropathologist (B.D.C.B) annotated Aβ-IHC images to the different plaque types. Based on this data, spectral analysis was conducted and statistically evaluatedBack to article page