Fig. 3
From: P53 aggregation, interactions with tau, and impaired DNA damage response in Alzheimer’s disease
p53 interacts with tau in human brain; tau oligomers interact with p53 in AD brain. a Representative confocal images of human AD and age-matched control brain immunofluorescently probed with anti-p53 (red) and anti-tau (green). b Magnified ROI from AD merged panels in A. Large peri-nuclear colocalization between p53 and tau is shown in AD brain with other tau in the vicinity. c Colocalized pixel map of signal and intensity scatterplot with strong PCC (0.83–86). d Magnified ROI from control merged panels in A. (1) Diffuse p53 signal with very little colocalization shown. (2) Small single peri-nuclear region of colocalization detected in control brain. e Representative confocal images of human AD and control brain using PLA with anti-p53 and anti-tau (Tau13 Biolegend). f Magnified ROI from AD merged panel in E shows large peri-nuclear interaction. g Magnified ROI from control merged panel in E shows single small region of colocalization similar to magnified image in D. h Fluorescent intensity of p53-tau from PLA shows significant increase in p53-tau interaction in AD brain (n = 2; in technical triplicate). i Representative immunofluorescence confocal images of human AD and control brain probed with anti-p53 (red) and anti-T22 (tauO specific; green). j Magnified ROI from AD merged panels in I. Large peri-nuclear colocalization between p53 and tauO are shown in AD brain with other tau in the vicinity. k Orthogonal view of magnified ROI in J shows a large amount of p53 is localized outside the nucleus where it heavily colocalizes with T22. TauO is also observed inside the nucleus by orthogonal view. l Magnified ROI from control merged panel in I. p53 is observed in the nucleus and no tauO are observed in control brain. m Fluorescent intensity of T22 shows significantly more tau oligomers in AD brain (n = 3 in technical triplicate). Keyence Microscope 60X-100X magnification. Scale bar = 50 μm