Fig. 2

Lipid-specific IgM induces anti-viral transcriptional signature in vitro. a, b Microarray analysis of rat myelinating cultures treated 24 h with media alone, IgM from mouse myeloma (IgM, Sigma) or O4. a Cluster analysis of all significant genes (FDR p-value < 0.05); heat map shows standardized gene expression level of each gene with high expression in red and low expression in blue. b Bar graph showing genes most upregulated by O4, interferon stimulated genes denoted by an asterisk. c-g RT-qPCR analysis of rat cultures treated with media alone, IgM, A4CD or O4 for c 4 h and d, g 24 h. Data analysed by one-way ANOVA and significance determined by Tukey’s post hoc test. IgM (n = 4), A4CD (n = 3) and O4 (n = 4). Significant differences denoted as *p < 0.05, **p < 0.01 and ****p < 0.0001