Fig. 8

Immunohistochemical analysis of 5-Lox expression in the hippocampus. a 5-Lox (red, monoclonal 5-Lox antibody from Abcam #ab169755) was primarily expressed in neurons of the granular cell layer but did also co-localize with some Iba1 (white) positive cells (arrow or inserts). b We analyzed the percentage (%) of 5-Lox⁺ cells in the Iba1⁺ cell population revealing increased numbers of 5-Lox + microglia in APP-PS1 mice compared to WT animals. 5-Lox⁺ microglia numbers were reduced in APP-PS1 + PLX5622 animals. c The overall 5-Lox staining (% area stained by 5-Lox) was increased in APP-PS1 mice compared to WT animals and reduced upon microglia depletion in WT + PLX5622 mice. Remaining microglia in PLX5622 treated mice displayed altered cell morphology. One-way analysis of variance with Tukey’s multiple comparison test (b, c) and unpaired t test comparing WT vs. WT + PLX5622 (c) was used. P-values < 0.05 were considered significant. Data are shown as mean with SD. Dapi was used as nucleus stain. Scale: 100 μm and 20 μm inserts (a)