Apolipoprotein E and clusterin inhibit the early phase of amyloid-β aggregation in an in vitro model of cerebral amyloid angiopathy

Endo, Yoshinori; Hasegawa, Kazuhiro; Nomura, Ryo; Arishima, Hidetaka; Kikuta, Ken-ichiro; Yamashita, Taro; Inoue, Yasuteru; Ueda, Mitsuharu; Ando, Yukio; Wilson, Mark R.; Hamano, Tadanori; Nakamoto, Yasunari; Naiki, Hironobu

doi:10.1186/s40478-019-0662-1

Table 2 Proteins in the cerebral blood vessels of CAA patients vs. non-CAA patients

From: Apolipoprotein E and clusterin inhibit the early phase of amyloid-β aggregation in an in vitro model of cerebral amyloid angiopathy

Accession number	Protein	CAA (n = 6)^a		Non-CAA (n = 5) ^a		p value*
Accession number	Protein	% Detection	NSAF_CAA	% Detection	NSAF_non-CAA	p value*
P02649	Apolipoprotein E	100	0.1259	20	0.0006	0.004
P05067	Amyloid beta A4 protein	100	0.0685	0	ND	0.004
P10909	Clusterin	100	0.039	20	0.0015	0.004
P02768	Serum albumin	100	0.046	100	0.0154	0.017
P08123	Collagen alpha-2(I) chain	100	0.0031	80	0.0019	0.329
P68871	Hemoglobin subunit beta	100	0.1695	100	0.249	0.662
P69905	Hemoglobin subunit alpha	100	0.0778	100	0.0718	0.792
P04004	Vitronectin	83	0.0085	20	0.0004	0.03
P0C0L4	Complement C4-A	83	0.0032	20	0.0001	0.03
P08670	Vimentin	83	0.0207	80	0.0159	0.247
P41222	Prostaglandin-H2 D-isomerase	67	0.013	40	0.0021	0.177
Q15149	Plectin	67	0.0006	40	0.0001	0.177
Q8IYA6	Cytoskeleton-associated protein 2-like	67	0.0147	80	0.006	0.537
P06727	Apolipoprotein A-IV	50	0.0096	0	ND	0.177
Q70EL1	Inactive ubiquitin carboxyl-terminal hydrolase 54	50	0.0035	0	ND	0.177
P11047	Laminin subunit gamma-1	50	0.0014	0	ND	0.177
P02042	Hemoglobin subunit delta	50	0.0313	80	0.0735	0.247
Q8N413	Solute carrier family 25 member 45	50	0.0102	20	0.0011	0.329
P35625	Metalloproteinase inhibitor 3	50	0.0084	20	0.0009	0.329
P07437	Tubulin beta chain	50	0.0061	40	0.0018	0.429
P12814	Alpha-actinin-1	50	0.0015	40	0.0005	0.429
P14136	Glial fibrillary acidic protein	50	0.0265	60	0.0116	0.662
Q9BQE3	Tubulin alpha-1C chain	50	0.0051	60	0.0018	0.662
A6NNT2	Putative uncharacterized protein C16orf96	50	0.004	60	0.0011	0.662
P04350	Tubulin beta-4A chain	50	0.0064	80	0.0045	0.792
P98160	Basement membrane-specific heparan sulfate proteoglycan core protein	50	0.0004	60	0.0004	1

Proteins which show the % detection to be ≥50% in CAA patients are listed. % Detection means the relative number of patients positive for each protein in both CAA and non-CAA patients
ND not detected
*The Mann-Whitney U test was used for comparisons between NSAF_CAA and NSAF_non-CAA values
^aProtein abundance values were estimated using NSAF (normalized spectral abundance factor) normalizaiton
Boldface highlights the proteins which were significantly upregulated in the cerebral blood vessels of CAA patients as compared to non-CAA patients

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ISSN: 2051-5960

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