Fig. 6

Comparison of the effect of apoE3 with apoE4 on the kinetics of early-phase Aβ(1–40) aggregation. The reaction mixture containing 5 μM Aβ(1–40), 10 μl suspensions of Matrigel-coated beads, 0.3 mg/ml HSA, PBS-NaN₃, 5 μM ThT, and 0 to 0.2 μM apoE3 or apoE4 was incubated at 37 °C with rotation at 1 rpm in the absence of an air-water interface (18 replicate wells in each case). As a positive control, the reaction mixture containing 5 μM Aβ(1–40), 0.3 mg/ml HSA, PBS-NaN₃, and 5 μM ThT was also incubated at 37 °C with rotation at 1 rpm in the presence of an air-water interface (18 replicate wells) (Air). The kinetics of early phase aggregation was analysed by the Kaplan-Meier survival method using the initiation time of fibril growth kinetics as the event of interest (see Fig. 2). The P value was less than 0.05 for all pair-wise comparisons except those between apoE3 and apoE4 when both were at either 0.05 μM or 0.2 μM. This data is representative of three independent experiments