Fig. 3

Effects of apoE and CLU on the seeded aggregation of fAβ(1–40) in vitro. a The reaction mixture containing 2.5 μg/ml fAβ(1–40), 5 μM Aβ(1–40), 0 (●), 0.05 (○), 0.1 (■), 0.2 (□), 0.5 μM (▲) apoE3, 0.3 mg/ml HSA, PBS, and 5 μM ThT was incubated at 37 °C without shaking in a 96-well plate. b The reaction mixture containing 2.5 μg/ml fAβ(1–40), 5 μM Aβ(1–40), 0 (●), 0.05 (○), 0.25 (■), 0.5 (□), 1.0 μM (▲) CLU, 0.3 mg/ml HSA, PBS, and 5 μM ThT was incubated at 37 °C without shaking in a 96-well plate. ThT fluorescence was monitored as described in Materials and Methods. Points and bars represent mean + S.D. of 6 replicates. This data is representative of three independent experiments