Fig. 2

ATP is required to suppress proliferation of mature SC. (a) Apyrase, which degrades ATP, was administered every 4 h for 36 h IM. This resulted in increased Ki67+ cells compared to inactivated apyrase controls (n = 5active/6inactive P < 0.0001). (b) In tissue sections, EdU+ Krox20+ cells were present after Apyrase treatment, and many were associated with MBP+ myelin sheaths (b, c) and S100+ myelinating Schwann cell cytoplasm (scale bar = 3 μm) (c). In c, the EdU+ SC nucleus (white) adjacent to S100+ SC cytoplasm, appears to be in telophase. (d) Animals treated twice daily with EdU during 4 days of BupOH exposure and analyzed on Day 4 showed increased EdU+ cells over sham (n = 4/group < 0.01). Animals from this cohort that were sacrificed 3 days after the final dose of EdU (Day 7) showed fewer EdU+ cells (p < 0.05). (e) Broad identification of the total EdU counts reflected from the experiments represented as a percentage of total EdU. (f) Percentage of total EdU+ cells that co labeled with Krox20. (g) Percentage of total Edu + cells that co labeled with Iba1. (h) Confocal images of teased nerves from BupOH treated mice, top shows an EdU+ cell closely associated with an MBP+ myelin sheath and S100+ cytoplasm; bottom shows an EdU+;S100β + cell apparently separating from an adjacent MBP+ fiber