Fig. 1

Radiation-associated DIPGs define a distinct molecular subtype with poor prognosis. a Immunohistochemistry performed for case 3 showed wild-type status for histone H3 (H3K27M) with retention of H3K27me3, as well as diffuse GFAP expression, which was negative in primary medulloblastoma (not shown). Positive and negative controls are shown in Additional file 1: Figure S1. Insufficient tissue was available for such analysis in cases 1 and 6; however, H3 wild-type status was demonstrated by tumor sequencing in these cases. All scale bars are 50 μm. b OS data for primary DIPG in the IDIPGR (n = 428) was compared via Kaplan-Meier analysis to OS of radiation-associated DIPG cohort (n = 8). OS was significantly less for the radiation-associated DIPG group (p = 0.046). c OS data for primary DIPG patients with both genomic and OS information available (n = 38), as categorized by histone mutational status and compared via Kaplan-Meier analysis to OS of radiation-associated DIPG cohort (n = 8). The radiation-associated DIPG cohort showed the shortest OS in comparison to the two subgroups of primary DIPG with significantly shorter survival compared to H3.3 K27 M mutant (p = 0.038) and H3.1 K27 M mutant (p = 0.024) primary DIPGs