Fig. 7
From: The chronically inflamed central nervous system provides niches for long-lived plasma cells
Expression of APRIL in inflamed mouse CNS. Mice were immunized and boosted (day 28) with rhMOG. Analysis of the spinal cords was performed at time points as indicated. a The fluorescence signal of DAPI (blue), APRIL (green) and kappa (κ, red) is shown. Magnified insets of the framed area are shown in the right corner. To determine APRIL expression in control CNS, the spinal cord of mice immunized with complete Freund’s adjuvant and Mycobacterium tuberculosis was stained (lower panel). Three to four mice from two independent experiments were analyzed. Scale bars represent 50 μm. b The mice received EdU for 14 days via drinking water after the boost (day 28). DAPI (blue), kappa (κ, green), EdU and APRIL staining were performed three weeks after stopping the EdU feeding. Four mice from two independent experiments were analyzed. Scale bar represents 20 μm