Fig. 6
From: The chronically inflamed central nervous system provides niches for long-lived plasma cells
Plasma cell niche signals CXCL12 and VCAM-1 persist in chronically inflamed mouse CNS. Mice were immunized and boosted (day 28) with rhMOG. Analysis of the spinal cords was performed at different time points as indicated. a Histology staining was performed with DAPI (blue), anti-CXCL12 (red), anti-GFAP (green) and anti-kappa (κ, right lower panel green) antibody. To determine CXCL12 expression in control CNS, the spinal cord of mice immunized with complete Freund’s adjuvant and Mycobacterium tuberculosis, was stained. Only minimal CXCL12 immunoreactivity was detected in the parenchyma. Three to five mice from two independent experiments were analyzed. Scale bars represent 100 μm in the upper panel and 50 μm in the lower panel. b DAPI (blue) and VCAM-1 (green) was stained in spinal cord sections of EAE (upper panel) and control mice (lower panel). Three mice from two independent experiments were analyzed per disease stage. Scale bars represent 50 μm