Fig. 1
Altered Ca2+ homeostasis in sCJD. a Heat map analysis of regulated Ca2+ related genes in the cortical region of the tg340-PRNP129MM sCJD mice at 120 (pre-clinical) and 180 (clinical) days after inoculation with sCJD MM1 brain homogenates compared with control inoculated mice. Data were generated by RNA-sequencing analysis as indicated in material and Methods section. Regulated genes were considered those with log2FC superior or equal to 0.5 and p value <0.05. b qPCR validation of selected genes involved in Ca2+–dependent cellular responses at 120 dpi and 180 dpi in the sCJD infected tg340 mice. Four to five animals were analysed per time point and condition. c Western-blot (n = 14/group) and (d) immunohistochemistry validation in human sCJD MM1 cases from selected proteins regulated in the sCJD infected tg340 mice. Unpaired t-test (95% CI) was used for the comparisons of the two groups. *p < 0.05; **p < 0.01; ***p < 0.001