Fig. 4
From: Tamoxifen induces cellular stress in the nervous system by inhibiting cholesterol synthesis
TAM (TAM) induces transient ATF3 upregulation and recombination in the DRG. a (left), tdtomato and ATF3 expression from a cervical DRG, 4 days after a single 75 mg/kg TAM injection (single 1 μm-thick confocal plane). a (right), ATF3 expression in the trigeminal ganglion at various timepoints following TAM (single optical planes). The inset in shows quantitative differences in the number of ATF3-positive nuclei per 400 × 400 μm single optical section. Each point represents a single image (four each from three animals per timepoint). b The proportion of neurons undergoing recombination after TAM does not increase with repeated daily dosing. Main panels are 1 μm-thick optical sections, insets are 30 μm-thick stacks from the same fields of view. The far-right image is from a TAM-treated advillin-CreERT2:stopfl/fltdtomato mouse, in which recombination occurs in nearly all DRG neurons. c Size-frequency distribution analysis reveals that after TAM treatment, recombined neurons tend to be larger (there is a rightward shift in the distributions p < 0.05, Kolmogorov-Smirnov goodness-of-fit test). SFO: sunflower oil, the TAM vehicle