Fig. 4

Bone marrow (BM) transfer experiment. a 12 month-old PSAPP mice (immunophenotype H2^b, CD45.2) were lethally irradiated with two doses of 600 rads, then reconstituted with 5 x 10⁶ BM cells from either Rag2 ko or WT donor mice (immunophenotype H2^b, CD45.1). b 6 months after transplantation, Rag2 ko BM recipients showed a fivefold reduction in B cells and CD4+ T cells, as determined by FACS staining of CD45+ blood leukocytes with antibodies against CD4 and the pan B-cell marker B220. **p < 0.01. ***p < 0.001 . n = 9–10 per group