Assessment of intensity and distribution of BSE-associated punctate PrP
deposits in the brain. The amount of BSE-associated punctate PrPSc deposits was were assessed semiquantitatively from 0–4 in 27 neuroanatomical areas of clinically positive VM mice challenged with BSE/scrapie mixtures and the scores were plotted against the areas. The brain areas scored were: 1, medulla; 2, locus coeruleus; 3 cerebellar pedunculi; 4, cochlear nuclei; 5, dorsal cerebellum (granular layer); 6, inferior colliculus; 7, superior colliculus; 8, white matter in the mesencephalic tegmentum; 9, medial geniculate nuclei; 10, peri-acquaductal grey matter; 11, Raphe nuclei; 12, red nuclei; 13, substantia nigra; 14, interpeduncular nucleus; 15, hypothalamus; 16, lateral hypothalamus; 17, zona incerta; 18, anterior dorsal thalamic nuclei; 19, laterodorsal thalamic nuclei; 20, lateral thalamus; 21, thalamus; 22, habenular bodies; 23, hippocampus; 24, septal nuclei of the paraterminal body; 25, nucleus of the vertical limb of the diagonal band; 26, medial preoptic band; 27, ventral pallidum. The content of BSE in the BSE/scrapie mixtures was 50% (brown line), 10% (amber line), 2% (red line) and 1% (blue line). Error bars indicate SEM.