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Table 1 Parameters and procedures applied to estimate the volume of brain subdivisions and number of neurons

From: Stereological study of the neuronal number and volume of 38 brain subdivisions of subjects diagnosed with autism reveals significant alterations restricted to the striatum, amygdala and cerebellum

Structures and their subdivisions Number of sections examined (per case) Obj. (Vol.) Obj. (Numb.) Grid size (μm) for fractionator Test area frame × height (μm) Number of counting spaces (per case) Number of neurons counted (per case) CE*
Amygdala (four nuclei) 12 1.25x 40x 1000 × 1000 80 × 80 × 10 356 454 0.01
Thalamus 6 1.25x 40x 1000 × 1000 80 × 80 × 10 188 313 0.01
Entorhinal c.         
LII 6 2.5x 40x 400 × 400 60 × 60 × 30 113 164 0.003
LIII 6 2.5x 40x 800 × 800 60 × 60 × 30 118 277  
LV 6 2.5x 40x 600 × 600 60 × 60 × 30 62 161  
LVI 6 2.5x 40x 800 × 800 60 × 60 × 30 68 184  
CA1 14 2.5x 40x 1000 × 1000 60 × 60 × 30 207 543 0.003
CA2 14 2.5x 40x 400 × 400 60 × 60 × 30 121 337  
CA3 14 2.5x 40x 400 × 400 60 × 60 × 30 144 330  
CA4 14 2.5x 40x 600 × 600 100 × 100 × 30 130 429  
Caudate nucleus 9 2.5x 40x 1400 × 1400 60 × 60 × 30 270 751 0.04
Putamen 9 2.5x 40x 1400 × 1400 60 × 60 × 30 309 844
Globus pallidus 6 2.5x 40x 300 × 300 80 × 80 × 30 1953 505
Nucleus accumbens 6 2.5x 40x 700 × 700 60 × 60 × 30 202 749
Magnocellular LGB 4 5x 40x 500 × 500 100 × 100 × 30 96 230 0.002
Parvocellular LGB 4 5x 40x 500 × 500 100 × 100 × 30 134 1,418 0.002
Claustrum 13 1.25x 40x 250 × 250 60 × 60 × 10 274 318 0.01
Substantia nigra 10 2.5x 40x 500 × 500 80 × 80 × 30 366 493 0.002
Magnocellular basal complex (Ch1-Ch4) 16 2.5x 40x 500 × 500 80 × 80 × 30 323 507 0.005
Dentate nucleus 11 2.5x 40x 1000 × 1000 180 × 180 × 30 335 792 0.002
Inferior olive 8 2.5x 40x 1000 × 1000 180 × 180 × 30 213 364 0.002
Cerebellum 8 1.25x 20x 1800 × 1800 180 × 180 × 30 1419 773 0.05
  1. Number of sections examined = mean number of equidistant sections examined per structure/case.
  2. Obj. (Vol.) = Objective lens (1.25x, 2.5x, or 5x) used to provide final magnification for planimetry and volumetry.
  3. Obj. (Numb.) = Objective lens (40x or 20x) used for fractionator to estimate neuronal density and total number of neurons.
  4. *CE = the average predicted Coefficient of Error of the neuronal counts (Scheaffer).