Figure 3

LSG expression in Type II FCD (severe area). Typical LSG expression in a case of Type IIa FCD (occipital lobe) histopathologically characterised by a severe lesion with an adjacent perilesional area (a, SMI ir). b: Example of Er81 pattern showing intense expression in layer V of the perilesional cortex (arrowheads), and less intense and dispersed expression in the core of the lesion. c-e: Single ISH images of each gene in the severe lesion converted to pseudo-colour images (green for Cux2, fuchsia for Rorβ, yellow for Er81, and red for Nurr1) for overlay representation. The cortical distributions of normal-looking neurons (NNs, d) and DNs (e) are shown separately, and juxtaposed with normal cortex for purposes of comparison (c). Note the normal laminar distribution and reduced number of NNs in comparison with the dispersion of DNs. f-j: Adjacent sections from the same case as that shown in a (SMI ir) and f (thionin staining), showing the expression of Cux2 (g), Rorβ (h), Er81 (i) and Nurr1 (j) mRNAs in normal-looking neurons and DNs. k-r: Details of the regions marked with asterisks in f-j showing the morphological features of the NNs and DNs. Scale bars: 5.2 mm (a, b); 240 μm (c-e); 195 μm (f-j); 50 μm (k-r) .