Figure 4

Colocalization analysis of MARK3 and MARK4 with GVD-marker CK1δ. Double-immunofluorescence with (A) MARK3 (FITC) or (B) MARK4 (FITC) and GVD-marker CK1δ (Alexa Fluor 594, red) in GVD-containing neurons in the CA1-CA2 area. Images are compressions of a Z-stack (first three images) and a 3D-reconstruction of a Z-stack imaged by confocal microscopy showing colocalization in the GVD indicated by arrowheads. Bar graphs show the percentage of intracellular granules that were single stained with MARK3 (MARK3⁺), MARK4 (MARK4⁺) or CK1δ (CK1δ⁺), or double-stained with MARK3 and CK1δ (MARK3⁺CK1δ⁺) or MARK4 and CK1δ (MARK4⁺ CK1δ⁺), as well as the average size of these granules. MARK4⁺ granules were significantly larger than MARK4⁺CK1δ⁺ and CK1δ⁺ granules. MARK3⁺, MARK3⁺CK1δ⁺ or CK1δ⁺ granules size was not significantly different. Data depict means ± SEM. *p < 0.05, by 1-way ANOVA with Bonferroni’s Multiple Comparison test, (n = 4–6 cases). Scale bars: 10 μm.