Figure 1

Characterization of MARK 1–4 mRNA expression by in situ hybridization in the hippocampus. In situ hybridization towards each individual isoform of MARK in fresh-frozen sections of hippocampus from two representative NDE cases (A, #6 and B, #7) and two representative AD cases (C, #27 and D, #29), revealing expression and strong overlap of all four isoforms in the dentate gyrus (black arrow head) and all CA fields. The CA2 field is denoted with red arrowheads in the MARK2 panel. Sections hybridized with sense-transcribed probes were used as negative control, with a representative picture presented in the inset. (E) Photomicrograph of emulsion-dipped sections counterstained with hematoxylin from the case in A, from the area indicated by the box in A. Scale bars: (A) 1 mm; (E) 100 μm.