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Figure 1 | Acta Neuropathologica Communications

Figure 1

From: Extensive deamidation at asparagine residue 279 accounts for weak immunoreactivity of tau with RD4 antibody in Alzheimer’s disease brain

Figure 1

Immunoblot and LC/MS/MS analyses showed a lower immunoreactivity of RD4 with tau in AD and deamidation at N279. Immunoblot analysis of Sarkosyl-insoluble (a, b) and soluble (c, d) tau from AD, PSP and CBD brains (three cases for each disease) with anti-tau monoclonal antibodies T46 (a, c) and RD4 (b, d). Arrows indicated the positions of the 60, 64, 68 kDa triplet tau bands in AD brains, and arrowheads indicate the ~33 and ~37 kDa C-terminal fragments that distinguish PSP and CBD. Identification of deamidated amino acid residue by nano-electrospray tandem mass spectrometry (e). Product ion spectrum of a mass signal of tryptic peptide VQIINK detected in Sarkosyl-insoluble tau from AD brain, showing the b and y ion series. These results identify the site of deamidation as N279, indicated by N*.

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Acta Neuropathologica Communications

ISSN: 2051-5960

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