Early administration of DAF decreases C3 and C5b-9 deposition in brain tissue from BOP exposed animals. Representative photomicrographs of brain frozen sections were stained with anti-C3/3a and C5b-9 antibodies (top), and the total fluorescence of C3 and C5b-9 deposition (bottom). Frontal grey matter ( a and c ) and hippocampus (DG) ( b and d ). Original magnification of × 200. Scale bar, 200 μm. Group data is expressed as mean ± SEM and compared using one-way ANOVA followed by Bonferroni test. n = 8 for control, 3 and 24 h experimental groups. n = 5 for 48 h experimental groups.